The mutational background in colorectal carcinogenesis

Research Project

Project/Area Number	16K14607
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Tumor biology
Research Institution	Kyoto University
Principal Investigator	OGAWA SEiSHi 京都大学, 医学研究科, 教授 (60292900)
Co-Investigator(Renkei-kenkyūsha)	SAKAI Yoshiharu 京都大学, 医学研究科, 教授 (60273455) MARUSAWA Hiroyuki (YOSHIDA Kenichi) 京都大学, 医学研究科, 講師 (80324630)
Project Period (FY)	2016-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywords	大腸癌 / 遺伝子変異 / 発がん / 大腸
Outline of Final Research Achievements	The incidence of colorectal cancer is increasing in Japan. A lot of mutations are acquired during carcinogenesis, the mechanism of which, however, have been poorly understood. In this study, we isolated single crypts from normal colon mucosa obtained from patients with colorectal cancer or adenoma and healthy volunteers. Because a single crypt consists of monoclonal cells, we performed whole-exome sequencing of single crypts to detect somatic mutations at stem cell level. The number of mutations in a crypt increased with age. The mutational signature was the same as age-related mutational signature which have been recently reported in cancer genome analyses. These results reveal that somatic mutations accumulate in normal colon mucosa along with aging. Our study provides novel insights in the carcinogenesis of colorectal cancer.

Report

(2 results)

2016 Annual Research Report Final Research Report ( PDF )

Research Products

(1 results)

All Presentation (1 results) (of which Int'l Joint Research: 1 results)

[Presentation] The carcinogenic sequence in colitis-associated cancer/潰瘍性大腸炎関連大腸癌の発癌機序2016
- Author(s)
  Nobuyuki Kakiuchi, Kenichi Yoshida, Yusuke Shiozawa, Kenichi Chiba, Yuichi Shiraishi, Takaki Sakurai, Yoshiharu Sakai, Motoi Uchino, Seiichi Hirota, Hiroki Ikeuchi, Satoru Miyano, Hiroyuki Marusawa, Seishi Ogawa
- Organizer
  第75回日本癌学会学術総会
- Place of Presentation
  横浜
- Related Report
  2016 Annual Research Report
- Int'l Joint Research