Innovative development of DDS for microRNA therapeutics by an application of anti-PCSK9 antibody
| Project/Area Number |
16K14630
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor therapeutics
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
INAZAWA Johji 東京医科歯科大学, 難治疾患研究所, 教授 (30193551)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | がん / マイクロRNA / 核酸抗がん薬 / DDS / 脂質ナノ粒子 / PCSK9 / LDLR / LNP |
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| Outline of Final Research Achievements |
Tumor-suppressive microRNAs (TS-miRs) target multiple cancer-promoting genes concurrently and may be useful as a therapeutic agent for cancer therapy. The development of drug delivery system (DDS) is critical for the implementation of miR-based therapeutics. miR-X we identified effectively induces apoptosis by concurrently directly targeting multiple genes associated with cytoprotective mechanisms in esophageal cancer cells. In this project, we investigated therapeutic potential of the lipid-nanoparticle (LNP)-mediated DDS for miR-634-based cancer therapy. We confirmed the enforced expression of miR-X to be able to induce the apoptosis in various cancer cell lines. In xenograft tumors of BxPC3 cells, a pancreatic cancer cell line, in mice, the intravenous administration of the LNP incorporated miR-X significantly induced tumor growth inhibition. These results suggest therapeutic utility of LNP-mediated DDS for TS-miR(s) to cancer cells.
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Report
(2 results)
Research Products
(29 results)
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[Journal Article] GWAS identifies two novel colorectal cancer loci at 16q24.1 and 20q13.12.2018
Author(s)
Tanikawa C, Kamatani Y, Takahashi A, Nagayama S, Mimori K, Mori M, Yasuda J, Tsuboi A, Shimizu A, Sasaki M, Yamaji T, Tsugane S, Naito M, Wakai K, Koyama T, Takezaki T, Yuji K, Murakami Y, Nakamura Y, Matsuda K, et al.
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Journal Title
Carcinogenesis
Volume: Epub
Pages: 652-660
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The Japanese Society of Pathology Guidelines on the handling of pathological tissue samples for genomic research: Standard operating procedures based on empirical analyses2018
Author(s)
Kanai Y, Nishihara H, Miyagi Y, Tsuruyama T, Taguchi K, Katoh H, Takeuchi T, Gotoh M, Kuramoto J, Arai E, Ojima H, Shibuya A, Yoshida T, Akahane T, Kasajima R, Morita KI, Inazawa J, Sasaki T, Fukayama M, Oda Y.
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Journal Title
Pathol Int
Volume: 68
Pages: 63-90
DOI
NAID
Related Report
Peer Reviewed / Open Access
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