• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A novel principle for integrative analysis of nucleosome centers and transcription factor-binding sites

Research Project

Project/Area Number 16K14642
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Genome biology
Research InstitutionKyushu University

Principal Investigator

Ito Takashi  九州大学, 医学研究院, 教授 (90201326)

Research Collaborator UMEYAMA Taichi  九州大学, 大学院医学研究院, 学術研究員 (30706370)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywordsゲノム / ヌクレオソーム / クロマチン / 転写因子 / 次世代シーケンサー / DMS / ヌクレオソーム中心 / DMS-seq / DNA形状 / ゲノムフットプリンティング
Outline of Final Research Achievements

The cells in our body select distinct subsets of genes in the genome, thereby generating a variety of unique cell types and responding appropriately to changes in the environment. Since the selection is done by the transcription factors and nucleosomes bound to the genomic DNA, it is critical to comprehensively reveal their binding sites for an in-depth understanding of genome functions. We have developed a novel method termed DMS-seq using dimethylsulfate, a cell-permeable DNA-modification reagent, and next-generation sequencer. DMS-seq has, for the first time, enabled comprehensive identification of transcription factor-binding sites and the center positions of nucleosomes without nuclear isolation and genetic manipulations. We expect that DMS-seq provides a unique method to accelerate our understanding of genomic regulation.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (3 results)

All 2017 2016 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] DMS-Seq for In Vivo Genome-wide Mapping of Protein-DNA Interactions and Nucleosome Centers.2017

    • Author(s)
      Umeyama T, Ito T
    • Journal Title

      Cell Reports

      Volume: 21 Issue: 1 Pages: 289-300

    • DOI

      10.1016/j.celrep.2017.09.035

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] DMS-seqによる蛋白質-DNA相互作用とヌクレオソーム中心のゲノムワイドなin vivoマッピング2016

    • Author(s)
      梅山大地、伊藤隆司
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-12-02
    • Related Report
      2016 Research-status Report
  • [Remarks] 細胞内におけるタンパク質-DNA相互作用の全体像を捉える新しい方法を開発

    • URL

      http://www.kyushu-u.ac.jp/ja/researches/view/185

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi