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Molecular basis of activation of plant immune system by atomasphic plasma irradiation and its application to plant disease control

Research Project

Project/Area Number 16K14858
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant protection science
Research InstitutionTohoku University

Principal Investigator

Takahashi Hideki  東北大学, 農学研究科, 教授 (20197164)

Co-Investigator(Kenkyū-buntansha) 金子 俊郎  東北大学, 工学研究科, 教授 (30312599)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords大気圧プラズマ / 植物病原体 / 活性酸素種 / 活性窒素種 / 植物病害防除 / 植物免疫 / タバコモザイクウイルス / 病害抑制 / 植物病理
Outline of Final Research Achievements

When rice seeds were irradiated with atmospheric plasma, the occurrence of "Bakanae disease" and bacterial damping-off disease in rice seedlings was suppressed. Irradiation of Arabidopsis thaliana with plasma reduced the level of virus multiplication. It has been known that reactive oxygen species act as a signaling molecule for plant immune system. Interestingly, the comprehensive analysis of reactive molecules produced by atmospheric plasma irradiation indicated that peroxynitrite (HOONO) and pernitric acid (HOONO2), which have antimicrobial activity, and N2O5 accumulated in gas phase, and NO3- was detected in liquid phase. Furthermore, in plants irradiated with atmospheric plasma, defense-related gene expression: PR1, PDF1.2 and ChiB, was significantly induced, suggesting that plant immune system seems to be activated by irradiation of plants with atmospheric plasma.

Academic Significance and Societal Importance of the Research Achievements

プラズマを植物種子や葉に照射することにより、殺菌作用や植物免疫を介して病気の発生を抑制できることが明らかになった。これらの知見は、プラズマを活用することにより、農薬のみに依存しない病害防除と安全・安心な農産物の生産の実現や、農薬の使用を削減することによる環境保全への貢献が期待できる。
さらに、プラズマ照射により生成される活性分子種が明らかになったことは、プラズマ照射が植物の生育に及ぼす影響(効果)を明らかにする上で、重要な知見を提供したことになる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results) Presentation (9 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Atmospheric-pressure plasma irradiation can disrupt tobacco mosaic virus particles and RNAs to inactivate their infectivity2018

    • Author(s)
      Hanbal Sara E.、Takashima Keisuke、Miyashita Shuhei、Ando Sugihiro、Ito Kumiko、Elsharkawy Mohsen M.、Kaneko Toshiro、Takahashi Hideki
    • Journal Title

      Archives of Virology

      Volume: 163 Issue: 10 Pages: 2835-2840

    • DOI

      10.1007/s00705-018-3909-4

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Management of bakanae and bacterial seedling blight diseases in nurseries by irradiating rice seeds with atmospheric plasma.2017

    • Author(s)
      Ochi, A., Konishi, H., Ando, S., Sato, K., Yokoyama, K., Tsushima, S., Yoshida, S., Morikawa, T. Kaneko, T. and Takahashi, H.
    • Journal Title

      Plant Pathology

      Volume: 65 Issue: 1 Pages: 67-76

    • DOI

      10.1111/ppa.12555

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 大気圧プラズマの照射条件の違いによる植物免疫誘導の変化2019

    • Author(s)
      安藤杉尋・清水浩晶・A.Shahir A.Nor・高島圭介・宮下脩平・金子俊郎・高橋英樹
    • Organizer
      令和元年度日本植物病理学会東北部会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Atmospheric-pressure plasma irradiation can disrupt tobacco mosaic virus particles and RNAs to inactivate their infectivity2019

    • Author(s)
      Sara E. Hanbal・Keisuke Takashima・Shuhei Miyashita・Sugihiro Ando・Kumiko Ito・Mohsen M. Elsharkawy・Toshiro Kaneko・Hideki Takahashi
    • Organizer
      令和元年度日本植物病理学会東北部会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 有機資材から作成したイネもみ枯細菌病抑制効果をもつ細菌集団の解析2018

    • Author(s)
      安藤杉尋・Thomas Schermer・三富直人・吉田重信・對馬誠也・宮下脩平・高橋英樹
    • Organizer
      平成30年度日本植物病理学会東北部会
    • Related Report
      2018 Research-status Report
  • [Presentation] 有機栽培畑土壌と慣行栽培畑土壌における真正細菌集団の比較解析2018

    • Author(s)
      中沖美優・宮下脩平・安藤杉尋・高橋英樹
    • Organizer
      平成30年度日本植物病理学会東北部会
    • Related Report
      2018 Research-status Report
  • [Presentation] ヒスチジン処理した宿主植物における数種病害に対する抵抗性誘導の検討2018

    • Author(s)
      高橋英樹・中保一浩・宮下脩平・安藤杉尋・瀬尾茂美
    • Organizer
      平成30年度日本植物病理学会東北部会
    • Related Report
      2018 Research-status Report
  • [Presentation] イネ有機栽培育苗土由来の培養可能細菌を混合施用した際の病害抑制効果と微生物相変動の関係2016

    • Author(s)
      藤杉尋・清水浩晶・小林隆・吉田重信・對馬誠也・伊藤豊彰・宮下脩平・高橋英樹
    • Organizer
      平成28年度日本植物病理学会東北部会
    • Place of Presentation
      コラッセ福島(福島県、福島市)
    • Year and Date
      2016-09-29
    • Related Report
      2016 Research-status Report
  • [Presentation] ネ有機栽培育苗土由来Pseudomonas属菌の混合施用による病害抑制機構の解析2016

    • Author(s)
      清水浩晶・鎗山純・宮下脩平・高橋英樹・安藤杉尋
    • Organizer
      平成28年度日本植物病理学会東北部会
    • Place of Presentation
      コラッセ福島(福島県、福島市)
    • Year and Date
      2016-09-29
    • Related Report
      2016 Research-status Report
  • [Presentation] イネ有機栽培育苗土由来Pseudomonas属菌のイネもみ枯細菌病抑制効果におけるエチレンの関与2016

    • Author(s)
      鎗山純・宮下脩平・高橋英樹・安藤杉尋
    • Organizer
      平成28年度日本植物病理学会東北部会
    • Place of Presentation
      コラッセ福島(福島県、福島市)
    • Year and Date
      2016-09-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 有機栽培育苗土から分離した糸状菌のイネ苗病害抑制効果の解析2016

    • Author(s)
      笠原雅美・宮下脩平・高橋英樹・安藤杉尋
    • Organizer
      平成28年度日本植物病理学会東北部会
    • Place of Presentation
      コラッセ福島(福島県、福島市)
    • Year and Date
      2016-09-29
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 植物病害防除剤及び植物病害の防除方法2018

    • Inventor(s)
      安藤杉尋・高橋英樹
    • Industrial Property Rights Holder
      安藤杉尋・高橋英樹
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-056255
    • Filing Date
      2018
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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