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Does caffene function as rhizosphere signal?

Research Project

Project/Area Number 16K14901
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionKyoto University

Principal Investigator

Sugiyama Akifumi  京都大学, 生存圏研究所, 准教授 (20598601)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsカフェイン / 根圏微生物 / 根圏
Outline of Final Research Achievements

In this study transgenic tomato plants expressing genes involved in the caffeine synthesis was generated. These transgenic plants synthesized caffeine and secreted as well. The effect of caffeine to the rhizosphere microbes was analyzed. More than 20 strains were isolated from rhizosphere soils of coffee plants. No of these bacteria showed caffeine metabolizing activities. The effect of caffeine to these bacterial was tested in vitro. Caffeine synthesis and secretion were also analyzed using the seedlings of coffee plants. From the genome analysis and gene expression analysis several candidate genes involved in the caffeine secretion were isolated.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] 根圏微生物のダイズ持続的生産への活用2017

    • Author(s)
      杉山暁史
    • Journal Title

      アグリバイオ

      Volume: 9 Pages: 28-31

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Caffeine fostering of mycoparasitic fungi against phytopathogens.2016

    • Author(s)
      Sugiyama, A., Sano, CM. Yazaki, K., Sano, H.
    • Journal Title

      Plant signaling & behavior

      Volume: 11 Issue: 1 Pages: e1113362-e1113362

    • DOI

      10.1080/15592324.2015.1113362

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] コーヒーノキ根圏の微生物叢解析及びカフェイン代謝菌の探索2018

    • Author(s)
      川上智、矢崎一史、杉山暁史
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コーヒーノキにおけるPurine Permeaseの発現及びカフェイン取り込み輸送解析2018

    • Author(s)
      掛川博文、士反伸和、永山秀佳、荻田信二郎、矢崎一史、杉山暁史
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コーヒーノキからのカフェイン分泌と根圏での分解動態の解析2017

    • Author(s)
      川上智、永山 秀佳、荻田信二郎、矢崎一史、杉山暁史
    • Organizer
      日本農芸化学会 2017年度大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 根圏に分泌される植物化学物質の動態と機能2017

    • Author(s)
      杉山暁史
    • Organizer
      植物化学シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Analysis of caffeine secretion from intact Coffea plants and the cell cultures2017

    • Author(s)
      Hirofumi Kakegawa, Tomo Kawakami, Nobukazu Shitan, Shuka Nagayama, Shinjiro Ogita, Kazufumi Yazaki and Akifumi Sugiyama
    • Organizer
      The 7th International Symposium for Sustainable Humanosphere (ISSH)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] コーヒーノキ根圈におけるカフェイン代謝菌の探索2017

    • Author(s)
      川上智、矢崎一史、杉山暁史
    • Organizer
      植物微生物研究会第 27 回研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コーヒーノキ根圏へのカフェイン分泌と輸送体の探索2017

    • Author(s)
      掛川博文、士反伸和、永山秀佳、荻田信二郎、矢崎一史、杉山暁史
    • Organizer
      植物微生物研究会第 27 回研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コーヒーノキからのカフェイン分泌と根圏での分解動態の解析2017

    • Author(s)
      川上智、永山秀佳、荻田信二郎、矢崎一史、杉山暁史
    • Organizer
      日本農芸化学会 2017年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コーヒーノキ根からのカフェイン分泌の解析2016

    • Author(s)
      川上 智、矢崎 一史、杉山暁史
    • Organizer
      植物微生物研究会
    • Place of Presentation
      仙台
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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