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Conversion of mitochondrial respiratory complex I to a light-driven proton pump by chemical modification

Research Project

Project/Area Number 16K14914
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bioorganic chemistry
Research InstitutionKyoto University

Principal Investigator

Miyoshi Hideto  京都大学, 農学研究科, 教授 (20190829)

Co-Investigator(Renkei-kenkyūsha) MURAI Masatoshi  京都大学, 農学研究科, 助教 (80543925)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords生物制御化学 / ミトコンドリア呼吸鎖酵素 / 複合体-I / タンパク質化学修飾 / ミトコンドリア / 化学修飾 / ミトコンドリア呼吸鎖 / 光スイッチ
Outline of Final Research Achievements

The results obtained in this work are summarized as follows.
1) This work established the chemical method to introduce a first tag to Asp160 of the 49 kDa subunit, which forms deep inside of the quinone-binding pocket, by a tosyl chemistry technique. 2) To introduce a second tag to the first tag by click chemistry technique, this work investigated reactivities of some pairs of first tag/second tag. 3) It was revealed that a pair of cyclopropene (a first tag) and tetrazine (a second tag) groups is the most efficient pair among the pairs examined. 4) On the basis of the results obtained in 3), this work synthesized azobenzene derivatives, which have a tetrazine in their terminal end.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (3 results)

All 2017 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] Pinpoint Chemical Modification of the Quinone-Access Channel of Mitochondrial Complex I via a Two-Step Conjugation Reaction2017

    • Author(s)
      Masuya Takahiro、Murai Masatoshi、Ito Takeshi、Aburaya Shunsuke、Aoki Wataru、Miyoshi Hideto
    • Journal Title

      Biochemistry

      Volume: 56 Issue: 32 Pages: 4279-4287

    • DOI

      10.1021/acs.biochem.7b00612

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] ミトコンドリア呼吸鎖複合体-Iの位置特異的化学修飾2017

    • Author(s)
      桝谷貴弘、村井正俊、三芳秀人
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2017 Annual Research Report
  • [Remarks] 京都大学農学研究科 生物機能制御化学分野ホームページ

    • URL

      http://www.biofunc-chem.kais.kyoto-u.ac.jp/

    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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