Development of new imaging methods to analyze the mechanism of cell differentiation in trees by direct visualization of single-cell

• Project/Area Number: 16K14954
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Wood science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Funada Ryo 東京農工大学, (連合)農学研究科(研究院), 教授 (20192734)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 木質バイオマス / 細胞分化 / 生体イメージング / 細胞壁 / 微小管 / 管状要素 / 培養細胞 / セルロース・ミクロフィブリル / 細胞壁構造 / 細胞骨格 / 植物ホルモン / 共焦点レーザ走査顕微鏡法 / アクチンフィラメント / 共焦点レーザ顕微鏡 / 生体イメージング解析

Outline of Final Research Achievements

In order to understand fully the mechanism of xylem differentiation in trees, new differentiation system that could induce formation of secondary xylem-like tracheary elements was developed from from calli of Populus sieboldi × P. grandidentata and Aesculus turbinate. Induced tracheary elements formed broad regions of thick cell walls, bordered pits and perforations that showed typical features of vessel elements and tracheids. Our new in vitro system allows the detailed analysis of the process of differentiation of secondary xylem. In addition, the orientation and localization of microtubules was visualized using stable expression of genes for microtubule-associated proteins with green fluorescent protein in a single differentiating tracheary element by real-time imaging techniques. The microtubules play an important role in the determination of pattern of cell wall structure.

Research Products

• [Int'l Joint Research] Universitas Gadjah Mada(インドネシア)
• [Int'l Joint Research] Bangladesh Agricultural University(バングラデシュ)
• [Int'l Joint Research] Nanyang Technological University(シンガポール)
• [Journal Article] Climate change and the regulation of wood formation in trees by temperature (2018)
  • Author(s): Begum, S., Kudo, K., Rahman, Md H., Nakaba, S., Yamagishi, Y., Nabeshima, E., Nugroho, D.W., Oribe, Y., Kitin, P., Jin H.O., Funada, R.
  • Journal Title: Trees-Structure and Function Volume: 32 Issue: 1 Pages: 3-15
  • DOI: 10.1007/s00468-017-1587-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Stem gravitropism and tension wood formation in Acacia mangium seedlings inclined at various angles (2018)
  • Author(s): Nugroho, W.D., Nakaba, S., Yamagishi, Y., Begum, S., Rahman, M.H., Kudo, K., Marsoem, S.N., Funada, R.
  • Journal Title: Annals of Botany Volume: 印刷中 Issue: 1 Pages: 87-94
  • DOI: 10.1093/aob/mcy056
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Formation of new networks of earlywood vessels in seedlings of the deciduous ring-porous hardwood Quercus serrata in springtime (2018)
  • Author(s): Kudo Kayo、Utsumi Yasuhiro、Kuroda Katsushi、Yamagishi Yusuke、Nabeshima Eri、Nakaba Satoshi、Yasue Koh、Takata Katsuhiko、Funada Ryo
  • Journal Title: Trees Volume: 印刷中 Issue: 3 Pages: 725-734
  • DOI: 10.1007/s00468-018-1667-2
  • Peer Reviewed
• [Journal Article] Responses of ray parenchyma cells to wounding differ between earlywood and latewood in the sapwood of Cryptomeria japonica (2017)
  • Author(s): Nakaba, S., Morimoto, H., Arakawa, I., Yamagishi, Y., Nakada, R., Funada, R.
  • Journal Title: Trees Volume: 31 Issue: 1 Pages: 31-39
  • DOI: 10.1007/s00468-016-1452-z
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Partial desiccation enhances induction of secondary xylem-like tracheary elements from calli of hybrid poplar (Populus sieboldii x P. grandidentata). (2016)
  • Author(s): Yusuke Yamagishi, Joto Yoshimoto, Suzuka Ide, Satoshi Nakaba, Eri Nabeshima, Ugai Watanabe, Ryo Funada
  • Journal Title: Trees-structure and function Volume: - Issue: 3 Pages: 1083-1089
  • DOI: 10.1007/s00468-016-1411-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Relationship between the earlywood-to-latewood transition and changes in levels of stored starch around the cambium in locally heated stems of the evergreen conifer Chamaecyparis pisifera (2016)
  • Author(s): Md Hasnat Rahman, Shahanara Begum, Satoshi Nakaba, Yusuke Yamagishi, Kayo Kudo, Eri Nabeshima, Widyanto Dwi Nugroho, Yuichiro Oribe, Ryo Funada
  • Journal Title: Trees－Structure and Function Volume: - Issue: 5 Pages: 1619-1630
  • DOI: 10.1007/s00468-016-1395-4
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 樹木木部細胞壁の形成機構 (2018)
  • Author(s): 船田 良, 山岸祐介, 半 智史
  • Organizer: セルロース学会北海道・東北支部セミナー
  • Invited
• [Presentation] 蛍光プローブによるポプラ培養細胞の管状要素誘導系におけるリグニン沈着の可視化 (2018)
  • Author(s): 吉田裕子，山岸祐介，飛松裕基，梅澤俊明，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] 心材形成過程の解析への自家蛍光を用いたスペクトルイメージングの応用 (2018)
  • Author(s): 半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] トチノキ培養細胞を用いたせん孔をもつ管状要素の分化誘導 (2018)
  • Author(s): 山岸祐介，鎌田 裕，荒川圭太，佐野雄三，工藤佳世，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] ゲッケイジュのカルス培養およびオイゲノールの分析に関する研究 (2018)
  • Author(s): 平木李奈，秋山佳貴，塚田健太郎，吉田裕子，Nuoendagula，梶田真也，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] カキノキにおける黒色心材様組織の誘導 (2018)
  • Author(s): 岩見佳奈，乃万 了，伴 琢也，船田 良，半 智史，松下泰幸
  • Organizer: 第68回日本木材学会大会
• [Presentation] Wood formation in tropical trees: understanding the changes in cambial activity in relation to the rainfall pattern in hardwood trees grown in Yogyakarta, Java Island, Indonesia (2018)
  • Author(s): MD Hasnat Rahman，Widyanto Dwi Nugroho，Satoshi Nakaba，Yusuke Yamagishi，Shahanara Begum，Sri Nugroho Marsoem，Ryo Funada
  • Organizer: 第68回日本木材学会大会
• [Presentation] オーキシンの移動の制御を目的とした剥皮およびNPA塗布処理による形成層細胞の変化 (2018)
  • Author(s): 吉永恵理子，Peter Kitin，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] チークの引張あて材における組織学的特徴の解析 (2018)
  • Author(s): 北見夏海斗，Widyanto Dwi Nugroho，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] オニグルミ樹幹におけるフェノール含有量の放射方向での変動 (2018)
  • Author(s): 宮田晴香，船田 良，半 智史，橋田 光，小林 真，吉田俊也，高橋直樹
  • Organizer: 第68回日本木材学会大会
• [Presentation] スギにおける周皮の分布と形成過程 (2018)
  • Author(s): 生越 恵，荒川 泉，船田 良，半 智史
  • Organizer: 第68回日本木材学会大会
• [Presentation] Effects of the application of exogenous auxin and defoliation on cambial reactivation and xylem differentiation in locally heated stems of Abies homolepis seedlings (2017)
  • Author(s): Md Hasnat Rahman, Shahanara Begum, Kayo Kudo, Takahiro Muraishi, Yusuke Yamagishi, Satoshi Nakaba, Ryo Funada
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] 交雑ポプラ培養細胞を用いたin vitro管状要素誘導に合成オーキシンNAAおよび2,4-Dが及ぼす影響 (2017)
  • Author(s): 山岸祐介、鎌田 裕、工藤佳世、半 智史、船田 良、荒川圭太、佐野雄三
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] 交雑ポプラ培養細胞の管状要素誘導系におけるリグニン沈着過程の解析 (2017)
  • Author(s): 吉田裕子、山岸祐介、半 智史、船田 良
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] Regulation of Cambial Activity in Trees: the Role of Temperature and Precipitation (2017)
  • Author(s): Ryo Funada, Md Hasnat Rahman, Shahanara Begum, Kayo Kudo, Yusuke Yamagishi, Eri Nabeshima, Widyanto Dwi Nugroho, Sri Nugroho Marsoem, Yuichiro Oribe, Satoshi Nakaba
  • Organizer: The 9th Pacific Regional Wood Anatomy Conference
  • Int'l Joint Research
• [Presentation] Tracheary Elements in Calli of Japanese Horse Chestnut (Aesculus turbinata) Form Perforation Plates Like Structures (2017)
  • Author(s): Yusuke Yamagishi, Yutaka Kamada, Kayo Kudo, Satoshi Nakaba, Yuzou Sano, Ryo Funada
  • Organizer: The 9th Pacific Regional Wood Anatomy Conference
  • Int'l Joint Research
• [Presentation] The Pattern of Location of the First Earlywood Vessels in the Current year’s xylem in a Ring-porous Hardwood, Quercus serrata (2017)
  • Author(s): Kayo Kudo, Yuichiro Oribe, Shahanara Begum, Yusuke Yamagishi, Eri Nabeshima, Md Hasnat Rahman, Satoshi Nakaba, Katsuhiko Takata, Ryo Funada
  • Organizer: The 9th Pacific Regional Wood Anatomy Conference
  • Int'l Joint Research
• [Presentation] 交雑ポプラカルスにおける二次木部様管状要素の分化 (2016)
  • Author(s): 山岸祐介、吉田裕子、渡辺宇外、半 智史、船田 良、荒川圭太、佐野雄三
  • Organizer: 第34回日本植物細胞分子生物学会大会
  • Place of Presentation: 信州大学（長野県上田市）
  • Year and Date: 2016-09-01
• [Presentation] Regulation of wood formation in trees: the role of temperature in cambial activity (2016)
  • Author(s): Ryo Funada, Shahanara Begum, Kayo Kudo, Md Hasnat Rahman, Yusuke Yamagishi, Eri Nabeshima, Widyanto Dwi Nugroho, Yuichiro Oribe, Satoshi Nakaba
  • Organizer: International Academy of Wood Science Meeting
  • Place of Presentation: Academie d’Agriculture de France (Paris, France)
  • Year and Date: 2016-06-01
  • Int'l Joint Research
• [Remarks] 東京農工大学植物資源形成学研究室
  • URL: http://web.tuat.ac.jp/~keisei/
• [Remarks] 東京農工大学研究者情報
  • URL: http://kenkyu-web.tuat.ac.jp/Profiles/1/0000023/profile.html