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Methodological trial of the xenotransplantation of pig embryonic kidney to cats

Research Project

Project/Area Number 16K15045
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionThe University of Tokyo

Principal Investigator

Yonezawa Tomohiro  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (10433715)

Co-Investigator(Kenkyū-buntansha) 長嶋 比呂志  明治大学, 農学部, 専任教授 (50318664)
横尾 隆  東京慈恵会医科大学, 医学部, 教授 (70301538)
Research Collaborator Iwai Satomi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords再生医療 / 腎臓病 / 内科 / 獣医学 / 異種間移植 / ネコ / 腎臓 / 再生医学 / 生理学
Outline of Final Research Achievements

No good therapy for the kidney disease has been established. Kidney transplantation is one of the treatments, but it is too difficult to perform it because it is too hard to find the donor. Therefore, we have focused on xenotransplanted embryonic kidney which provides a niche for endogenous mesenchymal stem cell differentiation into erythropoietin-producing tissue. In this study, we investigated the possibility of xenotransplantation of the pig fatal kidney to the cat. First, we have established the ELISA method to assess the immune intensity against the pig antigens in the recipient. Second, we made the protocols for operation of xenotransplantation using the fundamental data. Third, we have investigated the immune-suppressing method to the donor during operation. These findings lead to establishment of the new therapy for the kidney disease.

Academic Significance and Societal Importance of the Research Achievements

本研究は胎生組織ニッチ法の成功例の一つとして、再生医療に革新的な変化をもたらす可能性がある。また、胎生組織ニッチ法には胎子が必要であるが、ネコの胎子を安定して入手するのは困難を極めるのに対し、ブタの胎子後腎を用いることでドナー確保の困難を解決することができる可能性がある。ブタはこれまでも異種間移植の研究に用いられてきた経緯があり、拒絶反応を防ぐノウハウについて多くの先行研究があること、実験動物であるため遺伝子組み換え技術が確立していることなど、多くの利点がある。さらに、本応募の再生組織から産生されるエリスロポエチンは自己由来であるため、腎性貧血の治療にも役立つことが期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report

Research Products

(20 results)

All 2019 2018 2017 2016

All Journal Article (17 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 17 results,  Open Access: 10 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Int'l Joint Research: 2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Assessment of HER2 Expression in Canine Urothelial Carcinoma of the Urinary Bladder2019

    • Author(s)
      Tsuboi Masaya、Sakai Kosei、Maeda Shingo、Chambers James K.、Yonezawa Tomohiro、Matsuki Naoaki、Uchida Kazuyuki、Nakayama Hiroyuki
    • Journal Title

      Veterinary Pathology

      Volume: 56 Pages: 369-376

    • DOI

      10.1177/0300985818817024

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Excitatory and Inhibitory Amino Acids in Cerebrospinal Fluid of Familial Spontaneous Epileptic Cats2019

    • Author(s)
      Fukie Ogawa, Daisuke Hasegawa, Shunta Mizoguchi, Tomohiro Yonezawa, Naoaki Matsuki
    • Journal Title

      Journal of Veterinary Science & Medical Diagnosis

      Volume: 8:1 Pages: 1-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anti-tumour effect of lapatinib in canine transitional cell carcinoma cell lines2018

    • Author(s)
      Sakai Kosei、Maeda Shingo、Saeki Kohei、Nakagawa Takayuki、Murakami Mami、Endo Yoshifumi、Yonezawa Tomohiro、Kadosawa Tsuyoshi、Mori Takashi、Nishimura Ryohei、Matsuki Naoaki
    • Journal Title

      Veterinary and Comparative Oncology

      Volume: 16 Pages: 642-649

    • DOI

      10.1111/vco.12434

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive gene expression analysis of canine invasive urothelial bladder carcinoma by RNA-Seq2018

    • Author(s)
      Maeda Shingo、Tomiyasu Hirotaka、Tsuboi Masaya、Inoue Akiko、Ishihara Genki、Uchikai Takao、Chambers James K.、Uchida Kazuyuki、Yonezawa Tomohiro、Matsuki Naoaki
    • Journal Title

      BMC Cancer

      Volume: 18 Pages: 472-472

    • DOI

      10.1186/s12885-018-4409-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multiple acquired portosystemic shunts secondary to primary hypoplasia of the portal vein in a cat2018

    • Author(s)
      SUGIMOTO Satoko、MAEDA Shingo、TSUBOI Masaya、SAEKI Kohei、CHAMBERS James K.、YONEZAWA Tomohiro、FUKUSHIMA Kenjiro、FUJIWARA Reina、UCHIDA Kazuyuki、TSUJIMOTO Hajime、MATSUKI Naoaki、OHNO Koichi
    • Journal Title

      J. Vet. Med. Sci.

      Volume: 80 Issue: 6 Pages: 874-877

    • DOI

      10.1292/jvms.17-0648

    • NAID

      130007384664

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of tumour-infiltrating regulatory T cells with adverse outcomes in dogs with malignant tumours2018

    • Author(s)
      Sakai K., Maeda S., Yamada Y., Chambers J. K., Uchida K., Nakayama H., Yonezawa T., Matsuki N.
    • Journal Title

      Veterinary and Comparative Oncology

      Volume: 印刷中 Pages: 330-336

    • DOI

      10.1111/vco.12383

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Clinical Usefulness of Flash Glucose Monitoring System in Dogs with Diabetes Mellitus2018

    • Author(s)
      Takashi Tamamoto, Mizuki Kumano, Hirotaka Igarashi, Tomohiro Yonezawa
    • Journal Title

      Journal of Veterinary Science & Medical Diagnosis

      Volume: 7:5 Pages: 1-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Presence of contagious yawning in sheep2018

    • Author(s)
      Yonezawa Tomohiro、Sato Kohei、Uchida Mona、Matsuki Naoaki、Yamazaki Atusi
    • Journal Title

      Anim Sci J.

      Volume: 88 Pages: 195-200

    • DOI

      10.1111/asj.12681

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Decreased plasma amino acid concentrations in cats with chronic gastrointestinal diseases and their possible contribution in the inflammatory response.2017

    • Author(s)
      Sakai K, Maeda S, Yonezawa T, Matsuki N.
    • Journal Title

      Vet Immunol Immunopathol

      Volume: 2018 Pages: 1-6

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fecal microbiome in dogs with inflammatory bowel disease and intestinal lymphoma2017

    • Author(s)
      Omori M, Maeda S, Igarashi H, Ohno K, Sakai K, Yonezawa T, Horigome A, Odamaki T, Matsuki N
    • Journal Title

      J. Vet. Med. Sci.

      Volume: 79(11) Issue: 11 Pages: 1840-1847

    • DOI

      10.1292/jvms.17-0045

    • NAID

      130006206562

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Density of tumor-infiltrating granzyme B-positive cells predicts favorable prognosis in dogs with transitional cell carcinoma2017

    • Author(s)
      Inoue Akiko、Maeda Shingo、Kinoshita Ryohei、Tsuboi Masaya、Yonezawa Tomohiro、Matsuki Naoaki
    • Journal Title

      Veterinary Immunology Immunopathology

      Volume: 190 Pages: 53-56

    • DOI

      10.1016/j.vetimm.2017.07.001

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Endoscopic Cytology for the Diagnosis of Chronic Enteritis and Intestinal Lymphoma in Dogs2017

    • Author(s)
      Maeda S.、Tsuboi M.、Sakai K.、Ohno K.、Fukushima K.、Kanemoto H.、Hiyoshi-Kanemoto S.、Goto-Koshino Y.、Chambers J. K.、Yonezawa T.、Uchida K.、Matsuki N.
    • Journal Title

      Vet Pathol.

      Volume: 54 Pages: 595-604

    • DOI

      10.1177/0300985817705175

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cycle Influences Spontaneous Delivery in Cows.2016

    • Author(s)
      Yonezawa T, Uchida M, Tomioka M, Matsuki N
    • Journal Title

      PLoS One

      Volume: 11(8)

    • DOI

      10.1371/journal.pone.0161735

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Apoptosis inhibitor of macrophage (AIM) reduces cell number in canine histiocytic sarcoma cell lines2016

    • Author(s)
      Uchida M, Saeki K, Maeda S, Tamahara S, Yonezawa T, Matsuki N
    • Journal Title

      J. Vet. Med. Sci.

      Volume: 78(9) Issue: 9 Pages: 1515-1520

    • DOI

      10.1292/jvms.16-0120

    • NAID

      130005598036

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Prolactin suppression of Gonadotropin releasing hormone initiation of mammary gland involution in female rats.2016

    • Author(s)
      Rieanrakwong D, Laoharatchatathanin T, Terashima R, Yonezawa T, Kurusu S, Hasegawa Y, Kawaminami M.
    • Journal Title

      Endocrinology

      Volume: 157(7) Pages: 2750-2758

    • DOI

      10.1210/en.2016-1180

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders2016

    • Author(s)
      Murakami K, Maeda S, Yonezawa T, Matsuki N
    • Journal Title

      J. Vet. Med. Sci.

      Volume: 78(6) Issue: 6 Pages: 1051-1054

    • DOI

      10.1292/jvms.15-0711

    • NAID

      130005159665

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synovial fluid total protein concentration as a possible marker for canine idiopathic polyarthritis2016

    • Author(s)
      Murakami K, Yonezawa T, Matsuki N.
    • Journal Title

      J. Vet. Med. Sci.

      Volume: 77(12) Issue: 12 Pages: 1715-1717

    • DOI

      10.1292/jvms.15-0263

    • NAID

      130005115336

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Syngeneic ANXA5 inhibition of mammary tumor cell growth in the dog2017

    • Author(s)
      Mona Uchida, Kohei Saeki, Shingo Maeda, Tomohiro Yonezawa, Naoaki Matsuki and Mitsumori Kawaminami
    • Organizer
      Annexin Conference 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Relationship between lunar cycle and spontaneous delivery date in Holstein cows2017

    • Author(s)
      Tomohiro Yonezawa, Dai Ishiyama, Mona Uchida, Michiko Tomioka, Kazunori Ishii, Shizuko Maeda, Yoji Sasai, Masatsugu Asada, Tadashi Kawamura, Yoshihiro Nakamura, Eimei Sato, Shingo Maeda, Naoaki Matsuki
    • Organizer
      WCRB2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 腫瘍の治療剤およびその使用2016

    • Inventor(s)
      米澤智洋、内田萌菜、松木直章、金村優香、川上和夫
    • Industrial Property Rights Holder
      米澤智洋、内田萌菜、松木直章、金村優香、川上和夫
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-05-26
    • Related Report
      2016 Research-status Report
    • Overseas

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Published: 2016-04-21   Modified: 2020-03-30  

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