Analysis of the bone metabolism failure in the aging animals and establishment of the preventive maintenance plan based on the animal welfare

• Project/Area Number: 16K15057
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: The University of Tokyo
• Principal Investigator: Endo Hideki 東京大学, 総合研究博物館, 教授 (30249908)
• Co-Investigator(Kenkyū-buntansha): 村田 浩一 日本大学, 生物資源科学部, 教授 (00339285) ; 鯉江 洋 日本大学, 生物資源科学部, 教授 (20267040) ; 中山 裕之 東京大学, 大学院農学生命科学研究科(農学部), 教授 (40155891)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 高齢 / 病理 / 飼育動物 / 動物園 / 骨増生 / 骨変形 / 動物福祉 / QOL / 超高齢動物 / 病理学 / 骨格 / 水族館 / 野生動物 / 骨形質 / 機能形態学 / 骨病理学 / 飼育技術

Outline of Final Research Achievements

Skeletons of aging animals were collected, macro morphological changes of bones were investigated, and three dimensional images were successfully reconstructed. In Asian elephant, hippopotamus, white rhinoceros and giraffe, macro morphological anomalies in the spine, limbs, skull were detected and a fundamental theory of bone aging was constructed. In old Asian elephants, we discussed the problems of the jaw and molar tooth using three dimensional images. In medium-sized animals we observed abnormalities in the jaw and facial function, and also in birds and reptiles, we could establish the theory on the aging and morphological changes along with the physiological background. The results succeeded in greatly expanding the opportunities for collecting basic biological and pathological data on maintained animals, in addition to the direct research of aging animals. Based on these results, we also could develop the new opinion of the animal welfare towards the zoos and aquaria.

Research Products

• [Int'l Joint Research] カセサート大学/タイ国立公園局・天然資源環境省(タイ)
• [Int'l Joint Research] ベトナム生態生物資源研究所(ベトナム)
• [Journal Article] Geographical variation in the skeletal morphology of Red Jungle Fowl (2017)
  • Author(s): H. Endo, N. Tsunekawa, M. Sonoe, T. Sasaki, H. Ogawa, T. Amano, N. T. Son, V. Phimphachanhvongsod, K. Kudo, T. Yonezawa & F. Akishinonomiya
  • Journal Title: British Poultry Science Volume: 印刷中 Issue: 4 Pages: 348-357
  • DOI: 10.1080/00071668.2017.1311008
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Morphological variation in brain through domestication of fowl (2017)
  • Author(s): Kawabe Soichiro、Tsunekawa Naoki、Kudo Kohei、Tirawattanawanich Chanin、Akishinonomiya Fumihito、Endo Hideki
  • Journal Title: Journal of Anatomy Volume: 231 Issue: 2 Pages: 287-297
  • DOI: 10.1111/joa.12623
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Macroscopic and CT examinations of the mastication mechanism in the southern tamandua. (2017)
  • Author(s): Endo, H., Ito, K., Watabe, H., Son, N.T., Koyabu, D.
  • Journal Title: Mammal Study Volume: 42 Issue: 2 Pages: 89-96
  • DOI: 10.3106/041.042.0203
  • NAID: 40021221598
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Into the dark: patterns of middle ear adaptations in subterranean eulipotyphlan mammals (2017)
  • Author(s): Koyabu Daisuke、Hosojima Misato、Endo Hideki
  • Journal Title: Royal Society Open Science Volume: 4 Issue: 9 Pages: 170608-170608
  • DOI: 10.1098/rsos.170608
  • Peer Reviewed / Open Access
• [Journal Article] Growth Pattern and Functional Morphology of the Cervical Vertebrae in the Gerenuk (Litocranius walleri): The Evolution of Neck Elongation in Antilopini (Bovidae, Artiodactyla) (2017)
  • Author(s): Gunji Megu、Endo Hideki
  • Journal Title: Journal of Mammalian Evolution Volume: - Issue: 2 Pages: 225-235
  • DOI: 10.1007/s10914-017-9396-7
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of reference values for complete blood count and blood gases in cynomolgus monkeys (<i>Macaca fascicularis</i>) (2017)
  • Author(s): hunya Nakayama, Hiroshi Koie, Kiichi Kanayama, Yuko Katakai, Yasuyo Ito-Fujishiro, Tadashi Sankai, Yasuhiro Yasutomi, Naohide Ageyama
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 5 Pages: 881-888
  • DOI: 10.1292/jvms.16-0638
  • NAID: 130007331475
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Adenocarcinoma of Barrett’s esophagus in a dog (2017)
  • Author(s): James K. Chambers, Saito Tsubasa, Fukushima Kenjiro, Kakuta Shigeru, Nakayama Jun, Ohno Koichi, Tsujimoto Hajime, Nakayama Hiroyuki, Uchida Kazuyuki
  • Journal Title: Journal of Toxicologic Pathology Volume: 30 Issue: 3 Pages: 239-243
  • DOI: 10.1293/tox.2017-0009
  • NAID: 130005815283
  • ISSN: 0914-9198, 1881-915X
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Variations in Histiocytic Differentiation of Cell Lines From Canine Cerebral and Articular Histiocytic Sarcomas. (2017)
  • Author(s): Thongtharb A, Uchida K, Chambers JK, Nakayama H.
  • Journal Title: Veterinary Pathology Volume: In press Issue: 3 Pages: 395-404
  • DOI: 10.1177/0300985817690211
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immunohistochemical analysis of canine and feline muscle disorders using formalin-fixed, paraffin-embedded tissues. (2017)
  • Author(s): Shiga T, Uchida K, Chambers JK, Nakayama H.
  • Journal Title: J Vet Diagn Invest. Volume: 29(6): Issue: 6 Pages: 805-813
  • DOI: 10.1177/1040638717715287
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Histopathological and Immunohistochemical Study of Trichoblastoma in the Rabbit (2017)
  • Author(s): Kok M.K.、Chambers J.K.、Ushio N.、Watamori A.、Miwa Y.、Nakayama H.、Uchida K.
  • Journal Title: Journal of Comparative Pathology Volume: 157 Issue: 2-3 Pages: 126-135
  • DOI: 10.1016/j.jcpa.2017.06.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] IgA Antibodies Against Gliadin and Tissue Transglutaminase in Dogs With Chronic Enteritis and Intestinal T-Cell Lymphoma (2017)
  • Author(s): Matsumoto, I., Uchida, K., Nakashima, K., Hiyoshi, S., Chambers, J.K., Tsujimoto, H. and Nakayama, H.
  • Journal Title: Veterinary Pathology Volume: 55 Issue: 1 Pages: 98-107
  • DOI: 10.1177/0300985817690212
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isolation of <i>Streptococcus gallolyticus</i> with very high degradability of condensed tannins from feces of the wild Japanese rock ptarmigans on Mt. Tateyama (2017)
  • Author(s): Tsuchida Sayaka, Murata Koichi, Ohkuma Moriya, Ushida Kazunari
  • Journal Title: The Journal of General and Applied Microbiology Volume: 63 Issue: 3 Pages: 195-198
  • DOI: 10.2323/jgam.2016.09.003
  • NAID: 130005745311
  • ISSN: 0022-1260, 1349-8037
  • Peer Reviewed / Open Access
• [Journal Article] Prevalence of avian haemosporidia among injured wild birds in Tokyo and environs, Japan (2017)
  • Author(s): Inumaru Mizue、Murata Koichi、Sato Yukita
  • Journal Title: International Journal for Parasitology Volume: 6 Issue: 3 Pages: 299-309
  • DOI: 10.1016/j.ijppaw.2017.09.007
  • Peer Reviewed / Open Access
• [Journal Article] Comparative Functional Morphology of the Skeletal Forelimb, Pectoral Girdle, and Sternum in Japanese Native Domestic Fowls (2017)
  • Author(s): Kudo, K., Tsunekawa, N., Ogawa, H. and Endo, H.
  • Journal Title: The Journal of Poultry Science Volume: 54 Issue: 1 Pages: 47-57
  • DOI: 10.2141/jpsa.0160060
  • NAID: 130005300420
  • ISSN: 1346-7395, 1349-0486
  • Peer Reviewed / Open Access
• [Journal Article] Musculoskeletal System of Huge Tarsometatarsal Region in the Dong Tao Fowls from North Vietnam (2017)
  • Author(s): Endo, H., Tsunekawa, N., Kudo, K., Hayashi, Y., Ikeya, K., Nguyen, T. S. and Akishinonomiya, F.
  • Journal Title: The Journal of Poultry Science Volume: 54 Issue: 1 Pages: 58-65
  • DOI: 10.2141/jpsa.0160074
  • NAID: 130005300335
  • ISSN: 1346-7395, 1349-0486
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phylogenomics and morphology of extinct paleognaths reveal the origin and evolution of the ratites (2017)
  • Author(s): Takahiro Yonezawa, Takahiro Segawa, Hiroshi Mori, Paula F. Campos, Yuichi Hongoh et al.,
  • Journal Title: Current Biology Volume: 27 Issue: 1 Pages: 68-77
  • DOI: 10.1016/j.cub.2016.10.029
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mobility of the forearm in the raccoon (<i>Procyon lotor</i>), raccoon dog (<i>Nyctereutes procyonoides</i>) and red panda (<i>Ailurus fulgens</i>) (2017)
  • Author(s): Kamioka, M., Sasaki, M., Yamada, K., Endo, H., Oishi, M., Yuhara, K., Tomikawa, S., Sugimoto, M., Oshida, T., Kondoh, D. and Kitamura, N.
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 1 Pages: 224-229
  • DOI: 10.1292/jvms.16-0241
  • NAID: 130005296372
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Functional cervicothoracic boundary modified by anatomical shifts in the neck of giraffe. (2016)
  • Author(s): Gunji, M. and Endo, H.
  • Journal Title: Royal Society Open Science Volume: 3 Issue: 2 Pages: 150604-150604
  • DOI: 10.1098/rsos.150604
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Bats (Mammalia: Chiroptera) of the southeastern Truong Son Mountain, Quang Ngai province, Vietnam. (2016)
  • Author(s): Nguyen, T. S., O’shea, T. J., Gore, J. A., Csorba, G., Vuong, T. T., Oshida, T., Endo, H. and Motokawa, M.
  • Journal Title: Journal of Threatened Taxa Volume: 8 Pages: 8953-8969
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Compartive study of physiological cross-sectional area of masticatory muscles among species of Carnivora. (2016)
  • Author(s): Ito, K. and Endo, H.
  • Journal Title: Mammal Study Volume: 41 Issue: 4 Pages: 181-190
  • DOI: 10.3106/041.041.0403
  • NAID: 40021059506
  • Peer Reviewed / Open Access
• [Journal Article] Migratory ability of gonadal germ cells (GGCs) isolated from <i>Ciconia boyciana</i> and <i>Geronticus eremita</i> embryos into the gonad of developing chicken embryos (2016)
  • Author(s): Nakajima Y, Fukuda H, Onuma M, Murata K, Ueda M, Sunaga E, Shiraishi T, Tajima A.
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 6 Pages: 1055-1058
  • DOI: 10.1292/jvms.15-0664
  • NAID: 130005159661
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Cellular conservation of endangered midget buffalo (Lowland Anoa, Bubalus quarlesi) by establishment of primary cultured cell, and its immortalization with expression of cell cycle regulators. (2016)
  • Author(s): Fukuda T, Iino Y, Eitsuka T, Onuma M, Katayama M, Murata K, Inoue-Murayama M, Hara K, Isogai E, Kiyono T
  • Journal Title: Cytotechnology Volume: 68 Issue: 5 Pages: 1937-1947
  • DOI: 10.1007/s10616-016-0004-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Exaptation of Bornavirus-Like Nucleoprotein Elements in Afrotherians. (2016)
  • Author(s): Kobayashi Y, Horie M, Nakano A, Murata K, Itou T, Suzuki Y.
  • Journal Title: PLoS Pathog. Volume: 12 Issue: 8 Pages: e1005785-e1005785
  • DOI: 10.1371/journal.ppat.1005785
  • Peer Reviewed / Open Access
• [Journal Article] Diversity of MHC class I alleles in Spheniscus humboldti. (2016)
  • Author(s): Kikkawa, E., Tanaka, M., Naruse, T. K., Tsuda, T. T., Tsuda, M., Murata, K. and Kimura, A.
  • Journal Title: Immunogenetics Volume: 69 Issue: 2 Pages: 113-124
  • DOI: 10.1007/s00251-016-0951-9
  • Peer Reviewed
• [Journal Article] Pathology of the aging brain in domestic and laboratory animals, and animal models of humen neurodegenerative diseases. (2016)
  • Author(s): Youssef, S. A., Capucchio, M. T., Rofina, J. E., Chambers, J. K., Uchida, K., Nakayama, H. and Head, E.
  • Journal Title: Veterinary Pathology Volume: 53 Issue: 2 Pages: 327-348
  • DOI: 10.1177/0300985815623997
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tracking cells implanted into cynomolgus monkeys (<i>Macaca fascicularis</i>) using MRI (2016)
  • Author(s): Fujishiro Y, Koie H, Shibata H, Okabayashi S, Katakai Y, Ohno C, Kanayama K, Yasutomi Y, Ageyama N.
  • Journal Title: Experimental Animals Volume: 65 Issue: 3 Pages: 311-318
  • DOI: 10.1538/expanim.15-0125
  • NAID: 130006882369
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Analysis of the breast milk of giant pandas (<i>Ailuropoda melanoleuca</i>) and the preparation of substitutes (2016)
  • Author(s): Zhang, Z., Hou, R., Lan, J., Wang, H., Kurokawa, H., Takatsu, Z., Kobayashi, T., Koie, H., Kamata, H., Kanayama, K. and Watanabe, T.
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 5 Pages: 747-754
  • DOI: 10.1292/jvms.15-0677
  • NAID: 130005154289
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 伴侶のコントラスト (2018)
  • Author(s): 遠藤秀紀
  • Organizer: 日本美術解剖学会2018年大会
  • Invited
• [Presentation] 頭骨形態を指標とした束柱類（哺乳類: アフリカ獣類）の水頭骨形態を指標と した束柱類（哺乳類：アフリカ獣類）の水棲適応の定量的解析―Paleoparadoxia 梁川標本を例に― (2017)
  • Author(s): 松井久美子・河部壮一郎・遠藤秀紀・對比地孝亘・甲能直樹
  • Organizer: 日本古生物学会
• [Presentation] Comparative three-dimensional CT image analysis of the mastication system in the giant anteater and southern tamandua (2017)
  • Author(s): Hideki Endo, Kohei Kudo, Junpei Kimura
  • Organizer: 12 th International Mammalogical Congress
  • Int'l Joint Research
• [Presentation] 動物園内外の情勢変化と研究の未来 (2017)
  • Author(s): 遠藤秀紀
  • Organizer: 第23回日本野生動物医学会大会
  • Invited
• [Presentation] 台湾左鎮から産出した大型ワニ類の分類学的再検討 (2017)
  • Author(s): 武川 愛, 青木良輔, 平山 廉, 吉田将崇, 昆 彭生, 遠藤秀紀
  • Organizer: 日本古生物学会
  • Place of Presentation: 早稲田大学（東京都新宿区）
• [Presentation] 食肉類における咀嚼筋の比較機能形態学的検討 (2016)
  • Author(s): 伊藤 海, 遠藤秀紀
  • Organizer: 日本哺乳類学会2016年度大会
  • Place of Presentation: 筑波大学（茨城県つくば市）
  • Year and Date: 2016-09-23
• [Presentation] ゲレヌク（Litocranius walleri）における頸椎の形態と成長様式 (2016)
  • Author(s): 郡司芽久, 遠藤秀紀
  • Organizer: 日本哺乳類学会2016年度大会
  • Place of Presentation: 筑波大学（茨城県つくば市）
  • Year and Date: 2016-09-23
• [Presentation] 日本鶏における後肢、後肢帯、腰仙骨の機能形態学的特徴と育種動機との関係について (2016)
  • Author(s): 工藤光平, 遠藤秀紀
  • Organizer: 日本家禽学会第120回大会
  • Place of Presentation: 静岡県総合研修所もくせい会館（静岡県静岡市）
• [Book] 有袋類学 (2018)
  • Author(s): 遠藤 秀紀
  • Total Pages: 258
  • Publisher: 東京大学出版会
  • ISBN: 9784130602549
• [Book] 東京大学総合研究博物館所蔵 鶏骨格標本および剥製標本の基礎資料集 (2016)
  • Author(s): 工藤光平, 遠藤秀紀
  • Total Pages: 254
  • Publisher: 東京大学総合研究博物館
• [Remarks] Laboratory of Dead Body Science
  • URL: http://www.um.u-tokyo.ac.jp/people/faculty_endo.html
• [Remarks] 東京大学総合研究博物館 遺体科学研究室
  • URL: http://www.um.u-tokyo.ac.jp/endo/
• [Remarks] 日本大学生物資源科学部動物資源科学科 野生動物学研究室
  • URL: http://hp.brs.nihon-u.ac.jp/~wildlife/
• [Remarks] 東京大学大学院農学生命科学研究科 獣医病理学研究室
  • URL: http://www.vm.a.u-tokyo.ac.jp/byouri/
• [Remarks] 日本大学生物資源科学部獣医学科獣医生理学研究室
  • URL: http://hp.brs.nihon-u.ac.jp/~vetphys/