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Identification of an ER-Golgi cargo receptor for boric acid channels in plant cells

Research Project

Project/Area Number 16K15082
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionOsaka Prefecture University

Principal Investigator

Junpei Takano  大阪府立大学, 生命環境科学研究科, 教授 (70532472)

Research Collaborator Nakamura Shunske  北海道大学, 大学院
Yamasaki Arisa  大阪府立大学, 大学院
Zhang Zhe  大阪府立大学, 大学院
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsカーゴレセプター / ホウ酸チャネル / シロイヌナズナ / 小胞体 / 分泌系 / ゴルジ体 / 花粉 / 小胞輸送 / ER exit / ホウ酸 / チャネル / 植物 / 生理学 / 蛋白質
Outline of Final Research Achievements

In this study, we suggested that SPOT1/KNS3 functions as a cargo receptor for boric acid channels from the endoplasmic reticulum (ER) to the plasma membrane in plant cells. KNS3 and homologs probably bind with boric acid channels on the ER membrane to selectively transport into transport vesicles toward the Golgi. In the course of this study, we also revealed the localization of a boric acid channel NIP7;1 in the plasma membrane of anther tapetum cells although the localization was not dependent on KNS3.

Academic Significance and Societal Importance of the Research Achievements

膜タンパク質は小胞体で合成され、ゴルジ体を経由して機能する膜へ輸送される。最初の輸送ステップは小胞体における輸送小胞への積み込みであるが、その仕組みは単純ではないことが様々な生物で明らかになりつつある。本研究は植物のホウ酸チャネルという特定の膜タンパク質を選択的に輸送小胞に積み込む新たな仕組みを見出したものである。これは細胞膜で働くホウ酸チャネルの量を正確に調整する植物にユニークな仕組みと考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (2 results) Remarks (2 results)

  • [Int'l Joint Research] University of Tennessee(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] テネシー大学(米国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Nodulin Intrinsic Protein 7;1 is a tapetal boric acid channel involved in pollen cell wall formation.2018

    • Author(s)
      Routray P, Li T, Yamasaki A, Yoshinari A, Takano J, Choi WG, Sams CE, and Roberts DM
    • Journal Title

      Plant Physiology

      Volume: 178 Issue: 3 Pages: 1269-1269

    • DOI

      10.1104/pp.18.00604

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] シロイヌナズナにおけるホウ酸チャネル特異的な ER-ゴルジカーゴレセプター候補タンパク質 SPOT1/KNS3 の解析2018

    • Author(s)
      山崎有紗・中村俊介・石黒澄衛・高野順平
    • Organizer
      土壌肥料学会 日大藤沢キャンパス
    • Related Report
      2018 Annual Research Report
  • [Presentation] SPOT1/KNS3はホウ酸チャネルのER exitに重要である2017

    • Author(s)
      中村俊介, 竹村 駿介,石黒 澄衛,内藤 哲,高野 順平
    • Organizer
      日本植物生理学会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Remarks] 植物栽培生理学研究室

    • URL

      https://saibaiseirigaku.wixsite.com/crop-ecophysiology

    • Related Report
      2018 Annual Research Report
  • [Remarks] 植物栽培生理学研究グループ

    • URL

      http://saibaiseirigaku.wixsite.com/crop-ecophysiology

    • Related Report
      2017 Research-status Report 2016 Research-status Report

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Published: 2016-04-21   Modified: 2024-12-25  

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