Molecular mechanism of IL-17 production in anti-fungal immunity

• Project/Area Number: 16K15271
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Chiba University
• Principal Investigator: SAIJO Shinobu 千葉大学, 真菌医学研究センター, 准教授 (60396877)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 感染免疫 / サイトカイン / 真菌感染 / 真菌 / 感染症 / 免疫 / 遺伝子改変マウス / 細菌

Outline of Final Research Achievements

Fungi are ubiquitous in environments and host immune systems are constantly engaged with these eukaryotic pathogens. We have reported taht C-type lectin receptors (CLRs) that expressed in myeloid cells play a central role in host defenses against fungal infections by coordinating immune systems. Cytokine production is one of the most characteristic and crucial responses in anti-fungal immunity led by ligand ligation of CLRs. However, in vivo mechanisms of this process has not been fully explained . In this study, we describe the molecular mechanisms of cytokine production in anti-fungal activities by the host immune systems.

Research Products

• [Journal Article] C-type lectin receptors in anti-fungal immunity. (2017)
  • Author(s): Shiokawa M., Yamasaki S., Saijo S.,
  • Journal Title: Current Opinion in Microbiology Volume: 40 Pages: 123-130
  • DOI: 10.1016/j.mib.2017.11.004
  • Peer Reviewed / Open Access
• [Journal Article] LC3-associated phagocytosis is required for dendritic cell inflammatory cytokine response to gut commensal yeast Saccharomyces cerevisiae. (2017)
  • Author(s): Lamprinaki, D., Beasy, G., Zhekova, A., Wittmann, A., James, S., Dicks, J., Iwakura, Y., Saijo, S., Wang, X., Chow, C. W., Roberts, I., Korcsmaros, T., Mayer, U., Wileman, T., and Kawasaki, N.
  • Journal Title: Front Immunol. Volume: 8 Pages: 1397-1397
  • DOI: 10.3389/fimmu.2017.01397
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dectin-1 Plays an Important Role in House Dust Mite-Induced Allergic Airway Inflammation through the Activation of CD11b+ Dendritic Cells. (2017)
  • Author(s): Ito T, Hirose K, Norimoto A, Tamachi T, Yokota M, Saku A, Takatori H, Saijo S, Iwakura Y, Nakajima H.
  • Journal Title: J Immunol Volume: 198 Pages: 61-70
  • DOI: 10.4049/jimmunol.1502393
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A critical role of Dectin-1 in hypersensitivity pneumonitis (2016)
  • Author(s): Higashino-Kameda M, Yabe-Wada T, Matsuba S, Takeda K, Anzawa K, Mochizuki T, Makimura K, Saijo S, Iwakura Y, Toga H, Nakamura A
  • Journal Title: Inflamm Res. Volume: 65 Pages: 235-244
  • DOI: 10.1007/s00011-015-0910-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dectin-2-dependent host defense in mice infected with serotype 3 Streptococcus pneumoniae (2016)
  • Author(s): Akahori Y, Miyasaka T, Toyama M, Matsumoto I, Miyahara A, Zong T, Ishii K, Kinjo Y, Miyazaki Y, Saijo S, Iwakura Y, Kawakami K.
  • Journal Title: BMC Immunol. Volume: 17 Pages: 1-1
  • DOI: 10.1186/s12865-015-0139-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dectin-1 and Dectin-2 promote control of the fungal pathogen Trichophyton rubrum independently of IL-17 and adaptive immunity in experimental deep dermatophytosis (2016)
  • Author(s): Fabio Yoshikawa, Rikio Yabe, Yoichiro Iwakura, Sandro Rogerio de Almeida, Shinobu Saijo
  • Journal Title: Innate Immun. Volume: in press Pages: 316-24
  • DOI: 10.1177/1753425916645392
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phosphoinositide 3-Kinase δ Regulates Dectin-2 Signaling and the Generation of Th2 and Th17 Immunity. (2016)
  • Author(s): Lee MJ, Yoshimoto E, Saijo S, Iwakura Y, Lin X, Katz HR, Kanaoka Y, Barrett NA.
  • Journal Title: J Immunol. Volume: 197 Pages: 278-287
  • DOI: 10.4049/jimmunol.1502485
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dectin-2 Recognizes Mannosylated O-antigens of Human Opportunistic Pathogens and Augments Lipopolysaccharide Activation of Myeloid Cells. (2016)
  • Author(s): Wittmann A, Lamprinaki D, Bowles KM, Katzenellenbogen E, Knirel YA, Whitfield C, Nishimura T, Matsumoto N, Yamamoto K, Iwakura Y, Saijo S, Kawasaki N.
  • Journal Title: J Biol Chem. Volume: 291 Pages: 17629-17638
  • DOI: 10.1074/jbc.m116.741256
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The innate immune receptor Dectin-2 mediates the phagocytosis of cancer cells by Kupffer cells for the suppression of liver metastasis. (2016)
  • Author(s): Kimura Y., Inoue A.., Hangai S.., Saijo S., Negishi H., Nishio J., Yamasaki S., Iwakura Y., Yanai H., Taniguchi T.
  • Journal Title: Proceedings of National Academy of Sciences of the United States of America Volume: 113 Pages: 14097-14102
  • DOI: 10.1073/pnas.1617903113
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Roles of Dectin-1 and Dectin-2 in anti-fungal immunity (2017)
  • Author(s): Shinobu Saijo
  • Organizer: International Cytokine and Interferon Society
  • Int'l Joint Research / Invited
• [Presentation] 真菌によるIL－17産生機構 (2016)
  • Author(s): 西城 忍
  • Organizer: AMED-CREST若手研究会
  • Place of Presentation: 大阪アカデミア （大阪府大阪市）
  • Year and Date: 2016-06-15
  • Invited
• [Presentation] 真菌の細胞壁構成成分とC型レクチン受容体による認識 (2016)
  • Author(s): 西城 忍
  • Organizer: 第８１回日本インターフェロン・サイトカイン学会
  • Place of Presentation: 長崎大学良順会館 （長崎県長崎市）
  • Year and Date: 2016-05-13
  • Invited
• [Remarks] 千葉大学真菌医学研究センター 感染免疫分野・サイトカインプロジェクト
  • URL: http://cytokine.pf.chiba-u.jp/
• [Remarks] 千葉大学真菌医学研究センター感染免疫分野
  • URL: http://cytokine.pf.chiba-u.jp/