Exosomal miRNA as a marker of brown adipose tissue in humans
| Project/Area Number |
16K15485
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAITO MASAYUKI 北海道大学, 獣医学研究科, 名誉教授 (80036441)
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| Co-Investigator(Kenkyū-buntansha) |
松下 真美 天使大学, 看護栄養学部, 助教 (60517316)
岡松 優子 北海道大学, 獣医学研究院, 講師 (90527178)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 褐色脂肪組織 / 血中マーカー / miRNA / エクソソーム / FDG-PET/CT / miRNA-92a / 肥満 / エネルギー代謝 / 脂肪組織 |
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| Outline of Final Research Achievements |
Brown adipose tissue (BAT) is a site specific for non-shivering thermogenesis during cold exposure and food intake, contributing to the autonomic regulation of expenditure and body fat content. To develop a simple and noninvasive method of assessing BAT in humans, serum exosomal miRNA was analyzed with special reference to the BAT activity assessed by the standard FDG-PET/CT method, which has serious limitation s including the enormous cost and radiation exposure. An extensive array and PCR analyses of serum sample obtained from 40 healthy volunteer subjects revealed that the concentration of 11 miRNA species, particularly that of miRNA122-5p, was significantly lower in subjects with higher BAT activities. These results suggest that serum miRNA122-5p may be a quantitative index of BAT activity in humans.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Brown adipose tissue is involved in diet-induced thermogenesis and whole-body fat utilization in healthy humans2016
Author(s)
Hibi H, Oishi S, Matsushita M, Yoneshiro T, Yamaguchi T, Usui C, Yasunaga K, Katsuragi Y, Kubota K, Tanaka S, Saito M
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Journal Title
Int J Obes
Volume: 40
Issue: 11
Pages: 1655-1661
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Diurnal variation of postprandial thermogenesis and substrate oxidation in humans: Involvement of brown adipose tissue2017
Author(s)
Mami Matsushita, Masanobu Hibi, Sachiko Oishi, Takeshi Yoneshiro, Tohru Yamaguchi, Chiyoko Usui, Koichi Yasunaga, Yoshihisa Katsuragi, Kazuo Kubota, Shigeho Tanaka, Masayuki Saito
Organizer
RACMEM 2017
Related Report
Int'l Joint Research
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[Presentation] Brown fat in human obesity2016
Author(s)
Masayuki Saito
Organizer
International Congress on Obesity
Place of Presentation
Vancouver Convention Centre, Vancouver, Canada
Year and Date
2016-05-01
Related Report
Int'l Joint Research / Invited
