| Project/Area Number |
16K15492
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SAWATUBASHI Shun 徳島大学, 藤井節郎記念医科学センター, 講師 (70535103)
TAKEMOTO Tatsuya 徳島大学, 藤井節郎記念医科学センター, 助教 (30443899)
YAMAMOTO Yasuhiko 金沢大学, 大学院医歯薬保健学総合研究科, 教授 (20313637)
TAKAGI Kiyoshi 東北大学, 大学院医学系研究科, 助教 (80595562)
Sato Horoshi 東北大学, 大学院薬学研究科, 教授 (60215829)
Yokoyama Atsushi 東北大学, 大学院医学系研究科, 助教 (20572332)
ITO Ryo 東北大学, 大学院医学系研究科, 助教 (80733815)
DOI Takayuki 東北大学, 大学院薬学研究科, 教授 (90212076)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | CRISPR/Cas9システム / ChREBP / 糖尿病性腎症 / ハイスループットスクリーニング / 化合物ライブラリー / 糖質応答転写因子 / 創薬 / スクリーニング |
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| Outline of Final Research Achievements |
1) Generation of ChREBP KO mice. We have succeeded in generating ChREBP KO mice (C57BL/6J) using CRISPR/Cas9 system. 2) Generation of high throughput screening (HTS) system using MES13 ChoRE-Luc cells. We have succeeded in generating HTS system using the cell line by obtaining Z score above 0.5. 3) HTS of Tohoku University chemical library: We obtained 13 hit chemicals among 5,861 compounds. Among them, we selected 1 compound (X) for the further investigation. 4) Administration of chemical compounds to Ins-iNOS-transgenic mice: We are now administrating the compound X to the mice.
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