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SIngle-cell transcriptional networks during B cell differentiation

Research Project

Project/Area Number 16K15506
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Hematology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Ikawa Tomokatsu  国立研究開発法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (60450392)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsB細胞分化 / 1細胞発現解析 / 運命決定 / 転写因子 / 造血幹細胞 / B細胞 / 分化 / 1細胞
Outline of Final Research Achievements

B cells are generated from hematopoietic stem cells (HSCs) through a successive series of lineage restriction processes. Although many transcription factors were shown to control the B cell fate determination, the exact mechanisms remain largely unknown. We have recently established an ideal system that can be used to examine gene regulatory networks during lymphoid lineage commitment from HSCs. In this study, we focused on the gene expression profiles at the single cell level during B cell differentiation using this system. The single cell RNA-seq analysis using the in vitro culture system demonstrated the step-wise activation of B-lineage associated genes, whereas the genes involved in the multipotency were gradually repressed. The gene expression patterns obtained by the culture system totally fit with the data of the single progenitors isolated from mouse bone marrow. These results will provide a blueprint for studying the normal and neoplastic development of B cells.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (20 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 11 results,  Invited: 6 results)

  • [Journal Article] Kelch-like protein 14 promotes B-1a but suppresses B-1b cell development.2018

    • Author(s)
      1.Li S, Liu J, Min Q, Ikawa T, Yasuda S, Yang Y, Wang YQ, Tsubata T, Zhao Y, Wang JY.
    • Journal Title

      Int Immunol

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Three-step transcriptional priming that drives the commitment of multipotent progenitors toward B cells.2018

    • Author(s)
      2.Miyai T, Takano J, Endo TA, Kawakami E, Agata Y, Motomura Y, Kubo M, Kashima Y, Suzuki Y, Kawamoto H, Ikawa T.
    • Journal Title

      Genes Dev

      Volume: 32 Pages: 112-126

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] CAGE profiles of human and mouse samples.2017

    • Author(s)
      3.Noguchi S, Arakawa T,…, Ikawa T (75 out of 178),…, Kawaji H*, Carninci P, Forrest ARR, Hayashizaki Y.
    • Journal Title

      Sci Data. 4: 170112

      Volume: 4 Pages: 170112-170112

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Conversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program2016

    • Author(s)
      Ikawa T, Masuda K, Endo TA, Endo M, Isono K, Koseki Y, Nakagawa R, Kometani K, Takano J, Agata Y, Katsura Y, Kurosaki T, Vidal M, Koseki H, Kawamoto H
    • Journal Title

      Genes & Development

      Volume: 30 Pages: 2475-2485

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Transient Tcf3 gene repression by TALE-tanscription factor targeting2016

    • Author(s)
      Masuda J, Kawamoto H, Strober W, Takayama-E, Mizutani A, Murakami H, Ikawa T, Kitani A, Maeno N, Shigehiro T, Satoh A, Seno A, Arun V, Kasai T, Fuss IJ, Katsura Y, Seno M
    • Journal Title

      Appl Biochem Biotechnol

      Volume: 180 Pages: 1559-1573

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Immunosystem development2017

    • Author(s)
      Ikawa T
    • Organizer
      日本免疫学会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Three-step transcriptional priming that drives multipotent progenitors toward B cells.2017

    • Author(s)
      Ikawa T, Miyai T, Takano J
    • Organizer
      日本免疫学会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Epigenetic maintenance of T cell identity by Polycomb-mediated suppression of Pax5.2017

    • Author(s)
      Ikawa T
    • Organizer
      BMB2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] “リンパ球分化とエピジェネティクス.” 大阪大学 微研・部員会共催セミナー2017

    • Author(s)
      Ikawa T
    • Organizer
      大阪大学
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] A road map that guides the development of B cells.2017

    • Author(s)
      Ikawa T, Miyai T and Takano J.
    • Organizer
      The 3rd Tsinghua-RIKEN Joint Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Transcriptional networks during B lymphocyte commitment.2017

    • Author(s)
      Ikawa T, Miyai T and Takano J
    • Organizer
      FASEB meeting
    • Related Report
      2017 Annual Research Report
  • [Presentation] A challenge as a young chief investigator in IMS2017

    • Author(s)
      Ikawa T
    • Organizer
      RIKEN Sakura Symposium 2017.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of gene regulatory networks during hematopoietic stem cell differentiation by the single-cell analysis2017

    • Author(s)
      Ikawa T
    • Organizer
      RIKEN Single Cell Workshop 2017.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A critical role of non-canonical PRC1 for specification to lymphoid lineage2017

    • Author(s)
      Tomokatsu Ikawa
    • Organizer
      KTCC 2017
    • Place of Presentation
      京都大学(京都府・京都市)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 異性型ポリコーム複合体による造血幹細胞分化制御2016

    • Author(s)
      伊川 友活
    • Organizer
      さきがけ終了領域報告会
    • Place of Presentation
      九州大学(福岡県・福岡市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Dynamic transcriptional cascades and epigenetic regulation that orchestrate B cell fate determination2016

    • Author(s)
      Junichiro Takano, Tomohiro Miyai, Tomokatsu Ikawa
    • Organizer
      日本免疫学会総会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Dynamic transcriptional cascades and epigenetic regulation that orchestrate B cell fate determination2016

    • Author(s)
      Junichoro Takano, Tomohiro Miyai, Haruhiko Koseki, Tomokatsu Ikawa
    • Organizer
      日本血液学会学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Transcriptional networks that establish B cell identity2016

    • Author(s)
      Tomokatsu Ikawa
    • Organizer
      International Congress of Immunology 2016
    • Place of Presentation
      Melbourne (Australia)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 異性型Polycomb repressive complexは造血幹細胞からリンパ球系列への運命決定に重要である2016

    • Author(s)
      高野 淳一郎、古関 明彦、伊川 友活
    • Organizer
      第26回KTCC
    • Place of Presentation
      延暦寺会館(京都府・京都市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Transcriptional reglatory networks that regulate B cell fate determination2016

    • Author(s)
      Tomokatsu Ikawa
    • Organizer
      CSHL meeting
    • Place of Presentation
      Cold Spring Harbor (USA)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-03-29  

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