Elucidation of oxygen-sensing molecular mechanisms on fetal pulmonary artery and ductus arteriosus

• Project/Area Number: 16K15537
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Jikei University School of Medicine
• Principal Investigator: AKAIKE Toru 東京慈恵会医科大学, 医学部, 講師 (20647101)
• Co-Investigator(Kenkyū-buntansha): 南沢 享 東京慈恵会医科大学, 医学部, 教授 (40257332)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 肺動脈 / 動脈管 / シグナル伝達 / 酸素感知機構

Outline of Final Research Achievements

In this study, we determined what factors dominantly expressed by an increase of oxygen concentration expressed in fetal rat pulmonary arterial and ductus arteriosus smooth muscle cells. Fetal rat pulmonary arterial and ductus arteriosus smooth muscle cells incubated under hypoxia for 48hours, and then incubated under normoxia for 24hours. The total RNA was extracted from these cells and was exhaustively gene-analyzed. DNA microarray revealed that transmembrane protein 104 and olfactory receptor 1262 were up-regulated by an increase of oxygen concentration in fetal rat pulmonary arterial and ductus arteriosus smooth muscle cells. And ankyrin repeat domain 37 was down-regulated by an increase of oxygen concentration in fetal rat pulmonary arterial and ductus arteriosus smooth muscle cells. We will continue to investigate the molecular mechanism of these gene factors.

Research Products

• [Journal Article] A Sarcoplasmic Reticulum Localized Protein Phosphatase Regulates Phospholamban Phosphorylation and?Promotes Ischemia Reperfusion Injury?in?the Heart (2017)
  • Author(s): Akaike Toru、Du Na、Lu Gang、Minamisawa Susumu、Wang Yibin、Ruan Hongmei
  • Journal Title: J Am Coll Cardiol Basic Trans Science Volume: 2 Issue: 2 Pages: 160-180
  • DOI: 10.1016/j.jacbts.2016.12.002
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tissue thrombin is associated with the pathogenesis of dilated cardiomyopathy. (2016)
  • Author(s): Ito K, Hongo K, Date T, Ikegami M, Hano H, Owada M, Morimoto S, Kashiwagi Y, Katoh D, Yoshino T, Yoshii A, Kimura H, Nagoshi T, Kajimura I, Kusakari Y, Akaike T, Minamisawa S, Ogawa K, Minai K, Ogawa T, Kawai M, Yajima J, Matsuo S, Yamane T, Taniguchi I, Morimoto S, Yoshimura M.
  • Journal Title: Int J Cardiol. Volume: 228 Pages: 821-827
  • DOI: 10.1016/j.ijcard.2016.11.176
  • Peer Reviewed / Open Access
• [Journal Article] Impairment of excitation-contraction coupling in right ventricular hypertrophied muscle with fibrosis induced by pulmonary artery banding. (2016)
  • Author(s): Kusakari Y, Urashima T, Shimura D, Amemiya E, Miyasaka G, Yokota S, Fujimoto Y, Akaike T, Inoue T, Minamisawa S．
  • Journal Title: PLoS One. Volume: 12 Issue: 1 Pages: e0169564-e0169564
  • DOI: 10.1371/journal.pone.0169564
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 鳥類の動脈管閉鎖におけるインドメタシンの作用. (2018)
  • Author(s): 赤池 徹, 南沢 享.
  • Organizer: 第95回日本生理学会大会
• [Presentation] ゲンタマイシンによる新生仔ラット動脈管閉鎖への影響. (2017)
  • Author(s): 岸渕安也名, 赤池 徹, 南沢 享.
  • Organizer: 第94回日本生理学会大会
• [Presentation] Inhibition of Cyclooxygenase Contracts Chicken Ductus Arteriosus. (2017)
  • Author(s): Akaike T, Minamisawa S.
  • Organizer: The 8th TAKAO International Symposium on Molecular Mechanism of Cardiopulmonary Disease
  • Int'l Joint Research
• [Presentation] Prostaglandin E2 receptor EP4 inhibition constricts the rat ductus arteriosus. (2017)
  • Author(s): Sakuma T, Akaike T, Minamisawa S.
  • Organizer: The 8th TAKAO International Symposium on Molecular Mechanism of Cardiopulmonary Disease
  • Int'l Joint Research