Development of heart failure treatment by new device using extracellular matrix modulating cardiac stem cell niche

• Project/Area Number: 16K15631
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Osaka University
• Principal Investigator: Sawa Yoshiki 大阪大学, 医学系研究科, 教授 (00243220)
• Co-Investigator(Kenkyū-buntansha): 増田 茂夫 大阪大学, 医学系研究科, 特任准教授(常勤) (10396749) ; 齋藤 充弘 大阪大学, 医学系研究科, 特任准教授(常勤) (20448038) ; 寒川 延子 大阪大学, 医学(系)研究科(研究院), その他 (30432579) ; 福嶌 五月 国立研究開発法人国立循環器病研究センター, 病院, 医長 (80596867) ; 秦 広樹 大阪大学, 医学系研究科, 講師 (80638198)
• Research Collaborator: SEKIGUCHI Kiyotoshi
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 幹細胞ニッチ / 細胞外マトリックス / 組織修復 / 細胞外基質 / 心不全

Outline of Final Research Achievements

Mesenchymal stem cells (MSCs) showed strong adhesive and proliferation activity on LM511. To examine the effects of LM511 in vivo, we transplanted LM511 in rat acute myocardial infarction model. Four weeks later, the number of MSCs or that of endothelial cells were significantly higher in the LM511-transplanted group than in the control group. The number of them increased further over four to eight weeks. In addition, transplantation of LM511 increased the expression of angiogenic cytokines. Moreover transplantation of LM511 was induced suppression of fibrosis and improvement of cardiac function. Our data indicate that transplantation of the LM511 may contribute to localization of MSCs to the implantation site and to modulating stem cell activities, leading to angiogenesis and improvement of the cardiac function in the rat acute myocardial infarction heart. Supplementation of components of the cardiac stem cell niche may be a promising new approach in the treatment of heart disease.

Research Products

• [Presentation] 急性心筋梗塞に対するラミニン511含有アテロコラーゲンシート移植の有用性の検討 (2018)
  • Author(s): 寒川延子、宮川繁、福嶌五月、齋藤充弘、横山淳也、原田明希摩、小田ー望月紀子、佐藤（西内）涼子、関口清俊、澤芳樹
  • Organizer: 第17回 日本再生医療学会総会
• [Presentation] Novel stem cell niche Laminin-511 promotes functional angiogenesis through enhanced stem cell homing by modulating Stem cell "niche" in the failed heart (2017)
  • Author(s): Sougawa N, Miyagawa S, Fukushima S, Saito A, Yokoyama J, Kitahara M, Harada A, Sato-Nishiuchi R, Sekiguchi K, Sawa Y
  • Organizer: American Heart Association Scientific Sessions 2017
  • Int'l Joint Research