Elucidation for Histone induced cell death in sepsis
| Project/Area Number |
16K15681
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山崎 創 東邦大学, 医学部, 准教授 (70315084)
中野 裕康 東邦大学, 医学部, 教授 (70276476)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANO Hiroyasu 東邦大学, 医学部, 教授 (70276476)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Histone / sepsis / cell death / gene trap / ヒストン / 細胞死 / 敗血症 / Gene-trap / CRISPR/Cas9 / CRISPR-Cas9 |
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| Outline of Final Research Achievements |
Circulating histones are major mediators of sepsis and cytotoxic to vascular endothelium. For analyzing cytotoxic mechanism of histones, we constructed a haploid genetic screen system using HAP1 cells. Infection efficiency of gene-trap vector to HAP1 cells was 32%, it revealed that high frequency integration was occurred in gene structures. We treated gene-trapped HAP1 cells with histones, surviving cells were treated with histones again.
We could not find a mediator gene of histone toxicity, but histones have possibilities medical targets for sepsis.
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] Depletion of myeloid cells exacerbates hepatitis and induces an aberrant increase in histone H3 in mouse serum2017
Author(s)
Piao X, Yamazaki S, Komazawa-Sakon S, Miyake S, Nakabayashi O, Kurosawa T, Mikami T, Tanaka M, Van Rooijen N, Ohmuraya M, Oikawa A, Kojima Y, Kakuta S, Uchiyama Y, Tanaka M, Nakano H.
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Journal Title
Hepatology
Volume: 65
Pages: 237-252
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains.2017
Author(s)
Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, Tanaka K, Takahashi H, Sawasaki T, Inoue S, Oshiumi H, Seya T, Nakano H, Tanaka Y, Iwai K, Inoue J.
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Journal Title
PLoS Pathog
Volume: 13:e1006162
Pages: 6162-6163
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Novel method to rescue a lethal phenotype through integration of target gene onto the X-chromosome2016
Author(s)
Sakata K, Araki K, Nakano H, Nishina T, Komazawa-Sakon S, Murai S, Lee GE, Hashimoto D, Suzuki C, Uchiyama Y, Notohara K, Gukovskaya AS, Gukovsky I, Yamamura KI, Baba H, Ohmuraya M.
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Journal Title
Sci Rep
Volume: 6
Pages: 37200-37200
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] 細胞死制御因子cFLIPの肝細胞特異的欠損マウスを用いた肝障害モデルにおけるクッパー細胞および骨髄由来細胞の役割2016
Author(s)
朴 雪花, ◎山﨑 創, 駒澤-左近 幸子, 三宅 早苗, 中林 修, 田中 稔, 大村谷 昌樹, 及川 彰, 角田 宗一郎, 内山 安男, 田中 正人, 中野 裕康
Organizer
第89回日本生化学会大会
Place of Presentation
東北大学川内北キャンパス(宮城県・仙台市)
Year and Date
2016-09-26
Related Report
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