Understanding of dental plaque microbiota in the early stage

• Project/Area Number: 16K15856
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: Kyushu University
• Principal Investigator: Takeshita Toru 九州大学, 歯学研究院, 准教授 (50546471)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 初期付着細菌 / デンタルプラーク / マイクロバイオーム / 16S rRNA / 口腔 / う蝕 / 初期プラーク / 口腔マイクロバイオーム / 唾液 / 舌苔 / 16S rRNA遺伝子 / メタゲノム

Outline of Final Research Achievements

Dental plaque is a dynamic microbial biofilm of which the develpment begins with adherence of selected bacteria. This study aimed to understand dental plaque in the early stage. Plaque samples that accumulated on a hydroxyapatite disk for 6 hours were collected from the subjects using a hydroxyapatite disk and analyzed using an open-ended approach using bacterial 16S rRNA gene. This study revealed the detailed bacterial composition of dental plaque in an early stage and identified the predominant bacterial taxa with strong adhesion ability on tooth surfaces. The initial dental plaque assoicated with dental caries was also characterized. Our results would be an important basis for a development of an effective approach to promote oral health.

Research Products

• [Journal Article] Relationships of variations in the tongue microbiota and pneumonia mortality in nursing home residents (2018)
  • Author(s): Kageyama S, Takeshita T, Furuta M, Tomioka M, Asakawa M, Suma S, Takeuchi K, Shibata Y, Iwasa Y, Yamashita Y
  • Journal Title: J Gerontol A Biol Sci Med Sci Volume: - Issue: 8 Pages: 1097-1102
  • DOI: 10.1093/gerona/glx205
  • Peer Reviewed
• [Journal Article] The oral microbiome and human health (2017)
  • Author(s): Yamashita Y, Takeshita T
  • Journal Title: Journal of Oral Science Volume: 59 Issue: 2 Pages: 201-206
  • DOI: 10.2334/josnusd.16-0856
  • NAID: 130006882396
  • ISSN: 1343-4934, 1880-4926
  • Peer Reviewed
• [Journal Article] Relative abundance of total subgingival plaque-specific bacteria in salivary microbiota reflects the overall periodontal condition in patients with periodontitis (2017)
  • Author(s): Kageyama S, Takeshita T, Asakawa M, Shibata Y, Takeuchi K, Yamanaka W, Yamashita Y
  • Journal Title: PLoS One Volume: 12 Issue: 4 Pages: e0174782-e0174782
  • DOI: 10.1371/journal.pone.0174782
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] デンタルプラーク形成に関わる初期付着細菌群の特定 (2017)
  • Author(s): 竹下徹、井原由佳理、影山伸哉、松見理恵、山下喜久
  • Organizer: 第91回日本細菌学会総会
• [Presentation] Dental plaque development on a hydroxyapatite disk in young adults observed by using a barcoded pyrosequencing (2016)
  • Author(s): 竹下 徹
  • Organizer: 第65回日本口腔衛生学会総会
  • Place of Presentation: 東京
  • Invited