Suppression of arthritis by photodynamic therapy in rheumatoid arthritis model mice.
| Project/Area Number |
16K16405
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical systems
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Onishi Shinzo 筑波大学, 附属病院, 病院講師 (00633190)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | photodynamic therapy / ICG Lactosome / ICG / arthritis / nano particle / 光線力学療法 / ICGラクトソーム / 蛍光イメージング / ナノ粒子 / 関節炎 / 関節リウマチ / 血管透過性亢進 / 関節リウマチモデルマウス / 内科 / ナノ材料 |
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| Outline of Final Research Achievements |
1. Photodynamic therapy using ICG lactosomes (ICGL) was performed toward ankle joint arthritis in RA mice.
ICGL contained ICG 30 nmol / mg in 2 mg. The laser irradiation was 125 mW / cm2, and the total irradiation energy density was 75.2 J / cm2. TUNEL staining was used for necrotic cell evaluation. There was a significant difference in the number of positive cells in the photodynamic therapy group compared with the control group. 2. In ICGL dosage, we compared with 5 mg, 10 mg, 20 mg. In 5 mg decreased fluorescence luminance was seen after irradiation compared with 10mg and 20mg. But in 20mg, Skin erosion appeared. So 10 mg was suit for multiple irradiation. 3. In multiple irradiation, ICGL amount in the body was greatly decreased by two irradiations, and almost disappeared by three irradiations.
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Report
(3 results)
Research Products
(1 results)
