Development of new method for screening anticancer drugs that target topoisomerases by using DNA origami

• Project/Area Number: 16K17934
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Kyoto University
• Principal Investigator: Arivazhagan Rajendran 京都大学, エネルギー理工学研究所, 講師 (90723122)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: DNA origami / Inhibitors / Topoisomerase / HS-AFM / Drug screening / Single-molecule analysis / Rotaxane / Catenane / DNA rotaxanes / DNA catenane / Topoisomerase inhibitors / high speed AFM / ナノバイオ / High-Speed AFM

Outline of Final Research Achievements

The supramolecular assemblies of the topologically interlocked components inside a DNA origami were constructed. Such assemblies of the functional structures are promising in the fields of molecular switches, motors, sensors, and logic devices.

Research Products

• [Int'l Joint Research] Eiwa Womens University(韓国)
• [Journal Article] One-pot Isolation of Desired Human Genome Fragment by Using Biotinylated pcPNA/S1 Nuclease Combination (2018)
  • Author(s): Arivazhagan Rajendran, Narumi Shigi, Jun Sumaoka, and Makoto Komiyama
  • Journal Title: Biochemistry Volume: 57 Issue: 20 Pages: 2908-2912
  • DOI: 10.1021/acs.biochem.8b00202
  • Peer Reviewed
• [Journal Article] Nucleic acids-templated enzyme cascades (2017)
  • Author(s): A. Rajendran, E. Nakata, S. Nakano, T. Morii
  • Journal Title: ChemBioChem Volume: 18 Issue: 8 Pages: 696-716
  • DOI: 10.1002/cbic.201600703
  • Peer Reviewed
• [Presentation] Supramolecular assemblies of mechanically interlocked components inside a DNA origami frame (2018)
  • Author(s): A. Rajendran, S. Park, E. Nakata, Y. Kwon, T. Morii
  • Organizer: The 98th Annual meeting of The chemical society of Japan
• [Presentation] Topologically interlocked DNA nanostructures inside a DNA origami frame (2017)
  • Author(s): A. Rajendran, S. Park, E. Nakata, Y. Kwon, Takashi Morii
  • Organizer: The 97th Annual meeting of The chemical society of Japan
  • Place of Presentation: Keio University, Yokohama
  • Year and Date: 2017-03-16
• [Presentation] DNA rotaxane and catenane inside a DNA nanostructure (2017)
  • Author(s): A. Rajendran, S. Park, E. Nakata, Y. Kwon, T. Morii
  • Organizer: The 2nd International Symposium on Biofunctional Chemistry
  • Int'l Joint Research
• [Presentation] Construction of topologically interlocked DNAs inside a DNA scaffold (2017)
  • Author(s): A. Rajendran, S. Park, E. Nakata, Y. Kwon, T. Morii
  • Organizer: The 44th International symposium on nucleic acids chemistry and 1st Annual meeting of Japan society of nucleic acids chemistry
  • Int'l Joint Research
• [Presentation] DNA nanotechnology and it’s medicinal applications (2017)
  • Author(s): A. Rajendran
  • Organizer: 2017 International Conference on Environmental Medicine and the 14th Symposium of the Frontiers of Biomedical Sciences
  • Int'l Joint Research / Invited
• [Presentation] Assembly of DNA rotaxane and catenane inside a DNA origami frame (2017)
  • Author(s): A. Rajendran, S. Park, E. Nakata, Y. Kwon, T. Morii
  • Organizer: The 11th Symposium on bio-relevant chemistry
• [Presentation] Topologically interlocked DNA nanostructures inside a DNA origami frame (2017)
  • Author(s): A. Rajendran, S. Park, E. Nakata, Y. Kwon, T. Morii
  • Organizer: The 97st Annual meeting of The chemical society of Japan
• [Remarks] Research web page
  • URL: https://drraj.webnode.com/