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Deciphering a hypothalamo-hippocampal circuit in goal-directed spatial navigation

Research Project

Project/Area Number 16K18373
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurophysiology / General neuroscience
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

陳 碩  国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (30772694)

Project Period (FY) 2016-04-01 – 2017-03-31
Project Status Discontinued (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsGoal-directed navigation / Decision-making / Optogenetics / Neural circuits / Hippocampus / Hypothalamus / hippocampus / spatial navigation / optogenetics / electrophysiology
Outline of Annual Research Achievements

Decision-making during goal-directed navigation is a highly evolved behavior requiring a coordination of perception, learning and memory, and planning. In the past five months, by using an array of circuit interrogation techniques including viral tracing and optogenetics, we have successfully identified a crucial role of the neural circuit from hypothalamic supramammillary nucleus (SuM) to hippocampal dentate gyrus (DG) in mice performing a T-maze delayed non-match to sample task. This significant progress has filled the gap on a wide circuit map that bridge to the hippocampus for making decisions during spatial navigation.
At first, we performed brain-wide 3D mapping of SuM efferents by taking advantage of a SuM-Cre transgenic mouse line that allows specific expression of fluorescent proteins at SuM. After brain tissue clearing, high-resolution reconstruction of an atlas of SuM efferents was achieved, indicating strong projections from SuM to DG.
Next, we employed targeted optogenetics to test the function of SuM-DG circuit in goal-directed navigation. Cre-dependent channelrhodopsin-2 (ChR2) or archaerhodopsin (ArchT)-expressing adeno-associated virus (AAV) was injected to SuM. For the ChR2-transfected mice, application of 488 nm laser at DG terminals showed no effect on the task performance. However, when 532 nm laser was applied to the ArchT-transfected mice, we found a dramatic decline of the T-maze performance. This result clearly indicated that inhibition of the SuM-DG circuit impaired the animal’s spatial working memory during goal-directed navigation.

Report

(1 results)
  • 2016 Annual Research Report
  • Research Products

    (5 results)

All 2016

All Presentation (5 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Presentation] Go Social or Spatial: Divergent Information Routing by a Hypothalamo-Hippocampal Circuit in Working Memory2016

    • Author(s)
      Shuo Chen
    • Organizer
      Gordon Research Seminar Neurobiology of Cognition
    • Place of Presentation
      Newry, Maine, USA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Go Social or Spatial: Divergent Information Routing by a Hypothalamo-Hippocampal Circuit in Working Memory2016

    • Author(s)
      Shuo Chen, Yanqiu Tao, Marie Wintzer, Denis Polygalov, Arthur Huang, Roman Boehringer, Jingyi Chen, Thomas McHugh
    • Organizer
      Gordon Research Conference Neurobiology of Cognition
    • Place of Presentation
      Newry, Maine, USA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Go Social or Spatial: Divergent Information Routing by a Hypothalamo-Hippocampal Circuit in Working Memory2016

    • Author(s)
      Shuo Chen, Yanqiu Tao, Marie Wintzer, Denis Polygalov, Arthur Huang, Roman Boehringer, Jingyi Chen, Thomas McHugh
    • Organizer
      Gordon Research Conference Optogenetic Approaches to Understanding Neural Circuits & Behavior
    • Place of Presentation
      Newry, Maine, USA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optogenetic Dissection of Selective Information Routing by a Hypothalamo-Hippocampal Circuit2016

    • Author(s)
      Yanqiu Tao, Shuo Chen, Denis Polygalov, Arthur Huang, Roman Boehringer, Jingyi Chen, Thomas McHugh
    • Organizer
      The 39th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Pacifico Yokohama, Yokohama, Japan
    • Related Report
      2016 Annual Research Report
  • [Presentation] Optogenetic Dissection of Selective Information Routing by a Hypothalamo-Hippocampal Circuit2016

    • Author(s)
      Yanqiu Tao, Shuo Chen, Arthur Huang, Marie Wintzer, Denis Polygalov, Roman Boehringer, Jingyi Chen, Thomas McHugh
    • Organizer
      Neuroscience 2016, SfN's 46th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2018-01-16  

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