Development of disease-modeling technology using hierarchical genome/epigenome editing

• Project/Area Number: 16K18478
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: Hiroshima University
• Principal Investigator: SAKUMA TETSUSHI 広島大学, 理学研究科, 特任講師 (90711143)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: がん / ゲノム編集 / エピゲノム編集 / マイクロホモロジー媒介性末端結合 / MMEJ / ゲノム生物工学 / CRISPR-Cas9 / TALEN

Outline of Final Research Achievements

In this research, we developed the genome/epigenome editing technologies enabling disease modeling caused by genomic and/or epigenomic abnormality that multiplexly occurs in a single cell. Regarding genome editing, we achieved the improvement of MMEJ-dependent gene knock-in (PITCh) and the development of automated design tool of PITCh knock-in. In addition, we realized simultaneous gene knock-in into multiple gene loci using the improved PITCh system. Regarding epigenome editing, we constructed the TREE system for hyper accumulation of effector molecules, showing superior editing effect compared to the conventional systems. Collectively, the technology capable of modeling complex multifactorial pathogenesis was established in terms of both genome and epigenome editing.

Research Products

• [Journal Article] PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetero-oligomerization through interaction with Bid in human colon cancer (2018)
  • Author(s): Kuriyama S, Tsuji T, Sakuma T, Yamamoto T, Tanaka M
  • Journal Title: Cell Death Discovery Volume: 4 Issue: 1 Pages: 11-11
  • DOI: 10.1038/s41420-017-0006-5
  • Peer Reviewed / Open Access
• [Journal Article] Microhomology-assisted scarless genome editing in human iPSCs (2018)
  • Author(s): Kim Shin-Il、Matsumoto Tomoko、Kagawa Harunobu、Nakamura Michiko、Hirohata Ryoko、Ueno Ayano、Ohishi Maki、Sakuma Tetsushi、Soga Tomoyoshi、Yamamoto Takashi、Woltjen Knut
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 939-939
  • DOI: 10.1038/s41467-018-03044-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes. (2018)
  • Author(s): Shukunami C, Takimoto A, Nishizaki Y, Yoshimoto Y, Tanaka S, Miura S, Watanabe H, Sakuma T, Yamamoto T, Kondoh G, Hiraki Y.
  • Journal Title: Scientific reports Volume: 8 Issue: 1 Pages: 1-12
  • DOI: 10.1038/s41598-018-21194-3
  • NAID: 120006522882
  • Peer Reviewed / Open Access
• [Journal Article] Generation of D1-1 TALEN isogenic control cell line from Dravet syndrome patient iPSCs using TALEN-mediated editing of the SCN1A gene (2018)
  • Author(s): Tanaka Yasuyoshi、Sone Takefumi、Higurashi Norimichi、Sakuma Tetsushi、Suzuki Sadafumi、Ishikawa Mitsuru、Yamamoto Takashi、Mitsui Jun、Tsuji Hitomi、Okano Hideyuki、Hirose Shinichi
  • Journal Title: Stem Cell Research Volume: 28 Pages: 100-104
  • DOI: 10.1016/j.scr.2018.01.036
  • Peer Reviewed / Open Access
• [Journal Article] Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus (2018)
  • Author(s): Sakuma T, Mochida K, Nakade S, Ezure T, Minagawa S, Yamamoto T.
  • Journal Title: Genes Cells Volume: 23 Issue: 4 Pages: 255-263
  • DOI: 10.1111/gtc.12569
  • Peer Reviewed
• [Journal Article] Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference (2018)
  • Author(s): Sato'o Y, Hisatsune J, Yu L, Sakuma T, Yamamoto T, Sugai M.
  • Journal Title: PLoS One Volume: 13 Issue: 1 Pages: e0185987-e0185987
  • DOI: 10.1371/journal.pone.0185987
  • Peer Reviewed / Open Access
• [Journal Article] Genome editing for dissecting and curing human genetic diseases (2018)
  • Author(s): Sakuma T, Yamamoto T.
  • Journal Title: J Hum Genet Volume: 63 Issue: 2 Pages: 105-105
  • DOI: 10.1038/s10038-017-0380-0
  • Peer Reviewed
• [Journal Article] Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems (2018)
  • Author(s): Kawabe Y, Komatsu S, Komatsu S, Murakami M, Ito A, Sakuma T, Nakamura T, Yamamoto T, Kamihira M.
  • Journal Title: J Biosci Bioeng Volume: 125 Issue: 5 Pages: 599-605
  • DOI: 10.1016/j.jbiosc.2017.12.003
  • NAID: 40021552678
  • Peer Reviewed
• [Journal Article] Cancer induction and suppression with transcriptional control and epigenome editing technologies. (2017)
  • Author(s): Nakade S, Yamamoto T, Sakuma T.
  • Journal Title: Journal of human genetics Volume: 1 Issue: 2 Pages: 187-194
  • DOI: 10.1038/s10038-017-0377-8
  • Peer Reviewed
• [Journal Article] ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair. (2017)
  • Author(s): Tsuda M, Cho K, Ooka M, Shimizu N, Watanabe R, Yasui A, Nakazawa Y, Ogi T, Harada H, Agama K, Nakamura J, Asada R, Fujiike H, Sakuma T, Yamamoto T, Murai J, Hiraoka M, Koike K, Pommier Y, Takeda S, Hirota K.
  • Journal Title: PLos One Volume: 12 Issue: 11 Pages: e0188320-e0188320
  • DOI: 10.1371/journal.pone.0188320
  • NAID: 120006800808
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology (2017)
  • Author(s): Abe S, Kobayashi K, Oji A, Sakuma T, Kazuki K, Takehara S, Nakamura K, Okada A, Tsukazaki Y, Senda N, Honma K, Yamamoto T, Ikawa M, Chiba K, Oshimura M, Kazuki Y.
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 15189-15189
  • DOI: 10.1038/s41598-017-15033-0
  • NAID: 120006957433
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of pten knockout medaka with transcription activator-like effector nucleases (TALENs) as a model of PTEN deficiency disease (2017)
  • Author(s): Matsuzaki Y, Sakuma T, Yamamoto T, Saya H.
  • Journal Title: PLoS One Volume: 12 Issue: 10 Pages: e0186878-e0186878
  • DOI: 10.1371/journal.pone.0186878
  • Peer Reviewed / Open Access
• [Journal Article] TALEN-mediated targeted editing of the GDE5 gene suppresses fibroblastic cell proliferation. (2017)
  • Author(s): Nakamura M, Kumrungsee T, Sakuma T, Yamamoto T, Yanaka N.
  • Journal Title: Biosci Biotechnol Biochem. Volume: 81 Issue: 11 Pages: 2164-2167
  • DOI: 10.1080/09168451.2017.1373593
  • Peer Reviewed
• [Journal Article] Magic wands of CRISPR-lots of choices for gene knock-in (2017)
  • Author(s): Sakuma T, Yamamoto T.
  • Journal Title: Cell Biol Toxicol Volume: 33 Issue: 6 Pages: 501-505
  • DOI: 10.1007/s10565-017-9409-6
  • Peer Reviewed
• [Journal Article] PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells (2017)
  • Author(s): Kaku Y, Taguchi A, Tanigawa S, Haque F, Sakuma T, Yamamoto T, Nishinakamura R.
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 4554-4554
  • DOI: 10.1038/s41598-017-04813-3
  • Peer Reviewed / Open Access
• [Journal Article] Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant (2017)
  • Author(s): Mizutani, O., Arazoe, T., Toshida, K., Hayashi, R., Ohsato, S., Skuma, T., Yamamoto, T., Kuwata, S. and Yamada, O.
  • Journal Title: Journal of Biotechnology and Bioengineering Volume: 3 Issue: 3 Pages: 287-293
  • DOI: 10.1016/j.jbiosc.2016.09.014
  • NAID: 40021152739
  • Peer Reviewed
• [Journal Article] Cpf1 and C2c1/2/3―What's next? (2017)
  • Author(s): Shota Nakade, Takashi Yamamoto, Tetsushi Sakuma.
  • Journal Title: Bioengineered Volume: - Issue: 3 Pages: 265-273
  • DOI: 10.1080/21655979.2017.1282018
  • Peer Reviewed / Open Access
• [Journal Article] Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells. (2017)
  • Author(s): Yoshida K, Hozumi A, Treen N, Sakuma T, Yamamoto T, Shirae-Kurabayashi M, Sasakura Y.
  • Journal Title: Developmental Biology Volume: 423 Issue: 2 Pages: 111-125
  • DOI: 10.1016/j.ydbio.2017.01.022
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hox-mediated endodermal identity patterns the pharyngeal muscle formation in the chordate pharynx (2017)
  • Author(s): Yoshida K, Nakahata A, Treen N, Sakuma T, Yamamoto T, Sasakura Y
  • Journal Title: Development Volume: - Pages: 1629-1634
  • DOI: 10.1242/dev.144436
  • Peer Reviewed
• [Journal Article] Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN systems (2017)
  • Author(s): Takayama K., Igai K., Hagihara Y., Hashimoto R., Hanawa M., Sakuma T., Tachibana M., Sakurai F., Yamamoto T., Mizuguchi H
  • Journal Title: Nucleic Acids Research Volume: 45 Issue: 9 Pages: 5198-5207
  • DOI: 10.1093/nar/gkx130
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO (2017)
  • Author(s): Nakamae K, Nishimura Y, Takenaga M, Nakade S, Sakamoto N, Ide H, Sakuma T, Yamamoto T
  • Journal Title: Bioengineered Volume: - Issue: 3 Pages: 302-308
  • DOI: 10.1080/21655979.2017.1313645
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice (2017)
  • Author(s): Nakagawa Y, Sakuma T, Nishimichi N, Yokosaki Y, Takeo T, Nakagata N, Yamamoto T
  • Journal Title: Biology Open Volume: - Pages: 706-713
  • DOI: 10.1242/bio.025122
  • Peer Reviewed / Open Access
• [Journal Article] Systematic cellular disease models reveal synergistic interactions of trisomy 21 and GATA1 mutations in hematopoietic abnormalities (2016)
  • Author(s): K. Banno, S. Omori, K. Hirata, N. Nawa, N. Nakagawa, K. Nishimura, M. Ohtaka, M. Nakanishi, T. Sakuma, T. Yamamoto, T. Toki, E. Ito, T. Yamamoto, C. Kokubu, J. Takeda, H. Taniguchi, H. Arahori, K. Wada, Y. Kitabatake and K. Ozono
  • Journal Title: Cell Reports Volume: 15 Issue: 6 Pages: 1-15
  • DOI: 10.1016/j.celrep.2016.04.031
  • NAID: 120007135439
  • Peer Reviewed / Open Access
• [Journal Article] Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency (2016)
  • Author(s): Nii T, Kohara H, Marumoto T, Sakuma T, Yamamoto T, Tani K
  • Journal Title: Bioresearch Open Access Volume: 5 Issue: 1 Pages: 127-136
  • DOI: 10.1089/biores.2016.0009
  • Peer Reviewed / Open Access
• [Journal Article] Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa (2016)
  • Author(s): Sasaki T, Hanisch FG, Deutzmann R, Sakai LY, Sakuma T, Miyamoto T, Yamamoto T, Hannappel E, Chu ML, Lanig H, von der Mark K
  • Journal Title: Matrix Biol Volume: 56 Pages: 132-149
  • DOI: 10.1016/j.matbio.2016.06.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing. (2016)
  • Author(s): Sato K, Oiwa R, Kumita W, Henry R, Sakuma T, Ito R, Nozu R, Inoue T, Katano I, Sato K, Okahara N, Okahara J, Shimizu Y, Yamamoto M, Hanazawa K, Kawakami T, Kametani Y, Suzuki R, Takahashi T, Weinstein EJ, Yamamoto T, Sakakibara Y, Habu S, Hata J, Okano H, Sasaki E.
  • Journal Title: Cell Stem Cell Volume: 19 Issue: 1 Pages: 127-138
  • DOI: 10.1016/j.stem.2016.06.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single- amino-acid-substituted, and floxed mice (2016)
  • Author(s): Yoshiko Nakagawa, Tetsushi Sakuma, Norihisa Nishimichi, Yasuyuki Yokosaki, Noriyuki Yanaka, Toru Takeo, Naomi Nakagata and Takashi Yamamoto
  • Journal Title: Biology Open Volume: 5 Issue: 8 Pages: 1142-1148
  • DOI: 10.1242/bio.019349
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus. (2016)
  • Author(s): Sakuma T, Masaki K, Abe-Chayama H, Mochida K, Yamamoto T, Chayama K.
  • Journal Title: Genes Cells. Volume: 21 Issue: 11 Pages: 1253-1262
  • DOI: 10.1111/gtc.12437
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy (2016)
  • Author(s): Tochio,N., Umehara,K., Uewaki,J., Flechsig,H., Kondo,M., Dewa,T., Sakuma,T., Yamamoto,T., Saito,T., Togashi,T., Tate,S.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 37887-37887
  • DOI: 10.1038/srep37887
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection (2016)
  • Author(s): Toyonaga K, Torigoe S, Motomura Y, Kamichi T, Hayashi JM, Morita YS, Noguchi N, Chuma Y, Kiyohara H, Matsuo K, Tanaka H, Nakagawa Y, Sakuma T, Ohmuraya M, Yamamoto T, Umemura M, Matsuzaki G, Yoshikai Y, Yano I, Miyamoto T, Yamasaki S.
  • Journal Title: Immunity Volume: 45 Issue: 6 Pages: 1245-1257
  • DOI: 10.1016/j.immuni.2016.10.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ. (2016)
  • Author(s): Aida T, Nakade S, Sakuma T, Izu Y, Oishi A, Mochida K, Ishikubo H, Usami T, Aizawa H, Yamamoto T, Tanaka K.
  • Journal Title: BMC Genomics Volume: 17 Issue: 1 Pages: 979-979
  • DOI: 10.1186/s12864-016-3331-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transcriptional regulation of a horizontally transferred gene from bacterium to chordate. (2016)
  • Author(s): Sasakura Y, Ogura Y, Treen N, Yokomori R, Park SJ, Nakai K, Saiga H, Sakuma T, Yamamoto T, Fujiwara S, Yoshida K.
  • Journal Title: Proc Biol Sci. Volume: 283 Issue: 1845 Pages: 20161712-20161712
  • DOI: 10.1098/rspb.2016.1712
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Parallel generation of multiplex knock-in cell collections using CRISPR-Cas9 assisted by locally enhanced MMEJ (2018)
  • Author(s): Tetsushi Sakuma, Shota Nakade, Keiji Mochida, Kazuki Nakamae, Tomomi Aida, Kohichi Tanaka, Naoaki Sakamoto, and Takashi Yamamoto
  • Organizer: The 9th Takeda Science Foundation Symposium on PharmaSciences
  • Int'l Joint Research
• [Presentation] Biased genome editing using the LoAD (local accumulation of DSB repair molecules) system (2018)
  • Author(s): Tetsushi Sakuma, Shota Nakade, Keiji Mochida, Kazuki Nakamae, Tomomi Aida, Kohichi Tanaka, Naoaki Sakamoto, and Takashi Yamamoto
  • Organizer: Precision Genome Editing with Programmable Nucleases - Keystone Symposia
  • Int'l Joint Research
• [Presentation] Highly practical gene knock-in in mammalian cells and zygotes with MMEJ-dependent strategy (2017)
  • Author(s): Tetsushi Sakuma
  • Organizer: 2nd Annual Genome Editing & Engineering Conference
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2017-02-06
  • Int'l Joint Research / Invited
• [Presentation] Advanced method to generate gene cassette knock-in and floxed mice using the PITCh system facilitated with MMEJ enhancer (2017)
  • Author(s): Tetsushi Sakuma, Tomomi Aida, Shota Nakade, Takashi Yamamoto, Kohichi Tanaka
  • Organizer: Keystone Symposia, Precision Genome Engineering
  • Place of Presentation: Breckenridge, CO, USA
  • Year and Date: 2017-01-08
  • Int'l Joint Research
• [Presentation] Updated summary of genome editing technology (2017)
  • Author(s): Tetsushi Sakuma
  • Organizer: 11th International Symposium on Nanomedicine (ISNM2017)
  • Int'l Joint Research / Invited
• [Presentation] がん抑制およびがんモデリングのための人工転写調節技術の開発 (2017)
  • Author(s): 佐久間 哲史
  • Organizer: 第２回日本遺伝子細胞治療学会若手研究会セミナー
• [Presentation] Recent advances in gene knock-in techniques for cultured cell engineering (2017)
  • Author(s): Tetsushi Sakuma, Shota Nakade, and Takashi Yamamoto
  • Organizer: 2017年度生命科学系学会合同年次大会 (ConBio2017)
• [Presentation] Recent advances in genome editing technology (2017)
  • Author(s): Tetsushi Sakuma
  • Organizer: The 23rd Annual Meeting of Japan Society of Gene and Cell Therapy (JSGCT2017)
  • Invited
• [Presentation] ゲノム編集の技術開発と培養細胞での利用 (2017)
  • Author(s): 佐久間 哲史
  • Organizer: 日本ゲノム編集学会第２回大会
• [Presentation] Cas9 RNP導入細胞のクローン化に伴うheterogeneityの解析 (2017)
  • Author(s): 佐久間 哲史、持田 圭次、中出 翔太、江連 徹、皆川 吉、山本 卓
  • Organizer: 日本ゲノム編集学会第２回大会
• [Presentation] CRISPR-Cas9によるゲノム編集とヒト疾患解析・治療への応用 (2017)
  • Author(s): 佐久間 哲史
  • Organizer: 第57回日本呼吸器学会学術講演会
  • Invited
• [Presentation] Recent Development and Application of Genome Editing Tools and Methods (2017)
  • Author(s): Tetsushi Sakuma
  • Organizer: The 20th US-Japan Cellular and Gene Therapy Conference, CRISPR/Cas9 Gene Editing In Vivo
  • Place of Presentation: Silver Spring, MD, USA
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集の最新技術 (2016)
  • Author(s): 佐久間哲史
  • Organizer: Skeletal Science Retreat 骨代謝スクール
  • Place of Presentation: 熱海
  • Year and Date: 2016-11-26
  • Invited
• [Presentation] Current advances and future prospects of genome editing technology (2016)
  • Author(s): Tetsushi Sakuma
  • Organizer: 10th International Symposium on Nanomedicine (ISNM2016)
  • Place of Presentation: つくば
  • Year and Date: 2016-11-24
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集の最新方法論 (2016)
  • Author(s): 佐久間哲史
  • Organizer: DNA鑑定学会第9回大会
  • Place of Presentation: 東京
  • Year and Date: 2016-11-10
  • Invited
• [Presentation] ゲノム編集の最新方法論 (2016)
  • Author(s): 佐久間哲史
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: 富山
  • Year and Date: 2016-09-28
  • Invited
• [Presentation] ゲノム編集の基礎と最新動向 (2016)
  • Author(s): 佐久間哲史
  • Organizer: 日本ゲノム編集学会第1回大会
  • Place of Presentation: 広島
  • Year and Date: 2016-09-06
• [Presentation] ゲノム編集による遺伝子改変の最前線 (2016)
  • Author(s): 佐久間哲史
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-08-24
  • Invited
• [Presentation] Highly practical gene cassette knock-in in mammalian cells and zygotes mediated by MMEJ (2016)
  • Author(s): Tetsushi Sakuma, Tomomi Aida, Shota Nakade, Kohichi Tanaka, Takashi Yamamoto
  • Organizer: Cold Spring Harbor Laboratory Meeting, Genome Engineering: The CRISPR-Cas Revolution
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2016-08-17
  • Int'l Joint Research
• [Presentation] MMEJ-directed gene cassette knock-in in mammalian cells and zygotes (2016)
  • Author(s): Tetsushi Sakuma, Shota Nakade, Tomomi Aida, Kohichi Tanaka, Takashi Yamamoto
  • Organizer: FASEB Science Research Conference, Genome Engineering - Cutting-Edge Research and Applications
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2016-06-05
  • Int'l Joint Research
• [Book] RNAi and Small Regulatory RNAs in Stem Cells - Methods in Molecular Biology (2017)
  • Author(s): Takata N, Sakakura E, Sakuma T, Yamamoto T.
  • Total Pages: 24
  • Publisher: Springer
  • ISBN: 9781493971060
• [Book] Genome Editing in Animals - Methods in Molecular Biology (2017)
  • Author(s): Sakuma T, Yamamoto T.
  • Total Pages: 12
  • Publisher: Springer
  • ISBN: 9781493971275
• [Book] In Vitro Mutagenesis, Methods in Molecular Biology (2017)
  • Author(s): Sakuma T, Sakamoto T, Yamamoto T
  • Total Pages: 16
  • Publisher: Springer
• [Remarks] 広島大学 分子遺伝学研究室
  • URL: http://www.mls.sci.hiroshima-u.ac.jp/smg/index.html
• [Patent(Industrial Property Rights)] 標的遺伝子にエフェクタータンパク質を集積するための組成物、およびその利用 (2018)
  • Inventor(s): 山本 卓、佐久間 哲史、國井 厚志
  • Industrial Property Rights Holder: 国立大学法人広島大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-041322
  • Filing Date: 2018