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Rapid assay of fecal estrogen in orangutans using near infrared spectroscopy

Research Project

Project/Area Number 16K18482
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Conservation of biological resources
Research InstitutionKyoto University

Principal Investigator

KINOSHITA Kodzue  京都大学, 野生動物研究センター, 助教 (50724233)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords迅速アッセイ / 繁殖 / 野生動物 / ステロイドホルモン / 内分泌 / 排泄物 / 生理モニタリング / イムノクロマト / ストレス / 繁殖モニタリング / 性ホルモン / 近赤外分光法 / 繁殖生理 / モニタリング
Outline of Final Research Achievements

To breed captive animals succesfully, it is important to determine the optimal timing for breeding. In this study, we focused on Borneo orangutan (Pongo pygmaeus) and conducted research to establish a method to rapidy and easily identify the optimal timing for breeding in captivity and the wild. In addition, by using excrement for analysis, we established methods that can be used non-invasively / indirectly on animals. Finally, application of immunochromatography in addition to near-infrared spectroscopy has led to the establishment of a simpler and more rapid hormone analysis method.

Academic Significance and Societal Importance of the Research Achievements

本手法はオランウータンだけでなく、動物園で飼育されている数多くの動物の日々のホルモン検査に役立つことが期待される。近赤外分光法およびイムノクロマト法は、いずれも小型機器での分析が可能であり、簡便に操作できる。そのため、飼育下では展示場など屋外での測定も可能となり、飼育係などの非専門家でも日々の測定が容易くなる。また、屋外でホルモン検査が可能とるため、飼育下だけでなく野生個体にも応用可能であり、野生個体のホルモン動態や繁殖状況の把握など、多くの研究分野への展開が期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (37 results)

All 2019 2018 2017 2016 Other

All Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Open Access: 3 results) Presentation (27 results) (of which Int'l Joint Research: 11 results,  Invited: 2 results) Remarks (2 results)

  • [Journal Article] A Review of Hormone Analysis on Primate Ecology2018

    • Author(s)
      木下こづえ
    • Journal Title

      Primate Research

      Volume: 34 Issue: 1 Pages: 5-15

    • DOI

      10.2354/psj.34.008

    • NAID

      130007437787

    • ISSN
      0912-4047, 1880-2117
    • Year and Date
      2018-06-20
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 520 in Altmetrics). 2.Takeshita RSC, Bercovitch FB, Huffman MA, Kinoshita K (2018). Development and validation of an enzyme immunoassay for fecal dehydroepiandrosterone-sulfate (fDHEAS) in Japanese macaques (Macaca fuscata)2018

    • Author(s)
      Takeshita RSC, Bercovitch FB, Huffman MA, Kinoshita K
    • Journal Title

      International Journal of Primatology

      Volume: Online version Issue: 2 Pages: 1-14

    • DOI

      10.1007/s10764-018-0026-x

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Beneficial effect of hot spring bathing on stress levels in Japanese macaques2018

    • Author(s)
      Takeshita RSC, Bercovitch FB, Kinoshita K, Huffman MA
    • Journal Title

      Primates

      Volume: 59 Issue: 3 Pages: 215-225

    • DOI

      10.1007/s10329-018-0655-x

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of castration on social behavior and hormones in male Japanese macaques (Macaca fuscata).2017

    • Author(s)
      Takeshita RSC, Huffman MA, Kinoshita K, Bercovitch FB.
    • Journal Title

      Physiology & Behavior

      Volume: 181 Pages: 43-50

    • DOI

      10.1016/j.physbeh.2017.09.006

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of urinary estrogen conjugates and creatinine using near infrared spectroscopy in Bornean orangutans (Pongo Pygmaeus).2016

    • Author(s)
      Kinoshita K, Kuze N, Kobayashi T, Miyakawa E, Narita H, Inoue-Murayama M, Idani G,Tsenkova R
    • Journal Title

      Primates

      Volume: 57 Issue: 1 Pages: 51-59

    • DOI

      10.1007/s10329-015-0501-3

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis of hair cortisol levels in captive chimpanzees: effect of various methods on cortisol stability and variability2016

    • Author(s)
      Yamanashi, Y., Teramoto, M., Morimura, N., Hirata, S., Suzuki, J., Hayashi, M., Kinoshita, K., Murayama, M., Idani, G.
    • Journal Title

      MethodsX

      Volume: 3 Pages: 110-117

    • DOI

      10.1016/j.mex.2016.01.004

    • NAID

      120005820907

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Behavioral and physiological changes in a juvenile Bornean orangutan after a wildlife rescue2016

    • Author(s)
      Mendonca RS. , Takeshita RSC. , Kanamori T, Kuze N, Hayashi M, Kinoshita K, Bernard H, Matsuzawa T
    • Journal Title

      Global Ecology and Conservation

      Volume: 8 Pages: 116-122

    • DOI

      10.1016/j.gecco.2016.08.004

    • NAID

      120006223581

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Urinary sex steroid hormone and placental leucine aminopeptidase concentration differences between live births and stillbirth of Bornean orangutans (Pongo pygmaeus).2016

    • Author(s)
      Kinoshita K, Sano Y, Takai A, Shimizu M, Kobayashi T, Ouchi A, Kuze N, Inoue-Murayama M, Idani G, Okamoto M, Ozaki Y
    • Journal Title

      Journal of Medical Primatology

      Volume: 46 Issue: 1 Pages: 3-8

    • DOI

      10.1111/jmp.12249

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Development of a field-friendly method for steroid hormone analysis of wild animals2019

    • Author(s)
      Kinoshita K
    • Organizer
      Symposium of Intergrative Biology -Biodiversity in Asia-
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Field-friendly法を用いた野生動物の迅速ホルモン分析手法の開発2019

    • Author(s)
      木下こづえ, 菊地デイル万次郎,大西敏文,秋山多江,Suraiya Leuche, Koustubh Sharma, Kubanchbek Zhumabai uulu
    • Organizer
      第66回日本生態学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 3-D camera trapping: a novel method for morphometric and locomotive measurements in snow leopards (Panthera uncia)2018

    • Author(s)
      Kikuchi DM, Kinoshita K, Zhumabai uulu K, Sharma K
    • Organizer
      Conservation Asia 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Challenge of conservative physiology in snow leopards with collaboration of noninvasive methods2018

    • Author(s)
      Kinoshita K, Kikuchi DM, Luecke S, Zhumabai uulu K, Sharma K
    • Organizer
      Conservation Asia 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Kinoshita K. Challenge of conservative physiology in snow leopards: from captive to wild animals2018

    • Author(s)
      Kinoshita K
    • Organizer
      The 7th International Workshop on Tropical Biodiversity Conservation Focusing on Large Animal Studies
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] he relationship between pregnancy of wild Borneo Orangutan and fruit availability2018

    • Author(s)
      Kuze N, Kanamori T, Mendonca R, Yamazaki S, Tajima T, Tsutaya T, Bernard H, Malim P, Kinoshita K, Kohshima S
    • Organizer
      The 7th International Workshop on Tropical Biodiversity Conservation Focusing on Large Animal Studies
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 排泄物から動物の“こころ”を知りたい:動物園動物から野生動物まで2018

    • Author(s)
      木下こづえ
    • Organizer
      関西実験動物研究会第140回研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 飼育下ハヌマンラングールのメスにおける糞中性ホルモン濃度動態調査2018

    • Author(s)
      木村嘉孝,川出比香里,土屋知己,川本奈緒,木下こづえ
    • Organizer
      第66回飼育技術者研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 野生ボルネオ・オランウータン(Pongo pygmaeusmorio)の雌の妊娠と一斉結実との関係-13年間の記録-2018

    • Author(s)
      久世濃子,金森朝子,山崎彩夏,田島知之,蔦谷 匠,Renata Mendoça, Henry Bernard,Peter T. Malim,木下こづえ
    • Organizer
      日本哺乳類学会2018年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 飼育下コアラ(Phasocolarctos cinereus)における尿中ステロイドホルモン濃度測定による生理評価2018

    • Author(s)
      宮川悦子,柴田枝梨,矢口 茜,木下こづえ
    • Organizer
      第66回飼育技術者研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] To carry or not to carry: changes in stress hormones in a case of dead infant carrying in Japanese macaques.2018

    • Author(s)
      Takeshita RS, Huffman MA, Kinoshita K, Bercovitch FB.
    • Organizer
      The 9th Annual Symposium of Leading Graduate Program in Primatology and Wildlife Science
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 雌オランウータンにおける生殖器の状態と尿中性ステロイドホルモン濃度の関係性について2017

    • Author(s)
      木下こづえ,中村智行,久世濃子,尾崎康彦
    • Organizer
      「ず~ぞなもし。」動物園大学7 inとべ
    • Place of Presentation
      伊予
    • Year and Date
      2017-03-20
    • Related Report
      2016 Research-status Report
  • [Presentation] 飼育下チンパンジーの授乳期の性皮腫脹について2017

    • Author(s)
      奥村文彦,廣澤麻里,藤森唯,星野智紀,坂口真悟,綿貫宏史朗,木下こづえ,岡部直樹,木村直人,伊谷原一
    • Organizer
      第64回動物園技術者研究会
    • Place of Presentation
      犬山
    • Year and Date
      2017-02-14
    • Related Report
      2016 Research-status Report
  • [Presentation] Endocrinological characteristic of Yaku sika deer.2017

    • Author(s)
      Kinoshita K, Agetsuma-Yanagihara Y, Agetsuma N, Sugiura H, Hanya G, Hayakawa T, Yanagawa Y.
    • Organizer
      The 7th International Seminar on Biodiversity and Evolution: Genetic and Hormonal Analyses on Wild Animals
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Application possibility of non-invasive hormonal analysis for the estimation of wildlife physiological state.2017

    • Author(s)
      Kinoshita K.
    • Organizer
      The 6th International Symposium on Tropical Biodiversity in Large
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヤクシカにおける糞中性ステロイドホルモン濃度の季節変化―エゾシカと比較して―.2017

    • Author(s)
      木下こづえ,揚妻‐柳原芳美,揚妻直樹,杉浦秀樹,半谷吾郎,早川卓志,栁川洋二郎.
    • Organizer
      第23回野生動物医学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 排泄物中コルチゾール濃度測定を用いた飼育下コアラ(Phascolarctos cinereus)のストレスモニタリングの検討.2017

    • Author(s)
      宮川悦子,柴田枝梨,矢口 茜,木下こづえ.
    • Organizer
      第23回野生動物医学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ホルモン分析を用いた生理生態研究.2017

    • Author(s)
      木下こづえ.
    • Organizer
      第33回日本霊長類学会
    • Related Report
      2017 Research-status Report
  • [Presentation] レオとともに歩んだ10年2016

    • Author(s)
      兼子明久,林 美里,櫻庭陽子,宮部貴子,前田典彦,山中淳史,ゴドジャリ静,木下こづえ,友永雅己
    • Organizer
      Support for African/Asian great Apes (SAGA) 19
    • Place of Presentation
      宇部
    • Year and Date
      2016-11-19
    • Related Report
      2016 Research-status Report
  • [Presentation] ボルネオオランウータン(Pongo Pygmaeus)の精液性状とその液状保存に関する研究2016

    • Author(s)
      木下こづえ,久世濃子,宮川悦子,小林智男,中村智行,尾形光昭,尾崎康彦
    • Organizer
      第22回日本野生動物医学会大会
    • Place of Presentation
      宮崎
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 室内飼育下の雌ニホンザル(Macaca fuscata)における性周期の特徴2016

    • Author(s)
      佐藤 容,印藤頼子,木下こづえ,柳川洋二郎,外丸祐介,岡本宗裕
    • Organizer
      第22回日本野生動物医学会大会
    • Place of Presentation
      宮崎
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Reproductive monitoring of captive Bornean orangutans (Pongo pugmaeus) in Japanese zoos2016

    • Author(s)
      Kinoshita K
    • Organizer
      2016 Wildlife Medicine Clinical Technique Training Course
    • Place of Presentation
      Kota Kinabalu
    • Year and Date
      2016-09-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Liquid storage of captive Bornean orangutan (Pongo Pygmaeus) sperm collected without anesthesia2016

    • Author(s)
      Kinoshita K, Kuze K, Miyakawa E, Kobayashi T, Nakamura T, Ogata M, Ozaki Y
    • Organizer
      Joint meeting of the International Primatological Society and the American Society of Primatologists
    • Place of Presentation
      Chicago
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The effect of castration and the environment on the relationship between dominance rank and fecal steroid concentrations in male Japanese macaques (Macaca Fuscata)2016

    • Author(s)
      Takeshita RS, Huffman MA, Kinoshita K, Bercovitch FB
    • Organizer
      Joint meeting of the International Primatological Society and the American Society of Primatologists
    • Place of Presentation
      Chicago
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 飼育下チンパンジー(Pan troglodytes)の授乳期における発情回帰と性ステロイドホルモン濃度動態との関連性について2016

    • Author(s)
      木下こづえ,奥村文彦,星野智紀,廣澤麻里,坂口真悟,綿貫宏史朗,岡部直樹,木村直人,伊谷原一,鵜殿敏史,市野悦子,高島友子,林 美里,岡本宗裕
    • Organizer
      第32回日本霊長類学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2016-07-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 室内個別ケージ飼育下における雌ニホンザル(Macaca fuscata)の尿中性ステロイドホルモン濃度について2016

    • Author(s)
      佐藤 容,印藤頼子,木下こづえ,柳川洋二郎,外丸祐介,岡本宗裕
    • Organizer
      第32回日本霊長類学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2016-07-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 定期的な超音波検査がニホンザル(Macaca fuscata)の性周期に与える影響2016

    • Author(s)
      印藤頼子,南 晶子,兼子明久,佐藤 容,木下こづえ,柳川洋二郎,永野昌志,岡本宗裕
    • Organizer
      第32回日本霊長類学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2016-07-15
    • Related Report
      2016 Research-status Report
  • [Remarks] 京都大学野生動物研究センター

    • URL

      http://www.wrc.kyoto-u.ac.jp/members/kinoshita.html

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
  • [Remarks] 木下こづえ個人webページ

    • URL

      https://www.kodzue-kinoshita.com/

    • Related Report
      2018 Annual Research Report 2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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