Exploration of quality control mechanism during U snRNA / snRNP biogenesis process
Project/Area Number |
16K18489
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
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Research Institution | Tokyo University of Agriculture and Technology |
Principal Investigator |
Ishikawa Hideaki 東京農工大学, (連合)農学研究科(研究院), 助教 (80625715)
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | RNA / リボヌクレオプロテオミクス / 分子間相互作用 / RNA品質管理機構 / 転写後修飾 |
Outline of Final Research Achievements |
U1-tfs, derivatives of U1 snRNA, are generated presumably by quality control mechanisms in the process of U1 snRNA biogenesis and eliminated through P-body dependent RNA degradation. However, the detailed molecular mechanisms involved in the formation of U1-tfs had not been clarified. To elucidate the mechanisms underlying U1-tfs formation, we first examined the cellular compartments where U1-tfs are generated by using the nuclear export defect mutants of PHAX, which is required for the nuclear export of U snRNAs, and revealed that U1-tfs are formed already in the process between transcription and nuclear export in the nucleus. We therefore examined the involvement of nuclear-localized 3’-5’ exoribonucleases in the formation of U1-tfs and found that exoribonuclease ERI1 might play a role in the U1-tfs formation by targeting U1 snRNA species which are failed to be processed by TOE1, the responsible enzymes for normal U snRNAs 3’end trimming.
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Report
(3 results)
Research Products
(4 results)
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[Journal Article] TDP-43 stabilises the processing intermediates of mitochondrial transcripts.2017
Author(s)
Izumikawa K, Nobe Y, Yoshikawa H, Ishikawa H, Miura Y, Nakayama H, Nonaka T, Hasegawa M, Egawa N, Inoue H, Nishikawa K, Yamano K, Simpson RJ, Taoka M, Yamauchi Y, Isobe T, Takahashi N
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Journal Title
Sci Rep
Volume: 7
Issue: 1
Pages: 7709-7709
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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