• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

D-amino acid biological system in eukaryote

Research Project

Project/Area Number 16K18686
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionNagoya University

Principal Investigator

Ito Tomokazu  名古屋大学, 生命農学研究科, 講師 (90584970)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsD-セリン / D-アスパラギン酸 / D-アミノ酸 / D-セリンデヒドラターゼ / バイオマーカー / セリンラセマーゼ / 腎機能
Outline of Final Research Achievements

We analyzed the catalytic and regulatory mechanisms of D-Asp synthetic activity of mammalian serine racemase (SR). Data inidicaed that SR is one of mammalian D-Asp biosynthetic enzymes. In addition, we analyzed urinary D-Ser using our D-, and L-Ser enzymatic assay method. The results obtained here suggested that D-Ser levels could be used as a predictable biomarker of renal function reduction in some situations. In addition, we studied the molecular mechanism of developmental abnormalities of Dsd-deficient Dicsyotelium strains and revealed the involvment of D-Ser in cAMP signaling. We found a novel Dsd substrate D-amino acid in the fruiting body of Bunashimeji mushroom, and identified it as (2R, 3S) 2-amino-3,4-dihydroxybutanoic acid.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Production of Ophthalmic Acid Using Engineered Escherichia coli2018

    • Author(s)
      Ito T, Tokoro M, Hori R, Hemmi H, Yoshimura T
    • Journal Title

      Appl Environ Microbiol

      Volume: 84 Issue: 7 Pages: 02806-02817

    • DOI

      10.1128/aem.02806-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Occurrence of the (2R,3S)-Isomer of 2-Amino-3,4-dihydroxybutanoic Acid in the Mushroom Hypsizygus marmoreus.2017

    • Author(s)
      Ito T, Yu Z, Yoshino I, Hirozawa Y, Yamamoto K, Shinoda K, Watanabe A, Hemmi H, Asada Y, Yoshimura T.
    • Journal Title

      J Agric Food Chem.

      Volume: 65 Issue: 30 Pages: 6131-6139

    • DOI

      10.1021/acs.jafc.7b01893

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Serine racemase is involved in D-aspartate biosynthesis.2016

    • Author(s)
      Ito, T., Hayashida, M., Kobayashi, S., Muto, N., Hayashi, A., Yoshimura, T., Mori, H.
    • Journal Title

      J Biochem.

      Volume: 160 Issue: 6 Pages: 345-353

    • DOI

      10.1093/jb/mvw043

    • NAID

      40021050460

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] D-アミノ酸合成酵素のファンクショナルクローニング系の開発2018

    • Author(s)
      武藤 菜摘、伊藤 智和、吉村 徹、邊見 久
    • Organizer
      2018年度日本農芸化学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] D-セリンデヒドラターゼの基質となるヒト尿中未知アミノ酸の同定2018

    • Author(s)
      戸野真由香、伊藤智和、邊見久、吉村徹
    • Organizer
      2018年度日本農芸化学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] E. coliビタミンB6結合タンパク質YggSの機能解明:glyAとの合成致死性の解析2018

    • Author(s)
      堀 蘭、伊藤 智和、山内 綾子、吉村 徹、邊見 久
    • Organizer
      2018年度日本農芸化学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ブナシメジに存在する新奇D-アミノ酸の同定2017

    • Author(s)
      伊藤智和・余卓尓・吉野一晴・渡邊彰・邊見久・麻田恭彦・吉村徹
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] E.coliのPLP結合タンパク質YggSの生理機能:欠損株が示すホモセリン毒性の要因の解析2017

    • Author(s)
      伊藤 智和、堀 蘭、山内 綾子、吉村 徹、邊見 久
    • Organizer
      2017酵素・補酵素研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] D-セリンデヒドラターゼの基質となるヒト尿中未知アミノ酸の同定2017

    • Author(s)
      戸野真由香、伊藤智和、邊見久、吉村徹
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高度に保存された機能未知タンパク質の機能解析2017

    • Author(s)
      伊藤 智和、吉村 徹
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Unusual D-Amino acids in mushroom2017

    • Author(s)
      Tomokazu Ito, Zhuoer Yu, Issei Yoshino, Yurina Hirozawa, Kana Yamamoto, Akira Watanabe, Hisashi Hemmi, Yasuhiko Asada, Tohru Yoshimura
    • Organizer
      International Conference of D-Amino Acid Research(IDAR2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] D-アミノ酸研究における代謝酵素の応用利用2017

    • Author(s)
      伊藤 智和
    • Organizer
      D-アミノ酸学会第三回ワークショップ
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ブナシメジに存在するユニークなD-アミノ酸の同定と生長過程における動態解析2016

    • Author(s)
      伊藤智和・余卓尓・吉野一晴・渡邊彰・邊見久・麻田恭彦・吉村徹
    • Organizer
      D-アミノ酸学会
    • Place of Presentation
      高知大学
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 哺乳類におけるD-アスパラギンサンの生合成機構と抗酸化作用の解析2016

    • Author(s)
      武藤菜摘・伊藤智和・邊見久・川原幸一・吉村徹
    • Organizer
      日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      三重大学
    • Year and Date
      2016-05-21
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi