| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
|---|
| Outline of Final Research Achievements |
We identified the pathogenic kappa light chain causing crystal-storing histiocytosis and succeeded in producing huge amount of the protein. We confirmed that this pathogenic kappa light chain was endocytosed and crystalized in mice macrophages, podocytes, and proximal tubular cells in vitro. However, we did not determine how the light chain has its toxicity for those cells and the mechanism of kappa light chain integration into those cells. We created transgenic mice which secrete the pathogenic kappa light chain. The transgenic mice formed macrophages containing kappa crystals in spleen, but did not show any kidney involvements of crystal-storing histiocytosis due to small amount of pathogenic kappa light chain secretion.
|
