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Vaccine development utilizing gnotobiotic pig model for controlling viral gastroenteritis(Fostering Joint International Research)

Research Project

Project/Area Number 16KK0178
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Hygiene and public health
Research InstitutionThe University of Tokyo

Principal Investigator

Takanashi Sayaka  東京大学, 大学院医学系研究科(医学部), 助教 (20645709)

Project Period (FY) 2017 – 2019
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Keywords胃腸炎 / Gnotobiotic pig model / ロタウイルス / ワクチン / 栄養 / 腸内細菌叢 / ウイルス性胃腸炎 / Gnotobiotic pigs
Outline of Final Research Achievements

By using malnourished-human infant fecal microbiota transplanted neonatal pig models, we demonstrated that protein malnutrition hampered the efficacy of an oral attenuated human rotavirus vaccine by altering adaptive and innate immune responses.
Our findings on lower level of IL-22 and higher amount of LPS in protein-deficient diet pigs would provide novel insights on intestinal membrane regeneration and possible interactions among nutrition, microbiota and host immunity.

Academic Significance and Societal Importance of the Research Achievements

研究代表者が博士研究員として留学したネットワークを活かし、オハイオ州立大学で長年実績のあるgnotobiotic pigを用いて、日本で近々定期接種化が計画されているHRVワクチンの国際共同研究を行った。ヒト乳児由来糞便細菌叢移植gnotobiotic pigsの系は、腸内細菌叢、栄養と免疫との相互作用解明に有用な動物モデルであり、HRV感染症対策には、ワクチンの活用に加えて、衛生や栄養も考慮した包括的な取り組みが必要なことが示唆された。

Report

(2 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (20 results)

All 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (10 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Int'l Joint Research] The Ohio State University(米国)2018

    • Year and Date
      2018-03-10
    • Related Report
      2019 Annual Research Report
  • [Journal Article] Distribution of rotavirus genotypes in Japan from 2015 to 2018: Diversity in genotypes before and after introduction of rotavirus vaccines.2020

    • Author(s)
      Hoque SA, Khandoker N, Thongprachum A, Khamrin P, Takanashi S, Okitsu S,
    • Journal Title

      Vaccine

      Volume: 印刷中 Issue: 23 Pages: 3980-3986

    • DOI

      10.1016/j.vaccine.2020.03.061

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ロタウイルス胃腸炎とMicrobiome2020

    • Author(s)
      高梨 さやか
    • Journal Title

      臨床とウイルス

      Volume: 48 Pages: 45-51

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Comparative Sequence Analysis of Historic and Current Porcine Rotavirus C Strains and Their Pathogenesis in 3-Day-Old and 3-Week-Old Piglets2020

    • Author(s)
      Juliet Chepngeno, Sayaka Takanashi, Annika Diaz, Husheem Michael, Francine C Paim, Michael C Rahe, Jeffrey R Hayes, Courtney Baker, Douglas Marthaler, Linda J Saif, Anastasia N Vlasova
    • Journal Title

      Front Microbiol

      Volume: 印刷中

    • DOI

      10.3389/fmicb.2020.00780

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Determining effectiveness of rotavirus vaccine by immunochromatography and reverse transcriptase polymerase chain reaction: A comparison.2019

    • Author(s)
      Hoque SA, Iizuka I, Kobayashi M, Takanashi S, Anwar KS, Islam MT, Hoque SA, Khamrin P, Okitsu S, Hayakawa S, Ushijima H.
    • Journal Title

      Vaccine

      Volume: 39 Issue: 39 Pages: 5886-5890

    • DOI

      10.1016/j.vaccine.2019.07.091

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular detection of enteric viruses in the stool samples of children without diarrhea in Bangladesh.2019

    • Author(s)
      Okitsu S, Khamrin P, Takanashi S, Thongprachum A, Hoque SA, Takeuchi H, Khan MA, Hasan SMT, Iwata T, Shimizu H, Jimba M, Hayakawa S, Maneekarn N, Ushijima H.
    • Journal Title

      Infect Genet Evol

      Volume: 77 Pages: 104055-104055

    • DOI

      10.1016/j.meegid.2019.104055

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Detection of mutations in the VP7 gene of vaccine-derived strains shed by monovalent rotavirus vaccine recipients.2019

    • Author(s)
      Kaneko M, Takanashi S, Inoue M, Sakiyama H, Okitsu S, Mizuguchi M, Ushijima H
    • Journal Title

      Access Microbiology

      Volume: acmi.0.000033 Issue: 8

    • DOI

      10.1099/acmi.0.000033

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] How the gut microbiome regulates host immune responses to viral vaccines.2019

    • Author(s)
      Vlasova AN, Takanashi S, Miyazaki A, Rajashekara G, Saif LJ
    • Journal Title

      Current opinion in virology

      Volume: 37 Pages: 16-25

    • DOI

      10.1016/j.coviro.2019.05.001

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Diagnosis of Acute Gastroenteritis with Immunochromatography and Effectiveness of Rotavirus Vaccine in a Japanese Clinic.2019

    • Author(s)
      Kawata K, Hikita T, Takanashi S, Hikita H, Ogita K, Okitsu S, Hoque SA, Phan T G, Ushijima H.
    • Journal Title

      Access Microbiol.

      Volume: acmi.0.000085. Issue: 3

    • DOI

      10.1099/acmi.0.000085

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Molecular detection of sapovirus in the children with acute gastroenteritis collected from one outpatient clinic in Japan, 2014 to 20172019

    • Author(s)
      Okitsu S, Khamrin P, Hikita T, Takanashi S, Hayakawa S, Maneekarn N, Ushijima H
    • Organizer
      7th International Calicivirus Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Detection of gastroenteritis viruses in environmental rain waters and dusts2019

    • Author(s)
      Ushijima H, Kawata K, Okuda T, Hoque A, Nomura A, Shimizu-Onda Y, Okitsu S, Khamrin P, Takanashi S, Hayakawa S, Maneekarn N
    • Organizer
      7th International Calicivirus Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イムノクロマト法によるロタウイルス抗原血症の検討2019

    • Author(s)
      牛島 廣治、疋田 敏之、Kim Pham、飯塚 愛恵、恩田 優子、川岸 崇裕、金井 裕太、河本聡志、高梨さやか、沖津 祥子、田島 剛、菊田英明、小林 正明、山本 あつ子、西村 修一、杉田 久美子、馬場 常嘉、小林 剛、谷口 孝喜、早川 智
    • Organizer
      第51回日本小児感染症学会総会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イムノクロマト法による胃腸炎の 診断とロタウイルスワクチンの効果についての検討2019

    • Author(s)
      疋田敏之、疋田博之、疋田房子、高梨 さやか、沖津祥子、Hoque Ariful、牛島廣治
    • Organizer
      第51回日本小児感染症学会総会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イムノクロマト法と酵素抗体法によるロタウイルス抗原血症と抗原尿症の検索2019

    • Author(s)
      牛島 廣治、塩田 愛恵、清水 優子、宮下 英将、小林 正明、カムリン パタラ、ホク アリフル、高梨さやか、沖津 祥子、川岸 崇裕、金井 裕太、河本聡、谷口 孝喜、小林 剛、田島 剛、マネカンニワット、早川 智
    • Organizer
      第67回日本ウイルス学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低栄養児由来便細菌叢移植無菌ブタモデルを用いた蛋白質欠乏餌によるヒトロタウイルスワクチン効果減弱の解析2019

    • Author(s)
      高梨 さやか、宮崎 綾子、マイケル フシーム、ペイム フランシン、テャンペンチノ ジュリエット、スリバスタナ ヴィシャール、ラジャセカラ ギリシュ、サイフ リンダ、ブラソバ アナスタシア
    • Organizer
      第67回日本ウイルス学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単価ロタウイルスワクチン培養精製抗原を用いた妊婦血清中抗ロタウイルス抗体価の検討 ワクチン免疫原性に影響を与える母体由来因子の解明を目指して2019

    • Author(s)
      高梨 さやか, 沖津 祥子, 柴村 美帆, 牛島 廣治
    • Organizer
      第60回日本臨床ウイルス学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Molecular Epidemiology of Gastroenteritis Viruses in Pediatric Patients in Japan2019

    • Author(s)
      Khamrin Pattara, 飯塚 愛恵, 沖津 祥子, 高梨 さやか, 早川 智, 牛島 廣治
    • Organizer
      第60回日本臨床ウイルス学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Determination of Effectiveness of Rotavirus Vaccine by Immunochromatography and Reverse Transcriptase Polymerase Chain Reaction2019

    • Author(s)
      Hoque Ariful, 飯塚(塩田) 愛恵, 小林 正明, 高梨 さやか, Khamrin Pattara, 沖津 祥子, 早川 智, 牛島 廣治
    • Organizer
      第60回日本臨床ウイルス学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単価ロタウイルスワクチン培養精製抗原を用いた妊婦血清中抗ロタウイルス抗体価の検討2019

    • Author(s)
      高梨 さやか, 中村 佳恵, 水野 葉子, 名西 恵子, 星野 愛, 安戸 裕貴, 岡 明, 沖津 祥子, 牛島 廣治, 水口 雅
    • Organizer
      第122回日本小児科学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 東京大学大学院医学系研究科発達医科学 ホームページ

    • URL

      http://www.development.m.u-tokyo.ac.jp/

    • Related Report
      2019 Annual Research Report

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Published: 2017-03-15   Modified: 2021-02-19  

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