Epigenetics analysis of HTLV-1 provirus through international comparison(Fostering Joint International Research)
Project/Area Number |
16KK0206
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Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | Kumamoto University |
Principal Investigator |
Katsuya Hiroo 熊本大学, エイズ学研究センター, 特任助教 (80632041)
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Research Collaborator |
Satou Yorifumi 熊本大学, エイズ学研究センター, 教授
Bangham Charles インペリアル大学, 医学部免疫学講座, 教授
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Project Period (FY) |
2016 – 2018
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
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Keywords | HTLV-1 / 成人T細胞白血病・リンパ腫 / レトロウイルス / 転写制御 / DNA-capture-seq / 成人T細胞白血病-リンパ腫 / HTLV-1関連脊髄症 / 転写 / 成人T細胞白血病 / ウィルス / 遺伝子 |
Outline of Final Research Achievements |
We have developed the use of DNA probes for the capture of HTLV-1 fragments from a library prepared for next-generation sequence to efficiently analyze epigenetic features of the provirus. Using this approach, we found the novel enhancer region near the 3’LTR in HTLV-1. This enhancer region might contribute to keep HBZ expression constant, and thus maintain persistent HTLV-1 infection. DNA-capture-seq, furthermore, revealed that infected clones with defective-type provirus exhibited higher degree of clonal abundance than those with full-length type. This finding indicated that defective proviruses, which could not produce viral antigens, should allow the infected cell to escape from the host immune surveillance, resulting in clonal expansion of the infected cells.
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Academic Significance and Societal Importance of the Research Achievements |
HTLV-1の新たな転写制御メカニズムを見出した。ウイルス遺伝子であるHBZの恒常的発現の機序の一つである可能性がある。HBZはHTL-1感染細胞の増殖に関わることが知られており、今後のHTLV-1感染細胞の増殖制御に関わる研究につなげることができる。
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Prognostic index for chronic- and smoldering-type adult T-cell leukemia-lymphoma2017
Author(s)
Katsuya Hiroo、Shimokawa Mototsugu、Ishitsuka Kenji、Kawai Kazuhiro、Amano Masahiro、Utsunomiya Atae、Hino Ryosuke、Hanada Shuichi、Jo Tatsuro、Tsukasaki Kunihiro、Moriuchi Yukiyoshi、Sueoka Eisaburo、Yoshida Shinichiro、Suzushima Hitoshi、Miyahara Masaharu、Yamashita Kiyoshi、Eto Tetsuya、Suzumiya Junji、Tamura Kazuo
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Journal Title
Blood
Volume: 130
Issue: 1
Pages: 39-47
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A prognostic index for chronic- and smoldering-type adult T-cell leukemia-lymphoma2017
Author(s)
Katsuya H, Shimokawa M, Ishitsuka K, Kawai K, Amano M, Utsunomiya A, Hino R, Hanada S, Jo T, Tsukasaki K, Moriuchi Y, Sueoka E, Yoshida S, Suzushima H, Miyahara M, Yamashita K, Eto T, Suzumiya J, Tamura K.
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Journal Title
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Highly sensitive viral RNA-seq is a powerful tool to analyze the viral transcripts in HTLV-1-infected individuals2017
Author(s)
Yuki Inada, Hiroo Katsuya, Paola Miyazato, Benjy Tan Jek Yang, Saori Iwase, Misaki Kakoki, Misaki Matsuo, Islam Mohammad Saiful, Masahito Tokunaga, Hiroyuki Hata, Yoshihisa Yamano, Atae Utsunomiya, Yorifumi Satou
Organizer
The 59th American Society of Hematology
Related Report
Int'l Joint Research
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[Presentation] Possible involvement of YB-1 in abnormal nuclear shape of adult T-cell leukemia-Iymphoma cells2017
Author(s)
Misaki Kakoki, Hiroo Katsuya, Takafumi Noguchi, Yuki Inada, Paola Miyazato, Misaki Matsuo, Benjy Tan Jek Yang, Islam Mohammad Saiful, Hiroyuki Hata, Tokio Tani, Yorifumi Satou
Organizer
第4回日本HTLV-1学会学術集会
Related Report
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[Presentation] Possible involvement of YB-1 in abnormal nuclear shape of adult T-cell leukemia-Iymphoma cells2017
Author(s)
Misaki Kakoki, Hiroo Katsuya, Takafumi Noguchi, Yuki Inada, Paola Miyazato, Misaki Matsuo, Masahito Tokunaga, Atae Utsunomiya, Hiroyuki Hata, Tokio Tani, Yorifumi Satou
Organizer
第79回日本血液学会学術集会
Related Report
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