| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
We developed and implemented algorithms that can characterize large-scale structural changes in large-biomolecular complexes. Then, the methods are applied to biological problems, in this case, nucleosome structural dynamics.
First, we investigated thermally-driven spontaneous and ATP-dependent chromatin remodeler-driven nucleosome sliding processes. For the purpose, utilizing our own coarse-grained molecular dynamics methods, we could perform complete transition pathways analysis via Markov-state-modeling. In addition, we realized free energy landscape of nucleosome self-organization, again via Markov state modeling, from which we obtained detailed and salt-concentration dependent nucleosome formation/disassembly pathways.
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