Mechanisms that lead to the difference in equational and reductional chromosome segregation

• Project/Area Number: 17002014
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: WATANABE Yoshinori 東大, 分子細胞生物学研究所, 教授 (20212326)
• Co-Investigator(Renkei-kenkyūsha): SAKUNO Takeshi 東京大学, 分子細胞生物学研究所, 特任助教 (10504566)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥399,490,000 (Direct Cost: ¥307,300,000、Indirect Cost: ¥92,190,000); Fiscal Year 2009: ¥53,690,000 (Direct Cost: ¥41,300,000、Indirect Cost: ¥12,390,000); Fiscal Year 2008: ¥53,690,000 (Direct Cost: ¥41,300,000、Indirect Cost: ¥12,390,000); Fiscal Year 2007: ¥67,080,000 (Direct Cost: ¥51,600,000、Indirect Cost: ¥15,480,000); Fiscal Year 2006: ¥110,240,000 (Direct Cost: ¥84,800,000、Indirect Cost: ¥25,440,000); Fiscal Year 2005: ¥114,790,000 (Direct Cost: ¥88,300,000、Indirect Cost: ¥26,490,000)
• Keywords: 遺伝学 / ゲノム / シュゴシン / 染色体接着因子 / 体細胞分裂 / 減数分裂 / 染色体接着保護 / 還元分裂 / 動原体 / セントロメア

Research Abstract

体細胞が増殖分裂する過程で、複製された染色体のコピーが娘細胞へ均等に分配されるためには、染色体の中心部分にある動原体が反対方向からのスピンドル微小管によって捕らえられることが重要である。このためには、細胞分裂のときに、動原体部分の接着が維持されていることと、その向きが正しく制御されることが必要である。この過程には、我々が酵母において発見し命名したシュゴシンとMoa1というタンパク質が本質的な役割を持つことが明らかになった。この機構は、ヒトにおいても保存されていると考えられる。

Research Products

• [Journal Article] Studies of meiosis disclose distinct roles of cohesion in the core centromere and pericentromeric regions. (2009)
  • Author(s): Sakuno, T.
  • Journal Title: Chromosome Res. 17 Pages: 239-249
  • Peer Reviewed
• [Journal Article] Human Sgol Down-regulation Leads to Chromosomal Instability in Colorectal Cancer. (2009)
  • Author(s): Iwaizumi, I
  • Journal Title: Gut. 58 Pages: 249-260
  • Peer Reviewed
• [Journal Article] Heterochromatin links to centromeric protection by recruiting shugoshin (2008)
  • Author(s): Yamagishi, Y., Sakuno T., Shimura, M., Watanabe, Y.
  • Journal Title: Nature 455 Pages: 251-255
• [Journal Article] Histone deacetylase 3 is required for centromeric H3K4 deacetylation and sister chromatid cohesion. (2008)
  • Author(s): Eot-Houllier, G., Fulcrand, G., Watanabe, Y., Magnaghi-Jaulin, L., Jaulin, C.
  • Journal Title: Genes Dev. 22 Pages: 2639-2644
• [Journal Article] Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins. (2008)
  • Author(s): Omran, H., Kobayashi, D., Olbrich, H., Tsukahara, T., Loges, N. T., Hagiwara, H., Zhang, Q., Leblond, G., O'Toole, E., Hara, C., Mizuno, H., Kawano, H., Fliegauf, M., Yagi, T., Koshida, S., Miyawaki, A., Zentgraf, H., Seithe, H., Reinhardt, R., Watanabe Y., Kamiya, R., Mitchel, D. R., Takeda, H.
  • Journal Title: Nature 456 Pages: 611-616
• [Journal Article] Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells. (2008)
  • Author(s): Lee, J., Kitajima, T.S., Tanno, Y., Yoshida, K., Morita, T., Miyano, T, Miyake, M., Watanabe, Y.
  • Journal Title: Nature Cell Biol. 10 Pages: 42-52
• [Journal Article] Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins (2008)
  • Author(s): Omran, H.
  • Journal Title: Nature 456 Pages: 611-616
  • Peer Reviewed
• [Journal Article] Histone deacetylase 3 is required for centromeric H3K4 deacetylation and sister chromatid cohesion. (2008)
  • Author(s): Eot-Houllier, G.
  • Journal Title: Genes Dev. 22 Pages: 2639-2644
  • Peer Reviewed
• [Journal Article] Chromatid cohesion : acethylation joins the sisters. (2008)
  • Author(s): Tanaka, K.
  • Journal Title: Curr.Biol. 18
  • Peer Reviewed
• [Journal Article] Heterochromatin links to centromeric protection by recruiting shugoshin. (2008)
  • Author(s): Yamagishi, Y.
  • Journal Title: Nature 455 Pages: 251-255
  • Peer Reviewed
• [Journal Article] Unified mode of centromeric protection by shugoshin in mammali an oocytes and somatic cells. (2008)
  • Author(s): Lee, J., Kitajima, T.S., Tanno, Y., Yoshida, K., Morita, T., Miyano, T, Miyake, M., and Watanabe, Y.
  • Journal Title: Nature Cell Biol. 10 Pages: 42-52
  • Peer Reviewed
• [Journal Article] Aurora controls sister kinetochore mono-orientation and homolog bi-orientation in meiosis-I. (2007)
  • Author(s): Hauf, S., Biswas A., Langegger, M., Kawashima, S.A., Tsukahara, T., Watanabe, Y.
  • Journal Title: EMBO J. 26 Pages: 4475-4486
• [Journal Article] Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres. (2007)
  • Author(s): Kawashima, S.A., Tsukahara, T., Langegger, M., Hauf, S., Kitajima, T.S., Watanabe, Y.
  • Journal Title: Genes Dev. 21 Pages: 420-435
• [Journal Article] Aurora controls sister kinetochore mono-orientation and homolog bi-orientation in meiosis-I. (2007)
  • Author(s): Hauf, S., Biswas A., Langegger, M., Kawashima, S.A., Tsukahara, T., and Watanabe Y.
  • Journal Title: EMBO J. 26 Pages: 4475-4486
  • Peer Reviewed
• [Journal Article] Sister Chromatid Cohesion and Centromere Organization in Meiosis (2007)
  • Author(s): Tanaka, K. and Watanabe, Y.
  • Journal Title: Genome Dynamics and Stability 2 Pages: 57-75
  • Peer Reviewed
• [Journal Article] Chromosome cohesion in mitosis and meiosis. (2007)
  • Author(s): Ishiguro, K., Watanabe, Y.
  • Journal Title: J. Cell Sci. 120 Pages: 367-369
• [Journal Article] Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres. (2007)
  • Author(s): Kawashima S.A., Tsukahara, T., Langegger, M., Hauf, S., Kitajima, T.S., Watanabe.Y.
  • Journal Title: Genes Dev. 21 Pages: 420-435
• [Journal Article] Shugoshin collaborates with protein phosphatase 2A to protect cohesin. (2006)
  • Author(s): Kitajima, T.S., Sakuno, T., Ishiguro, K., Iemura, S., Natsume, T., Kawashima, S.A., Watanabe, Y.
  • Journal Title: Nature 441 Pages: 46-52
• [Journal Article] A one-sided view of kinetochore attachment in meiosis. (2006)
  • Author(s): Watanabe, Y.
  • Journal Title: Cell 126 Pages: 8-10
• [Journal Article] Selective elimination of messenger RNA prevents an incidence of untimely meiosis. (2006)
  • Author(s): Harigaya, Y., Tanaka, H., Yamanaka, S., Tanaka, K., Watanabe, Y., Tsutsumi, C., Chikashige, Y., Hiraoka, Y., Yamashita, A., Yamamoto, M.
  • Journal Title: Nature 442 Pages: 45-50
• [Journal Article] A one-sided view of kinetochore attachment in meiosis. (2006)
  • Author(s): Watanabe. Y.
  • Journal Title: Cell 126 Pages: 8-10
• [Journal Article] Shugoshin collaborates with protein phosphatase 2A to protect cohesin. (2006)
  • Author(s): Kitajima, T.S., SaKuno, T., Ishiguro, K., Iemura, S., Natsume T., Kawashima, S.A., Watanabe.Y.
  • Journal Title: Nature 441 Pages: 46-52
• [Journal Article] Selective elimination of messenger RNA prevents an incidence of untimely meiosis. (2006)
  • Author(s): Harigaya, Y., Tanaka, H., Yamanaka, S., Tanaka, K., Watanabe, Y., Tsutsumi, C, Chikashige, Y., Hiraoka, Y., Yamashita, A., Yamamoto, M.
  • Journal Title: Nature 442 Pages: 45-50
• [Journal Article] Shugoshin: guardian spirit at the centromere. (2005)
  • Author(s): Watanabe, Y.
  • Journal Title: Curr. Opin. Cell Biol. 17 Pages: 590-595
• [Journal Article] The kinetochore protein Moa1 enables cohesion-mediated monopolar attachment at meiosis I. (2005)
  • Author(s): Yokobayashi, S., Watanabe, Y.
  • Journal Title: Cell 123 Pages: 803-817
• [Journal Article] Human Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting shugoshin localization. (2005)
  • Author(s): Kitajima, T.S., Hauf, S., Ohsugi, M., Yamamoto, T., Watanabe, Y.
  • Journal Title: Curr. Biol. 15 Pages: 353-359
• [Journal Article] Sister chromatid cohesion along arms and at centromeres. (2005)
  • Author(s): Watanabe, Y.
  • Journal Title: Trends Genet. 27 Pages: 405-412
• [Journal Article] The importance of being Smc5/6. (2005)
  • Author(s): Watanabe, Y.
  • Journal Title: Nature Cell Biol. 7 Pages: 329-331
• [Journal Article] The importance of being Smc5/6. (2005)
  • Author(s): Watanabe, Y.
  • Journal Title: Nature Cell Biol. 5 Pages: 239-331
• [Journal Article] Sister chromatid cohesion along arms and at centromeres. (2005)
  • Author(s): Watanabe, Y.
  • Journal Title: Trends Genet. 5 Pages: 405-412
• [Journal Article] Shugoshin : guardian spirit at centromere. (2005)
  • Author(s): Watanabe, Y.
  • Journal Title: Curr.Opin.Cell Biol. 17 Pages: 590-595
• [Presentation] Kinetochore geometry defined by cohesion within the centromere (2008)
  • Author(s): Watanabe, Y.
  • Organizer: Chromosome Segregation Centromeres and Kinetochores
  • Place of Presentation: Arcachon, France
  • Year and Date: 2008-10-01
• [Presentation] The Primary requirement of SWI6/HP1 for meiotic chromosome segregation (2008)
  • Author(s): Watanabe, Y.
  • Organizer: Asia-Pacific Regional S.pombe Meeting
  • Place of Presentation: Singapore, Singapore
  • Year and Date: 2008-07-27
• [Presentation] Two distinct categories of centromeric cohesion determine the orientation of kinetochores (2008)
  • Author(s): Watanabe, Y.
  • Organizer: FASEB Summer Research Conference
  • Place of Presentation: Arizona, U.S.A
  • Year and Date: 2008-06-24
• [Presentation] Direct link between heterochromatin and centromeric protector shugoshin (2008)
  • Author(s): Watanabe, Y.
  • Organizer: Gordon Research Conference on Meiosis
  • Place of Presentation: New Hampshire, U.S.A
  • Year and Date: 2008-06-10
• [Presentation] Distribution of cohesion within the centromere regulates kinetochore geometry (2008)
  • Author(s): Watanabe, Y.
  • Organizer: LRI Symposium on Chromosome Biology
  • Place of Presentation: London, U.K
  • Year and Date: 2008-05-30
• [Presentation] Kinetochore geometry defined by cohesion within the centromere (2008)
  • Author(s): Sakuno T., Tada, K., Watanabe, Y.
  • Organizer: Chromosome Segregation Centromeres and Kinetochores
  • Place of Presentation: in Arcachon, France.
• [Presentation] The Primary requirement of SWI6/HP1 for meiotic chromosome segregation (2008)
  • Author(s): Watanabe, Y.
  • Organizer: Asia-Pacific Regional S. pombe Meeting
  • Place of Presentation: in Singapore
• [Presentation] Two distinct categories of centromeric cohesion determine the orientation of kinetochores (2008)
  • Author(s): Watanabe, Y.
  • Organizer: FASEB Summer Research Conference
  • Place of Presentation: in Arizona, U.S. A
• [Presentation] Direct link between heterochromatin and centromeric protector shugoshin (2008)
  • Author(s): Watanabe, Y.
  • Organizer: Gordon Research Conference on Meiosis
  • Place of Presentation: in New Hampshire, U.S.A.
• [Presentation] Distribution of cohesion within the centromere regulates kinetochore geometry (2008)
  • Author(s): Watanabe, Y.
  • Organizer: LRI Symposium on Chromosome Biology
  • Place of Presentation: in London, U. K
• [Presentation] Two distinct categories of centromeric cohesion determine the orientation of kinetochores (2007)
  • Author(s): Watanabe, Y.
  • Organizer: Chromosome and genome stability & instability
  • Place of Presentation: in Osaka, Japan.
• [Presentation] Unified mode of centromeric protection by shugoshin in germ and somatic cells (2007)
  • Author(s): Watanabe, Y.
  • Organizer: 8th European Meiosis Meeting in Japan
  • Place of Presentation: in Shonan, Japan.
• [Presentation] Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells (2007)
  • Author(s): Watanabe, Y.
  • Organizer: 32th FEBS Congress Molecular Machines
  • Place of Presentation: in Vienna, Austria.
• [Presentation] Pericentromeric cohesion is sufficient for the suppression of the chromosome segregation defect in swi6Δ (2007)
  • Author(s): Sakuno, T., Watanabe, Y.
  • Organizer: Forth international Fission Yeast Meeting
  • Place of Presentation: in Copenhagen, Denmark.
• [Presentation] Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres (2007)
  • Author(s): Kawashima, S.A., Tsukahara, T., Langegger, M., Hauf, S., Kitajima, T.S., Watanabe, Y.
  • Organizer: Forth international Fission Yeast Meeting
  • Place of Presentation: in Copenhagen, Denmark.
• [Presentation] Centromeric cohesion is crucial for chromosome segregation (2007)
  • Author(s): Watanabe, Y.
  • Organizer: Forth international Fission Yeast Meeting
  • Place of Presentation: in Copenhagen, Denmark.
• [Presentation] Centromeric cohesion is crucial for chromosome segregation (2007)
  • Author(s): Watanabe, Y.
  • Organizer: Joint Meeting of JSDB & JSCB
  • Place of Presentation: in Fukuoka, Japan.
• [Presentation] Fission yeast Shugoshins play crucial roles in chromosome segregation (2007)
  • Author(s): Watanabe, Y.
  • Organizer: The 7th MBSJ Spring Symposium
  • Place of Presentation: in Hyogo, Japan.
• [Presentation] Fission yeast Shugoshins play crucial roles in chromosome segregation (2007)
  • Author(s): Watanabe, Y.
  • Organizer: The 7th MBSJ Spring Symposium
  • Place of Presentation: Hyogo
• [Presentation] Centromeric cohesion is crucial for chromosome segregation (2007)
  • Author(s): Watanabe, Y.
  • Organizer: Joint Meeting of JSDB & JSCB
  • Place of Presentation: Fukuoka
• [Presentation] Centromeric cohesion is crucial for chromosome segregation (2007)
  • Author(s): Watanabe, Y.
  • Organizer: Forth international Fission Yeast Meeting
  • Place of Presentation: Copenhagen
• [Presentation] Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres (2007)
  • Author(s): Kawashima, S.A., Tsukahara, T., Langegger, M., Hauf, S., Kitajima, T.S. and Watanabe, Y.
  • Organizer: Forth international Fission Yeast Meeting
  • Place of Presentation: Copenhagen
• [Presentation] Pericentromeric cohesion is sufficient for the suppression of the chromosome segregation defect in swi6D (2007)
  • Author(s): Sakuno, T. and Watanabe, Y.
  • Organizer: Forth international Fission Yeast Meeting
  • Place of Presentation: Copenhagen
• [Presentation] Unified mode of centromeric protection by shugoshin in mammalianoocytes and somatic cells (2007)
  • Author(s): Watanabe, Y.
  • Organizer: 32th FEBS Congress Molecular Machines
  • Place of Presentation: Vienna
• [Presentation] Shugoshin collaborates with PP2A to protect cohesin (2006)
  • Author(s): Watanabe, Y.
  • Organizer: FASEB Summer Research Conference
  • Place of Presentation: in California, U.S.A.
• [Presentation] Shugoshin collaborates with PP2A to protect cohesin (2006)
  • Author(s): Watanabe, Y.
  • Organizer: IUBMB
  • Place of Presentation: in Kyoto, Japan.
• [Presentation] Shugoshin collaborates with PP2A to protect centromeric Rec8 at meiosis (2006)
  • Author(s): Sakuno, T., Kawashima, S., Kitajima, T.S., Watanabe, Y.
  • Organizer: Gordon Research Conference on Meiosis
  • Place of Presentation: in New Hampshire, U.S.A.
• [Presentation] Shugoshin collaborates with protein phosphatase 2A to protect centromeric cohesion (2005)
  • Author(s): Watanabe, Y.
  • Organizer: Cell Cycle and Development
  • Place of Presentation: Nagoya
• [Presentation] Sister Chromatid Cohesion at the Centromeric Central Core Regulates Kinetochore Orientation (2005)
  • Author(s): Watanabe, Y.
  • Organizer: International Symposium "DNA Replication and Cell Cycle
  • Place of Presentation: Tokyo
• [Presentation] Regulation of kinetochore orientation in meiosis (2005)
  • Author(s): Watanabe, Y.
  • Organizer: 7th European Meiosis Meeting
  • Place of Presentation: in Madrid, Spain
• [Presentation] Regulation of kinetochore orientation in meiosis (2005)
  • Author(s): Watanabe, Y.
  • Organizer: XXIInd International Conference on Yeast Genetics and Molecular Biology
  • Place of Presentation: in Slovak Republic
• [Book] Sister Chromatid Cohesion and Centromere Organization in Meiosis Genome Dynamics and Stability - Recombination and Meiosis: Crossing-over and Disjunction (Richard Egel & Dirk Lankenau, Eds. ) (2007)
  • Author(s): Tanaka, K., Watanabe, Y.
  • Publisher: Springer Verlag
• [Remarks] 生殖細胞の細胞分裂カギ握るたんぱく質発見(日経産業新聞2005. 12. 2)
• [Remarks] 減数分裂の必須たんぱく質ダウン症・不妊症解明へ(日刊工業2005. 11. 29)
• [Remarks] 生殖細胞分裂で重要たんぱく質東大などのチーム発見(日本経済新聞2005. 12. 2)
• [Remarks] 生殖細胞分裂カギ握るたん白質発見不妊症など原因究明に道(化学工業新聞2005. 12. 2)
• [Remarks] 「減数分裂」に必要なたんぱく質東大教授ら発見不妊症など原因究明に光(読売新聞2005. 12. 7)
• [Remarks] 均等分裂、仕組み解明東大、ダウン症研究に道(日経産業新聞2009. 4. 14)
• [Remarks] 染色体を動かす"動原体"方向づけのメカニズム-渡邊・東大教授ら解明-(科学新聞2009. 4. 24)
• [Remarks] 染色体の分裂一部解明(朝日新聞2009. 5. 1)
• [Remarks]
  • URL: http://www.iam.u-tokyo.ac.jp/watanabe-lab/
• [Patent(Industrial Property Rights)] タンパク質相互作用、抗がん剤、並びにそれらのスクリーニング方法 (2006)
  • Inventor(s): 夏目徹、渡邊嘉典、北島智也
  • Industrial Property Rights Holder: 独立行政法人産業技術総合研究所
  • Industrial Property Number: 2006-002924
  • Filing Date: 2006-01-10
• [Patent(Industrial Property Rights)] 特許出願 (2006)
  • Inventor(s): 夏目 徹, 渡邊 嘉典, 北島 智也
  • Industrial Property Rights Holder: 独立行政法人産業技術総合研究所
  • Filing Date: 2006-01-10