Interaction of cancer cells with basement membrane proteins

• Project/Area Number: 17014077
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Yokohama City University
• Principal Investigator: MIYAZAKI Kaoru Yokohama City University, 大学院・生命ナノシステム科学研究科, 教授 (70112068)
• Co-Investigator(Kenkyū-buntansha): HIGASHI Shouichi 横浜市立大学, 大学院・生命ナノシステム科学研究科, 准教授 (10275076)
• Co-Investigator(Renkei-kenkyūsha): OGAWA Takashi 横浜市立大学, 大学院・生命ナノシステム科学研究科, 特任助教 (80405018)
• Project Period (FY): 2005 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥46,800,000 (Direct Cost: ¥46,800,000); Fiscal Year 2009: ¥9,100,000 (Direct Cost: ¥9,100,000); Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥9,100,000); Fiscal Year 2007: ¥10,100,000 (Direct Cost: ¥10,100,000); Fiscal Year 2006: ¥10,100,000 (Direct Cost: ¥10,100,000); Fiscal Year 2005: ¥8,400,000 (Direct Cost: ¥8,400,000)
• Keywords: がん転移 / 基底膜 / 浸潤 / ラミニン / MMP / がん浸潤 / 転移 / 細胞接着分子 / 上皮-間葉変換(EMT) / 細胞外マトリックス / がん / 浸潤・転移 / 腫瘍増殖 / プロテアーゼ / 阻害剤 / マトリライシン / 細胞移動 / ラミニン5 / マトリプターゼ / 細胞接着

Research Abstract

Tumor microenvironments play important roles in tumor progression. The present study demonstrated that the cell adhesion protein laminin-332 (Lm332 ; α3β3γ2) in the basement membrane was converted to a soluble factor capable of strongly promoting tumor cell migration by a proteolytic cleavage of its γ2 chain (Lmγ2). We previously showed that Lmγ2 is solely overexpressed when carcinoma cells invade into stroma. Here, we found that the Lmγ2 expression was induced by TGF-β or some other factors and promoted invasive growth of tumor cells in vivo. Furthermore, we found a novel vascular laminin, laminin-3B11, that was down-regulated in tumor vasculature. The matrix metalloproteinase matrilysin (MMP7) is frequently overexpressed in many types of human cancer and involved in tumor progression and metastasis. Our results suggested that MMP7 might enhance tumor metastasis by binding to cholesterol sulfate of cell membrane and then cleaving cell surface proteins and Lm332. Lmγ2 and MMP7 seem to be good targets for anti-cancer treatments.

Research Products

• [Journal Article] Down-regulation of a newly identified laminin, laminin-3B11, in vascular basement membranes of invasive human breast cancers. (2011)
  • Author(s): Mori, T., Kariya, Y., Komiya, T., Higashi, S., Miyagi, Y., Sekiguchi, K., Miyazaki, K.
  • Journal Title: Cancer Sci. 102 Pages: 1095-1100
  • NAID: 10029293987
  • Peer Reviewed
• [Journal Article] Downregulation of a newly identified laminin, laminin-3B11, in vascular basement membranes of invasive human breast cancers. (2011)
  • Author(s): Mori, T., Miyazaki, K., et al.
  • Journal Title: Cancer Sci. Volume: 102 Pages: 1095-1100
  • NAID: 10029293987
  • Peer Reviewed
• [Journal Article] Laminin-3B11, a novel vascular type laminin capable of inducing prominent lamellipodial protrusions in microvascular endothelial cells. (2010)
  • Author(s): Mori, T., Ono, K., Kariya, Y., Ogawa, T., Higashi, S., Miyazaki, K.
  • Journal Title: J.Biol.Chem. 285 Pages: 35068-35078
  • Peer Reviewed
• [Journal Article] Production of active MMP7 in E.Coli and its application for metalloproteinase inhibitors screening. (2010)
  • Author(s): Katsuno, H., Shirakawa, R., Miyazaki, K., Ozeki, Y., Yasumitsu, H.
  • Journal Title: Protein Peptide Lett. 17 Pages: 568-572
  • Peer Reviewed
• [Journal Article] Cholesterol sulfate alters substrate preference of matrix metalloproteinase-7 and promotes degradations of pericellular laminin-332 and fibronectin. (2010)
  • Author(s): Yamamoto, K., Miyazaki, K., Higashi, S.
  • Journal Title: J.Biol.Chem. 285 Pages: 28862-28873
  • Peer Reviewed
• [Journal Article] Expression of laminin gamma2 chain monomer enhances invasive growth of human carcinoma cells in vivo. (2010)
  • Author(s): Tsubota, Y., Ogawa, T., Oyanagi, J., Nagashima, Y., Miyazaki, K.
  • Journal Title: Int.J.Cancer 127 Pages: 2031-2041
  • Peer Reviewed
• [Journal Article] Production of active MMP7 in E.Coli and its application for metalloproteinase inhibitors screening. (2010)
  • Author(s): Katsuno, H., Miyazaki, K., et al.
  • Journal Title: Protein Peptide Lett. Volume: 17 Pages: 568-572
  • Peer Reviewed
• [Journal Article] Expression of laminin gamma2 chain monomer enhances invasive growth of human carcinoma cells in vivo. (2010)
  • Author(s): Tsubota, Y., Miyazaki, K., et al.
  • Journal Title: Int.J.Cancer Volume: 127 Pages: 2031-2041
  • Peer Reviewed
• [Journal Article] Cholesterol sulfate alters substrate preference of matrix metalloprotein ase-7 and promotes degradations of pericellular laminin-332 and fibronectin. (2010)
  • Author(s): Yamamoto, K., Miyazaki, K., et al.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 28862-28873
  • Peer Reviewed
• [Journal Article] Laminin-3B11, a novel vascular type laminin capable of inducing prominent lamellipodial protrusions in microvascular endothelial cells. (2010)
  • Author(s): Mori, T., Miyazaki, K., et al.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 35068-35078
  • Peer Reviewed
• [Journal Article] New marker proteins of the horny layer for evaluating the skin condition of patients with atopic dermatitis. (2009)
  • Author(s): Yamane, Y., Moriyama, K., Yasuda, C., Miyata, S., Aihara, M., Ikezawa, Z., Miyazaki, K.
  • Journal Title: Int.Arch.Allergy Immunol. 150 Pages: 89-101
  • Peer Reviewed
• [Journal Article] Matrilysin (MMP-7) cleaves C-type lectin domain family 3 member A (CLEC3A) on tumor cell surface and modulates its cell adhesion activity. (2009)
  • Author(s): Tsunezumi, J., Higashi, S., Miyazaki, K.
  • Journal Title: J.Cell.Biochem. 106 Pages: 693-702
  • Peer Reviewed
• [Journal Article] New horny layer marker proteins for evaluating skin condition in atopic dermatitis. (2009)
  • Author(s): Yamane, Y., Miyazaki, K., et al.
  • Journal Title: Int.Arch.Allergy Immunol. Volume: 150 Pages: 89-101
  • Peer Reviewed
• [Journal Article] Matrilysin (MMP-7) cleaves C-type lectin domain family 3 member A (CLEC3A) on tumor cell surface and modulates its cell adhesion activity. (2009)
  • Author(s): Tsunezumi, J., Miyazaki, K., et al.
  • Journal Title: J.Cell.Biochem. Volume: 106 Pages: 693-702
  • Peer Reviewed
• [Journal Article] New marker proteins of the horny layer for evaluating the skin condition of patients with atopic dermatitis (2009)
  • Author(s): Yamane, Y., Moriyama, K., Yasuda, C., Miyata, S., Aihara, M., Ikezawa, Z., Miyazaki, K
  • Journal Title: Arch. Allergy Immunol 150 Pages: 89-101
  • Peer Reviewed
• [Journal Article] Matrilysin (MMP-7) cleaves C-type lectin domain family 3 member A (CLEC3A) on tumor cell surface and modulates its cell adhesion activity (2009)
  • Author(s): Tsunezumi, J., Higashi, H., Miyazaki, K
  • Journal Title: J. Cell. Biochem 106 Pages: 693-702
  • Peer Reviewed
• [Journal Article] Idenitification of amino acid residues of matrix metalloproteinase-2 essential for its selective inhibition by beta-amyloid precursor protein-derived inhibitor. (2008)
  • Author(s): Higashi, S., Miyazaki, K.
  • Journal Title: J.Biol.Chem. 283 Pages: 10068-10078
  • Peer Reviewed
• [Journal Article] Localization of laminin alpha3B chain in vascular and epithelial basement membranes of normal human tissues and its down-regulation in skin cancers. (2008)
  • Author(s): Kariya Y, Mori T, Yasuda C, Watanabe N, Kaneko Y, Nakashima Y, Ogawa T, Miyazaki K.
  • Journal Title: J.Mol.Histol. 39 Pages: 435-446
  • Peer Reviewed
• [Journal Article] Matrilysin (matrix metallo- protease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces. (2008)
  • Author(s): Tsunezumi J, Yamamoto K, Higashi S, Miyazaki K.
  • Journal Title: FEBS J. 275 Pages: 4810-4823
  • Peer Reviewed
• [Journal Article] Identification of amino acid residues of matrix metalloproteinase-7 essential for binding to cholesterol sulfate. (2008)
  • Author(s): Higashi, S., Oeda, M., Yamamoto, K., Miyazaki, K.
  • Journal Title: J.Biol.Chem. 283 Pages: 35735-35744
  • Peer Reviewed
• [Journal Article] Identification of amino acid residues of matrix metalloproteinase-2 essential for its selective inhibition by beta-amyloid precursor protein-derived inhibitor (2008)
  • Author(s): Higashi, S. and Miyazaki, K
  • Journal Title: J Biol Chem 283 Pages: 10068-10078
  • Peer Reviewed
• [Journal Article] Localization of laminin alpha3B chain in vascular and epithelial basement membranes of normal human tissues and its down-regulation in skin cancers (2008)
  • Author(s): Kariya Y, Mori T, Yasuda C, Watanabe N, Kaneko Y, Nakashima Y, Ogawa T, Miyazaki K
  • Journal Title: J Mol Histol 39 Pages: 435-446
  • Peer Reviewed
• [Journal Article] Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces (2008)
  • Author(s): Tsunezumi J, Yamamoto K, Higashi S, Miyazaki K
  • Journal Title: FEBS J 275 Pages: 4810-4823
  • Peer Reviewed
• [Journal Article] Identification of amino acid residues of matrix metalloproteinase-7 essential for binding to cholesterol sulfate (2008)
  • Author(s): Higashi, S., Oeda, M., Yamamoto, K. and Miyazaki, K
  • Journal Title: J Biol Chem 838 Pages: 35735-35744
  • Peer Reviewed
• [Journal Article] 癌の基底膜浸潤におけるEMT-細胞外マトリックス分子の作用 (2008)
  • Author(s): 小川崇、宮崎香
  • Journal Title: 細胞工学 27 Pages: 348-352
• [Journal Article] タンパク質研究のおもしろさ (2008)
  • Author(s): 宮崎香
  • Journal Title: Kihara Memorial Foundation News Letter 25 Pages: 5-7
• [Journal Article] Identification of amino acid residues of matrix metallopro-teinase-2 essential for its selective inhibition by beta-amyloid precursor protein-derived inhibitor. (2008)
  • Author(s): Higashi, S. Miyazaki, K.
  • Journal Title: J Biol Chem. 283 Pages: 10068-10078
  • Peer Reviewed
• [Journal Article] The β3 chain short arm of laminin-332 (laminin-5) induces matrix assembly and cell adhesion activity of laminin-511 (laminin-10). (2007)
  • Author(s): Nakashima, Y., Kariya, Y., Miyazaki, K.
  • Journal Title: J.Cell.Biochem. 100 Pages: 545-556
  • Peer Reviewed
• [Journal Article] The short arm of laminin.2 chain of laminin-5 (laminin-332) binds syndecan-1 and regulates cellular adhesion and migration by suppressing phosphorylation of integrin.4 chain. (2007)
  • Author(s): Ogawa, T., Tsubota, Y., Hashimoto, J., Miyazaki, K.
  • Journal Title: Mol.Biol.Cell 18 Pages: 1621-1633
  • Peer Reviewed
• [Journal Article] Strong suppression of tumor growth by insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1)/TAF/mac25. (2007)
  • Author(s): Sato, Y., Chen, Z., Miyazaki, K.
  • Journal Title: Cancer Sci. 98 Pages: 1055-1063
  • Peer Reviewed
• [Journal Article] Fatima Pedrosa Domellof, F.P. Laminins in keratoconus, bullous keratopathy, normal and scarred human corneas. Histochem. (2007)
  • Author(s): Bystrom, B., Virtanen, I., Rousselle, P., Miyazaki, K., Linden, C.
  • Journal Title: Cell Biol. 127 Pages: 657-667
  • Peer Reviewed
• [Journal Article] A novel function of syndecan-2, suppression of MMP-2 activation, which causes suppression of metastasis. (2007)
  • Author(s): Munesue S., Yoshitomi, Y., Kusano, Y., Koyama, Y., Nishiyama, A., Nakanishi, H., Miyazaki, K., Ishimaru, T., Miyaura, S., Okayama, M., Oguri, K.
  • Journal Title: J.Biol.Chem. 282 Pages: 28164-28174
  • Peer Reviewed
• [Journal Article] Acidic extracellular pH increases calcium influx-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma. (2007)
  • Author(s): Kato, Y., Ozawa, S., Tsukuda, M., Kubota, E., Miyazaki, K., St-Pierre, Y., Hata, R.
  • Journal Title: FEBS J. 274 Pages: 3171-3183
  • Peer Reviewed
• [Journal Article] The β3 chain short arm of laminin-332(laminin-5)inducesmatrix assembly and cell adhesion activity of laminin-511(laminin-10). (2007)
  • Author(s): Nakashima, Y., Miyazaki, K., et. al.
  • Journal Title: J. Cell. Biochem. 100 Pages: 545-556
  • Peer Reviewed
• [Journal Article] The short arm of laminin gamma2 chain of laminin-5(laminin-332)binds syndecan-1 and regulates cellular adhesion and migration by suppressing phosphorylation of integrin beta4 chain. (2007)
  • Author(s): Ogawa, T., Miyazaki, K., et. al.
  • Journal Title: Mol. Biol. Cell 18 Pages: 1621-1633
  • Peer Reviewed
• [Journal Article] Strong suppression of tumor growth by insulin-like growth factor binding protein-related protein 1(IGFBP-rP1)/TAF/mac25. (2007)
  • Author(s): Sato, Y., Miyazaki, K., et. al.
  • Journal Title: Cancer Sci. 98 Pages: 1055-1063
  • Peer Reviewed
• [Journal Article] Laminins in keratoconus, bullous keratopathy, normal and scarred human corneas. (2007)
  • Author(s): Bystrom, B., Miyazaki, K., et. al.
  • Journal Title: Histochem Cell Biol. 127 Pages: 657-667
• [Journal Article] A novel function of syndecan-2, suppression of MMP-2 activa-tion, which causes suppression of metastasis. (2007)
  • Author(s): Munesue S., Miyazaki, K., et. al.
  • Journal Title: J Biol Chem. 282 Pages: 28164-281674
  • Peer Reviewed
• [Journal Article] The alpha3 chain short arm of laminin-332 (laminin-5) induces matrix assembly and cell adhesion activity of laminin-511 (laminin 10). (2007)
  • Author(s): Nakashima, Y., Miyazaki, K.et al.
  • Journal Title: J. Cell. Biochem. 100 Pages: 545-556
• [Journal Article] N-acetylglucosaminyltransferase III antagonizes the effect of N-acetylglucosaminyltransferase V on alpha 3beta 1 integrin-mediated cell migration. (2007)
  • Author(s): Zhao, Y, Miyazaki, K.et al.
  • Journal Title: J Biol Chem (印刷中)
• [Journal Article] Deletion of core fucosylation on alpha3betal integrin down-regualtes its function. (2007)
  • Author(s): Zhao, Y., Miyazaki, K.et al.
  • Journal Title: J Biol Chem. (印刷中)
• [Journal Article] The short arm of laminin gamma2 chain of laminin-5 (laminin-332) binds syndecan-1 and regulates cellular adhesion and migration by suppressing phosphorylation of integrin beta4 chain. (2007)
  • Author(s): Ogawa, T., Miyazaki, K.et al.
  • Journal Title: Mol. Biol. Cell (印刷中)
• [Journal Article] Strong suppression of tumor growth by insulin-like growth factor binding protein-related protein 1(IGFBP-rPI)/TAF/mac25. (2007)
  • Author(s): Sato, Y., Miyazaki.K.et al.
  • Journal Title: Cancer Sci. (印刷中)
• [Journal Article] Laminins in keratoconus, bullous keratopathy normal and scarred human corneas. (2007)
  • Author(s): S Bystrom B., Miyazaki.K.et al.
  • Journal Title: Histochem Cell Biol. (印刷中)
• [Journal Article] Plasma membrane-associated sialidase (NEU3) differentially regulates integrin-mediated cell proliferation through laminin- and fibronectin-derived signaling. (2006)
  • Author(s): Kato, K., Shiga, K., Yamaguchi, K., Hata, K., Kobayashi, T., Miyazaki, K., Saijo, S., Miyagi, T.
  • Journal Title: Biochem.J. 394 Pages: 647-656
  • Peer Reviewed
• [Journal Article] Laminin-5 : Unique biological activity and role in tumor growth and invasion. (2006)
  • Author(s): Miyazaki, K.
  • Journal Title: Cancer Sci. 97 Pages: 91-98
  • Peer Reviewed
• [Journal Article] Reduced expression of laminin-5 in corneal epithelial cells under high glucose condition. (2006)
  • Author(s): Lu W, Ebihara N, Miyazaki K., Murakami A.
  • Journal Title: Cornea. 25 Pages: 61-67
  • Peer Reviewed
• [Journal Article] Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related rrotein-1 (2006)
  • Author(s): Ahmed, S., Jin, X., Yagi, M., Yasuda, C., Sato, Y., Higashi, S., Lin, C-Y., Dickson, R.B., Miyazaki, K.
  • Journal Title: IGFBP-rP1/angiomodulin/mac25.FEBS J. 273 Pages: 615-627
  • Peer Reviewed
• [Journal Article] Binding of active matrilysin to cell surface cholesterol sulfate is essential for its membrane-associated proteolytic action and induction of homotypic cell adhesion. (2006)
  • Author(s): Yamamoto , K., Higashi, S., Kioi, M., Tsunezumi, J., Honke, K., Miyazaki, K.
  • Journal Title: J.Biol.Chem. 281 Pages: 9170-9180
  • Peer Reviewed
• [Journal Article] Insulin-like growth factor-1 induces migration and expression of laminin-5 in cultured human corneal epithelial cells. (2006)
  • Author(s): Lee, H. K., Lee, J. H., Kim, M., Kariya, Y., Miyazaki, K., Kim, E. K.
  • Journal Title: Invest.Ophthalmol.Vis.Sci. 47 Pages: 873-882
  • Peer Reviewed
• [Journal Article] Regulation of proliferation and chondrogenic differentiation of human mesenchymal stem cells by laminin-5 (laminin-332). (2006)
  • Author(s): Hashimoto, J., Kariya, Y., Miyazaki, K.
  • Journal Title: Stem Cells 24 Pages: 2346-2354
  • Peer Reviewed
• [Journal Article] Matriptase activates stromelysin (MMP-3) and promotes tumor growth and angiogenesis. (2006)
  • Author(s): Jin, X, J., Yagi, M., Akiyama, N., Hirosaki, T., Higashi, S., Lin, C-Y., Dickson, R.B., Kitamura, H., Miyazaki, K.
  • Journal Title: Cancer Sci. 97 Pages: 1327-1334
  • NAID: 10020387781
  • Peer Reviewed
• [Journal Article] N-acetylglucosaminyltransferase III antagonizes the effect of N-acetylglucosaminyltransferase V on alpha 3beta 1 integrin-mediated cell migration.J. (2006)
  • Author(s): Zhao, Y., Nakagawa, T., Itoh, S., Inamori, K., Isaji T, Kariya, Y., Kondo, A., Miyoshi, E., Miyazaki, K., Kawasaki, N., Taniguchi, N., Gu, J.
  • Journal Title: Biol.Chem. 281 Pages: 32122-32130
  • Peer Reviewed
• [Journal Article] Deletion of core fucosylation on alpha3beta1 integrin down-regualtes its function. J. (2006)
  • Author(s): Zhao, Y., Itoh, S., Wang, X., Isaji, T., Miyoshi, E., Kariya Y, Miyazaki, K., Kawasaki, N., Taniguchi, N., Gu J.
  • Journal Title: Biol.Chem. 281 Pages: 38343-38350
  • Peer Reviewed
• [Journal Article] Plasma membrane-associated sialidase (NEU3) differentially regulates integrin-mediated cell proliferation through laminin- and fibronectin-derived signaling. (2006)
  • Author(s): Kato, K., Miyazaki, K.et al.
  • Journal Title: Biochem.J. 394 Pages: 647-656
• [Journal Article] Binding of active matrilysin to cell surface cholesterol sulfate is essential for its membrane-associated proteolytic action and induction of homotypic cell adhesion. (2006)
  • Author(s): Yamamoto, K., Miyazaki, K.et al.
  • Journal Title: J.Biol.Chem. 281 Pages: 9170-9180
• [Journal Article] Regulation of proliferation and chondrogenic differentiation of human mesenchymal stem cells by laminin-5 (laminin-332). (2006)
  • Author(s): Hashimoto, J., Miyazaki, K.et al.
  • Journal Title: Stem Cells 24 Pages: 2346-2354
• [Journal Article] Matriptase activates stromelysin (MMP-3) and promotes tumor growth and angiogenesis. (2006)
  • Author(s): Jin, X, J., Miyazaki, K.et al.
  • Journal Title: Cancer Sci. 97 Pages: 1327-1334
  • NAID: 10020387781
• [Journal Article] Murakami A. Reduced expression of laminin-5 in corneal epithelial cells under high glucose condition. (2006)
  • Author(s): Lu, W., Miyazaki, K
  • Journal Title: Cornea. 25 Pages: 61-67
• [Journal Article] Insulin-like growth factor-1 induces migration and expression of laminin-5 in cultured human corneal epithelial cells. (2006)
  • Author(s): Lee, H.K., Miyazaki, K.
  • Journal Title: Invest.Ophthalmol Vis.Sci. 47 Pages: 873-882
• [Journal Article] Production of soluble matriptase by human cancer cell lines and cell surface activation of its zymogen by trypsin. (2005)
  • Author(s): Jin, X., Hirosaki, T., Lin, C-Y, Dickson, R. B., Higashi, S., Kitamura, H., Miyazaki, K.
  • Journal Title: J.Cell.Biochem. 95 Pages: 632-647
  • Peer Reviewed
• [Journal Article] Regulation of biological activity and matrix assembly of laminin-5 by COOH-terminal, LG4-5 domain of alfa3 chain. (2005)
  • Author(s): Tsubota, Y., Yasuda, C., Kariya, Y., Ogawa, T., Hirosaki, T., Mizushima, H., Miyazaki, K.
  • Journal Title: J.Biol.Chem. 280 Pages: 14370-14377
  • Peer Reviewed
• [Journal Article] Regulation of matrix metalloproteinase-9 expression by extracellular pH in mouse metastatic melanoma cells. (2005)
  • Author(s): Kato, Y., Lambert, C. A., Colige, A. C., Mineur, P., Noel, A., Frankenne, F., Foidart, J-M., Baba, M., Hata, R., Miyazaki, K., Tsukuda, M.
  • Journal Title: J.Biol.Chem. 280 Pages: 10938-10944
  • Peer Reviewed
• [Journal Article] Ly6 family member C4.4A binds laminins 1 and 5, associates with galectin-3 and supports cell migration. (2005)
  • Author(s): Paret, C., Bourouba, M., Beer, A., Miyazaki, K., Schnolzer, M., Fiedler, S., Zoller, M.
  • Journal Title: Int.J.Cancer 115 Pages: 724-733
  • Peer Reviewed
• [Journal Article] Nephrotic syndrome and aberrant expression of laminin isoforms in glomerular basement membranes in an infant with Herlitz junctional epidermolysis bullosa (2005)
  • Author(s): Hata, D., Miyazaki, M., Seto, S., Kadota, E., Muso, E., Takasu, K., Nakano, A., Tamai, K., Uitto, J., Nagata, M., Moriyama, K., Miyazaki, K.
  • Journal Title: Pediatrics 116 Pages: 601-607
  • Peer Reviewed
• [Journal Article] Identification of proteins released by pancreatic cancer cells by multidimensional protein identification technology : a strategy for identification of novel cancer markers. (2005)
  • Author(s): Mauri, P., Scarpa, A., Nascimbeni, A. C., Benazzi, L., Parmagnani, E., Mafficini, A., Peruta, M. D., Bassi, C., Miyazaki, K., Sorio, C.
  • Journal Title: FASEB J. 19 Pages: 1125-1127
  • Peer Reviewed
• [Journal Article] Laminin-5 suppresses chondrogenic differentiation of murine teratocarcinoma cell line ATDC5. (2005)
  • Author(s): Hashimoto, J., Ogawa, T., Tsubota, Y., Miyazaki, K.
  • Journal Title: Exp.Cell.Res. 310 Pages: 256-269
  • Peer Reviewed
• [Journal Article] Regulation of cell adhesion and type VII collagen binding by beta3 chain short arm of laminin-5: effect of its proteolytic cleavage. (2005)
  • Author(s): Nakashima, Y., Kariya, Y., Yasuda, C., Miyazaki, K.
  • Journal Title: J.Biochem. 138 Pages: 539-552
  • Peer Reviewed
• [Journal Article] Uncoordinated production of Laminin-5 chains in airways epithelium of allergic asthmatics. Respir. (2005)
  • Author(s): Amin, K., Janson, C., Seveus, L., Miyazaki, K., Virtanen, I., Venge, P.
  • Journal Title: Res. 6 Pages: 110-110
  • Peer Reviewed
• [Journal Article] P.D.A simplified laminin nomenclature. (2005)
  • Author(s): Aumailley, M., Bruckner-Tuderman, L., Carter, W. G., Deutzmann, R., Edgar, D., Ekblom, P., Engel, J., Engvall, E., Hohenester, E., Jones, J. C., Kleinman, H. K., Marinkovich, M. P., Martin, G. R., Mayer, U., Meneguzzi, G., Miner, J. H., Miyazaki, K., Patarroyo, M., Paulsson, M., Quaranta, V., Sanes, J. R., Sasaki,T., Sekiguchi, K., Sorokin, L. M., Talts, J. F., Tryggvason, K., Uitto, J., Virtanen, I., von der Mark, K., Wewer, U. M., Yamada, Y., Yurchenco
  • Journal Title: Matrix Biol. 24 Pages: 326-332
  • Peer Reviewed
• [Journal Article] Differential expression of laminin isoforms in ovarian epithelial carcinomas suggesting different origin and providing tools for differential diagnosis. (2005)
  • Author(s): Maatta, M., Butzow, R., Luostarinen, J., Petajaniemi, N., Pihlajaniemi, T., Salo, S., Miyazaki, K., Autio-Harmainen, H., Virtanen I.
  • Journal Title: J.Histochem.Cytochem. 53 Pages: 1293-1300
  • Peer Reviewed
• [Journal Article] Production of soluble matriptase by human cancer cell lines and cell surface activation of its zymogen by trypsin. (2005)
  • Author(s): Jin X., Miyazaki, K.et al.
  • Journal Title: J.Cell.Biochem. 95 Pages: 632-647
• [Journal Article] Regulation of biological activity and matrix assembly of laminin-5 by COOH-terminal, LG4-5 domain of alfa3 chain. (2005)
  • Author(s): Tsubota, Y., Miyazaki, K.et al.
  • Journal Title: J.Biol.Chem. 280 Pages: 14370-14377
• [Journal Article] Regulation of matrix metalloproteinase-9 expression by extracellular pH in mouse metastatic melanoma cells. (2005)
  • Author(s): Kato, Y., Miyazaki, K.et al.
  • Journal Title: J.Biol.Chem. 280 Pages: 10938-10944
• [Journal Article] Ly6 family member C4.4A binds laminins 1 and 5, associates with galectin-3 and supports cell migration. (2005)
  • Author(s): Paret, C., Miyazaki, K.et al.
  • Journal Title: Int.J.Cancer 115 Pages: 724-733
• [Journal Article] Nephrotic syndrome and aberrant expression of laminin isoforms in glomerular basement membranes in an infant with Herlitz junctional epidermolysis bullosa, (2005)
  • Author(s): Hata, D., Miyazaki, K.et al.
  • Journal Title: Pediatrics 116 Pages: 601-607
• [Journal Article] Identification of proteins released by pancreatic cancer cells by multidimensional protein identification technology : a strategy for identification of novel cancer markers. (2005)
  • Author(s): Mauri, P., Miyazaki, K.et al.
  • Journal Title: FASEB J. 19 Pages: 1125-1127
• [Journal Article] Laminin-5 suppresses chondrogenic differentiation of murine teratocarcinoma cell line ATDC5. (2005)
  • Author(s): Hashimoto, J., Miyazaki, K.et al.
  • Journal Title: Exp.Cell.Res. 310 Pages: 256-269
• [Journal Article] Regulation of cell adhesion and type VII collagen binding by β3 chain short arm of laminin-5: effect of its proteolytic cleavage. (2005)
  • Author(s): Nakashima, Y., Miyazaki, K.et al.
  • Journal Title: J.Biochem. 138 Pages: 539-552
  • NAID: 10017348087
• [Journal Article] Uncoordinated production of Laminin-5 chains in airways epithelium of allergic asthmatics. (2005)
  • Author(s): Amin, K., Miyazaki, K.et al.
  • Journal Title: Respiratory Research 6 Pages: 110-110
• [Journal Article] A simplified laminin nomenclature. (2005)
  • Author(s): Aumailley, M., Miyazaki, K.et al.
  • Journal Title: Matrix Biol. 24 Pages: 326-332
• [Journal Article] Differential expression of laminin isoforms in ovarian epithelial carcinomas suggesting different origin and providing tools for differential diagnosis. (2005)
  • Author(s): Maatta, M., Miyazaki, K.et al.
  • Journal Title: J Histochem.Cytochem. 53 Pages: 1293-1300
• [Journal Article] Plasma membrane-associated sialidase (NEU3) differentially regulates integrin-mediated cell proliferation through laminin-and fibronectin-derived signaling. (2005)
  • Author(s): Kato, K., Miyazaki, K.et al.
  • Journal Title: Biochem.J. 394 Pages: 647-656
• [Journal Article] Unique biological activity and role in tumor growth and invasion. (2005)
  • Author(s): Miyazaki, K.
  • Journal Title: Cancer Sci. 394 Pages: 91-98
• [Journal Article] Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related rrotein-1 (IGFBP-rP1/angiomodulin/mac25. (2005)
  • Author(s): Ahmed, S., Miyazaki, K.
  • Journal Title: FEBS J. 273 Pages: 615-627
• [Journal Article] Binding of active matrilysin to cell surface cholesterol sulfate is essential for its membrane-associated proteolytic action and induction of homotypic cell adhesion.
  • Author(s): Yamamoto, K., Miyazaki, K.
  • Journal Title: J.Biol.Chem. (in press)
• [Presentation] がんの浸潤・転移を調節する微小環境因子-特に細胞外マトリックス分子とプロテアーゼについて (2010)
  • Author(s): 宮崎香
  • Organizer: 数理医学研究会
  • Place of Presentation: 大阪大学(大阪)
  • Year and Date: 2010-12-17
• [Presentation] In vitroがん浸潤モデルにおけるラミニン332(ラミニン5)の侵潤促進作用 (2010)
  • Author(s): 宮崎香
  • Organizer: 第19回日本がん転移学会学術集会・総会
  • Place of Presentation: 金沢市文化ホール(石川)
• [Presentation] Gene expression signatures at acidic extracellular pH in metastatic melanoma cells. (2010)
  • Author(s): Yasumasa Kato, Kaoru Miyazaki, et al.
  • Organizer: Joint Metastasis Research Society-AACR Conference on Metastasis and the Tumor Microenvironment
  • Place of Presentation: Philadelphia (U.S.A.)
• [Presentation] Molecular design of a highly selective and strong inhibitor against MMP-2. (2010)
  • Author(s): Shouichi Higashi, Kaoru Miyazaki, et al.
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場他(大阪)
• [Presentation] Identification and proper ties of novel vascular laminin, laminin-3B11, that lacks in tumor vasculatures. (2010)
  • Author(s): Mori, T.Miyazaki, K., et al.
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場他(大阪)
• [Presentation] Tumor-suppressive effect of IGFBP-related protein 1 (IGFBP-rP1) and its interaction with tumor stroma. (2010)
  • Author(s): Momoko Furuya, Kaoru Miyazaki, et al.
  • Organizer: 第33回分子生物学会/第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] Interaction of Tumor Invasion Marker Laminin γ2 Chain with Vascular Endothelial Celts. (2010)
  • Author(s): Hiroki Sato, Kaoru Miyazaki, et al.
  • Organizer: 第33回分子生物学会/第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] Purification of human natural laminin-511 using new monoclonal antibody and its application to culture of human mesenchymal stem cells. (2010)
  • Author(s): Toshihide Yanagawa, Kaoru Miyazaki, et al.
  • Organizer: 第33回分子生物学会/第83回日本生化学会合同
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] Cholesterol sulfate alters substrate preference of MMP-7 and promotes degradation of selective pericellular proteins. (2010)
  • Author(s): Shouichi Higashi, Kaoru Miyazaki, et al.
  • Organizer: 第33回分子生物学会/第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] Induction of IGFBP-related protein 1 (IGFBP-rP1) in blood vessels of breast cancer and its mechanism. (2010)
  • Author(s): Marii Ise, Kaoru Miyazaki, et al.
  • Organizer: 第33回分子生物学会/第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] ヒトiPS細胞フィーダーフリー培養系～臨床応用を目指した培養系における組換えヒトLaminin-5の有用性～ (2009)
  • Author(s): 山田宗弘、宮崎香, 他
  • Organizer: 日本分子生物学会春季シンポジウム
  • Place of Presentation: シーガイア ワールドコンベンションセンター・サミット(宮崎)
• [Presentation] Expression of laminina α3B chain in vascular and epithelial basement membranes and its possible functions. (2009)
  • Author(s): Taizo Mori, Kaoru Miyazaki, et al.
  • Organizer: Yokosuka Science Festa 2009 8^<th> Pan-pacitic connective Tissue Societies Symposium.
  • Place of Presentation: 湘南国際村センター(神奈川)
• [Presentation] Insulin-like growth factor binding protein-related protein 1 (1rP1/TAF) synergistically modulates tumor cell adhesion with laminin-332 (laminin-5). (2009)
  • Author(s): Eriko Komiya, Kaoru Miyazaki, Et al.
  • Organizer: Yokosuka Science Festa 2009 8^<th> Pan-pacific connective Tissue Societies Symposium.
  • Place of Presentation: 湘南国際村センター(神奈川)
• [Presentation] がん浸潤マーカー分子ラミニンγ2鎖の機能 (2009)
  • Author(s): 宮崎香、小川崇
  • Organizer: 第18回日本がん転移学会学術集会・総会
  • Place of Presentation: 旭川グランドホテル(北海道)
• [Presentation] がん細胞の基底膜浸潤とEMT (2009)
  • Author(s): 宮崎香
  • Organizer: 第68回日本癌学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] がん細胞におけるラミニンγ2鎖の発現と上皮-間葉変換(EMT)の調節機構 (2009)
  • Author(s): 小柳潤、宮崎香, 他
  • Organizer: 第68回日本癌学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] IGFBP-rP1/TAFとラミニン332のがん細胞に対する強調的な促進作用 (2009)
  • Author(s): 古宮栄利子、宮崎香, 他
  • Organizer: 第68回日本癌学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] がんの基底膜浸潤におけるラミニンγ2鎖の発現と上皮-間葉変換(EMT) (2009)
  • Author(s): 小川崇、宮崎香, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] ラミニン332(ラミニン5)のマトリックスプロテアーゼ誘導能とがんの浸潤性増殖 (2009)
  • Author(s): 佐藤拓輝、宮崎香, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] In vitroがん浸潤モデルを用いたラミニン332(ラミニン5)の浸潤促進作用の解析 (2009)
  • Author(s): 小島奈子、宮崎香, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] マトリプターゼ(MT-SP1)によるCTGF(IGFBP-rP2)の切断と腫瘍増殖促進作用 (2009)
  • Author(s): 寺田翔、宮崎香, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] マトリックスメタプロテアーゼ-2(MMP-1)に対し、高い特異性を持つインヒビタータンパク質の設計 (2009)
  • Author(s): 廣瀬智一、宮崎香, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] ヒトiPS細胞フィーダーフリー培養系～臨床応用を目指した培養系における組換えヒトLaminin-5の有用性～ (2009)
  • Author(s): 山田宗弘、宮崎香, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(兵庫>
• [Presentation] ラミニン5および関連分子を利用した幹細胞培養技術の開発 (2008)
  • Author(s): 宮崎香
  • Organizer: イノベーションジャパン2008
  • Place of Presentation: 東京
  • Year and Date: 2008-09-17
• [Presentation] 細胞外マトリックス分子による腫瘍増殖の調節 (2008)
  • Author(s): 宮崎香
  • Organizer: 文部科学省がん特定領域研究シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-07-04
• [Presentation] ヒトがん細胞におけるラミニン5(ラミニン332)構成鎖の常発現と腫瘍増殖におけるその意義 (2008)
  • Author(s): 小川崇、宮崎香
  • Organizer: 第17回日本がん転移学会学術集会・総会
  • Place of Presentation: 鹿児島
• [Presentation] マトリライシンによるがん細胞表層アネキシンHの切断と組織プラスミノーゲン活性因子の膜への結合 (2008)
  • Author(s): 常住淳、宮崎香
  • Organizer: 第17回日本がん転移学会学術集会・総会
  • Place of Presentation: 鹿児島
• [Presentation] APP由来インヒビターによる選択的活性阻害に重要なMMP-2分子内のアミノ酸残基の同定 (2008)
  • Author(s): 東昌市、宮崎香
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] ラミニンY2鎖の癌の悪性増殖やEMTのおける役割 (2008)
  • Author(s): 小川崇、宮崎香
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 酸性細胞外pHによるマトリックス・メタプロテアーゼ・9発現誘導におけるRhoの役割 (2008)
  • Author(s): 加藤靖正、西村行生、佃守、宮崎香、畑隆一郎
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 癌の悪性形質を支えるMMP-2およびMMP-7の細胞表層での活性発現機序とそれを応用した高特異性阻害剤の開発 (2008)
  • Author(s): 東昌市、山本和博、宮崎香
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 癌の基底膜浸潤におけるラミニンY2鎖の発現と上皮-間葉変換(EMT) (2008)
  • Author(s): 小川崇、清水翔太、宮崎香
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Matriptaseによる腫瘍増殖機構の解析一IGFBPsの切断の意義について (2008)
  • Author(s): 迫田明子、山本和博、寺田翔、秋山渚、東昌市、宮崎香
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 新規ラミニンアイソフォームラミニン3B11(ラミニン6B)の発現と機能解析 (2008)
  • Author(s): 森泰三、小川崇、小野孝太、吉村祐太、苅谷慶喜、宮崎香
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Distinct roles of NF-K13 subunits in matrix metalloproteinase-9 expression induced by acidic extracellular pH (2008)
  • Author(s): Yasumasa Kato, Shigeyuki Oza, Eiro Kubota, Mamoru Tsukuda, Kaoru Miyazaki, Yves St-Pierre, Ryu-Icbiro Hata
  • Organizer: Metastasis Research Society-Amer. Asociation for Cancer Res. Joint Conference on Metastasis
  • Place of Presentation: Vancouver
• [Presentation] Possible Biomarkers that Identify Degree of Skin Senescence (2008)
  • Author(s): Chic Yasuda, Satoshi Miyata, Tatsuya Kon, Nakaba Kayanuma, Takayo Ueda, Kohsuke Hayamizu, Hironori Tomi and Kaoru Miyazaki
  • Organizer: 25^<th> IFSCC Congress
  • Place of Presentation: Barcelona
• [Book] 上皮-間葉変換と癌の悪性増殖-細胞外マトリックス分子の役割。「実験医学」4月号Vol.21,No.15 (2006)
  • Author(s): 宮崎香
  • Publisher: 羊土社
• [Book] 細胞外マトリックス分子と細胞接着。「タンパク質科学-構造・物性・機能」 (2005)
  • Author(s): 小川崇, 宮崎香(分担)
  • Publisher: 化学同人
• [Book] タンパク質研究なるほど(戸田年総, 平野久, 中村和行編) (2005)
  • Author(s): 宮崎香(分担)
  • Total Pages: 6
  • Publisher: 羊土社
• [Book] 細胞外マトリックス分子と癌の悪性増殖。「肝転移のすべて」(門田守人, 松浦成昭/編) (2005)
  • Author(s): 宮崎香(分担)
  • Publisher: 永井書店
• [Remarks] ホームページ
  • URL: http://miyazaki.sci.yokohama-cu.ac.jp/
• [Remarks]
  • URL: http://miyazaki.sci.yokohama-cu.ac.jp/
• [Remarks]
  • URL: http://miyazaki.sci.yokohama-cu.ac.jp/
• [Patent(Industrial Property Rights)] ラミニン6B(ラミニン3B11)蛋白質 (2007)
  • Inventor(s): 宮崎香
  • Industrial Property Rights Holder: 公立大学法人横浜市立大学
  • Filing Date: 2007-02-08
• [Patent(Industrial Property Rights)] ラミニン6B(ラミニン3B11)蛋白質 (2007)
  • Inventor(s): 宮崎 香
  • Industrial Property Rights Holder: 横浜市立大学
  • Filing Date: 2007-02-08
• [Patent(Industrial Property Rights)] ラミニン5を利用した間葉系幹細胞の培養技術 (2005)
  • Inventor(s): 宮崎香, 橋本絢子, 苅谷慶喜
  • Industrial Property Rights Holder: 公立大学法人横浜市立大学,財団法人木原記念横浜生命科学振興財団
  • Filing Date: 2005-08-16