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hnRNP A1/D、Musashi及びアプタマーによる遺伝情報制御の分子基盤

Research Project

Project/Area Number 17026011
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionYokohama City University

Principal Investigator

片平 正人  横浜市立大学, 国際総合科学研究科, 教授 (70211844)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2005: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordshnRNP / Musashi / RNAアプタマー / NMR / 構造 / テロメア / テロメラーゼ / RNAi / 生体分子 / 生物物理 / 蛋白質 / 脳・神経 / エイズ
Research Abstract

Musashiタンパク質のタンデムな2つのRNA結合ドメインと標的RNAの複合体に関して、構造解析を行った。まずRNAとの結合状態にあるMusashiの2つのドメインの構造を、NOE等に基づいて決定できた。次に2つのドメインの相対配向を、残余双極子結合に基づいてイ決定した。さらに長さを変えた様々なRNAを利用する事でMusashiとRNAの間の分子間NOEの同定に成功し、それに基づいた複合体の構造決定を進行させた。常磁性緩和効果による惣ングレンジの構造情報の取得も試みた。
テロメア配列のDNA/RNA結合タンパク質hnRNPA1のタンデムな2つの核酸結合ドメインが、テロメアDNA及びテロメレースRNAとどのような様式で相互作用するのかを明らかにした。これにより同タンパク質によるテロメレースのテロメアDNAへのリクルート機構に関する構造学的な基盤が得られた。またRNAi法を用いてhnRNPA1タンパク質及びhnRNPDタンパク質をノックダウンした細胞におけるテロメア長を測定する事で、両タンパク質の機能に直接迫った。
ヒトのテロメアDNAが生理的なイオン条件下(カリウムイオンに富んだイオン条件下)において形成する特異な4重鎖構造の決定に成功した。これまで考えられていたものとは異なる新規構造が見出され、分子内の構造体でありながら、3本の鎖が平行に配置され、残り1本のみが反平行に配置されていた。

Report

(2 results)
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2007 2006 2005

All Journal Article (16 results) Book (2 results)

  • [Journal Article] Structure of human telomeric DNA under physiological ionic conditions stabilized by proper incorporation of 8-bromoguanosines, as determined by NMR2007

    • Author(s)
      Matsugami, M他
    • Journal Title

      FEBS J (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs2006

    • Author(s)
      Tagashira, H他
    • Journal Title

      Zoological Science 23

      Pages: 9-21

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Structural property of DNA that migrates faster in gelelectrophoresis, as deduced by CD spectroscopy2006

    • Author(s)
      Matsugami, A他
    • Journal Title

      Nucleosides, Nucleotides and Nucleic Acids 25

      Pages: 417-425

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue from the sheath of Leptothrix cholodnii2006

    • Author(s)
      Makita, H他
    • Journal Title

      Biosci. Biotech. & Biochem 70

      Pages: 1265-1268

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Molecular mechanism for maintenance of G-rich short tandem repeats capable of adopting G4 DNA structures2006

    • Author(s)
      Nakagama, H他
    • Journal Title

      Mutat. Res 598

      Pages: 120-131

    • Related Report
      2006 Annual Research Report
  • [Journal Article] NMRによるタンパク質構造解析の展望2006

    • Author(s)
      宮ノ入洋平他
    • Journal Title

      酵素工学ニュース 55

      Pages: 29-34

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Structural analysis of DNA containing (6-4) photoproduct in complex with distamycin A by NMR2006

    • Author(s)
      Tsuchibayashi, H他
    • Journal Title

      Nucleic Acids Symp. Ser. 50

      Pages: 237-238

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The new models of the human telomere DNA in K^+ solution revealed by NMR analysis assisted by the incorporation of 8-bromoguanines2006

    • Author(s)
      Matsugami, A他
    • Journal Title

      Nucleic Acids Symp. Ser 50

      Pages: 42-43

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Structural property of DNA that migrates faster in gel electrophoresis, as deduced by CD spectroscopy2006

    • Author(s)
      Matsugami, A., Tani, K., Ouhashi, K., Uesugi, S., Morita, M., Ohyama, T., Katahira M.
    • Journal Title

      Nucleosides, Nucleotides and Nucleic Acids (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification of 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue from the sheath of Leptothrix cholodnii2006

    • Author(s)
      Makita, H., Nakahara, Y., Fukui, H., Miyanoiri, Y., Katahira M., Seki, H., Takeda, M., Koizumi, J.
    • Journal Title

      Biosci.Biotech.& Biochem. (in press)

    • NAID

      10018531700

    • Related Report
      2005 Annual Research Report
  • [Journal Article] NMRによるタンパク質構造解析の展望-Musashiタンパク質によるRNAの認識機構の解析を例に-2006

    • Author(s)
      宮ノ入洋平, 片平正人
    • Journal Title

      酵素工学ニュース (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structure of hnRNP D : single-stranded telomere DNA complex and unfolding of quadruplex by hnRNP D2005

    • Author(s)
      Enokizono, Y., Konisni, Y., Uesugi, S., Ishikawa, F., Katahira, M.
    • Journal Title

      J.Biol.Chem. 280

      Pages: 18862-18870

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Unfolding of higher DNA structures formed by the d(CGG) triplet repeat by UP1 protein2005

    • Author(s)
      Fukuda, H., Katahira, M., Tanaka, E., Enokizono, Y., Tsuchiya, N., Higuchi, K., Nagao, M., Nakagama, H.
    • Journal Title

      Genes Cells 10

      Pages: 953-962

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Human vault-associated non-coding RNAs bind to mitoxantrone, a chemotherapeutic compound2005

    • Author(s)
      Gopinath, S.C.B., Matsugami, A., Katahira, M., Kumar, P.K.R.
    • Journal Title

      Nucleic Acids Res. 33

      Pages: 4874-4881

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs2005

    • Author(s)
      Tagashira, H., Shimotori, T., Sakamoto, N., Katahira, M., Miyanoiri, Y., Yamamoto, T., Mitsunaga-Nakatsubo K., Kusunoki, S., Akasaka, K.
    • Journal Title

      Zoological Science 23

      Pages: 9-21

    • NAID

      110004775619

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structure of RNA aptamer complexed with an RNA-binding peptide of Tat with aid of residue-epecific ^<13>C, ^<15>N labeling2005

    • Author(s)
      Matsugami, A., Tochio H., Niyada, E., Tamura, Y., Kudo, M., Misono, T., Kumar, P., Katahira, M.
    • Journal Title

      Nucleic Acids Res. Suppl. 5

      Pages: 69-70

    • Related Report
      2005 Annual Research Report
  • [Book] 生物物理学ハンドブック(分担)2007

    • Author(s)
      片平正人
    • Publisher
      朝倉書店
    • Related Report
      2006 Annual Research Report
  • [Book] 第5版 実験化学講座8NMR・ESR(分担)2006

    • Author(s)
      片平正人
    • Publisher
      丸善
    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2018-03-28  

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