Ca^<2+> channelplexes : assembly in the membrane and physiological significance
Project/Area Number |
17081011
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | Kyoto University |
Principal Investigator |
MORI Yasuo Kyoto University, 大学院・工学研究科, 教授 (80212265)
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Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Toyoshi 名古屋大学, 大学院・医学系研究科, 教授 (50115929)
WAKAMORI Minoru 東北大学, 大学院・歯学研究科, 教授 (50222401)
HARA Yuji アイオワ大学, 医学部, ポスドク (60362456)
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Project Period (FY) |
2005 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥76,500,000 (Direct Cost: ¥76,500,000)
Fiscal Year 2009: ¥17,100,000 (Direct Cost: ¥17,100,000)
Fiscal Year 2008: ¥17,100,000 (Direct Cost: ¥17,100,000)
Fiscal Year 2007: ¥17,100,000 (Direct Cost: ¥17,100,000)
Fiscal Year 2006: ¥18,000,000 (Direct Cost: ¥18,000,000)
Fiscal Year 2005: ¥7,200,000 (Direct Cost: ¥7,200,000)
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Keywords | Ca^<2+>チャネル / 分子複合体 / 膜分子集積 / シグナル伝達 / バイオセンサー / カルシウムチャネル |
Research Abstract |
Ca^<2+> as a second messenger controls a wide variety of biological responses and contributes to formation of tissues such as bones, thereby playing a central role in regulating "homeostasis" at whole body, tissue, and cell levels. In my research, I have focused on voltage-dependent Ca^<2+> channels (VDCCs) and transient receptor potential (TRP) channels to aim at understanding mechanisms, that underlie formation and assembly at the biomembrane of Ca^<2+> channel protein complexes (Ca^<2+> channelplexes), and their physiological significance. Firstly, I have unveiled several unique activation mechanisms, that regulate cellular signal transduction for TRP Ca^<2+>-permeable caion channels. Secondly, I have demonstrated that channel proteins act not only as Ca^<2+>-permeating apparatus but also as assembly centers for receptors, signal-regulating proteins, and scaffolding proteins. Thirdly, important roles of Ca^<2+> channel protein complexes via formation of feedback regulation pathways in signal transduction have been demonstrated. Finally, I have also shown physiological significance of Ca^<2+> channelplexes at tissue levels. TRPC3 channelplexes in B lymphocyte activation and P/Q type VDCCplexes in presynaptic vesicle fusion are examples of the outcome of this study. Thus, my study clarifies how individual Ca^<2+> channels play roles in unique cellular responses.
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Report
(6 results)
Research Products
(70 results)
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[Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade.2010
Author(s)
Koike C, Obara T, Uriu Y, Numata T, Sanuki R, Miyata K, Koyasu T, Ueno S, Funabiki K, Tani A, Ueda H, Kondo M, Mori Y, Tachibana M, Furukawa T.
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Journal Title
Proc. Natl. Acad. Sci. USA 107
Pages: 332-337
Related Report
Peer Reviewed
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[Journal Article] An in vivo fluorescent sensor reveals intracellular Ins(1, 3, 4, 5)P(4) dynamics in single cells.2010
Author(s)
Sakaguchi R, Tainaka K, Shimada N, Nakano S, Inoue M, Kiyonaka S, Mori Y, Morii T.
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Journal Title
Angew. Chem. Int. Ed. 17
Pages: 7381-7386
Related Report
Peer Reviewed
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[Journal Article] An in vivo fluorescent sensor reveals intracellular Ins(1, 3, 4, 5)P(4) dynamics in single cells.2010
Author(s)
Sakaguchi R, Tainaka K, Shimad a N, Nakano S, Inoue M, Kiyonaka S, Mori Y, Morii T
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Journal Title
Angew. Chem. Int. Ed. 17
Pages: 7381-7386
Related Report
Peer Reviewed
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[Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade2010
Author(s)
Koike C, Obara T, Uriu Y, Numata T, Sanuki R, Miyata K, Koyasu T, Ueno S, Funabiki K, Tani A, Ueda H, Kondo M, Mori Y, Tachibana M, Furukawa T
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Journal Title
Proc. Natl. Acad. Sci. USA 107, 332-337 107
Pages: 332-337
Related Report
Peer Reviewed
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[Journal Article] Ca^<2+> influx and protein scaffolding via TRPC3 sustain PKC and ERK activation in B cells.2010
Author(s)
Numaga T, Nishida M, Kiyonaka S, Kato K, Katano K, Mori E, Kurosaki T, Inoue R, Hikida T, Putney Jr JW, Mori Y
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Dofferemtoa; redpx regi;atopm pf PRAO Cjammels : a mechanism to tune cellular calcium responses2010
Author(s)
Bogeski I, Kummerow C, Al-Ansary D, Koehler R, Schwarz EC, Kozai D, Takahashi N, Peinelt C, Griesemer D, Bozem B, Mori Y, Hoth M, Niemeyer BA
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Journal Title
Science Signaling (In press)
Related Report
Peer Reviewed
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[Journal Article] A pathogenic C-terminus-truncated Polycistin-2 mutant enhances receptor-activated Ca^<2+> entry via association with TRPC3 and TRPC7.2009
Author(s)
Miyagi K, Kiyonaka S, Yamada K, Miki T, Mori E, Kato K, Numata T, Sawaguchi Y, Numaga T, Kimura T, Kanai Y, Kawano M, Wakamori M, Nomura H, Koni I, Yamagishi M, Mori Y.
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Journal Title
J. Biol. Chem. 284
Pages: 34400-34412
Related Report
Peer Reviewed
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[Journal Article] Suppression of hippocampal TRPM7 protein prevents delayed neuronal death in brain ischemia.2009
Author(s)
Sun H-S, Jackson MF, Martin LJ, Jansen K, Teves L, Cui H, Kiyonaka S, Mori Y, Jones M, Forder JP, Golde TE, Orser BA, MacDonald JF, Tymianski M.
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Journal Title
Nature Neurosci. 12
Pages: 1300-1307
Related Report
Peer Reviewed
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[Journal Article] Targetted disruption of the voltage-dependent Ca^<2+> channelα-2/δ-1 subunit.2009
Author(s)
Fuller-Bicer GA, Varadi G, Koch SE, Ishii M, Bodi I, Kadeer N, Muth JN, Mikala G, Petrashevskaya NN, Jordan MA, Zhang SP, Qin N, Flores CM, Isaacsohn I, Varadi M, Mori Y, Jones WK, Schwartz A.
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Journal Title
Am. J. Physiol. Heart Circ. Physiol. 297
Related Report
Peer Reviewed
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[Journal Article] Synergistic activation of vascular TRPC6 channel by receptor and mechanical stimulation via PLC/diacylglycerol and PLA-2/£s-hydroxylase/20-HETE pathways.2009
Author(s)
Inoue R, Jensen LJ, JianZ, Shi J, Hai L, Lurie AI, Henriksen FH, Salmonsson M, Morita H, Kawarabayashi Y, Mori M, Mori Y, Ito Y.
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Journal Title
Circ Res. 104
Pages: 1399-1409
Related Report
Peer Reviewed
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[Journal Article] STIM protein coupling in the activation of Orai channels.2009
Author(s)
Wang Y, Deng X, Zhou Y, Hendron E, Mancarella S, Ritchie MF, Tang XD, Baba Y, Kurosaki T, Mori Y, Soboloff J, Gill DL.
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Journal Title
Proc. Natl. Acad. Sci. USA 106
Pages: 7391-7396
Related Report
Peer Reviewed
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[Journal Article] Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound.2009
Author(s)
Kiyonaka S, Kato K, Nishida M, Mio K, Numaga T, Sawaguchi Y, Yoshida T, Wakamori M, Mori E, Numata T, Ishii M, Takemoto H, Ojida A, Watanabe K, Uemura A, Kurose H, Morii T, Kobayashi T, Sato Y, Sato C, Hamachi I, Mori Y
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Journal Title
Proc. Natl. Acad. Sci. USA 106
Pages: 5400-5405
Related Report
Peer Reviewed
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[Journal Article] Changes in temperature preferences and energy homeostasis in dystroglycan mutants.2009
Author(s)
Takeuchi K, Nakano Y, Kato U, Kaneda M, Aizu M, Awano W, Yonemura S, Kiyonaka S, Mori Y, Yamamoto D, Umeda M.
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Journal Title
Science 323
Pages: 1740-1743
Related Report
Peer Reviewed
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[Journal Article] Changes in temperature preferences and energy homeostasis in dystroglycan mutants.2009
Author(s)
Takeuc K, Nakan Y, Kato U, Kaneda M, Aizu M, Awano W, Yonemura S, Kiyonaka S, MoriY, Yamamoto D, Umeda M
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Journal Title
Science 323
Pages: 1740-1743
Related Report
Peer Reviewed
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[Journal Article] STIM protein coupling in the activation of Orai channels2009
Author(s)
Wang Y, Deng X, Zhou Y, Hendron E, Mancarella S, Ritchie MF, Tang XD, Baba Y, Kurosaki T, Mori Y, Soboloff J, Gill DL
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Journal Title
Proc. Natl. Acad. Sci. USA 106, 7391-7396 106
Pages: 7391-7396
Related Report
Peer Reviewed
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[Journal Article] Synergistic activation of vascular TRPC6 channel by receptor and mechanical stimulation via PLC/diacylglycerol and PLA_2/£s-hydroxylase/20-HETE pathways.2009
Author(s)
Inoue R, Jensen LJ, JianZ, Shi J, Hai L, Lurie AI, Henriksen FH, Salmonsson M, Morita H, Kawarabayashi Y, Mori M, Mori Y, Ito Y.
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Journal Title
Circ Res.104, 1399-1409 104
Pages: 1399-1409
Related Report
Peer Reviewed
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[Journal Article] Targetted disruption of the voltage-dependent Ca^<2+> channel α_2/δ-1 subunit.2009
Author(s)
Fuller-Bicer GA, Varadi G Koch SE, Ishii M, Bodi I, Kadeer N, Muth JN, Mikala G, Petrashevskaya NN, Jordan MA, Zhang SP, Qin N, Flores CM, Isaacsohn I, Varadi M, Mori Y, Jones WK. Schwartz A
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Journal Title
Am. J.Physiol. Heart Circ. Physiol. 297
Related Report
Peer Reviewed
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[Journal Article] Suppression of hippocampal TRPM7 protein prevents delayed neuronal death in brain ischemia2009
Author(s)
Sun H-S, Jackson MF, Martin LJ, Jansen K, Teves L, Cui H, Kiyonaka S, Mori Y, Jones M, Forder JP, Golde TE, Orser BA, MacDonald JF, Tvmianski M.
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Journal Title
Nature Neurosci. 12
Pages: 1300-1307
Related Report
Peer Reviewed
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[Journal Article] A pathogenic C-terminus-truncated Polycistin-2 mutant enhances receptor-a ctivated Ca^<2+> entry via association with TRPC3 and TRPC7.2009
Author(s)
Miyagi K, Kiyonaka S, Yamada K, Miki T, Mori E, Kato K, Numata T, Sawaguchi Y, Numaga T, Kimura T, Kanai Y, Kawano M, Wakamori M, Nomura H, Koni I, Yamagishi M, Mori Y.
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Journal Title
J.Biol. Chem.284, 34400-34412 284
Pages: 34400-34412
Related Report
Peer Reviewed
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[Journal Article] TRPM2-mediated Ca^<2+> influx induces chemokine production in monocytes that aggravates inflammatory neutrophil infiltration2008
Author(s)
Yamamoto S, Shimizu S, Kiyonaka S, Takahashi N, Wajima T, Hara Y, Negoro T, Hiroi T, Kiuchi Y, Okada T, Kaneko S, Lange I, Fleig A, Penner R, Nishi M, Takeshima H, Mori Y.
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Journal Title
Nature Med. 14
Pages: 738-747
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[Journal Article] RIM1 confers sustained activity and neurotransmitter vesicle anchoring to presynaptic Ca^<2+> channels.2007
Author(s)
Kiyonaka S, Wakamori M, Miki T, Uriu Y, Nonaka M, Bito H, Beedle AM, Mori E, Hara Y, De Waard M, Kanagawa M, Itakura M, Takahashi M, Campbell KP, Mori Y
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Journal Title
Nature Neurosci. 10
Pages: 691-701
Related Report
Peer Reviewed
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[Journal Article] RIMI confers sustained activity and neurotransmitter vesicle anchoring to presynaptic Ca2+ channels.2007
Author(s)
Kiyonaka S, Wakamori M, Miki T, Uriu Y, Nonaka M, Bito H, Beedle AM, Mori E, Hara Y, De Waard M, Kanagawa M, Itakura M, Takahashi M, Campbell KP & Mori Y
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Journal Title
Nature Neurosci. 10
Pages: 691-701
Related Report
Peer Reviewed
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[Journal Article] TRPM7 channels in hippocampal neurons detect levels of extracellular divalent cations.2007
Author(s)
Wei W-L, Sun H-S, Olah ME, Sun XiuJun, Czerwinska E, Czerwinski W, Mori Y, Orser BA, Xiong Z-G, Jackson MF, Tymianski M & MacDonald JF
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Journal Title
Proc. Natl. Acad. Sci. USA 104
Pages: 16323-16328
Related Report
Peer Reviewed
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[Journal Article] Coupling of STIM1 to store-operated Ca^<2+> entry through its constitutive and inducible movement in the endoplasmic reticulum.2006
Author(s)
Baba Y, Hayashi K, Fujii Y, Mizushima A, Watarai H, Wakamori M, Numaga T, Mori Y, Iino M, Hikida M, Kurosaki T
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Journal Title
Proc. Natl. Acad. Sci. USA 103
Pages: 16704-16709
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[Journal Article] The role of canonical transient receptor potential 7 (TRPC7) in B-cell receptor-activated channels.2005
Author(s)
Lievremont J-P, Numaga T, Vazquez G, Lemonnier L, Hara Y, Mori E, Trebak M, Moss SE, Bird GS, Mori Y, Putney Jr JW
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Journal Title
J. Biol. Chem. 280
Pages: 35346-35351
Related Report
Peer Reviewed
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[Journal Article] Comprehensive analysis of the ascidian genome reveals novel insights into the molecular evolution of ion channel genes2005
Author(s)
Okamura Y, Murata Y, Nakajo K, Iwasaki I, Ohtsuka Y, Tanaka-Kunishima M, Takahashi N, Hara Y, Yoshida T, Nishida M, Okado H, Watari H, Meinertzhagen IA, Nishino A, Satoh N, Takahashi K, Satou Y, Okada Y, Mori Y
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Journal Title
Physiological Genomics 222
Pages: 269-282
Related Report
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[Journal Article] The role of canonical transient receptor potential 7(TRPC7) in B-cell receptor-activated channels.2005
Author(s)
Lievremont J-P, Numaga T, Vazquez G, Lemonnier L, Hara Y, Mori E, Trebak M, Trebak M, Moss SE, Bird GS, Mori Y, Putney Jr JW
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Journal Title
J.Biol.Chem. 280
Pages: 35346-35351
Related Report
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[Journal Article] Differential redox regulation of ORAI channels : a mechanism to tune cellular calcium responses.
Author(s)
Bogeski I, Kummerow C, Al-Ansary D, Koehler R, Schwarz EC, Kozai D, Takahashi N, Peinelt C, Griesemer D, Bozem B, Mori Y, Hoth M, Niemeyer BA
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Journal Title
Science Signaling (in press)
Related Report
Peer Reviewed
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[Journal Article] Ca^<2+> influx and protein scaffolding via TRPC3 sustain PKCβ and ERK activation in B cells.
Author(s)
Numaga T, Nishida M, Kiyonaka S, Kato K, Katano K, Mori E, Kurosaki T, Inoue R, Hikida T, Putney Jr JW, Mori Y
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Related Report
Peer Reviewed
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