Structural analyses of the interaction between extracellular ligands and their receptors

• Project/Area Number: 17082004
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: TAKAGI Junichi Osaka University, 蛋白質研究所, 教授 (90212000)
• Co-Investigator(Kenkyū-buntansha): IWASAKI Kenji 大阪大学, 蛋白質研究所, 准教授 (20342751) ; 禾 晃和 大阪大学, 蛋白質研究所, 助教 (40379102)
• Co-Investigator(Renkei-kenkyūsha): NOGI Terukazu 大阪大学, 蛋白質研究所, 助教 (40379102) ; YASUI Norihisa 大阪大学, 蛋白質研究所, 助教 (90467514)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥148,400,000 (Direct Cost: ¥148,400,000); Fiscal Year 2009: ¥25,200,000 (Direct Cost: ¥25,200,000); Fiscal Year 2008: ¥26,100,000 (Direct Cost: ¥26,100,000); Fiscal Year 2007: ¥26,300,000 (Direct Cost: ¥26,300,000); Fiscal Year 2006: ¥44,700,000 (Direct Cost: ¥44,700,000); Fiscal Year 2005: ¥26,100,000 (Direct Cost: ¥26,100,000)
• Keywords: シグナル伝達 / 構造解析 / リガンド複合体 / インテグリン / 基底膜 / シグナル分子 / X線結晶構造解析 / 電子顕微鏡イメージング / 細胞外マトリックス / 受容体 / X線結晶解析 / 電子顕微鏡 / リーリン

Research Abstract

We have utilized X-ray crystallography and electron microscopy to decipher the structural mechanism underlying recognition of extracellular environmental cues by cellular receptors. We have gained information about reelin signal transduction in brain development and Wnt signal transduction implicated in cancer and bone formation. Also, we have unraveled the structure of neuronal synapse adhesion machinery, and succeeded in visualizing the rice dwarf virus during the infection in situ.

Research Products

• [Journal Article] Structural basis for specific recognition of reelin by its receptors. (2010)
  • Author(s): Yasui N, Nogi T, Takagi
  • Journal Title: Structure 18 Pages: 320-331
  • Peer Reviewed
• [Journal Article] Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation. (2010)
  • Author(s): Yasui N, Mihara E, Nampo M, Tamura-Kawakami K, Unno H, Matsumoto K, Takagi
  • Journal Title: J. immunol. Methods 352 Pages: 153-160
  • Peer Reviewed
• [Journal Article] Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation. (2010)
  • Author(s): Yasui, Norihisa
  • Journal Title: J.Immunol.Methods 352 Pages: 153-160
  • Peer Reviewed
• [Journal Article] Structural basis for specific recognition of reelin by its receptors. (2010)
  • Author(s): Yasui, Norihisa
  • Journal Title: Structure 18 Pages: 320-331
  • Peer Reviewed
• [Journal Article] Structural insights into von Willebrand factor cleavage by ADAMTS13. (2009)
  • Author(s): Akiyama, M., Takeda, S., Kokame, K., L Takagi, Miyata, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA (in press)
  • Peer Reviewed
• [Journal Article] Production, crystallization and preliminary crystallographic analysis of the exosite-containing fragment of human von Willebrand factor-cleaving proteinase, ADAMTS 13. (2009)
  • Author(s): Akiyama, M., Takeda, S., Kokame, K., L Takagi., Miyata, T.
  • Journal Title: Acta Crystallographica. F65 Pages: 739-742
  • Peer Reviewed
• [Journal Article] Association of Rice gall dwarf virus with microtubules is necessary for viral release from cultured insect vector cells (2009)
  • Author(s): T. Wei, T. Ichiki-Uehara, N. Miyazaki, H. Hibino, K. Iwasaki, T. Omura
  • Journal Title: J. Virol. 83(20) Pages: 10830-10835
  • Peer Reviewed
• [Journal Article] A self-assembled protein nanotubule with high aspect ratio (2009)
  • Author(s): Frederico F. Miranda, Kenji Iwasaki, Satoko, Akashi, Koji Sumitomo, Mime Kobayashi, Ichiro Yamashita, Jeremy R.H. Tamae, Jonathan G. Heddle.
  • Journal Title: Small. 5 Pages: 2077-2084
  • Peer Reviewed
• [Journal Article] The structure of rat liver vault at 3. 5 angstrom resolution, H. Tanaka, K. Kato, E. Yamashita (2009)
  • Author(s): H. Tanaka, K. Kato, E. Yamashita, T. Sumizawa, Y. Zhou, M. Yao K. Iwasaki, M. Yoshimura, T. Tsukihara
  • Journal Title: Science 323 Pages: 384-388
  • Peer Reviewed
• [Journal Article] Molecular basis of the recognition of nephronectin by integrin alpha8betal. (2009)
  • Author(s): Sato, Y., Uemura, T., Morimitsu, K., Sato-Nishiuchi, R., Manabe, R., Takagi, J., Yamada, M., Sekiguchi, K.
  • Journal Title: J. Biol. Chem. 284 Pages: 14524-14536
  • Peer Reviewed
• [Journal Article] The structure of rat liver vault at 3.5 angstrom resolution. (2009)
  • Author(s): Tanaka, Hideaki
  • Journal Title: Science 323 Pages: 384-388
  • Peer Reviewed
• [Journal Article] Molecular dynamism of Fe-S cluster biosynthesis implicated by the structure of SufC2-SufD2 complex. (2009)
  • Author(s): Wada, Kei
  • Journal Title: J.Mol.Biol. 387 Pages: 245-258
  • Peer Reviewed
• [Journal Article] Molecular basis of the recognition of nephronectin by integrin alpha8betal. (2009)
  • Author(s): Sato, Yuya
  • Journal Title: J.Biol.Chem. 284 Pages: 14524-14536
  • Peer Reviewed
• [Journal Article] Production, crystallization and preliminary crystallographic analysis of the exosite-containing fragment of human von Willebrand factor-cleaving proteinase, ADAMTS13. (2009)
  • Author(s): Akiyama, Masashi
  • Journal Title: Acta Crystallographica F65 Pages: 739-742
  • Peer Reviewed
• [Journal Article] Structural insights into von Willebrand factor cleavage by ADAMTS13. (2009)
  • Author(s): Akiyama, Masashi
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 19274-19279
  • Peer Reviewed
• [Journal Article] Structure of F-spondin reeler domain reveals a unique β-sandwich fold with a deformable disulfide-bonded loop. (2008)
  • Author(s): Nagae, M., Nishikawa, K., Yasui, N., Yamasaki, M., Nogi, T., Takagi, J.
  • Journal Title: Acta Crystallographica D64 Pages: 1138-1145
  • Peer Reviewed
• [Journal Article] Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification. (2008)
  • Author(s): Nogi, T., Sangawa, T., Tabata, S., Nagae, M., Tamura-Kawakami, K., Beppu, A., Hattori, M., Yasui, N., Takagi, J.
  • Journal Title: Protein Sci. 17 Pages: 2120-2126
  • Peer Reviewed
• [Journal Article] A murine monoclonal antibody that binds N-terminal extracellular segment of human protease activated receptor-4. (2008)
  • Author(s): Sangawa, T., Nogi, T., Takagi, J.
  • Journal Title: Hybridoma 27(5) Pages: 331-335
  • Peer Reviewed
• [Journal Article] Key interactions in ectodomain of integrin for global conformational change detected by elastic network normal mode analysis. (2008)
  • Author(s): Atsushi Matsumoto, Tetsuji Kamata, Junichi Takagi, Kenji Iwasaki, Kei Yura.
  • Journal Title: Biophys. J. 95(6) Pages: 2895-2908
  • Peer Reviewed
• [Journal Article] Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus. (2008)
  • Author(s): Yamaguchi, H., Takagi, J., Miyamae, T., Yokota, S., Fujimoto, T., Nakamura, S., Ohshima, S., Naka, T., Nagata, S.
  • Journal Title: J. Leukocyte Biol. 83 Pages: 1300-1307
  • Peer Reviewed
• [Journal Article] Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus (2008)
  • Author(s): Yamaguchi, Hiroshi
  • Journal Title: J. Leukocyte Biol. 83 Pages: 1300-1307
  • Peer Reviewed
• [Journal Article] Key interactions in ectodomain of integrin for global conformational change detected by elastic network normal mode analysis (2008)
  • Author(s): Matsumoto, Atsushi
  • Journal Title: Biophys. J. 95 Pages: 2895-2908
  • Peer Reviewed
• [Journal Article] Structure of F-spondin reeler domain reveals a unique b-sandwich fold with a deformable disutfide-bonded loop (2008)
  • Author(s): Nagae, Masamichi
  • Journal Title: Ada Crystallographica D 64 Pages: 1138-1145
  • Peer Reviewed
• [Journal Article] A murine monoclonal antibody that binds N-terminal extracellular segment of human protease activated receptor-4 (2008)
  • Author(s): Sangawa, Takeshi
  • Journal Title: Hybridoma 27 Pages: 331-335
  • Peer Reviewed
• [Journal Article] Novel affinity tag system using structurally defined antibody-tag interaction : Application to single-step protein purification (2008)
  • Author(s): Nogi, Terukazu
  • Journal Title: Protein Science 17 Pages: 2120-2126
  • Peer Reviewed
• [Journal Article] Pleomorphic Configuration of the Trimeric Capsid Proteins of Rice dwarf virus that Allows Formation of Both the Outer Capsid and Tubular Crystals (2008)
  • Author(s): Iwasaki, Kenji
  • Journal Title: J. Mol. Biol. 383 Pages: 252-265
  • Peer Reviewed
• [Journal Article] A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry (2008)
  • Author(s): Kato, Koji
  • Journal Title: Ada Crystallographica D 64 Pages: 525-531
  • Peer Reviewed
• [Journal Article] Structural basis for ligand recognition by integrins. (2007)
  • Author(s): Takagi, J.
  • Journal Title: Curr. Opin. Cell Biol. 19 Pages: 557-564
• [Journal Article] receptor-binding reelin fragment and mutational analysis reveal a recognition mechanism similar to endocytic receptors. (2007)
  • Author(s): Yasui, N., Nogi, T., Kitao, T., Nakano, Y., Hattori, M., Takagi, J.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 9988-9993
  • Peer Reviewed
• [Journal Article] The RGD motif in fibronectin is essential for mouse development but dispensable for fibronectin fibril assembly. (2007)
  • Author(s): Takahashi, S., Leiss, M., Moser, M., Ohashi, T., Kitao, T., Heckmann, D., Pfeifer, A., Kessler, H., Takagi, T., Erickson, H.P., Fassler, R.
  • Journal Title: J. Cell Biol. 178 Pages: 167-178
  • Peer Reviewed
• [Journal Article] Three-dimensional architecture of virus-packed tubule. (2007)
  • Author(s): Katayama, S., Wei, T., Omura, T., Takagi., Iwasaki, K.
  • Journal Title: J. Electron Microsc. 56 Pages: 77-81
  • NAID: 10028200118
  • Peer Reviewed
• [Journal Article] Semaphorin 7A initiates T cell-mediated inflammatory responses through a1131 integrin. (2007)
  • Author(s): Suzuki K., Okuno, T., Yamamoto, M., Pasterkamp, R.J., Takegahara, N., Kitao, T., Takagi, J., Rennert, P.D., Kolodkin, A.L., Kumanogoh, A., Kikutani, H.
  • Journal Title: Nature 446 Pages: 680-684
  • Peer Reviewed
• [Journal Article] aVr36 is a novel receptor for human fibrillin-1: comparative studies of molecular determinants underlying integrin-RGD affinity and specificity. (2007)
  • Author(s): Jovanovic J, Takagi J, Choulier L, Abrescia NG, Stuart DI, van der Merwe PA, Mardon HJ, Handford PA
  • Journal Title: J. Biol. Chem. 282 Pages: 6743-6751
  • Peer Reviewed
• [Journal Article] Semaphorin 7A initiates T cell-mediated inflammatory responses through a1b1 integrin. (2007)
  • Author(s): Suzuki, Kazuhiro
  • Journal Title: Nature 446 Pages: 680-684
  • Peer Reviewed
• [Journal Article] Three-dimensional architecture of virus-packed tubule. (2007)
  • Author(s): Katayama, Sumie
  • Journal Title: J. Electron Microsc 56 Pages: 77-81
  • Peer Reviewed
• [Journal Article] The RGD motif in fibronectin is essential for mouse development but dispensable for fibronectin fibril assembly. (2007)
  • Author(s): Takahashi, Seiichiro
  • Journal Title: J. Cell Biol. 178 Pages: 167-178
  • Peer Reviewed
• [Journal Article] Structure of a receptor-binding reelin fragment and mutational analysis reveal a recognition mechanism similar to endocytic receptors. (2007)
  • Author(s): Yasui, Norihisa
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 9988-9993
  • Peer Reviewed
• [Journal Article] Structural basis for ligand recognition by integrins. (2007)
  • Author(s): Takagi, Junichi
  • Journal Title: Curr. Opin. Cell Biol. 19 Pages: 557-564
  • Peer Reviewed
• [Journal Article] Alpha Vbeta 6 is a novel receptor for human fibrillin-1 : comparative studies of molecular determinants underlying integrin-RGD affinity and specificity. (2007)
  • Author(s): Jovanovic, Jelena
  • Journal Title: J. Biol. Chem. 282 Pages: 6743-6751
• [Journal Article] Structure of a signaling-competent reelin fragment revealed by X-ray crystallography and electron tomography. (2006)
  • Author(s): Nogi, T., Yasui, N., Hattori, M., Iwasaki, K., Takagi, J.
  • Journal Title: EMBO J. 25 Pages: 3675-3683
  • Peer Reviewed
• [Journal Article] モジュラータンパク質の構造解析:受容体構造生物学における中心課題 (2006)
  • Author(s): 高木淳一
  • Journal Title: 日本結晶学会誌 48 Pages: 411-417
  • NAID: 10018467400
• [Journal Article] Structure of a signaling-competent reelin fragment revealed by X-ray crystallography and electron tomography. (2006)
  • Author(s): Nogi, Terukazu
  • Journal Title: EMBO J. 25 Pages: 3675-3683
• [Journal Article] モジュラータンパク質の構造解析 : 受容体構造生物学における中心課題 (2006)
  • Author(s): 高木, 淳一
  • Journal Title: 日本結晶学会誌 48 Pages: 411-417
  • NAID: 10018467400
• [Journal Article] Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin alpha v beta 3 induces signal transduction and enhances plasminogen activation. (2006)
  • Author(s): Tarui, Takechiko
  • Journal Title: Thromb. Heamost. 95 Pages: 524-534
• [Journal Article] Ligand-binding specificities of laminin-binding integrins : a comprehensive survey of laminin-integrin interactions using recombinant alpha3betal, alpha6betal, alpha7betal and alpha6beta4 integrins. (2006)
  • Author(s): Nishiuchi, Ryoko
  • Journal Title: Matrix Biology 25 Pages: 189-197
• [Journal Article] Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering. (2005)
  • Author(s): Luo, B.H., Carman, C.V., Takagi, J., Springer, T.A.
  • Journal Title: Proc Natl Acad Sci U S A 102 Pages: 3679-3684
  • Peer Reviewed
• [Journal Article] Potentiation of the ligand-binding activity of integrin alpha3 betal via association with tetraspanin CD151. (2005)
  • Author(s): Nishiuchi, R., Sanzen, N., Nada, S., Sumida, Y., Wada, Y., Okada, M., Takagi, J., Hasegawa, H., Sekiguchi, K.
  • Journal Title: Proc Natl Acad Sci U S A 102 Pages: 1939-1944
  • Peer Reviewed
• [Journal Article] 電子顕微鏡を用いた蛋白質の高次構造解析が開く世界-インテグリンレセプターの構造解析を中心として- (2005)
  • Author(s): 岩崎憲治、高木淳一
  • Journal Title: 生化学 77 Pages: 400-410
• [Journal Article] Potentiation of the ligand-binding activity of integrin alpha3 beta1 via association with tetraspanin CD151 (2005)
  • Author(s): Nishiuchi, Ryoko
  • Journal Title: Proc Natl Acad Sci USA 102 Pages: 1939-1944
• [Journal Article] Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering (2005)
  • Author(s): Luo, Bing-hao
  • Journal Title: Proc Natl Acad Sci USA 102 Pages: 3679-3684
• [Journal Article] レセプター膜貫通ドメインの構造とそのシグナリングにおける動的制御 (2005)
  • Author(s): 高木 淳一
  • Journal Title: 蛋白質核酸酵素 50(10) Pages: 1226-1232
• [Journal Article] 電子顕微鏡を用いた蛋白質の高次構造解析が開く世界-インテグリンレセプターの構造解析を中心として- (2005)
  • Author(s): 岩崎 憲治
  • Journal Title: 生化学 77 Pages: 400-410
• [Presentation] ニューレキシン・ニューロリギン複合体の結晶構造に基づくシナプス形成機構の構造生物学的解析 (2010)
  • Author(s): 禾、晃和
  • Organizer: 第27回PFシンポジウム
  • Place of Presentation: つくば国際会議場エポカル
  • Year and Date: 2010-03-09
• [Presentation] 神経シナプスの形成を制御するニューレキシン・ニューロリギン複合体の結晶構造 (2010)
  • Author(s): 禾晃和
  • Organizer: 第23回日本放射光学会年会放射光科学合同シンポジウム
  • Place of Presentation: 姫路
  • Year and Date: 2010-01-08
• [Presentation] 神経シナプスの形成を綱御するニューレキシン・ニューロリギン複合体の結晶構造 (2010)
  • Author(s): 禾、晃和
  • Organizer: 第23回日本放射光学会年会放射光科学合同シンポジウム
  • Place of Presentation: イーグレひめじ
  • Year and Date: 2010-01-08
• [Presentation] EM imaging of Rice Dwarf Virus (2010)
  • Author(s): Iwasaki, K.
  • Organizer: China-Japan 3D-EM Forum
  • Place of Presentation: Beijing(中国)
  • Year and Date: 2010-01-05
• [Presentation] EM imaging of Rice Dwarf Virus (2010)
  • Author(s): Iwasaki, Kenji
  • Organizer: China-Japan 3D-EM Forum
  • Place of Presentation: The Conference Hall of the Institute of Biophysics, Beijing(中国)
  • Year and Date: 2010-01-05
• [Presentation] シナプス間接着蛋白質ニューレキシン・ニューロリギンの接着機構の構造的基盤 (2009)
  • Author(s): 田中、宏樹
  • Organizer: 特定領域研究「生体超分子構造」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2009-12-02
• [Presentation] シナプス間接着分子Neuroligin/Neurexin複合体の構造から見えるシナプス間隙の真の姿 (2009)
  • Author(s): 高木淳一
  • Organizer: 第5回プロテオミクス・構造生物学講演会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-22
• [Presentation] シナプス間接着分子Neuroligin/Neurexin複合体の構造から見えるシナプス間隙の真の姿 (2009)
  • Author(s): 高木、淳一
  • Organizer: 第5回プロテオミクス・構造生物学講演会
  • Place of Presentation: 学術総合センター
  • Year and Date: 2009-11-22
• [Presentation] Rice gall dwarf virusは、マイクロチュープルを介して昆虫細胞内を移行し細胞外に放出される (2009)
  • Author(s): 一木(植原)、珠樹
  • Organizer: 第57回日本ウィルス学会学術集会
  • Place of Presentation: 都市センターホテル
  • Year and Date: 2009-10-26
• [Presentation] リーリン会合体の構造的基盤とその機能的意義 (2009)
  • Author(s): 安井典久
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] リーリン会合体の構造的基盤とその機能的意義 (2009)
  • Author(s): 安井、典久
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ボートアイランド
  • Year and Date: 2009-10-24
• [Presentation] LR11 Vps10pドメインの構造および機能解析 (2009)
  • Author(s): 中田、善三郎
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] Dissection of the molecular architecture of higher-order assembly of the full-length reelin (2009)
  • Author(s): Yasui, N.
  • Organizer: 23rd Symposium of The Protein Society
  • Place of Presentation: Boston(USA)
  • Year and Date: 2009-07-28
• [Presentation] Dissection of the molecular architecture of higher-order assembly of the full-length reelin (2009)
  • Author(s): Norihisa, Yasui
  • Organizer: 23rd Symposium of The Protein Society
  • Place of Presentation: Boston Marriott Copley Place, Boston (米国)
  • Year and Date: 2009-07-28
• [Presentation] Crystal structure of synaptic adhesion complex; insights into the molecular assembly at the synaptic membrane (2009)
  • Author(s): Takagi, J.
  • Organizer: 23rd Symposium of The Protein Society
  • Place of Presentation: Boston(USA)
  • Year and Date: 2009-07-27
• [Presentation] Crystal structure of synaptic adhesion complex ; insights into the molecular assembly at the synaptic membrane (2009)
  • Author(s): Takagi, Junichi
  • Organizer: 23rd Symposium of The Protein Society
  • Place of Presentation: Boston Marriott Copley Place, Boston (米国)
  • Year and Date: 2009-07-27
• [Presentation] Electron microscopy study of platelet and integrin using new methods (2009)
  • Author(s): Iwasaki, K.
  • Organizer: 第32回日本血栓止血学会学術集会
  • Place of Presentation: 小倉
  • Year and Date: 2009-06-04
• [Presentation] Electron microscopy study of platelet and integrin using new methods (2009)
  • Author(s): 岩崎、憲治
  • Organizer: 第32回日本血栓止血学会学術集会
  • Place of Presentation: リーガロイヤルホテル小倉
  • Year and Date: 2009-06-04
• [Presentation] CEMOVISの評価とクライオ電子線トモグラフィー (2009)
  • Author(s): 岩崎憲治
  • Organizer: 日本顕微鏡学会第65回学術講演会
  • Place of Presentation: 仙台
  • Year and Date: 2009-05-27
• [Presentation] CEMOVISの評価とクライオ電子線トモグラフィー (2009)
  • Author(s): 岩崎、憲治
  • Organizer: 日本顕微鏡学会第65回学術講演会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2009-05-27
• [Presentation] 全長リーリンの精製法の確立とリーリン会合体の構造化学的基盤の解明 (2009)
  • Author(s): 安井典久
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2009-05-22
• [Presentation] 全長リーリンの精製法の確立とリーリン会合体の構造化学的基盤の解明 (2009)
  • Author(s): 安井、典久
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本全日空ホテルニュースカイ
  • Year and Date: 2009-05-22
• [Presentation] シナプス間接着装置ニューレキシン-ニューロリギン複合体の分子間クラスタリングの構造基盤 (2009)
  • Author(s): 田中、宏樹
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本全日空ホテルニュースカイ
  • Year and Date: 2009-05-20
• [Presentation] Novel affinity tag system using structurally defined antibody-tag interaction-Application to single-step protein purification- (2009)
  • Author(s): Takagi, Junichi
  • Organizer: PepTalk2009
  • Place of Presentation: San Diego, Hotel del Coronado
  • Year and Date: 2009-01-12
• [Presentation] Affinity tag peptide with a built-in TEV protease recognition sequence (2009)
  • Author(s): Takagi, Junichi
  • Organizer: The Bioprocessing Summit
  • Place of Presentation: Boston, Royal Sonesta Hotel (米国)
• [Presentation] クライオ電子顕微鏡法を用いた活性化血小板の分子分解能構造解析 (2008)
  • Author(s): 片山寿美枝
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] クライオ電子顕微鏡法を用いた活性化血小板の分子分解能構造解析 (2008)
  • Author(s): 片山、寿美枝
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-10
• [Presentation] クライオ電子顕微鏡法を用いた活性化血小板の分子分解能構造解析 (2008)
  • Author(s): 岩崎、憲治
  • Organizer: 第31回日本血栓止血学会学術集会
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2008-11-20
• [Presentation] Cryo-Electron Tomography for Single Particles (2008)
  • Author(s): Iwasaki, K.
  • Organizer: 9th Asia-Pacific Microscopy Conference
  • Place of Presentation: Jeju (Korea)
  • Year and Date: 2008-11-02
• [Presentation] Cryo-Electron Tomography for Single Particles (2008)
  • Author(s): Iwasaki, Kenji
  • Organizer: 9th Asia-Pacific Microscopy Conference
  • Place of Presentation: ICC Jeju, Jeju, Korea
  • Year and Date: 2008-11-02
• [Presentation] シナプス問接着分子Neurexin/Neuroligin複合体の構造から見えるシナプス間隙の真の姿 (2008)
  • Author(s): 高木、淳一
  • Organizer: 学振第169委員会第27会研究会
  • Place of Presentation: 京都大学百周年時計台記念館
  • Year and Date: 2008-10-28
• [Presentation] Crystal structure ofsynaptic adhesion protein neurexin and neuroligin (2008)
  • Author(s): Tanaka, H.
  • Organizer: XXI Congress of the International Union of Crystallography
  • Place of Presentation: Osaka
  • Year and Date: 2008-08-23
• [Presentation] Crystal structure ofsynaptic adhesion protein neurexin and neuroligin (2008)
  • Author(s): Tanaka, Hiroki
  • Organizer: XXI Congress of the International Union of Crystallo graphy
  • Place of Presentation: Grand Cube Osaka, Osaka, Japan
  • Year and Date: 2008-08-23
• [Presentation] Crystal structure of reelin in complex with its receptor (2008)
  • Author(s): Yasui, Norihisa
  • Organizer: XXI Congress of the International Union of Crystallo graphy
  • Place of Presentation: Grand Cube Osaka, Osaka, Japan
  • Year and Date: 2008-08-23
• [Presentation] Structures of the laminin-binding integrint (2008)
  • Author(s): Iwasaki, Kenji
  • Organizer: XXI Congress of the International Union of Crystallo graphy
  • Place of Presentation: Grand Cube Osaka, Osaka, Japan
  • Year and Date: 2008-08-23
• [Presentation] Structural basis for recognition of reelin by its neuronal receptor (2008)
  • Author(s): Yasui, N.
  • Organizer: 22nd Annual Symposium of The Protein Society
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2008-07-29
• [Presentation] Structural basis for recognition of reelin by its neuronal receptor (2008)
  • Author(s): Yasui, Norihisa
  • Organizer: 22nd Annual Symposium of The Protein Society
  • Place of Presentation: San Diego, Manchester Grand Hyatt San Diego
  • Year and Date: 2008-07-29
• [Presentation] Development of a novel affinity tag system for the next generation structural biology (2008)
  • Author(s): Takagi, Junichi
  • Organizer: 2nd Pacific-Rim International Conference on Protein Science
  • Place of Presentation: Cairns Convention Center, Cairns. Australia
  • Year and Date: 2008-06-22
• [Presentation] リーリン工受容体複合体の結晶構造解析 (2008)
  • Author(s): 安井典久
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-10
• [Presentation] F-spondinリーラードメインのX線結晶構造解析 (2008)
  • Author(s): 長江雅倫
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-10
• [Presentation] リーリン-受容体複合体の結晶構造解析 (2008)
  • Author(s): 安井、典久
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2008-06-10
• [Presentation] シナプス間接着蛋白質ニューレキシン-ニューロリギンの構造に基づく接着機構の解明 (2008)
  • Author(s): 田中、宏樹
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2008-06-10
• [Presentation] F-spondinリーラードメインのX線結晶構造解析 (2008)
  • Author(s): 長江、雅倫
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2008-06-10
• [Presentation] EM Navigator 3次元電子顕微鏡データブラウザ (2008)
  • Author(s): 岩崎、憲治
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2008-06-10
• [Presentation] 電子顕微鏡イメージングによるラミニン結合型インテグリンの構造解析 (2008)
  • Author(s): 宮崎、直幸
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2008-06-10
• [Presentation] 脳の層構造形成を司る細胞外因子リーリンの結品構造と受容体による認識機構 (2007)
  • Author(s): 高木淳一
  • Organizer: 日本分子生物学会・日本生化学会合同シンポジウム(BMB2007)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-15
• [Presentation] 脳の層構造形成を司る細胞外因子リーリンの結晶構造と受容体による認識機構 (2007)
  • Author(s): 高木、淳一
  • Organizer: 日本分子生物学会・日本生化学会合同シンポジウム (BMB2007)
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-15
• [Presentation] シナプス間接着タンパク質αニューレキシンの細胞外ドメインの構造解析 (2007)
  • Author(s): 田中宏樹
  • Organizer: 日本結晶学会2007年度年会
  • Place of Presentation: 東京
  • Year and Date: 2007-12-02
• [Presentation] シナプス間接着タンパク質αニューレキシンの細胞外ドメインの構造解析 (2007)
  • Author(s): 田中、宏樹
  • Organizer: 日本結晶学会2007年度年会
  • Place of Presentation: 東京工業大学
  • Year and Date: 2007-12-02
• [Presentation] Challenges in structural analysis of extracellular protein complexes (2007)
  • Author(s): Takagi.
  • Organizer: 2nd International Symposium on Diffraction Structural Biology 2007
  • Place of Presentation: Tokyo
  • Year and Date: 2007-09-11
• [Presentation] Challenges in structural analysis of extracellular protein complexes (2007)
  • Author(s): Takagi, Junichi
  • Organizer: 2nd Intemational Symposium on Diffraction Structural Biology 2007
  • Place of Presentation: Tokyo
  • Year and Date: 2007-09-11
• [Presentation] Crystal Structure of a Signaling- competent Reelin Fragment (2007)
  • Author(s): Nogi, T.
  • Organizer: The 2007 Meeting of the American Crystallographic Association
  • Place of Presentation: Salt Lake City(USA)
  • Year and Date: 2007-07-25
• [Presentation] Crystal Structure of a Signaling- competent Reelin Fragment (2007)
  • Author(s): Nogi, Terukazu
  • Organizer: The 2007 Meeting of the American Crystallographic Association
  • Place of Presentation: Salt Lake City
  • Year and Date: 2007-07-25
• [Presentation] 受容体によるリーリン認識の構造的基盤 (2007)
  • Author(s): 安井典久
  • Organizer: 第7回日本蛋白質科学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-25
• [Presentation] 受容体によるリーリン認識の構造的基盤 (2007)
  • Author(s): 安井、典久
  • Organizer: 第7回日本蛋白質科学会年会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2007-05-25
• [Presentation] シナプス間接着蛋白質αニューレキシンの細胞外ドメインの構造解析 (2007)
  • Author(s): 田中、宏樹
  • Organizer: 第7回日本蛋白質科学会年会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2007-05-25
• [Presentation] 抗GPCRモノクローナル抗体20.1を用いたアフィニティータグシステムの開発I-体作成とエピトープ解析 (2007)
  • Author(s): 寒川、剛
  • Organizer: 第7回日本蛋白質科学会年会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2007-05-25
• [Presentation] 抗GPCRモノクローナル抗体20.1を用いたアフィニティータグシステムの開発II-Fabフラグメントの結晶構造解析 (2007)
  • Author(s): 禾、晃和
  • Organizer: 第7回日本蛋白質科学会年会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2007-05-25
• [Presentation] 抗GPCR抗体20.1を用いたアフィニティータグシステムの開発 III-高親和性ペプチド配列の探索 (2007)
  • Author(s): 田畑、早苗
  • Organizer: 第7回日本蛋白質科学会年会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2007-05-25
• [Presentation] 電子線トモグラフィー・単粒子解析・X線結晶構造解析によるハイブリッド構造解析 (2007)
  • Author(s): 岩崎、憲治
  • Organizer: 日本顕微鏡学会第63回学術講演会
  • Place of Presentation: 朱鷺メッセ(新潟)
• [Presentation] A new imaging method using electron beam tomography (2007)
  • Author(s): 岩崎、憲治
  • Organizer: 化学血清療法研究所主催第5回Aso International Meeting
  • Place of Presentation: ホテルグリーンピア南阿蘇
• [Presentation] クライオ3D:マクロ分子の構造解析 (2007)
  • Author(s): 岩崎、憲治
  • Organizer: 日本顕微鏡学会第63回学術講演会
  • Place of Presentation: 朱鷺メッセ(新潟)
• [Presentation] 電子線トモグラフィー (2007)
  • Author(s): 岩崎、憲治
  • Organizer: 第7回日本蛋白質科学会年会
  • Place of Presentation: 仙台国際センター(仙台)
• [Presentation] 電子顕微鏡イメージング法の現状と未来 (2007)
  • Author(s): 岩崎、憲治
  • Organizer: 第4回放射光学会若手ワークショップ
  • Place of Presentation: Spring-8放射光普及棟
• [Presentation] インテグリンファミリーにおける特異的な構造の探索 (2007)
  • Author(s): 宮崎、直幸
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会 合同大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 3次元電子断層撮影法のためのGUIツールの開発 (2007)
  • Author(s): 後藤、龍伯
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: パシフィコ横浜
• [Book] インテグリン、「ますます重要になる細胞周辺環境(細胞ニッチ)の最新科学技術」(田畑泰彦編) (2009)
  • Author(s): 高木淳一
  • Publisher: ディカルドゥ
• [Book] Reelin Glycoprotein, biology, structure and roles in health and disease (2008)
  • Author(s): Takagi, J.
  • Publisher: Springer
• [Book] Reelin Glycoprotein, biology, structure and roles in health and disease (2008)
  • Author(s): Takagi,Junichi.(分担)
  • Total Pages: 444
  • Publisher: Springer
• [Book] ポストゲノム時代のタンパク質科学-構造・機能・ゲノミクス- (Arthur M. Lesk原著) (2007)
  • Author(s): 高木淳一
  • Publisher: 化学同人
• [Book] 再生医療のための細胞生物学(関口清俊編) (2007)
  • Author(s): 高木淳一
  • Publisher: コロナ社
• [Book] ポストゲノム時代のタンパク質科学-構造・機能・ゲノミクスー (2007)
  • Author(s): 高木、淳一
  • Total Pages: 328
  • Publisher: 化学同人
• [Book] 再生医療のための細胞生物学(関口清俊 編) (2006)
  • Author(s): 高木, 淳一(分担)
  • Total Pages: 176
  • Publisher: コロナ社
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/rcsfp/synthesis/index.html
• [Patent(Industrial Property Rights)] プロテアーゼ認識配列を有するタグペプチドおよびその利用 (2009)
  • Inventor(s): 高木淳一
  • Industrial Property Rights Holder: 国立大学法人大阪大学
  • Industrial Property Number: 2009-103925
  • Filing Date: 2009-04-22
• [Patent(Industrial Property Rights)] タグペプチド及びその利用 (2008)
  • Inventor(s): 高木淳一
  • Industrial Property Rights Holder: 国立大学法人大阪大学
  • Filing Date: 2008-12-18
  • Overseas
• [Patent(Industrial Property Rights)] タグペプチドが結合した融合タンパク質、タグペプチドに対する抗体及びこれらを用いるタンパク質の精製方法 (2008)
  • Inventor(s): 高木淳一
  • Industrial Property Rights Holder: 国立大学法人大阪大学
  • Industrial Property Number: 2008-020804
  • Filing Date: 2008-01-31