Molecular analysis of pathogenesis on Sjogren's syndrome and its application of new diagnosis and therapy

• Project/Area Number: 17109016
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: The University of Tokushima
• Principal Investigator: HAYASHI Yoshio The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 教授 (00127854)
• Co-Investigator(Kenkyū-buntansha): ISHIMARU Naozumi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (60314879) ; ARAKAKI Rieko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (00193061) ; 新垣 理恵子 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (40231120)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥96,850,000 (Direct Cost: ¥74,500,000、Indirect Cost: ¥22,350,000); Fiscal Year 2008: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000); Fiscal Year 2007: ¥21,840,000 (Direct Cost: ¥16,800,000、Indirect Cost: ¥5,040,000); Fiscal Year 2006: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000); Fiscal Year 2005: ¥35,100,000 (Direct Cost: ¥27,000,000、Indirect Cost: ¥8,100,000)
• Keywords: シェーグレン症候群 / 唾液腺 / 自己抗原 / α-フォドリン / アポトーシス / システインプロテアーゼ / トレランス / 疾患モデル / RbAp48 / RANKL / Fas / クラスII分子 / IFN-γ / 抹消トレランス / エストロゲン / 性差 / p53 / チトクロムc / 自己免疫疾患

Research Abstract

シェーグレン症候群を含めた自己免疫疾患の大半は原因不明であり疾患非特異的な診断・治療法で対処しているのが世界の現状である。本研究プロジェクトは難治性自己免疫疾患シェーグレン症候群の発症に密接に関連するアポトーシス経路を介した膜タンパク破綻の分子基盤の全容解明による新たな診断・治療法の創出を目的として実施した。

Research Products

• [Journal Article] Neonatal exposure to low-dose 2, 3, 7, 8-tetrachlorcdibenzo-p-dioxin causes autoimmunity due to the disruption of T cell tolerance (2009)
  • Author(s): Ishimaru N, Takaji A, Kohashi M,Yamada A, Arakaki R, Kanno J, Hayashi Y
  • Journal Title: J. Immunol 182 Pages: 6576-6586
  • Peer Reviewed
• [Journal Article] Role of plasmacytoid dendritic cells for aberrant class II expression in exocrine glands from estrogen-deficient mice of healty background (2009)
  • Author(s): Arakaki R, Nagaoka A, Ishimaru N, Yamada A, Yoshida S, Hayashi Y
  • Journal Title: Am. j. Pathol 174 Pages: 1715-1715
  • Peer Reviewed
• [Journal Article] Expression of the retinoblastoma protein RbAp48 in exocrine glands leads to Sjogren' s syndrome-like autoimmune exocrinopathy (2009)
  • Author(s): Ishimaru N, Arakaki R, Yoshida S, Yamada A, Noji S, Hayashi Y
  • Journal Title: J. Exp. Med 205 Pages: 2915-2927
  • Peer Reviewed
• [Journal Article] Development of inflammatory bowel disease in Long-Evans Cinnamon rats based on CD4^+CD25^+Foxp3^+ regulatory T cell dysfunction (2008)
  • Author(s): Ishimaru N, Yamada A, Kohashi M, Arakaki R Takahashi T, Izumi K, Hayashi Y
  • Journal Title: J. Immunol 180 Pages: 6997-7008
  • Peer Reviewed
• [Journal Article] Effective treatment of a mouse model of Sjogren' s syndrome with oral administration of rebamipide (2008)
  • Author(s): Kohashi M, Ishimaru N, Arakaki R ; Hayashi Y
  • Journal Title: Arthritis Rheum 58 Pages: 389-400
  • Peer Reviewed
• [Journal Article] Development of inflammatory bowel disease (IBD) in Long-Evans Cinnamon (LEC) rats based on CD4^+CD25^+Foxp3^+ regulatory T cell dysfunction. (2008)
  • Author(s): Ishimaru N., et al.
  • Journal Title: J Immunol 180 Pages: 6997-7008
  • Peer Reviewed
• [Journal Article] RANKL produced by positively selected thymocytes fosters medullary thymic epithelial cells that express autoimmune regulator. (2008)
  • Author(s): Hikosaka Y., et al.
  • Journal Title: Immunity 29 Pages: 438-450
  • Peer Reviewed
• [Journal Article] Expression of the retinoblastoma protein RbAp48 in exocrine glands leads to Sjogren's syndrome-like autoimmune exocrinopathy. (2008)
  • Author(s): Ishimaru N., et al
  • Journal Title: J Exp Med 205 Pages: 2915-29270
  • Peer Reviewed
• [Journal Article] Development of inflammatory bowel in Long-Evance Cinnamon(LEC)rats based on CD4+CD25+Foxp3+ regulatory T cell dysfunction (2008)
  • Author(s): Ishimaru N.
  • Journal Title: J. Immunol 180 Pages: 6997-7008
  • Peer Reviewed
• [Journal Article] Atelocollagen-mediated local and systemic applications of myostatin-targeting siRNA increase skeletal muscle mass (2008)
  • Author(s): Kinouchi N.
  • Journal Title: Gene Ther. in press(advance online)
  • Peer Reviewed
• [Journal Article] Epitope mapping of anti-・-fodrin antibody in a case of early-onset multiple sclerosis (2008)
  • Author(s): Okura Y.
  • Journal Title: Pediatrics Int. 50 Pages: 135-137
  • Peer Reviewed
• [Journal Article] Effective treatment of a mouse model of Sjogren's syndrome with oral administration of rebamipide (2008)
  • Author(s): Kohashi M.
  • Journal Title: Arthritis Rheum. 58 Pages: 389-400
  • Peer Reviewed
• [Journal Article] Crosstalk between RANKL and Fas signaling in dendritic cells controls immune tolerance (2007)
  • Author(s): Izawa T, Ishimaru N, Moriyama K, Kohashi M, Arakaki R, Hayashi Y
  • Journal Title: Blood 1110 Pages: 242-250
  • NAID: 40016915514
  • Peer Reviewed
• [Journal Article] Visualizing the dynamics of p21(Waf1/Cip1)cyclin-dependent kinase inhibitor expression in living animals (2007)
  • Author(s): Ohtani N.
  • Journal Title: Proc. Nat1. Acad. Sci. USA. 104 Pages: 15034-15039
  • Peer Reviewed
• [Journal Article] Crosstalk between RANKL and Fas signaling in dendritic cells controls immune tolerance (2007)
  • Author(s): Izawa T.
  • Journal Title: Blood 110 Pages: 242-250
  • Peer Reviewed
• [Journal Article] T細胞レセプターシグナルとNF-κB (2007)
  • Author(s): 石丸 直澄
  • Journal Title: 臨床免疫・アレルギー科 48 Pages: 29-35
  • Peer Reviewed
• [Journal Article] Crosstalk between RANKL and Fas signaling in dendritic cells controls immune tolerance (2007)
  • Author(s): Izawa T.
  • Journal Title: Blood (in press)
• [Journal Article] Regulation of nave T cell function by the NF-kB2 pathway (2006)
  • Author(s): Ishimaru N, Kishimoto H, Hayashi Y, Sprent J
  • Journal Title: Nature Immunol 7 Pages: 763-771
  • Peer Reviewed
• [Journal Article] Novel role of RbAp48 for tissue-specific. estrogen deficiency-dependent apoptosis in the exocrine glands (2006)
  • Author(s): Ishimaru N, Arakaki R, Omotehara F, Yamada K, Mishima K, Saito I, Hayashi Y
  • Journal Title: Mol. Cell. Biol 26 Pages: 2924-2935
  • Peer Reviewed
• [Journal Article] CCR7-dependent cortex-to medulla migration of positively selected thymocytes is essential for establishing central tolerance (2006)
  • Author(s): Kurobe H, Cunlan Liu C, UenoT, Saito F, Ohigashi I, Seach N, Arakaki R, Hayashi Y, Kitagawa T, Lipp M, Richard L. Boyd R, Takahama Y
  • Journal Title: Immunity 24 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Regulation of naive T cell function by the NF-kB2 pathway. (2006)
  • Author(s): Ishimaru N.
  • Journal Title: Nature Immunol 7 Pages: 763-771
• [Journal Article] CCR7-dependent cortex-to-medulla migration of positively selected thymocytes is dispensable for T-cell maturation and thymic export but is essential for establishing central tolerance. (2006)
  • Author(s): Kurobe H.
  • Journal Title: Immunity 24 Pages: 165-177
• [Journal Article] Ubiquitin ligase Cbl-b down-regulates bone formation through suppression of IGF-1 signaling in osteoblasts during denavation. (2006)
  • Author(s): Suzue N.
  • Journal Title: J.Bone Miner.Res. 21 Pages: 722-734
• [Journal Article] Catechin : Specific inhibitor for caspase-3, 7, and 2, and the prevention of apoptosis at the cell and animal levels (2006)
  • Author(s): Katunuma N.
  • Journal Title: FEBS Let 580 Pages: 741-746
• [Journal Article] Epitope mapping of anti-α-fodrin autoantibody in juvenile Siogren's syndrome : epitope spreading and difference in major epitope between primary and secondary cases. (2006)
  • Author(s): Shiari R.
  • Journal Title: J.Rheumatol. 33 Pages: 722-734
• [Journal Article] A novel role for RBAp48 for tissue-specific, estrogen-deficiency dependent apoptosis in the exocrine glands. (2006)
  • Author(s): Ishimaru N
  • Journal Title: Molecular Cellular Biology (in press)
• [Journal Article] Catechin : Specific inhibitor for caspase-3,7, and 2, and the prevention of apoptosis at the cell and animal levels. (2006)
  • Author(s): Katsunuma N
  • Journal Title: FEBS Letter (in press)
• [Journal Article] CCR7-dependent cortex-to-medulla migration of positively selected thymocytes is dispensable for T-cell maturation and thymic export but is essential for establishing central tolerance. (2006)
  • Author(s): Kurobe H
  • Journal Title: Immunity 24 Pages: 165-177
• [Journal Article] Mutations in the fibroblast growth factor 10 gene are associated with aplasia of lacrimal' and salivary glands (ALSG) (2005)
  • Author(s): Entesarian M, Matsson H, Klar J, Bergendal B, Olson L, Arakaki R, Hayashi Y, Ohuchi H, Falhat B, Bolstad AI, Jonsson R, Wahren-Herlenius M, Dahl N
  • Journal Title: Nature Genetics 37 Pages: 125-127
  • Peer Reviewed
• [Journal Article] Mutations in the fibroblast growth factor 10 gene are associated with plasia of lacrimal- and salivary glands (ALSG). (2005)
  • Author(s): Entesarian M
  • Journal Title: Nature Genetics 37 Pages: 125-127
• [Journal Article] Analysis of in vivo role of α-fodrin autoantigen in primary Sjogren's syndrome. (2005)
  • Author(s): Miyazaki K
  • Journal Title: American Journal of Pathology 167 Pages: 1051-1059
• [Journal Article] Development of autoimmunity against transcriptionally unrepressed target antigen in the thymus of Aire-deficient mice. (2005)
  • Author(s): Kuroda N
  • Journal Title: Journal of Immunology 174 Pages: 1862-1870
• [Presentation] Neonatal exposure to low-dose 2, 3, 4, 7, 8-tetrachloro-dibenzo---p-dioxin (TCDD) causes autoimmunity due to the disruption of T cell tolerance (2008)
  • Author(s): Ishimaru N. et.al.
  • Organizer: 第38回免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Identification and functional analysis of CDI1c+E220+dendritic clls induced by estrogen deficiency in salivaryglands (2008)
  • Author(s): Arakaki R. et.al.
  • Organizer: 第38回に本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Therapeutic analysis for autoimmune diabetes in NOD mice using specific inhibitor for cathepsin L. (2008)
  • Author(s): Yamada A., et al
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Identification and functional analysis of CD11c+B220+dendritic cells induced by estrogen deficiency in salivary glands. (2008)
  • Author(s): Arakaki R
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] 外分泌腺における異所性MHC class II発現に対するエストロゲン欠乏の役割 (2008)
  • Author(s): 新垣 理恵子
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: グランドプリンスホテル新高輪・品川(東京)
  • Year and Date: 2008-11-22
• [Presentation] エストロゲン欠乏による外分泌腺上皮クラスII発現の誘導メカニズム (2008)
  • Author(s): 新垣理恵子
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] 自己免疫病と性差 (2008)
  • Author(s): 石丸直澄等
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-16
• [Presentation] I型糖尿病モデルマウスにおけるカテプシンL阻害剤を用いた治療学的解析 (2008)
  • Author(s): 山田安希子等
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-16
• [Presentation] 自己免疫疾患におけるT細胞の制御機構 (2008)
  • Author(s): 石丸 直澄
  • Organizer: 口腔医科学フロンティア
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2008-02-09
• [Presentation] lRbAp48トランスジェニックマウスを用いたシェーグレン症候群モデルの確立とINFγ産出上皮細胞の解析 (2007)
  • Author(s): lshimaru N. et.al
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-22
• [Presentation] RbAp48トランスジェニックマウスを用いたシェーグレン症候群モデルの確立とIFNγ産出上皮細胞の解析 (2007)
  • Author(s): ISHIMARU Naozumi
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: グランドプリンスホテル新高輪・品川(東京)
  • Year and Date: 2007-11-22
• [Presentation] Crosstalk between RANKL and Fas signaling in dendritic cells controls immune tolerance (2007)
  • Author(s): Izawa T et.al.
  • Organizer: 第37回に本免疫学会総会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-21
• [Presentation] Crosstalk between RANKL and Fas signaling in dendritic cells controls immune tolerance (2007)
  • Author(s): IZAWA Takashi
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: グランドプリンスホテル新高輪・品川(東京)
  • Year and Date: 2007-11-21
• [Presentation] NF-κB1欠損マウスの加齢に伴うT細胞機能の解析 (2007)
  • Author(s): YAMADA Akiko
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: グランドプリンスホテル新高輪・品川(東京)
  • Year and Date: 2007-11-21
• [Presentation] シェーグレン症候群モデルマウスへのTCDD投与による影響 (2007)
  • Author(s): 小橋真之ら
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-20
• [Presentation] シェーグレン症候群モデルマウスへのTCDD投与による影響 (2007)
  • Author(s): KOHASHI Masayuki
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: グランドプリンスホテル新高輪・品川(東京)
  • Year and Date: 2007-11-20
• [Presentation] 自己免疫疾患モデルにおけるNF-κBを介したT細胞活性化機構の解析 (2007)
  • Author(s): OKUNO Miho
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: グランドプリンスホテル新高輪・品川(東京)
  • Year and Date: 2007-11-20
• [Presentation] Development of Autoimmune Exocrinopathy Resembling Sjogren's Syndrome in Rbap48 Transgenic Mice (2007)
  • Author(s): Yoshio Hayashi
  • Organizer: AMERICAN COLLEGE OF RHEUMATOLOGY
  • Place of Presentation: New Boston Convention and Exhibition Center
  • Year and Date: 2007-11-09
• [Presentation] 唾液腺アポトーシスと自己免疫 (2007)
  • Author(s): 石丸 直澄
  • Organizer: 第18回日本口腔病理学会総会・学術大会
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2007-08-11
• [Presentation] Development of autoimmune exocrinopathy resembling Sjogren's syndrome in RbAp48 transgenic mice (2007)
  • Author(s): Hayashi Y
  • Organizer: The 71^<st> Annual Meeting of the American College of Rheumatology
  • Place of Presentation: Boston
• [Presentation] NF-kB2 pathway in T cell activation for autoimmunity (2006)
  • Author(s): Ishimaru N. et.al.
  • Organizer: 第36回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2006-12-13
• [Presentation] Taking advantage of saliva for immune test (2006)
  • Author(s): Ishimaru N.et.al.
  • Organizer: 第36回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2006-12-13
• [Presentation] Immunoregulatory role RANKL-stimulated dendritic cells on on autoimmune arthritis in MRL/lpr mice (2006)
  • Author(s): Izawa T. et.al.
  • Organizer: 第36回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2006-12-13
• [Presentation] The crucial signaling pathway of dendritic cells in metal allergy (2006)
  • Author(s): Wei Hua et.al.
  • Organizer: 第36回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2006-12-13
• [Presentation] Effect of rebamipide administration on Sjogren's syndrome mouse model (2006)
  • Author(s): Kohashi M. et.al.
  • Organizer: 第36回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2006-12-12
• [Presentation] シェーグレン症候群加齢モデルマウスにおける自問質性肺病変の解析 (2006)
  • Author(s): 表原文江ら
  • Organizer: 第95回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2006-05-01
• [Presentation] NFS/sldマウス舌下腺におけるNkx2.3の発現・機能解析 (2006)
  • Author(s): 佐藤亜紀ら
  • Organizer: 第95回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2006-04-30
• [Presentation] NF-kBシグナルを介した自己免疫疾患の発症機序 (2006)
  • Author(s): 石丸直澄ら
  • Organizer: 第95回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2006-04-30
• [Presentation] Cathepsin L-inhibitor prevents proinsulin peptide processing and autoimmune diabetes in nonobase diabetic(NOD) mice (2006)
  • Author(s): Hayashi Y. et.al.
  • Organizer: International Sympojium on Medical and Biological Perspective in Protease and Their Inhibitors
  • Place of Presentation: Awaji, Japan
• [Presentation] RANKL刺激活性化DCを介したMRL/lprマウス関節炎の病態修飾 (2005)
  • Author(s): 井澤俊ら
  • Organizer: 第35回日本免疫学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2005-12-15
• [Presentation] Immunoregulation of NF-kBfor T cell activation in autoimmune disease (2005)
  • Author(s): Ishimaru N. et.al.
  • Organizer: 第35回日本免疫学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2005-12-15
• [Presentation] Mアレルギーにおける樹状細胞及びT細胞の活性化機構 (2005)
  • Author(s): 渡邊恵ら
  • Organizer: 第35回日本免疫学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2005-12-14
• [Presentation] シェーグレン症候群モデルマウスにおけるレバミピドの治療効果の検討 (2005)
  • Author(s): 小橋真之ら
  • Organizer: 第35回日本免疫学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2005-12-13
• [Presentation] シェーグレン症候群加齢モデルマウスにおける聞質性肺病変の解析 (2005)
  • Author(s): 表原文江ら
  • Organizer: 第35回日本免疫学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2005-12-13
• [Presentation] 自己免疫疾患におけるFN-kBを介したT細胞活性化機構の解析 (2005)
  • Author(s): 石丸直澄ら
  • Organizer: 第94回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2005-04-16
• [Presentation] シェーグレン症候群発症の分子基盤 (2005)
  • Author(s): 林良夫
  • Organizer: 第94回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2005-04-14
• [Presentation] RANKL刺激樹状細胞移入によるMRL/lprマウス関節リウマチ病変の解析 (2005)
  • Author(s): 大嶋淳ら
  • Organizer: 第94回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2005-04-14
• [Presentation] シェーグレン症候群発症における自己抗原120kDa-フォドリンの意義 (2005)
  • Author(s): 表原文江ら
  • Organizer: 第94回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2005-04-14
• [Presentation] RbAp48遺伝子導入マウスにみられるシェーグレン症候群類似病変の解析 (2005)
  • Author(s): 新垣理恵子ら
  • Organizer: 第94回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2005-04-14
• [Presentation] Involvement of interstitial pneumonia in Sjogren's syndrome mouse model with aging (2005)
  • Author(s): Hayashi Y. et.al.
  • Organizer: T Cell Immunity & Aging
  • Place of Presentation: Freudenstadt-Lauterbad
• [Presentation] Possible mechanism of autoimmune exocrinopathy resembling Sjogren's syndrome through RbAp48 overexpression (2005)
  • Author(s): Hayashi Y. et.al.
  • Organizer: Gordon Research Conference on Salivary Glads & Exocrine Secretion
  • Place of Presentation: Ventura
• [Presentation] 自己演繹疾患におけるT細胞の制御機構
  • Author(s): 石丸直澄
  • Organizer: 第6回口腔医科学フロンティア
  • Place of Presentation: 東京
• [Book] Autoimmunity-Aging Mouse Model for Autoimmune Disease (2009)
  • Author(s): Hayashi Y., et al
  • Publisher: Handbook on Immunosenescence Springe
• [Book] 口唇生検の診断「シェーグレン症候群の診断と治療」 (2009)
  • Author(s): 林良夫等
  • Publisher: 診断と治療社
• [Book] 口腔内科 (2008)
  • Author(s): 林良夫
  • Publisher: 株式会社飛鳥出版室
• [Book] Annual Review糖尿病・代謝・内分泌2006(エストロゲンと自己免疫疾患) (2006)
  • Author(s): 林 良夫
  • Total Pages: 6
  • Publisher: 中外医学社
• [Patent(Industrial Property Rights)] Composition containingα-fodrin, orα-fodrinfragment protein (2004)
  • Inventor(s): HayashiY, HiromuSugino
  • Industrial Property Rights Holder: Yoshio Hayashi
  • Filing Date: 2004-03-09
• [Patent(Industrial Property Rights)] 疾患の予防・治療剤及びそのスクリーニング方法」 (2003)
  • Inventor(s): 林良夫
  • Industrial Property Rights Holder: 興和株式会社
  • Industrial Property Number: 2003-123959
  • Filing Date: 2003-04-28