Capturing Photo-Induced Protein Dynamics at Picosecond and Angstrom Resolution
| Project/Area Number |
17201044
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | High Energy Accelerator Research Organization (KEK) |
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Principal Investigator |
ADACHI Shinichi High Energy Accelerator Research Organization (KEK), Institute of Materials Structure Science, Associate Professor, 物質構造科学研究所, 助教授 (60260220)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Hideki Osaka City University, Graduate School of Science, Professor, 大学院理学研究科, 教授 (50222211)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2006: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2005: ¥33,930,000 (Direct Cost: ¥26,100,000、Indirect Cost: ¥7,830,000)
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| Keywords | time-resolved / X-ray crystallography / protein / タンバク質 |
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| Research Abstract |
Picosecond time-resolved X-ray research using synchrotron radiation sources is becoming a powerful tool to investigate various dynamics studies of chemical or biological systems and condensed matters. These experiments mostly require relatively sparse bunch-filling mode of the storage ring operation such as single-bunch or hybrid modes, because they require detectors with relatively slow (nanosecond to sub-microsecond) response time. In particular, for time-resolved protein crystallography experiments that utilize two-dimensional area detectors to record a series of time-dependent diffraction patterns, isolation of a single X-ray pulse from X-ray pulse train is a must, because the two-dimensional area detectors such as CCD or imaging plate do not have fast gating capabilities so far. Therefore, in order to isolate a single pulse by using a fast shutter like a mechanical chopper, the sparse bunch-filling modes (single-bunch or hybrid mode) are strongly needed.
Photon Factory Advanced Ring (PF-AR) at the High Energy Accelerator Research Organization (KEK), Tsukuba, Japan is a full-time single-bunch synchrotron radiation source operated for such time-resolved X-ray studies using pulsed X-rays. By utilizing the full advantage of the sparse bunch structure of PF-AR ring, we aimed to develop picosecond time-resolved X-ray structural analysis of protein dynamics at angstrom resolution. Primary scientific targets of the time-resolved X-ray research are photo-induced dynamics in protein crystals which can be triggered reversibly by ultrafast laser pulses. We successfully applied the method to photo-induced ligand-binding dynamics in myoglobin and dimeric hemoglobin crystal. Further efforts are still in progress for initial photo-induced dynamics in bacterial photosynthetic reaction center crystal.
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Report
(3 results)
Research Products
(12 results)
