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Capturing Photo-Induced Protein Dynamics at Picosecond and Angstrom Resolution

Research Project

Project/Area Number 17201044
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionHigh Energy Accelerator Research Organization (KEK)

Principal Investigator

ADACHI Shinichi  High Energy Accelerator Research Organization (KEK), Institute of Materials Structure Science, Associate Professor, 物質構造科学研究所, 助教授 (60260220)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Hideki  Osaka City University, Graduate School of Science, Professor, 大学院理学研究科, 教授 (50222211)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2006: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2005: ¥33,930,000 (Direct Cost: ¥26,100,000、Indirect Cost: ¥7,830,000)
Keywordstime-resolved / X-ray crystallography / protein / タンバク質
Research Abstract

Picosecond time-resolved X-ray research using synchrotron radiation sources is becoming a powerful tool to investigate various dynamics studies of chemical or biological systems and condensed matters. These experiments mostly require relatively sparse bunch-filling mode of the storage ring operation such as single-bunch or hybrid modes, because they require detectors with relatively slow (nanosecond to sub-microsecond) response time. In particular, for time-resolved protein crystallography experiments that utilize two-dimensional area detectors to record a series of time-dependent diffraction patterns, isolation of a single X-ray pulse from X-ray pulse train is a must, because the two-dimensional area detectors such as CCD or imaging plate do not have fast gating capabilities so far. Therefore, in order to isolate a single pulse by using a fast shutter like a mechanical chopper, the sparse bunch-filling modes (single-bunch or hybrid mode) are strongly needed.
Photon Factory Advanced Ring (PF-AR) at the High Energy Accelerator Research Organization (KEK), Tsukuba, Japan is a full-time single-bunch synchrotron radiation source operated for such time-resolved X-ray studies using pulsed X-rays. By utilizing the full advantage of the sparse bunch structure of PF-AR ring, we aimed to develop picosecond time-resolved X-ray structural analysis of protein dynamics at angstrom resolution. Primary scientific targets of the time-resolved X-ray research are photo-induced dynamics in protein crystals which can be triggered reversibly by ultrafast laser pulses. We successfully applied the method to photo-induced ligand-binding dynamics in myoglobin and dimeric hemoglobin crystal. Further efforts are still in progress for initial photo-induced dynamics in bacterial photosynthetic reaction center crystal.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (12 results)

All 2007 2006 2005

All Journal Article (12 results)

  • [Journal Article] ビームライン光学技術シリーズ(11)光の時間構造を使う2007

    • Author(s)
      足立 伸一, 田中 義人
    • Journal Title

      日本放射光学会誌「放射光」 20巻2号

      Pages: 117-123

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Beamline Optics Series (11)-Utilizing Time Structure of Synchrotron Radiation-2007

    • Author(s)
      Shin-ichi Adachi, Yoshihito Tanaka
    • Journal Title

      Synchrotron Radiation (in Japanese) 20

      Pages: 117-117

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Photo-Induced Phase Transition in an Electron-Lattice Correlated Sysytem - Future Role of a Time-Resolved X-ray Measurement for Materials Science -2006

    • Author(s)
      Shin-ya Koshihara
    • Journal Title

      Journal of the Physical Society of Japan 75, 1

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 時間分解X線回折法による光誘起相転移現象の研究2006

    • Author(s)
      足立 伸一, 腰原 伸也
    • Journal Title

      レーザー学会誌「レーザー研究」 38巻8号

      Pages: 538-543

    • NAID

      10018207780

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Photo-Induced Phase Transition in an Electron-Lattice Correlated System - Future Role of a Time-Resolved X-ray Measurement for Materials Science-2006

    • Author(s)
      Shin-ya Koshihara, Shin-ichi Adachi
    • Journal Title

      Journal of the Physical Society of Japan 75

      Pages: 11005-11005

    • NAID

      110003482804

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Time-Resolved X-ray Diffraction as a Tool for Photo-Induced Phase Transition Research2006

    • Author(s)
      Shin-ichi Adachi, Shin-ya Koshihara
    • Journal Title

      Laser Review (in Japanese) 34

      Pages: 538-538

    • NAID

      10018207780

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Photo-Induced Phase Transition in an Electron-Lattice Correlated Sysytem - Future Role of a Time-Resolved X-ray Measurement for Materials Science -2006

    • Author(s)
      Shin-ya Koshihara
    • Journal Title

      Journal of the Physical Society of Japan 75,1

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Current Status of 50-Picosecond Resolved X-ray Diffraction at Photon Factory Advanced Ring (PF-AR)2005

    • Author(s)
      Shin-ichi Adachi
    • Journal Title

      Journal of Physics : Conference Series 21

      Pages: 101-105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] PF-AR NW14, a New Time-Resolved Diffraction/Scattering Beamline2005

    • Author(s)
      Shunsuke Nozawa
    • Journal Title

      Journal of Physics : Conference Series 21

      Pages: 211-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Current Status of 50-Picosecond Resolved X-ray Diffraction at Photon Factory Advanced Ring (PF-AR)2005

    • Author(s)
      Shin-ichi Adachi, et al.
    • Journal Title

      Journal of Physics 21

      Pages: 101-101

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] PF-AR NW14, a New Time-Resolved Diffraction/Scattering Beamline2005

    • Author(s)
      Shunsuke Nozawa, et al.
    • Journal Title

      Journal of Physics 21

      Pages: 211-211

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] PF-AR NF14, a New Time-Resolved Diffraction/Scattering Beamline2005

    • Author(s)
      Shunsuke Nozawa
    • Journal Title

      Journal of Physics : Conference Series 21

      Pages: 211-215

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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