Toxicity test by use of 4-element hybrid strain derived from a Japanese wild rat NIG-III
| Project/Area Number |
17300132
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KITADA Kazuzhiro Hokkaido University, Graduate School of Science, Associate professor (70263093)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,100,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2006: ¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 2005: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | Safety test / rat / outbred strain / wild rats / NIG-III / microstatellite marker / hybrid strain / toxicity test / 交雑群 / 感受性遺伝子 / マイクロサテライトマー |
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| Research Abstract |
Outbred rats are widely used in safety tests for newly developed drugs, however, genetic monitoring and genetic analysis in outbred rodents is not an easy task now Thus, as a tool for toxicity best, we developed a 4-element hybrid derived from a Japanese wild rat NIG-III. The strain NIG-III was originally raised in our facility in 1960s.
Indeed, we successfully developed rat polymorphic microsatellite markers among inbred strains DN, F344, NIG-III and SHR. Furthermore, in order to demonstrate the accuracy and efficacy of genetic monitoring and genetic analysis in a 4-element hybrid strain, we generated a set of hybrid derived from 4 inbred strains DN, F344, NIG-III and SHR and measured their body weights at birth We could easily determined genotypes of the 4-element hybrid individuals, however, QTL analysis on their body weight at birth was unsuccessful.
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Report
(4 results)
Research Products
(10 results)
