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Mechanisms of Retarded Recovery of Muscular Fatigue After Vigorous Contraction

Research Project

Project/Area Number 17300209
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Sports science
Research InstitutionHiroshima University

Principal Investigator

WADA Masanobu  Hiroshima University, Graduate School of Integrated Arts and Sciences, Professor (80220961)

Co-Investigator(Kenkyū-buntansha) MATSUNAGA Satoshi  Osaka City University, Research Center for Urban Health and Sports, Lecturer (70221588)
MISHIMA Takaaki  Hachinohe Junior College, Department of Pre-School Education, Lecturer (00461707)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥6,680,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥3,300,000 (Direct Cost: ¥3,300,000)
Keywordsdegrees of protease activation and / or of oligomeri / sarcoplasmic reticulum / muscular fatigue / calcium ion / muscle contraction / skeletal muscle / Ca^<2+>-ATPase / カルシウム / ATPase / グリコーゲン
Research Abstract

The sarcoplasmic reticulum (SR), a membrane structure in all mu grip evils that closely surrounds each myofibril, plays a critical role in the regulation of intracellular free Ca^<2+> concentration. The present study was undertaken to address the mechanisms underlying retarded recovery of muscular fatigue after vigorous contraction, with focus on changes in the Ca^<2+>-handling ability of the SR Experiments conducted with rat skeletal muscles provided the findings described below.
1. Repetitive muscle contractions hind limb skeletal muscles induced by electric nerve stimulation caused //depressions in SR Ca^<2+>-ATPase activity in the superficial (GS) and deep regions of the gastrocnemius at 1 and 5 min of stimulation, respectively and 2/no changes in the activity in the soleus.
2. Thirty-min recovery after 5-min stimulation resulted in restorations of contraction-induced decreases in SR Ca^<2+>-ATPase activity in GS. However, at this time point, a carbonyl content comprised in the enzym … More e and a fluorescein isothiocyanate (FITC) binding to the enzyme remained increased and decreased, respectively
3. Extraction of glycogen from the SR membrane by treatment with glucoamylase evoked decreases in glycogen phosphorylase on the SR and elevations of FITC binding. However, these alterations were not accompanied by changes in the catalytic activity of SR Ca^<2+>-ATPase.
These findings do not implicate oxidative modifications of protein, structural alterations to the nucleotide-binding domain or depletion of glycogen as a contributor to contraction-induced depressions in the Ca^<2+>-handling ability of the SR It has previously been shown that the rate of restoration of the impaired SR Ca-uptake capacity induced by vigorous muscle contraction varies among different contraction modes, even when immediately after the cessation of contraction, the extent of the depression in SR Ca^<2+>-uptake capacity is similar The distinct restoration rate of SR Ca^<2+>-handling capacity could be explained by the varied degrees of protease activation and/or of oligomerization of SR Ca^<2+-> ATPase. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (20 results)

All 2008 2007 2006 2005 Other

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (9 results) Remarks (2 results)

  • [Journal Article] Time course of changes in in vitro sarcoplasmic reticulum Ca^<2+>-handling and Na^+-K^+-ATPase activity during repetitive contractions2008

    • Author(s)
      Mishima, T.
    • Journal Title

      European Journal of physiology 456

      Pages: 601-609

    • NAID

      120000876468

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Time course of changes in in vitro sarcoplasmic reticulum Ca2+-handling and Na+-K+-ATPase activity during repetitive contractions2008

    • Author(s)
      Mishima, T
    • Journal Title

      European Journal, Physiology 456

      Pages: 601-609

    • NAID

      120000876468

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Time course of changes in in vitro sarcoplasmic reticulum Ca^<2+>- handling and Na^+-K^+-ATPase activity during repetitive contractions2008

    • Author(s)
      Takaaki Mishima
    • Journal Title

      Pflugers Archiv (In press)

    • NAID

      120000876468

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of reduced glycogen on structure and in vitro function of rat sarcoplasmic reticulum2006

    • Author(s)
      Mishima, T.
    • Journal Title

      European Journal of physiology 452

      Pages: 117-123

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 高強度運動における筋疲労の要因:無機リン酸,グリコーゲンおよび活性酸素の影響2006

    • Author(s)
      和田 正信
    • Journal Title

      体育学研究 51

      Pages: 399-408

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of reduced glycogen on structure and in vitr ofunction of rat sarcoplasmic reticulum2006

    • Author(s)
      Mishima, T
    • Journal Title

      European (Journal Physiology) 452

      Pages: 117-123

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Possible factors contributing to muscle fatigue during intense exercise : effects of inorganic phosphate, glycogen and reactive oxygen species2006

    • Author(s)
      Wada, M
    • Journal Title

      Japan, J, Phys. Educ. Hlth. Sport Sci 51

      Pages: 399-408

    • NAID

      110004782803

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 高強度運動における筋疲労の原因 : 無機リン酸、グリコーゲンおよび活性酸素の影響2006

    • Author(s)
      和田 正信
    • Journal Title

      体育学研究 51・4

      Pages: 399-408

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of reduced glycogen on structure and in vitro function of rat sarcoplasmic reticulum2006

    • Author(s)
      Takaaki Mishima
    • Journal Title

      European Journal of Physiology (In press)

    • Related Report
      2005 Annual Research Report
  • [Presentation] 収縮後の回復期における筋小胞体Ca^<2+>-ATPaseの機能変化の要因-ATP結合部位の構造的変化に着目して-2007

    • Author(s)
      三島隆章
    • Organizer
      第62回日本体力医学会
    • Place of Presentation
      秋田市
    • Year and Date
      2007-09-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] 筋疲労後の回復期における筋小胞体Ca^<2+>-ATPaseの機能変化の要-ATP結合部位の構造的変化に着目して-2007

    • Author(s)
      三島 隆章
    • Organizer
      第62回日本体力医学会
    • Place of Presentation
      秋田市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mechanisms underlying changes in function of sarcoplasmic reticulum Ca2+-ATPase during recovery after fatigued muscle contraction2007

    • Author(s)
      Mishima, T
    • Organizer
      62th Annual Congress of Physical and Sports Medicine
    • Place of Presentation
      Akita
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 筋疲労に伴う骨格筋の機能の経時的変化2006

    • Author(s)
      三島 隆章
    • Organizer
      第61回日本体力医学会
    • Place of Presentation
      神戸市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Time course of changes in muscle function during fatigued contraction2006

    • Author(s)
      Mishima, T
    • Organizer
      61t Annual Congress of Physical and Sports Medicine
    • Place of Presentation
      Kobe
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 電気刺激によるCa^<2+>ポンプおよびNa^+-K^+-ポンプの機能の変化2005

    • Author(s)
      三島 隆章
    • Organizer
      第60回日本体力医学会
    • Place of Presentation
      倉敷市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 筋小胞体に付着するグリコーゲンおよびグリコーゲンフォスフォリラーゼがSR Ca^<2+>-ATPaseの機能に及ぼす影響2005

    • Author(s)
      杉山 美奈子
    • Organizer
      第60回日本体力医学会
    • Place of Presentation
      倉敷市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Changes in function Of Ca2+ and Na+-K+ pumps during electrical stimulation-induced muscle contraction2005

    • Author(s)
      Mishima, T
    • Organizer
      60th Annual Congress of Physical and Sports Medicine
    • Place of Presentation
      Kurashiki
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of glycogen and glycogen phosphorylase associated with sarcoplasmic reticulum on Ca2+-ATPase2005

    • Author(s)
      Sugiyama, M
    • Organizer
      60th Annual Congress of Physical and Sports Medicine
    • Place of Presentation
      Kurashiki
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://home.hiroshima-u.ac.jp/wada/index.htm

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/wada/index.htm

    • Related Report
      2007 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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