| Project/Area Number |
17310032
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | University of Toyama |
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Principal Investigator |
KONDO Takashi University of Toyama, Graduate School ofMedicine and Pharmaceutical Sciences, Dept of Radiol Sci, Prof (40143937)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Ryohei University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Dept of Radiol Sci, Assistant, Prof (60334736)
ZHAO Qing-Li University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Dept ofRadioL Sci, Res Associate (90313593)
TABUCHI Yoshikai University of Toyama, T ife Science Research Center, Associate Prof (20322109)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥11,940,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥840,000)
Fiscal Year 2007: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2006: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2005: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Radiation / Hyperthermia / Ultrasound / Microarray / Oxidative stress / アポトーシス / 過酸化水素 / 一酸化窒素 / アルキルラジカル / cDNAマイクロアレイ / 遺伝子発現 |
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| Research Abstract |
For better understanding of the molecular mechanism of hemeoxygenase (HO)-1 as a component of cellular response to non-thermal low intensity pulsed ultrasound(LIPUS, 0.3 W/cm2 for 1 min), and mild hyperthermia (MH, 41℃, 30 min), we examined gene expression patterns and genetic networks in human lymphoma U937 cells using high-density oligonucleotide microarrays and computational gene expression analyses. Six hours after LIPUS treatment, apoptosis (14±3.8%) with no cell lysis was observed. Of 22,283 probe sets analyzed, LIPUS down-regulated 193 genes and up-regulated 201 genes by >1.5-fold. On the other hand, 423 genes were up-regulated and 515 genes were down-regulated in the cells 3 h post-treatment with MH which did not induce apoptosis. The significant genetic networks were identified and the interaction between HO-1 and NRG1 or NF2, and BCL2 or CREB1 was observed in the cells treated with LIPUS and MH, respectively. When the cells were exposed to X-rays, up-regulation of HO-1 was observed in 17937 cells but not in HeLa cells.
The present results indicate that LIPUS and MH affect the expression of many genes and provide novel insight into the biomolecular mechanisms of HO-1. The identification of radiation-responsive genes interacting with HO-1 remains a subject for future studies.
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