• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulating cell behaviors by structurization of intra-/extra-cellular water

Research Project

Project/Area Number 17340125
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics/Chemical physics
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

UCHIDA Tsutomu  Hokkaido Univ., Grad. School of Eng., Asso. Prof., 大学院工学研究科, 助教授 (70356575)

Co-Investigator(Kenkyū-buntansha) GOHARA Kazutoshi  Hokkaido Univ., Grad. School of Eng., Prof., 大学院工学研究科, 教授 (40153746)
NAGAYAMA Masafumi  Hokkaido Univ., Grad. School of Eng., Inst., 大学院工学研究科, 助手 (70374585)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2006: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥11,300,000 (Direct Cost: ¥11,300,000)
Keywordscell / water / structurization / action potential / clathrate hydrate / anesthesia / electron microscope
Research Abstract

The project aimed to reveal the role of water playing on the signal transfer processes between neural cells or cardinal cells by water structurization. For this purpose, we studied the following two themes: 1) observations of the signal transfer in the cellular network and effects of temperature, pressure or composition of culturing circumstances including the clathrate forming components, and 2) development of the observation techniques on the structurized water between cells.
In theme 1), we introduced the measurement system of electronic signals propagating in the cellular network. This system could control the culture conditions (temperature, pressure or compositions of vapor) independently. We observed these conditions effecting on the neural and cardinal cellular networks, especially low temperature and high pressure conditions which might lead the structurization of water, or form clathrate hydrates.
In theme 2), we developed the observation system for structurized water via electron microscope. We first observed the replica samples of freeze-fractured surface on trehalose solution, which is known as one of the cryo-preservation material in the cellular system. Then we observed these samples directly by using the cryo-transfer holder for transmission electron microscopy. Based on these techniques, we introduced the sample preparation system for the water structurization.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (8 results)

All 2007

All Journal Article (8 results)

  • [Journal Article] Morphological investigations of disaccharide molecules for growth inhibition of ice crystals2007

    • Author(s)
      UCHIDA, Tsutomu
    • Journal Title

      Journal of Crystal Growth 299

      Pages: 125-135

    • NAID

      120001117240

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Temporal and spatial variations of lipid droplets during adipocyte division and differentiation2007

    • Author(s)
      NAGAYAMA, Masafumi
    • Journal Title

      Journal of Lipid Research 48

      Pages: 9-18

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Effects of additives and cooling rates on cryo-preservation process of rat cortical cells2007

    • Author(s)
      MOTOMURA, Jutaro
    • Journal Title

      in "Physics and Chemistry of Ice (Ed. by W. F. Kuhs)

      Pages: 409-416

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of additives on formation rates of CO_2 hydrate films2007

    • Author(s)
      UCHIDA, Tsutomu
    • Journal Title

      in "Physics and Chemistry of Ice (Ed. by W. F. Kuhs)

      Pages: 609-617

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Morphological investigations of disaccharide molecules for growth inhibition of ice crystals2007

    • Author(s)
      UCHIDA, Tsutomu, et al.
    • Journal Title

      Journal of Crystal Growth 299

      Pages: 125-135

    • NAID

      120001117240

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Temporal and spatial variations of lipid droplets during adipocyte division and differentiation2007

    • Author(s)
      NAGAYAMA, Masafumi, et al.
    • Journal Title

      Journal of Lipid Research 48

      Pages: 9-18

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of additives and cooling rates on cryo-preservation process of rat cortical cells2007

    • Author(s)
      MOTOMURA, Jutaro, et al.
    • Journal Title

      Physics and Chemistry of Ice (Ed. by W. F. Kuhs) (Royal Society of Chemistry, London)

      Pages: 409-416

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of additives on formation rates of CO_2 hydrate films2007

    • Author(s)
      UCHIDA, Tsutomu, et al.
    • Journal Title

      Physics and Chemistry of Ice (Ed. by W F. Kuhs) (Royal Society of Chemistry, London)

      Pages: 609-617

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi