Regulating cell behaviors by structurization of intra-/extra-cellular water
| Project/Area Number |
17340125
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics/Chemical physics
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
UCHIDA Tsutomu Hokkaido Univ., Grad. School of Eng., Asso. Prof., 大学院工学研究科, 助教授 (70356575)
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| Co-Investigator(Kenkyū-buntansha) |
GOHARA Kazutoshi Hokkaido Univ., Grad. School of Eng., Prof., 大学院工学研究科, 教授 (40153746)
NAGAYAMA Masafumi Hokkaido Univ., Grad. School of Eng., Inst., 大学院工学研究科, 助手 (70374585)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2006: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥11,300,000 (Direct Cost: ¥11,300,000)
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| Keywords | cell / water / structurization / action potential / clathrate hydrate / anesthesia / electron microscope |
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| Research Abstract |
The project aimed to reveal the role of water playing on the signal transfer processes between neural cells or cardinal cells by water structurization. For this purpose, we studied the following two themes: 1) observations of the signal transfer in the cellular network and effects of temperature, pressure or composition of culturing circumstances including the clathrate forming components, and 2) development of the observation techniques on the structurized water between cells.
In theme 1), we introduced the measurement system of electronic signals propagating in the cellular network. This system could control the culture conditions (temperature, pressure or compositions of vapor) independently. We observed these conditions effecting on the neural and cardinal cellular networks, especially low temperature and high pressure conditions which might lead the structurization of water, or form clathrate hydrates.
In theme 2), we developed the observation system for structurized water via electron microscope. We first observed the replica samples of freeze-fractured surface on trehalose solution, which is known as one of the cryo-preservation material in the cellular system. Then we observed these samples directly by using the cryo-transfer holder for transmission electron microscopy. Based on these techniques, we introduced the sample preparation system for the water structurization.
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Report
(3 results)
Research Products
(8 results)
