Fabrication of Microchips Made of Low-RI Fluorinated Polymers and Their Application to μ-TAS
| Project/Area Number |
17350033
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Gunma University |
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Principal Investigator |
TSUNODA Kin-ichi Gunma University, Graduate School of Engineering, Professor (30175468)
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| Co-Investigator(Kenkyū-buntansha) |
ODAKE Tamao University of Tokyo, Graduate School of Engineering, Associate Professor (10301128)
WAKAMATSU Kaori Gunma University, Graduate School of Engineering, Professor (40222426)
HOTTA Hiroki Gunma University, Graduate School of Engineering, Assistant Professor (80397603)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥15,230,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2007: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2006: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 2005: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | Micro-chip / Fluorinated Polymers / Optical Waveguide / Cell Cultivation / Chemical Reactor |
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| Research Abstract |
1) Development of a Teflon AF microchip with a liquid core waveguide absorption detector for μ-TAS
Fabricated is a liquid core waveguide (LCW) analytical micro-chip made of amorphous fluorinated polymer, Teflon AF-1600. The polymer has lower refractive index (1.31) than water, and therefore, the LCW with an aqueous solution as a core could be formed using it as a clad material. The chip was applied to the flow colorimetric analysis of nitrite ion using the Griess-Saltzman reaction. This chip exhibited higher sensitivity than that expected from its path-length (4.4 times), i.e., 7.6 times higher than that with 10 mm cell. The lower limit of detection for nitrite was ca. 20 ngdm^<-3>. Sensitive and convenient colorimetric detection system could be achieved by using this LCW chip.
2) Development of a Teflon AF microchip for cell cultivation
A microchip for cell cultivation was fabricated with glass and Teflon AF, where a channel made in a sheet glass was covered by Teflon AF thin sheet. The Teflon AF sheet may improve the gas-exchange efficiency of the chip due to higher gas-permeability of Teflon-AF. The chip has been applied to the cultivation of human liver cell (Hep G2), but the effectiveness of the Teflon AF sheet for the cultivation has not been proved yet.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Slav Optical Waveguide High-Acidity Sensor Based an Absorbance Change of Protoporphyrin IX2006
Author(s)
Umemura, T, Hotta, H, Abe, T, Takahashi, Y, Takiguchi, H, Uehara, M, Odake, T, Tsunoda,K.
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Journal Title
Analytical Chemistry 78
Pages: 7511-7516
Related Report
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