Dynamics of protein structure alteration at single molecular level

• Project/Area Number: 17360395
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: TAKAGI Masahiro Japan Advanced Institute of Science and Technology, School of Materials Science, Professor (00183434)
• Co-Investigator(Kenkyū-buntansha): HOSAKA Takahiro Japan Advanced Institute of Science and Technology, School of Materials Sciences, Associate Professor (30263619)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥16,190,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥990,000); Fiscal Year 2007: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2006: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 2005: ¥8,300,000 (Direct Cost: ¥8,300,000)
• Keywords: Protein / Structure / Amyloid / Membrane / Interaction / タンパク質 / フォールディング / 構造変化 / 拡張遺伝子工学 / ウシトリプシンインヒビター / ミクログロブリン

Research Abstract

(1) In order to perform analysis of protein structural alteration at single molecular level using FRET method We attempted fluorescence labeling of bavine pancreatic tripsin inhibitor (BFTI) using a new technique of four base codon method. Fluorescent proteins were obtained but not enough yield for studies at molecular level
(2) The effects of negatively charged phosphatidylserine (PS) and neutral phosphatidylcholine (PC) on the structure of wild-type and mutant bovine pancreatic trypsin inhibitor (BPTI) at neutral pH were investigated. The presence of PC did not have any effect on the protein structure whilst PS induced a non-native structure in three mutant BPTI proteins. However, the negatively charged phospholipid did not have any effect on wild-type BPTI. The findings revealed that (i) elimination of some disulphide bonds, results in dramatic change in protein structure, and, (ii) that this biochemical interaction is surface-driven and that electrostatic interactions may play a ver y strong role in influencing the fore-stated changes in protein structure.
(3) Patients on long term hemodyalysis frequently suffer from dialysis-related amyloidosis (DRA). DRA is a pathology associated with a persistent increase of monomeric β2- microglobulin (β2-m) caused by dialysis treatment of renal failure. The mechanism of formation of amyloid fibrils for this protein, as for all the other amyloidogenic proteins, is not clear, and there was great uncertainty about the molecular bases of amyloid deposition when the amyloidogenicity of β2-m was discovered. In this study, we investigated formation of variant β2-m in serum of a patient who receives treatment of hemodialysis. Variant β2-m is derived from restricted cleavage of β2-M. The possible factor involved in formation of variant β2-m was also estimated. The following conclusions are proposed. Formation of variant β2-m, namely restricted cleavage of β2-m, might be caused by proteolytic cleavage by a serum protease(s). However, it is not clear whether the variant β2-m can trigger formation of amyloid. Protein aggregation formation is enhanced in serum from a patient.

Research Products

• [Journal Article] Effect of Phospholipids on conformational structure of bovine pancreatic trypsin inhibitor(BPTI)and its thermolabile mutants (2008)
  • Author(s): Izumikawa, N., et. al.
  • Journal Title: Biopolymers (Epub ahead of print)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Using model membranes for the study of amyloid beta: lipid interactions and neurotoxicity. (2008)
  • Author(s): M. Vestergaard, T. Hamada, M. Takagi
  • Journal Title: Biotechnology and Bioengineering 99 Pages: 753-763
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Chelation of cadmium ions by phytochelatin synthase: role of the cysteine-rich C-terminal. (2008)
  • Author(s): M. Vestergaard, S. Matsumoto, S. Nishikori, K. Shiraki, K. Hirata, M. Takagi
  • Journal Title: Analytical Science. 24 Pages: 277-281
  • NAID: 10021098648
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effect of Phospholipids on conformational structure of bovine pancreatic trypsin inhibitor (BPTI) and its thermolabile mutants [Epub ahead of print] (2008)
  • Author(s): Izumikawa, N., Nishikori, S., Vestergaard, M., Hamada, T., Hagihwara, Y., Yumoto, N., Shiraki, K., Takagi, M
  • Journal Title: Biopolymers
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Chelation of Cadmium Ions by Phytochelatin Synthase : Role of the Cystein-rich C-Terminal, Abstract (2008)
  • Author(s): Vestergaard, M., Matsumoto, S., Nishikori, S., Shiraki, K., Hirata, K., Takagi, M
  • Journal Title: Anal Sci 24 Pages: 277-281
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Using Model Membranes for the Study of Amyloid beta : Lipid Interactions and Neurotoxicity Biotechnology and (2008)
  • Author(s): Vestergaard, M., Hamada, T., Takagi, M
  • Journal Title: Bioengineering 99 Pages: 753-763
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Using model membranes for the study of amyloid beta:lipid interactions and neurotoxicity (2008)
  • Author(s): M.Vestergaard, T.Hamada, M.Takaei
  • Journal Title: Biotechnology and Bioengineering 99 Pages: 753-763
  • Peer Reviewed
• [Journal Article] Chelation of cadmium ions by phytochelatin synthase:role of the cysteine-rich C-ternunal (2008)
  • Author(s): M.Vestergaard, S.Matsumoto, S.Nishikori, K.Shiraki, K.Hirata, M.Takai
  • Journal Title: Analytical Science 24 Pages: 277-281
  • Peer Reviewed
• [Journal Article] Dynamic Processes in endocytic transformation of a raft-exhibitinggiant liposome″ (2007)
  • Author(s): T. Hamada, Y. Miura, K. Ishii, S. Araki, K. Yoshikawa, M. Vestergaard, M. Takagi
  • Journal Title: J. Phys. Chem. B 11 Pages: 10853-10857
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Systematic analysis of aggregates from 38 kinds of non disease-related proteins: identifying the intrinsic propensity of polypeptides to form amyloid fibrils. (2007)
  • Author(s): Y. Aso, K. Shiraki, M. Takagi,
  • Journal Title: Biosci Biotechnol Biochem. 71 Pages: 1313-1321
  • NAID: 10027514990
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Real-time monitoring of dynamic intracellular Ca(2+)movement during early embryogenesis through expression of yellow cameleon. (2007)
  • Author(s): Y. Tsuruwaka, T. Konishi, A. Miyawaki, M. Takagi
  • Journal Title: Zebrafish 4 Pages: 253-260
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 生体モデル膜ラフトドメインの動的構造Vol.45,No.6,436-438(2007) (2007)
  • Author(s): 濱田勉、高木昌宏
  • Journal Title: 化学と生物 45 Pages: 436-438
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Dynamic Processes in Endocytic Transformation of a Raft-Exhibiting Giant Liposome (2007)
  • Author(s): Hamada, T., Miura, Y., Ishii, K., Araki, S., Yoshikawa, K., Vestergaard, M., Takagi, M
  • Journal Title: J. Phys. Chem. B 111 Pages: 10853-10857
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Systematic Analysis of Aggregates from 38 Kinds of Non Disease-Related Proteins : Identifying the Intrinsic Propensity of Polypeptides to Form Amyloid Fibrils (2007)
  • Author(s): Aso, Y., Shiraki, K., Takagi, M
  • Journal Title: Bioscience, Biotechnology and Biochemistry 71 Pages: 1313-1321
  • NAID: 10027514990
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Real-time monitoring of dynamic intracellular Ca(2+) movement during early embryogenesis through expression of yellow cameleon (2007)
  • Author(s): Tsuruwaka, Y., Konishi, T., Miyawaki, A., Takagi, M
  • Journal Title: Zebrafish 4 Pages: 253-260
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dynamic structure of membrane raft domain (2007)
  • Author(s): Hamada, T., Takagi, M
  • Journal Title: Kagaku to Seibutsu 45 Pages: 436-438
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dynamic Processes in endocytic transformation of a raft-exhibitinggiant liposome (2007)
  • Author(s): T.Hamada, Y.Miura, K.Ishii, S.Araki, K.Yoshikawa, M.Vestergaard, M.Takai
  • Journal Title: J.Phys.Chem.B 111 Pages: 10853-10857
  • Peer Reviewed
• [Journal Article] Systematic analysis of aggregates from 38 kinds of non disease-related proteins:identifying the intrinsic propensity of polypeptides to form amyloid fibrils (2007)
  • Author(s): Y.Aso, K.Shiraki, M.Takagi
  • Journal Title: Biosci Biotechnol Biochem 71 Pages: 1313-1321
  • Peer Reviewed
• [Journal Article] Real-time monitoring of dynamic intracellular Ca(2+)movement during early embryogenesis through expression of yellow cameleon (2007)
  • Author(s): Y.Tsuruwaka, T.Konishi, A.Miyawaki, M.Takagi
  • Journal Title: Zebrafish 4 Pages: 253-260
  • Peer Reviewed
• [Journal Article] 生体モデル膜ラフトドメインの動的構造Vo1.45,No.6,436-438(2007) (2007)
  • Author(s): 濱田 勉、高木 昌宏
  • Journal Title: 化学と生物 45 Pages: 436-438
• [Journal Article] 生体モデル膜ラフトドメインの動的構造 (2007)
  • Author(s): 濱田勉, 高木昌宏
  • Journal Title: 化学と生物 45巻6号 Pages: 436-438
  • NAID: 130004808831
• [Journal Article] 小分子添加によるタンパク質の凝集抑制 (2006)
  • Author(s): 工藤基徳、白木賢太郎、高木昌宏
  • Journal Title: バイオサイエンスとインダストリー 64 Pages: 323-327
  • NAID: 10019258046
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enhancing the Tolerance of Zebrafish(Danio rerio)to Heavy Metal Toxicity by the Expression of Plant Phytochelatin Synthase. (2006)
  • Author(s): Konishi, T., et. al.
  • Journal Title: J. Biotechnology 122 Pages: 316-325
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Crystal Structure of Intein Homing Endonuclease II Encoded in DNA Polymerase Gene from Hyperthermophilic Archaeon Thermococcus kodakaraensis Strain KOD1Proteins (2006)
  • Author(s): Matsumura, H., et. al.
  • Journal Title: Structure, Function, and Bioinformatics 63 Pages: 711-715
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Suppression of protein aggregation by small molecules (2006)
  • Author(s): Kudou, M., Shiraki, K., Takagi, M
  • Journal Title: Bioscience and Bioindustry 64 Pages: 323-327
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhancing the Tolerance of Zebrafish (Danio rerio) to Heavy Metal Toxicity by the Expression of Plant Phytochelatin Synthase (2006)
  • Author(s): Konishi, T., Matsumoto, S., Tsuruwaka, Y., Shiraki, K., Hirata, K., Tamaru, Y., Takagi, M
  • Journal Title: J. Biotechnol 122 Pages: 316-325
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Crystal Structure of Intein Homing Endonuclease II Encoded in DNA Polymerase Gene from Hyperthermophilic Archaeon Thermococcus kodakaraensis Strain KOD1 Proteins : Structure (2006)
  • Author(s): Matsumura, H., Takahashi, H., Inoue, T., Yamamoto, T., Hashimoto, H., Nishioka, M., Fujiwara, S., Takagi, M., Imanaka, T., Kai, Y
  • Journal Title: Function and Bioinformatics 63 Pages: 711-715
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 小分子添加によるタンパク質の凝集抑制 (2006)
  • Author(s): 工藤基徳, 白木賢太郎, 高木昌宏
  • Journal Title: バイオサイエンスとインダストリー 64巻6号 Pages: 323-327
  • NAID: 10019258046
• [Journal Article] Crystal structure of intein homing endonuclease II encoded in DNA polymerase gene from hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1. (2006)
  • Author(s): H.Matsumura et al.
  • Journal Title: PROTEINS : Structure, Function, and Bioinformatics 63 Pages: 711-715
• [Journal Article] The First Thermodynamic Characterization of β-1, 3-Xylanase from a Marine Bacterium. (2005)
  • Author(s): Okazaki, F., et. al.
  • Journal Title: Protein Journal 24 Pages: 413-421
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Molecular Cloning and Characterization of Alpha-class Glutathione S-Transferase Genes from the Hepatopancreas of Red sea Bream, Pagrus major. (2005)
  • Author(s): Konishi, T., et. al.
  • Journal Title: Comp Biochem Physiol. C Toxicol. Pharmacol 140 Pages: 309-320
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Unfolding Mechanism of a Hyperthermophilic Protein O^6-methylgu anine-DNA Methyltransferase. (2005)
  • Author(s): Nishikori, S., et. al.
  • Journal Title: Biophysical Chemistry 116 Pages: 97-104
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Combination of Dsb Coexpression and an Addition of Sorbitol Markedly Enhanced Soluble Expression of Single-chain Fv in Escherichia coli. (2005)
  • Author(s): Sandee, D., et. al.
  • Journal Title: Biotechnology and Bioengineering 91 Pages: 418-424
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Comparative Analysis of the Two-step Reaction Catalyzed by Prokaryotic and Eukaryotic Phytochelatin Synthase by an Ion-pair Liquid Chromatography Assay. (2005)
  • Author(s): Tsuji, N., et. al.
  • Journal Title: Planta 222 Pages: 181-191
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Amino Acid Esters Prevent Thermal Inactivation and Aggregation of Lysozyme. (2005)
  • Author(s): Shiraki, et. al.
  • Journal Title: Biotechnology Progress 21 Pages: 640-643
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A New Class of Glutathione S-Transfbrase from the Hepatopancreas of the Red Sea Bream, Pagrus major. (2005)
  • Author(s): Konishi, T.
  • Journal Title: Biochemical Journal 388 Pages: 299-307
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Investigation of Active Form of Catalytic Antibody Light Chain 41S-2-L. (2005)
  • Author(s): Mitsuda, Y., et. al.
  • Journal Title: Immunology Letters 96 Pages: 63-71
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The First Thermodynamic Characterization of □-1,3-Xylanase from a Marine Bacterium (2005)
  • Author(s): Okazaki, F., Shiraki, K., Tamaru, Y., Araki, T., Takagi, M
  • Journal Title: Protein J 24 Pages: 413-421
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular Cloning and Characterization of Alpha-class Glutathione S-Transferase Genes from the Hepatopancreas of Red sea Bream, Pagrus major (2005)
  • Author(s): Konishi, T., Kato, K., Araki, T., Shiraki, K., Takagi, M., Tamaru, Y
  • Journal Title: Comp Biochem Physiol. C Toxicol. Pharmacol 140 Pages: 309-320
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Unfolding Mechanism of a Hyperthermophilic Protein 06-methylguanine-DNA Methyltransferase (2005)
  • Author(s): Nishikori, S., Shiraki, K., Fujiwara, S., Imanaka, T., Takagi, M
  • Journal Title: Biophysical Chemistry 116 Pages: 97-104
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Combination of Dsb Coexpression and an Addition of Sorbitol Markedly Enhanced Soluble Expression of Single-chain Fv in Escherichia coli (2005)
  • Author(s): Sandee, D., Tungpradabku, S., Kurokawa, Y., Fukuil, K., Takagi, M
  • Journal Title: Biotechnology and Bioengineering 91 Pages: 418-424
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparative Analysis of the Two-step Reaction Catalyzed by Prokaryotic and Eukaryotic Phytochelatin Synthase by an Ion-pair Liquid Chromatography Assay (2005)
  • Author(s): Tsuji, N., Nishikori, S., Matsumoto, S., Iwabe, O., Shiraki, K., Miyasaka, H., Takagi, M., Hirata, K., Miyamoto, K
  • Journal Title: Planta 222 Pages: 181-191
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Amino Acid Esters Prevent Thermal Inactivation and Aggregation of Lysozyme (2005)
  • Author(s): Shiraki, K., Kudou, M., Sakamoto, R., Yanagihara, I., Takagi, M
  • Journal Title: Biotechnology Progress 21 Pages: 640-643
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A New Class of Glutathione S-Transferase from the Hepatopancreas of the Red Sea Bream, Pagrus major. (2005)
  • Author(s): Konishi, T., Kato, K., Araki, T., Shiraki, K., Takagi, M., Tamaru, Y
  • Journal Title: Biochem. J 388 Pages: 299-307
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Investigation of Active Form of Catalytic Antibody Light Chain 41S-2-L (2005)
  • Author(s): Mitsuda, Y., Tsuruhata, K., Hifumi, E., Takagi, M., Uda, T
  • Journal Title: Immunology Letters 96 Pages: 63-71
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Stretched-exponential analysis of heat-induced aggregation of apo-concanavalin A (2005)
  • Author(s): Motonori Kudo, Kentaro Shiraki, Masahiro Takagi.
  • Journal Title: Protein Journal 24 Pages: 193-199
• [Presentation] ゼブラフィッシュDAX1遺伝子の機能解析 (2008)
  • Author(s): 矢島 好文, 高木 昌宏
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東海大学海洋学部キャンパス
  • Year and Date: 2008-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Studies on daxl gene from zebrafish (2008)
  • Author(s): Yajima, Y., et. al.
  • Organizer: Meeting of Society of Fishery in Japan
  • Place of Presentation: Tokai University
  • Year and Date: 2008-03-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 非対称2分子膜リポソームの構築 (2008)
  • Author(s): 小松 佑規, 三浦 陽子, 浜田 勉, 高木 昌宏
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学池袋キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] リン脂質の構造に依存した膜小胞動態 (2008)
  • Author(s): 川口 耕平, 石井 健一, 浜田 勉, 高木昌宏
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学池袋キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 細胞膜脂質ラフトドメインの安定性の評価 (2008)
  • Author(s): 山口 健太朗, 鈴木 えり子, 浜田 勉, 高木 昌宏
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学池袋キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Construction of asymmetric membrane bilayer (2008)
  • Author(s): Komatsu, Y., et. al.
  • Organizer: General Meeting of Japan Chemic al Society
  • Place of Presentation: Rikkyo University
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Membrane vesicle movement dependent on the type of phospholipids (2008)
  • Author(s): Kawaguchi, K., et. al.
  • Organizer: General Meeting of Japan Chemical Society
  • Place of Presentation: Rikkyo University
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Evaluation of stability of membrane raft domain (2008)
  • Author(s): Yamaguchi, K., et. al.
  • Organizer: General Meeting of Japan Chemical Society
  • Place of Presentation: Rikkyo University
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 非対称2分子膜リボソームの構築 (2008)
  • Author(s): 小松 佑規, 三浦 陽子, 浜田 勉, 高木 昌宏
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学池袋キャンパス
  • Year and Date: 2008-03-28
• [Presentation] 脂質膜相分離ドメインの内胞ダイナミクス (2008)
  • Author(s): 浜田 勉, 高木昌宏, 三浦陽子
  • Organizer: 日本物理学会 第63回年次大会
  • Place of Presentation: 近畿大学
  • Year and Date: 2008-03-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 脂質膜相分離ドメインの内胞ダイナミクス (2008)
  • Author(s): 浜田 勉, 高木 昌宏, 三浦 陽子
  • Organizer: 日本物理学会第63回年次大会
  • Place of Presentation: 近畿大学
  • Year and Date: 2008-03-23
• [Presentation] Domain-Mediated Endocytic Buddingin a Raft Model Membrane (2008)
  • Author(s): T. Hamada Y. Miura S. Araki K. Yoshikawa M. Takagi
  • Organizer: International Workshop on "Physical Phenomena in Multi-Component Membranes"
  • Place of Presentation: 首都大学 東京
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Domain-mediated endocytic budding in a raft model membrane (2008)
  • Author(s): Hamada, T., et. al.
  • Organizer: International Workshop on physical phenomena
  • Place of Presentation: Tokyo Metropolitan University
  • Year and Date: 2008-03-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Dolnain-Mediated Endocytic Buddin a Raft Model Membrane (2008)
  • Author(s): T.Hamada Y.Miura S.ArakiK.Yoshikawa M.Takagi
  • Organizer: International Workshop on" Physical Phenomena in Multi-Component Membranes
  • Place of Presentation: 首都大学東京
  • Year and Date: 2008-03-17
• [Presentation] Dynamic movement of raft-exhibiting giant liposome (2008)
  • Author(s): M. Takagi, T. Hamada(Invited)
  • Organizer: Swedish-Japanese workshop in Biotechnology
  • Place of Presentation: Alba Nova University Center, Royal Institute of Technology, Stockholm
  • Year and Date: 2008-02-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Dynamic Movement of Raft-exhibiting Giant Liposome Swedish-Japanese Workshop in Biotechnology (2008)
  • Author(s): Takagi, M., Hamada, T
  • Organizer: Alba Nova University Center, Royal Institute of Technology
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2008-02-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Dynamic movement of raft-exhibiting giant liposome (2008)
  • Author(s): M.Takagi, T.Hamada(lnvited)
  • Organizer: Swedish-Japanese workshop in Biotechnology
  • Place of Presentation: Alba Nova University Center,Royal Institute of Technology,Stockholm
  • Year and Date: 2008-02-21
• [Presentation] 脂質膜ラフトドメインのエンドサイトーシス機構 (2008)
  • Author(s): 浜田 勉, 高木昌宏, 三浦陽子
  • Organizer: ソフトマター物理・第2回公開シンポジウム
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-01-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 生体モデル膜ラフトドメインのエンドサイトーシス (2007)
  • Author(s): 浜田 勉, 高木昌宏, 三浦陽子
  • Organizer: 日本生物物理学会第45回年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 生体模倣非対称モデル膜におけるドメイン構造形成 (2007)
  • Author(s): 三浦陽子, 小松佑規, 浜田 勉, 高木昌宏
  • Organizer: 日本生物物理学会第45回年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 光による脂質二分子膜小胞の形態転移 (2007)
  • Author(s): 石井健一, 長崎 健, 浜田 勉, 高木昌宏
  • Organizer: 日本生物物理学会第45回年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Endocytic phenomenon by membrane raft domain (2007)
  • Author(s): Hamada, T., et. al.
  • Organizer: Meeting of Biophysics Society Japan
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2007-12-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Domain structure formation on asymmetric liposome (2007)
  • Author(s): Miura, Y., et. al.
  • Organizer: Meeting of Biophysics Society Japan
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2007-12-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Morphological transformation of membrane structure by optical control (2007)
  • Author(s): Ishii, K., et. al.
  • Organizer: Meeting of Biophysics Society Japan
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2007-12-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 生体モデル膜ラフトドメインのエンドサイトーシス (2007)
  • Author(s): 渡田 勉, 高木 昌宏, 三浦 陽子
  • Organizer: 日本生物物理学会第45回年次大会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2007-12-22
• [Presentation] 生体模倣非対称キデル膜におけるドメイン構造形成 (2007)
  • Author(s): 三浦 陽子, 小松 佑規, 浜田 勉, 高木 昌宏
  • Organizer: 日本生物物理学会第45回年次大会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2007-12-22
• [Presentation] 光にさる脂質二分子膜小胞の形態転移 (2007)
  • Author(s): 石井 健一, 長崎 健, 浜田 勉, 高木 昌宏
  • Organizer: 日本生物物理学会第45回年次大会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2007-12-22
• [Presentation] 光による脂質二分子膜小胞形態のコントロール (2007)
  • Author(s): 石井健一, 畠山剛臣, 長崎 健, 浜田 勉, 高木昌宏
  • Organizer: 第一回 サポーティッドメンブレンワークショップ
  • Place of Presentation: 蒲郡温泉
  • Year and Date: 2007-11-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Morphological control by photosensitive membrane structure (2007)
  • Author(s): Ishii, K., et. al.
  • Organizer: Workshop on supported membrane
  • Place of Presentation: Gamagori Onsen.
  • Year and Date: 2007-11-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 光による脂質二分子膜小胞形態のコントロール (2007)
  • Author(s): 石井 健一, 畠山 剛臣, 長崎 健, 浜田 勉, 高木 昌宏
  • Organizer: 第一回サポーティッドメンブレンワークショップ
  • Place of Presentation: 蒲郡温泉
  • Year and Date: 2007-11-16
• [Presentation] 人工透析アミロイドーシスにおける血中プロテアーゼの影響 (2007)
  • Author(s): 塩野入 純平, 浜田 勉, 毫木昌宏
  • Organizer: 日本化学会近畿支部北陸地区研究発表会
  • Place of Presentation: 金沢大学(角間キャンパス)自然科学本館
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 細胞膜ラフトドメインの動的構造と安定性 (2007)
  • Author(s): 鈴木えり子, 山口健太郎, 浜田 勉, 高木昌宏
  • Organizer: 日本化学会近畿支部北陸地区研究発表会
  • Place of Presentation: 金沢大学(角間キャンパス)自然科学本館
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 可溶化過程における脂質二分子膜の膜揺動 (2007)
  • Author(s): 平林 祐一, 浜田 勉, 高木昌宏
  • Organizer: 日本化学会近畿支部北陸地区研究発表会
  • Place of Presentation: 金沢大学(角間キャンパス)自然科学本館
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 脂質ラフトミクロドメインとAβの相互作用 (2007)
  • Author(s): 谷川晋一, Munde Vestagaard, 浜田 勉, 高木昌宏
  • Organizer: 日本化学会近畿支部北陸地区研究発表会
  • Place of Presentation: 金沢大学(角間キャンパス)自然科学本館
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 生体模倣モデル膜におけるドメイン構造形成 (2007)
  • Author(s): 三浦陽子, 小松佑規, 浜田 勉, 高木昌宏
  • Organizer: 日本化学会近畿支部北陸地区研究発表会
  • Place of Presentation: 金沢大学(角間キャンパス)自然科学本館
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effect of blood proteases on amyloidosis by hemodialysis (2007)
  • Author(s): Shionoiri, J., et. al.
  • Organizer: Meeting of Japan Chemical Society Hokuriku Area
  • Place of Presentation: Kanazawa University
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Dynamic structure and stability of membrane raft domain (2007)
  • Author(s): Suzuki, E., et. al.
  • Organizer: Meeting of Japan Chemical Society Hokuriku Are
  • Place of Presentation: Kanazawa University
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Membrane dynamics of liposomes during solubilization (2007)
  • Author(s): Hirabayashi, Y., et. al.
  • Organizer: Meeting of Japan Chemical Society Hokuriku Area
  • Place of Presentation: Kanazawa University
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Interaction between membrane raft domain and amyloid beta peptide (2007)
  • Author(s): Tanigawa, S., et. al.
  • Organizer: Meeting of Japan Chemical Society Hokuriku Area
  • Place of Presentation: Kanazawa University
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Domain structure formation on biomimetic model membrane (2007)
  • Author(s): Miura, Y., et. al.
  • Organizer: Meeting of Japan Chemical Society Hokuriku Area
  • Place of Presentation: Kanazawa University
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Photo-induced transformation in cell-sized lipid vesicles (2007)
  • Author(s): 石井健一, 長崎 健, 浜田 勉, 高木昌宏
  • Organizer: 1st International Symposium on Ultimate Stability of Nano-structured Polymers and Composites
  • Place of Presentation: 石川ハイテク交流センター国際会議場
  • Year and Date: 2007-10-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Photo-induced transformation in cell-sized lipid vesicles 1st International Symposium on Ultimate Stability of Nano-structured Polymers and Composites Ishi kawa High-tech Center (2007)
  • Author(s): Ishii, K., Nagasaki, K., Hamada, T., Takagi, M
  • Organizer: Ishikawa
  • Place of Presentation: Japan
  • Year and Date: 2007-10-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 脂質二分子膜可熔化プロセスにおける膜揺動ダイナミクス (2007)
  • Author(s): 平林 祐一, 濱田 勉, 高木昌宏
  • Organizer: 日本生物工学会
  • Place of Presentation: 広島大学
  • Year and Date: 2007-09-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 細胞膜脂質ラフトドメインの安定性評価 (2007)
  • Author(s): 山口健太朗、鈴木えり子、濃田 勉, 高木昌宏
  • Organizer: 日本生物工学会
  • Place of Presentation: 広島大学
  • Year and Date: 2007-09-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 脂質二分子膜形態の光制御 (2007)
  • Author(s): 石井健一, 長崎 健, 浜田 勉, 高木昌宏
  • Organizer: 日本生物工学会
  • Place of Presentation: 広島大学
  • Year and Date: 2007-09-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Membrane fluctuation dynamics (2007)
  • Author(s): Hirabayashi, Y., et. al.
  • Organizer: General Meeting of Society of Biotechnology
  • Place of Presentation: Hiroshima University
  • Year and Date: 2007-09-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Studies about stability of membrane raft domain (2007)
  • Author(s): Yamaguchi, K., et. al.
  • Organizer: General Meeting of Society of Biotechnology
  • Place of Presentation: Hiroshima University
  • Year and Date: 2007-09-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Photo-switching of morphology of lipid membrane structure (2007)
  • Author(s): Tshii, K., et. al.
  • Organizer: General Meeting of Society of Biotechnology
  • Place of Presentation: Hiroshima University.
  • Year and Date: 2007-09-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Screening of zebrafish genes involved in morphogenesis using human si RNA library (2007)
  • Author(s): M. Takagi, Tomio Yamauchi, Yoshifumi Yajima(invited)
  • Organizer: The 6th international forum on post-genome technologies
  • Place of Presentation: Bamboo Grove Hotel, Suzhou, China
  • Year and Date: 2007-09-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Screening of Zebrafish Genes Involved in Morphogenesis using Human siRNA Library (2007)
  • Author(s): akagi, M., Yamauchi, T., Yajima, Y
  • Organizer: The 6th international forum on post-genome technologies, Bamboo Grove Hotel
  • Place of Presentation: SuzhouChina
  • Year and Date: 2007-09-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Screening of zebrafish genes involved in morphogenesis using human siRNA library (2007)
  • Author(s): M.Takagi, Tomio Yamauchi, Yoshifumi Yajima(invited)
  • Organizer: The 6th international forum on post-genometechnologies
  • Place of Presentation: Bamboo Grove Hotel,Suzhou,China
  • Year and Date: 2007-09-11
• [Presentation] Screening of zebrafish genes involved in morphogenesis using human si RNAlibrary (2007)
  • Author(s): M. Takagi
  • Organizer: The second international workshop on approaches to single-cell analysis
  • Place of Presentation: Waseda University, Japan
  • Year and Date: 2007-09-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Screening of Zebrafish genes Involved in Morphogenesis using Human siRNA Library (2007)
  • Author(s): Takagi, M
  • Organizer: The second international workshop on approachesto single-cell analysis
  • Place of Presentation: Waseda University, Japan
  • Year and Date: 2007-09-07
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Screening of zebrafish genes involved in morphogenesis using human siRNA library (2007)
  • Author(s): M.Takagi
  • Organizer: The second international workshop on approaches to single-cell analysis
  • Place of Presentation: Waseda University,Japan
  • Year and Date: 2007-09-07
• [Presentation] 脂質2分子膜小胞の光による形態のスイッチング (2007)
  • Author(s): 浜田 勉, 石井健一, 長崎 健, 高木昌宏
  • Organizer: 第10回バイオテクノロジー部会シンポジウム
  • Place of Presentation: 早稲田大学
  • Year and Date: 2007-09-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Photo-switching of dynamic properties of lipid membrane structure. (2007)
  • Author(s): Hamada, T., et. al.
  • Organizer: Biotechnology Symposium
  • Place of Presentation: Waseda University.
  • Year and Date: 2007-09-05
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 巨大リポソーム上ラフトドメインの出芽ダイナミクス (2007)
  • Author(s): 浜田 勉、高木昌宏
  • Organizer: 第1回ソフトマター物理若手勉強会
  • Place of Presentation: 宮島ホテル まこと
  • Year and Date: 2007-08-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Budding dynamics of raft domain on giant liposome (2007)
  • Author(s): Hamada, T., et. al.
  • Organizer: Biophysics Study Forum for Young Researchers
  • Place of Presentation: Miyajima (Hotel Makoto)
  • Year and Date: 2007-08-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 巨大リポソーム上ラフトドメィンの出芽ダイナミクス (2007)
  • Author(s): 浜田 勉、高木 昌宏
  • Organizer: 第1回ソフトマター物理若手勉強会
  • Place of Presentation: 宮島ホテルまこと
  • Year and Date: 2007-08-28
• [Presentation] Endoplasmic reticulum stress responses as criteria to evaluate functional foods and cosmetics (2007)
  • Author(s): M. Takagi, K. Yamaguchi, T. Hamada(Invited)
  • Organizer: The 6th World Congress on Alternatives and Animal Use in the Life Sciences
  • Place of Presentation: Hotel East 21 Tokyo, Japan
  • Year and Date: 2007-08-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Endoplasmic Reticulum Stress Responses as Criteria to Evaluate Functional Foods and Cosmetics (2007)
  • Author(s): Takagi, M., Yamaguchi, K., Hamada, T
  • Organizer: The 6th World Congress on Alternatives and Animal Use in the Life Sciences, Hotel East 21 Tokyo
  • Place of Presentation: Japan
  • Year and Date: 2007-08-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Endoplasmic reticulum stress responses as criteria to evaluate functional foods and cosmetics (2007)
  • Author(s): M.Takagi, K.Yamaguchi, T.Hamada(lnvited)
  • Organizer: The 6th World Congress on Alternatives and Animal Use in the Life Sciences
  • Place of Presentation: Hotel East21 Tokyo,Japan
  • Year and Date: 2007-08-25
• [Presentation] 生体モデル膜ドメインの出芽ダイナミクス (2007)
  • Author(s): 濱田 勉, 高木昌宏, 三浦陽子
  • Organizer: ソフトマター物理・第2回領域研究会
  • Place of Presentation: 米沢市 伝国の杜
  • Year and Date: 2007-06-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Dynamics of membrane budding of raft domain (2007)
  • Author(s): Hamada, T., et. al.
  • Organizer: Soft Matter Sympoium
  • Place of Presentation: Yonezawa
  • Year and Date: 2007-06-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 生体モデル膜ドメインの出芽ダイナミクス (2007)
  • Author(s): 浜田 勉, 高木 昌宏, 三浦 陽子
  • Organizer: ソフトマター物理・第2回領域研究会
  • Place of Presentation: 米沢市伝国の杜
  • Year and Date: 2007-06-22
• [Presentation] リポソーム形態の光スイッチング (2007)
  • Author(s): 濱田 勉, 石井健一, 長崎 健, 高木昌宏
  • Organizer: 日本膜学会第29年会
  • Place of Presentation: 東京理科大学
  • Year and Date: 2007-05-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Photo-switching of liposomal structure (2007)
  • Author(s): Hamada, T., et. al.
  • Organizer: Meeting of Japan Membrane Society
  • Place of Presentation: Tokyo University of Science.
  • Year and Date: 2007-05-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] リボソーム形態の光スイッチング (2007)
  • Author(s): 浜田 勉, 石井 健一, 長崎 健, 高木 昌宏
  • Organizer: 日本膜学会第29年会
  • Place of Presentation: 東京理科大学
  • Year and Date: 2007-05-10
• [Presentation] Endocytic phenomena at raft domain (2007)
  • Author(s): Hamada, T., et. al.
  • Organizer: Meeting of Japan Society of Physics
  • Place of Presentation: Kinki University
  • Year and Date: 2007-03-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Endocytosis of membrane raft domain (2007)
  • Author(s): Hamada, T., et. al.
  • Organizer: Soft Matter Symposium
  • Place of Presentation: Nagoya University
  • Year and Date: 2007-01-07
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 実験科学講座29 バイオテクノロジーの基本技術 第3章遺伝子解析技術 (2006)
  • Author(s): 高木 昌宏
  • Total Pages: 446
  • Publisher: 丸善
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.jaist.ac.jp/ms/labs/takagi/
• [Remarks]
  • URL: http://www.jaist.ac.jp/ms/labs/takagi/
• [Patent(Industrial Property Rights)] 血液回路 (2006)
  • Inventor(s): 高木 昌宏, 林 清秀, 中尾 通治
  • Industrial Property Rights Holder: 北陸先端科学技術大学院大学・日機装
  • Industrial Property Number: 2006-183487
  • Filing Date: 2006-07-03
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 血液回路 (2006)
  • Inventor(s): 高木昌宏他3名
  • Industrial Property Rights Holder: 北陸先端科学技術大学院大学・日機装
  • Industrial Property Number: 2006-183487
  • Filing Date: 2006-07-03