Organelle targeting and folding of membrane proteins
| Project/Area Number |
17370040
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | University of Hyogo |
|---|
Principal Investigator |
SAKAGUCHI Masao University of Hyogo, Graduate School of Life Science, Professor (30205736)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAKAGUCHI Masao University of Hyogo, Graduate School of Life Science, Professor (30205736)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥15,660,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2007: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2006: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2005: ¥5,300,000 (Direct Cost: ¥5,300,000)
|
|---|
| Keywords | Biomembrane / Organelle / Membrane protein / Topology / signal seauence / Biosvntnesis |
|---|
| Research Abstract |
In this research, based on our research background of membrane topogenesis and sorting, we have extensively examined topogenesis process on the endoplasmic reticulum membranes and obtained the following progress. (1) The type I signal-anchor sequence, which translocates the N-terminal portion, can mediate the translocation of N-domain containing DHFR-domain. By using DHFR ligand, MTX, the N-terminal DHFR domain translocation can be regulated. For the translocation of the long N-domain, neither NTP's nor luminal hsp70 homologue, BiP, is required. Ribosome, however, plays a critical function for the N-domain translocation. The driving force for the N-domain at the initial stage is much larger than that of the latter stage. (2) The plant homologue of NHE gene family, NHX1 possesses the same membrane topology as that of animal, indicating the fundamental topology of the NHE gene family members. (3) In topogenesis of membrane proteins on the endoplasmic reticulum, the orientation of the hydrophobic transmembrane segment is influenced by the charge of the flanking amino acid residues. The timing of action of the charges during polypeptide elongation indicated that those function at the ribosome exit sites and the commitment of the hydrophobic segment to a particular orientation is influenced by far downstream parts of the polypeptide chain. (4) We developed novel regulatable experiment system for N-domain translocation. Two translocating hydrophilic segment in a single membrane protein can span the membrane during multispanning topogenesis flanking the translocon. Furthermore, even after six successive hydrophobic segments entered the translocon, N-domain translocation could be induced to restart from an arrested state. The remarkably flexible nature of the translocon was indicated.
|
Report
(4 results)
Research Products
(62 results)
-
-
-
-
-
[Journal Article] Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K^+-channel voltage-sensor domain2007
Author(s)
Zhang, L., Sato, Y., Hessa, T., von Heijne, G., Lee, J.-K., Kodama, I., Sakaguchi, M., and Uozumi, N.
-
Journal Title
Proc.Natl.Acad.Sci.USA 104
Pages: 8263-8268
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
[Journal Article] Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K^+-channel voltage-sensor domain2007
Author(s)
Zhang, L., Sato, Y., Hessa, T., von Heijne, G., Lee, J.-K., Kodama, I., Sakaguchi, M., Uozumi, N
-
Journal Title
Proc. Natl. Acad. Sci. USA 104
Pages: 8263-8268
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
[Journal Article] Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K+-channel voltage-sensor domain2007
Author(s)
Zhang, L., Sato, Y., Hessa, T., von Heijne, G., Lee, J.-K, Kodama, I., Sakaguchi, M., and Uozumi, N.
-
Journal Title
Proc.Natl.Acad.Sci.USA 104
Pages: 8263-8268
Related Report
Peer Reviewed
-
-
[Journal Article] Vestibular hair bundles control pH with(Na^+,K^+)/H^+ exchangers NHE6 and NHE92006
Author(s)
Hill, J.K., Brett, C.L., Chyou, A., Kallay, L.M., Sakaguchi, M., Rao, R., and Gillespie, P.G.
-
Journal Title
J.Neurosci 26
Pages: 9944-9955
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
[Journal Article] Vestibular hair bundles control pH with(Na^+, K^+)/H^+ exchangers NHE6 and NHE92006
Author(s)
Hill, J.K., Brett, C.L., Chyou, A., Kallay, L.M., Sakaguchi, M., Rao, R. Gillespie, P.G
-
Journal Title
J. Neurosci 26
Pages: 9944-9955
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
[Journal Article] Vestibular hair bundles control PH with (Na^+, K^+)/H^+ exchangers NHE6 and NHE92006
Author(s)
Hill, J.K., Brett, C.L., Chyou, A., Kallay, L.M., Sakaguchi, M., Rao, R., Gillespie, P.G.
-
Journal Title
J. Neurosci. 26
Pages: 9944-9955
Related Report
-
-
-
-
-
[Journal Article] Role of Pex19p in the Targeting of PMP70 to Peroxisome2005
Author(s)
Kashiwayama, Y., Asahina, K., Shibata, H., Morita, M., Muntau, A.C., Roscher, A.A., Wanders, R.J.A, Shimozawa, N., Sakaguchi, M., Kato, K., and Imanaka, T.
-
Journal Title
Biochim.Biophys.Acta 1746
Pages: 116-128
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
[Journal Article] Role of Pex19p in the Targeting of PMP70 to Peroxisome2005
Author(s)
Kashiwayama, Y., Asahina, K., Shibata, H., Morita, M., Muntau, A.C., Roscher, A.A., Wanders, R.J.A, Shimozawa, N., Sakaguchi, M., Kato, K., Imanaka, T
-
Journal Title
Biochim. Biophys. Acta 1746
Pages: 116-128
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 小胞体膜タンパク質膜透過の駆動力2005
Author(s)
森本 富美子、木田 祐一郎、阪口 雅郎
Organizer
第78回日本生化学会大会
Place of Presentation
神戸
Year and Date
2005-10-19
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
-
-
-
