Construction of linkage maps in Radish and QTL analysis of root traits
| Project/Area Number |
17380006
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
HIRAI Masashi Kyoto Prefectural University, Kyoto Prefectural University, Agriculture, Professor (50343405)
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| Co-Investigator(Kenkyū-buntansha) |
KUBO Nakao Kyoto Prefectural University, Kyoto Prefectural University, Agriculture, Associate Professor (60347440)
FUJIMURA Tatsuhito Tsukuba University, Life and Environm. Sci., Professor (70292513)
OHTA Masaru Thukuba University, Life and Enviromn. Sci., Lecturer (00361311)
TSURO Masato Kyoto Prefectural University, Meijo University, Agriculture, Lecturer (40410774)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥17,080,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2007: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2006: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2005: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | Raphanus sativus / Simple sequence repeat / linkage map / Root shape / flowering / genetic diversity / clubroot resistance / phylogeny / 系統関係 / 野生集団 / 根こぶ病抵抗性 / QTL / 抽台 / マイクロサテライト / 根部形質 / 形態形成 |
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| Research Abstract |
A linkage map was constructed by the group of Kyoto Prefectural University and Meijo University using a segregating population derived from a crossing of a Chinese radish having spherical root and a Japanese radish having cylindrical root. In this map, we identified QTLs for root shape and root anthocyanin pigmentation. We also mapped clubroot resistance loci by a QTL analysis. The results indicate that the resistance is controlled by a major gene or closely linked major genes. Vernalization-induced flowering of radish was studied another segregating population derived from a crossing of rat-tailed radish from South-east Asia and a Japanese radish. The former parent does not required low temperature for flowering unlike Japanese radishes. The flowering is controlled by a single major gene. Linkage markers for the flowering gene were obtained by a bulked segregant analysis. The linkage makers showed base homologies with genomic sequences in lower arm of chromosome 1 of Arabidopsis.
Another linkage map was constructed by the group of Tsukuba University based on a crossing of Shogoin radish and a Taiwanese radish. QTLs for root shape are obtained. Anumber of genetic markers for radish were developed by this group.
Phylogenic relationship and genetic diversity were studied using radish germplasm collected United States and Eurasian continent. They are grouped by the original area of the lines. Any bottle neck was observed as far as examined.
Genetic relationship and the diversity of Shogoin radishes and other Japanese radish were studied. Shogoin radishes showed markedly low genetic diversity calculated by allele frequency of SSR.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Mapping of QTLs controlling root shape and red pigmentation in radish, Raphanus sativus L.2008
Author(s)
Tsuro, M., Suwabe, K., Kubo, N., Matsumoto, S., Hirai, M.
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Journal Title
Breed.Sci. 58
Pages: 55-61
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Brassica rapa2007
Author(s)
Hirai, M., S.Matsumoto
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Journal Title
Genome mapping and molecular breeding in plants(Springer Verlag) Vol.5
Pages: 129-134
Description
「研究成果報告書概要(欧文)」より
Related Report
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