Molecular mechanism of gene targeting in insect cells and its applications

• Project/Area Number: 17380037
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kyushu University
• Principal Investigator: KUSAKABE Takahiro Kyushu University, Graduate School of Agriculture, Associate Professor (30253595)
• Co-Investigator(Kenkyū-buntansha): KAWAGUCHI Yutaka Kyushu University, Graduate School of Agriculture, Professor (80038306) ; LEE Jaeman Kyushu University, Graduate School of Agriculture, Assistant Professor (50404083)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥16,680,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥1,380,000); Fiscal Year 2007: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2006: ¥4,900,000 (Direct Cost: ¥4,900,000); Fiscal Year 2005: ¥5,800,000 (Direct Cost: ¥5,800,000)
• Keywords: SILKWORM / TARGETING / HOMOLOGOUS RECOMBINATION / INSECT / GENE DISRUPTION / CHROMATIN / 相同組換え

Research Abstract

Gene targeting is a method to manipulate a specific genomic loci by using homologous recombination that is one of an intrinsic DNA repair system. Since only the target gene can be disrupted precisely, this is a faithful method as compared with RNAi.
In this project, to improve the gene targeting efficiency, we constructed in vivo homologous recombination (HR) assay system and screened the genes related to HR in insect cells. As a result, we isolated silkworm AGO2 gene as a suppressor of HR at an extra-chromosomal DSB site. Furthermore, our results suggested that the Ago2 protein, at least in part, plays an important role to discriminate whether DSB is on the chromosome or not, through chromatin.
Consequently, we isolated and characterized chromatin regulators from silkworm cells to utilize the improvement of gene targeting efficiency. In silkworm chromatin, histone H3K9 was possible to be mono-, di-, or tri-methylated by. Su(Var)3-9. Furthermore, methylated H3K9 was recognized by HP1a and HP1b. Compared with other organisms, recruitment of the HP1a to the 5'-flanking region, however, is less effective to induce the transcription repression via hetrochromatin formation.

Research Products

• [Journal Article] Construction of gene expression systemsin in sect cell lines using proomoters from the silkworm, Bombyx mori (2008)
  • Author(s): Lee, et. al.
  • Journal Title: J. Bioteclmol. 133 Pages: 9-17
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori (2008)
  • Author(s): Lee, J., Takahashi, M., Mon, H., Nakajima, Y., Koga, K., Kawaguchi, Y., Kusakabe, T.
  • Journal Title: J.Biotechnol 133 Pages: 9-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Construction of gene expression systems in insect cell lines using promoters from the silkworm,Bombyx mori (2008)
  • Author(s): Lee, et. al.,
  • Journal Title: J.Biotechnol. 133 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Heterotrimeric Complex of Replication Protein A Protein, from the. Silkworm, Bombyx mori (2007)
  • Author(s): Sugahara, et. al.
  • Journal Title: J. Insect Biotech. Seric. 76 Pages: 129-135
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Molecular Cloning of Silkworm Cdc37 and its Interaction with Hsp90 Chaperon (2007)
  • Author(s): Yamashita, et. al.
  • Journal Title: J. Insect Biotech. Seric. 76 Pages: 137-143
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Screening of high-permissive silkworm strains for efficient recombinant protein production in Autographa californica nuclear polyhedrosis virus (AcNPV) (2007)
  • Author(s): Lee, et. al.
  • Journal Title: J. Insect Biotech. Seric. 76 Pages: 101-106
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The gacA gene of Pseudomonas aeruginosa PAO1 is not required for full virulence in Bombvx mori (2007)
  • Author(s): Chieda, et. al.
  • Journal Title: J. Insect Biotech. Seric. 76 Pages: 86-106
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effect of inactivation of the superoxide dismutase genes on the virulence of Pseudomonas aeruginosa PAO1 to silkworm, Bombvx mori (2007)
  • Author(s): Iiyama, et. al.
  • Journal Title: Appl. Environ. Microbiol 73 Pages: 1569-1575
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characteristic of Se, the white-sided egg mutation in Bombyx mori (2007)
  • Author(s): Kawaguchi, et. al.
  • Journal Title: J. Insect Biotech. Seric 76 Pages: 71-78
  • NAID: 130004935561
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis (2007)
  • Author(s): Zhang, et. al.
  • Journal Title: J. Proteome res. 6 Pages: 2295-2303
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characteristic of Se the white-sided egg mutation in Bombyx mori (2007)
  • Author(s): Kawaguchi, Y., Yoshida, Y., Kusakabe, T., Lee, J., Koga, K.
  • Journal Title: J.Insect Biotech. Seric 76 Pages: 71-78
  • NAID: 130004935561
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The gacA gene of Pseudomonas aerugmosa PA01 is not required for full virulence in Bombyx mori. J.Insect Biotech.Seric.76, 86-96 (2007)
  • Author(s): Chicda, Y., Iiyama, K., Lee, J., Kusakabe, T., Yasunaga-Aoki, C., Lee, J., Kusakabe, T., Shimizu, S.
  • Journal Title: J.Insect Biotech. Serie 76 Pages: 86-96
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of inactivation of the superoxide dismutase genes on the virulence of Pseudomonas aeruginosa PAO1 to silkwonn, Bombyx mori (2007)
  • Author(s): Iiyama, K., Chieda, Y., Lee, J., Kusakabe, T., Yasunaga-Aoki, C., Lee, J., Kusakabe, T., Shimizu, S.
  • Journal Title: Appl.Environ. Microbiol 73 Pages: 1569-1575
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Screening of high-permissive silkworm strains for efficient recombinant protein production in Autographa californica nuclear polyhedrosis virus (AcNPV) (2007)
  • Author(s): Lee, J., Mon, H., Takahashi, M., Kawakami, N., Aoki, Y., Yoshida, Y., Banno, Y., Koga, K., Kawaguchi, Y., Kusakabe, T.
  • Journal Title: J.Insect Biotech. Seric 76 Pages: 101-106
  • NAID: 130004935558
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis (2007)
  • Author(s): Zhang, P., Aso, Y., Jikuya, H., Kusakabe, T., Lee, J., Kawaguchi, Y., Yamamoto, K., Banno, Y., Fujii, H.
  • Journal Title: J.Proteome res 6 Pages: 2295-2303
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular Cloning of Silkworm Cdc37 and its Interaction with Hsp90 Chaperon (2007)
  • Author(s): Yamashita, J., Miyagawa, Y., Sugahara, R., Mon, H., Mitsunobu, H., Lee, J., Kawaguchi, Y., Kusakabe, T.
  • Journal Title: J.Insect Biotech.Seric 76 Pages: 137-143
  • NAID: 10019767230
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Heterotrimeric Complex of Replication Protein A, a Single-Stranded DNABinding Protein, from the Silkworm, Bombyx mori (2007)
  • Author(s): Sugahara, R., Mon, H., Yamashita, J., Mitsunobu, H., Lee, J., Kawaguchi, Y., Koga, K., Kusakabe, T.
  • Journal Title: J.Insect Biotech.Seric 76 Pages: 129-135
  • NAID: 10019767207
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Heterotrimeric Complex of Replication Protein A,a Single-Stranded DNABinding Protein,from the Silkworm,Bombyx mori (2007)
  • Author(s): Sugahara, et. al.
  • Journal Title: J.Insect Biotech.Seric. 76 Pages: 129-135
  • Peer Reviewed
• [Journal Article] Molecular Cloning of Silkworm Cdc37 and its Interaction with Hsp90 Chaperon (2007)
  • Author(s): Yamashita, et. al.,
  • Journal Title: J.Insect Biotech.Seric. 76 Pages: 137-143
  • Peer Reviewed
• [Journal Article] Screening of high-permissive silkworm strains for efficient recombinant protein production in Autographa californica nuclear polyhedrosis virus(AcNPV) (2007)
  • Author(s): Lee, et. al.
  • Journal Title: J.Insect Biotech.Seric. 76 Pages: 101-106
  • Peer Reviewed
• [Journal Article] The gacA gene of Pseudomonas aeruginosa PAO1 is not required for full virulence in Bombyx mori (2007)
  • Author(s): Chieda, et. al.,
  • Journal Title: J.Insect Biotech.Seric. 76 Pages: 86-106
  • Peer Reviewed
• [Journal Article] Effect of inactivation of the superoxide dismutase genes on the virulence of Pseudomonas aeruginosa PAO1 to silkworm,Bombyx mori (2007)
  • Author(s): Iiyama, et. al.,
  • Journal Title: Appl.Environ.Microbiol 73 Pages: 1569-1575
  • Peer Reviewed
• [Journal Article] Characteristic of Se,the white-sided egg matation in Bombyx mori (2007)
  • Author(s): Kawaguchi, et. al.,
  • Journal Title: J.Insect Biotech.Seric. 76 Pages: 71-78
  • Peer Reviewed
• [Journal Article] Proteomic Profiling of the silkworm muscle proteins during larval-pupal metamorphosis (2007)
  • Author(s): Zhang, et. al.,
  • Journal Title: J.Proteome res. 6 Pages: 2295-2303
  • Peer Reviewed
• [Journal Article] Site-specific gene integeration in cultured silkworm cells mediated by fC31 integrase (2006)
  • Author(s): Nakayama G., et. al.
  • Journal Title: Mol.Genet.Genomics 275 Pages: 1-8
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cell Cycle Arrest induced by Radiation in Culutured Silkworm Cells (2006)
  • Author(s): Takahashi M., et. al.
  • Journal Title: J.Insect Biotech.Seric. 75 Pages: 23-30
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Micropylar structure of chorion of the female sterile mutation,bd, in,Bombyx mori (2006)
  • Author(s): Kawaguchi, Y., et. al.
  • Journal Title: J.Insect Biotech.Seric. 75 Pages: 9-14
  • NAID: 10017316105
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Role of the Silkworm Argonaute2 Homologue Gene in Double-Strand Break Repair of Extrachromosomal DNA (2006)
  • Author(s): Tsukioka H., et. al.
  • Journal Title: Nucleic Acids Res. 34 Pages: 1092-1101
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Radiation resistance and its inheritance in silkworm,Bombyx mori (2006)
  • Author(s): Takahashi M., et. al.
  • Journal Title: J.Fac.agr., Kyushu Univ. 51 Pages: 261-264
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Construction of gateway-based destination vectors for detecting subcellular localizatio n of proteins in the silkworm,Bonbyx mori (2006)
  • Author(s): Mitsunobu, H., et. al.
  • Journal Title: J.Insect Biotech.Seric. 75 Pages: 141-146
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Site-specific gene integration in culturedsilkworm cells mediated by fC31 integrase (2006)
  • Author(s): Nakayama, G., Kawaguchi, Y., Koga, K., Kusakabe, T.
  • Journal Title: Mol. Genet.Genomics 275 Pages: 1-8
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cell Cycle Arrest induced by Radiation in Cultured Silkworm Cells (2006)
  • Author(s): Takahashi, M., Lee, J., Mon, H., Kawaguchi, Y., Koga, K., Kusakabe, T.
  • Journal Title: J.Insect Biotech.Seric 75 Pages: 23-30
  • NAID: 10017316146
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Micropylar structure of cborjon of the female sterile mutation, bd, in, Bombyx mori (2006)
  • Author(s): Kawaguchi, Y., Kusakabe, T., Lee, J., Nakajima, Y., Koga, K.
  • Journal Title: J.Insect Biotech.Seric 75 Pages: 9-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of the Silkworm Argonaute2 Homologue Gene in Double-Strand Break Repair of Extrachromosomal DNA (2006)
  • Author(s): Tsukioka, H., Takahashi, M., Mon, H., Okano, K., Mita, K., Shimada, T., Lee, J., Kawaguchi, Y., Koga, K., Kusakabe, T.
  • Journal Title: Nucleic Acids Res 34 Pages: 1092-1101
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Radiation resistance and its inheritance in the silkworm, Bombyx mori (2006)
  • Author(s): Takahashi, M., Lee, J., Mon, H., Yoshida, H., Kawaguchi, Y., Maekawa, H., Koga, K., Kusakabe, T.
  • Journal Title: J.Fac.Agr., (Kyushu Univ) 51 Pages: 261-264
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Construction of gateway-based destination vectors for detecting subcellular localization of proteins in the silkworm, Bombyx mori (2006)
  • Author(s): Mitsunobu, H., Sakashita, K., Mon, H., Lee, J., Kawaguchi, Y., Koga, K., Kusakabe, T.
  • Journal Title: J.Insect Biotech. Seric 75 Pages: 141-146
  • NAID: 10020516432
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Site-specific gene integration in cultured silkworm cells mediated by ΦC31 integrase. (2006)
  • Author(s): Nakayama G.et al.
  • Journal Title: Mol.Genet.Genomics 275 Pages: 1-8
• [Journal Article] Cell Cycle Arrest induced by Radiation in Cultured Silkworm Cells. (2006)
  • Author(s): Takahashi M.et al.
  • Journal Title: J.Insect Biotech.Seric. 75 Pages: 23-30
• [Journal Article] Micropylar structure of chorion of the female sterile mutation,bd,in,Bombyx mori. (2006)
  • Author(s): Kawaguchi Y.et al.
  • Journal Title: J.Insect Biotech.Seric. 75 Pages: 9-14
  • NAID: 10017316105
• [Journal Article] Role of the Silkworm Argonaute2 Homologue Gene in Double-Strand Break Repair of Extrachromosomal DNA. (2006)
  • Author(s): Tsukioka H.et al.
  • Journal Title: Nucleic Acids Res. 34 Pages: 1092-1101
• [Journal Article] Radiation resistance and its inheritance in the silkworm,Bombyx mori. (2006)
  • Author(s): Takahashi M.et al.
  • Journal Title: J.Fac.Agr.,Kyushu Univ.51 51 Pages: 261-264
• [Journal Article] Construction of gateway-based destination vectors for detecting subcellular localization of proteins in the silkworm,Bombyx mori. (2006)
  • Author(s): Mitsunobu H.et al.
  • Journal Title: J.Insect Biotech.Seric. 75 Pages: 141-146
• [Journal Article] Efficient nonviral gene transfer mediated by polyethylenimine in an insect cell line (2005)
  • Author(s): Maeda, T., et. al.
  • Journal Title: J.Insect Biotech. Seric. 74 Pages: 21-26
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Inheritance of gon, a body color mutation of newly hatched larvae in Bombyx mori (2005)
  • Author(s): Kawaguchi, Y., et. al.
  • Journal Title: J.Insect Biotech. Seric. 74 Pages: 35-37
  • NAID: 10015434007
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell lineBmN4 (2005)
  • Author(s): Ohsaki, A., et. al.
  • Journal Title: Mol. Biol. Reports 32 Pages: 25-34
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cloning and Characterization of a Ribonuclease L Inhibitor from the Silkworm,Bombyx mori (2005)
  • Author(s): Maeda, T., et. al.
  • Journal Title: DNA Sequence 16 Pages: 21-27
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differential expression of a Bombyx mori AHA1 homologue during spermatogenesis (2005)
  • Author(s): Miyagawa, Y., et. al.
  • Journal Title: Insect Mol. Biol. 14 Pages: 245-253
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Evidence of defect in fibroin secretion in the posterior silk gland cells of the tic mutant of Bonbyx mori (2005)
  • Author(s): Sakaguchi, B., et. al.
  • Journal Title: J.Insect Biotech. Seric. 74 Pages: 75-78
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Efficient gene transfer into silkworm larval tissues by a combination of sonoporationand lipofection (2005)
  • Author(s): Lee, J., et. al.
  • Journal Title: Cell Biol. Int. 29 Pages: 976-979
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Efficient nonviral gene transfer mediated by polyethylenimine in an insect cell line (2005)
  • Author(s): Madea, T., Kusakabe, T., Lee, J., Miyagawa, Y., Kawaguchi, Y., Koga, k.
  • Journal Title: J. Insect Biotech.Seric 74 Pages: 21-26
  • NAID: 10015433963
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inheritance of gon, a body color mutation of newly hatched larvae in Bombyx mori (2005)
  • Author(s): Kawaguchi, Y., Doira, H., Ohta, K., Kusakabe, T., Koga K.
  • Journal Title: J.Insect Biotech. Seric 74 Pages: 35-37
  • NAID: 10015434007
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori (2005)
  • Author(s): Chieda, Y., Iiyama, K., Yasunaga-Aoki, C., Lee, J., Kusakabe, T., Shimizu, S.
  • Journal Title: FEMS Microbiol. Lett 244 Pages: 181-186
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4 (2005)
  • Author(s): Ohsaki, A., Iiyama, K., Miyagawa, Y., Kawaguchi, Y., Koga, K., Kusakabe, T.
  • Journal Title: Mol.Biol.Reports 32 Pages: 25-34
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cloning and Characterization of a Ribonuclease L Inhibitor from the Silkworm, Bombyx Mori (2005)
  • Author(s): Maeda, T., Kusakabe, T., Lee, J., Miyagawa, Y., Kawaguchim, Y., Koga, K.
  • Journal Title: DNA Sequence 16 Pages: 21-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential expression of a Bombyx mori AHA1 homologue during spermatogenesis. Insect Mol.Biol.14, 245-253 (2005)
  • Author(s): Miyagawa, Y., Lee, J., Maeda, T., Koga, K., Kawaguchi, Y., Kusakabe, T.
  • Journal Title: Insect Mol.Biol 14 Pages: 245-253
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Efficient gene transfcr into silkworm larval tissues by a combination of sonoporation and lipofection (2005)
  • Author(s): Lee, J., Takahashi, M., Mon, H., Koga, K., Kawaguchi, Y., Kusakabe, T.
  • Journal Title: Cell Biol.Int 29 Pages: 976-979
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evidence of defect in fibroin secretion in the posterior silk gland cells of the flc mutant of Bombyx mori.J.Insect Biotech.Seric.74,75-78 (2005)
  • Author(s): Sakaguchi, B., Kawaguchi, Y., Koga, K., Kusakabe, T.
  • Journal Title: J.Insect Biotech. Seric 74 Pages: 75-78
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Efficient nonviral gene transfer mediated by polyethylenimine in an insect cell line. (2005)
  • Author(s): Maeda, T. et al.
  • Journal Title: J.Insect Biotech.Seric. 74 Pages: 21-26
• [Journal Article] Inheritance of gon, a body color mutation of newly hatched larvae in Bombyx mori. (2005)
  • Author(s): Kawaguchi, Y. et al.
  • Journal Title: J.Insect Biotech.Seric. 74 Pages: 35-37
  • NAID: 10015434007
• [Journal Article] Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4. (2005)
  • Author(s): Ohsaki, A. et al.
  • Journal Title: Mol.Biol.Reports 32 Pages: 25-34
• [Journal Article] Cloning and Characterization of a Ribonuclease L Inhibitor from the Silkworm, Bombyx mori. (2005)
  • Author(s): Maeda, T. et al.
  • Journal Title: DNA Sequence 16 Pages: 21-27
• [Journal Article] Differential expression of a Bombyx mori AHA1 homologue during spermatogenesis. (2005)
  • Author(s): Miyagawa, Y. et al.
  • Journal Title: Insect Mol.Biol. 14 Pages: 245-253
• [Journal Article] Evidence of defect in fibroin secretion in the posterior silk gland cells of the flc mutant of Bombyx mori. (2005)
  • Author(s): Sakaguchi, B. et al.
  • Journal Title: J.Insect Biotech.Seric. 74 Pages: 75-78
• [Journal Article] Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection. (2005)
  • Author(s): Lee, J.et al.
  • Journal Title: Cell Biol.Int. 29 Pages: 976-979
• [Presentation] カイコにおける融合型GAL4転写因子の利用 (2007)
  • Author(s): 小林, ら
  • Organizer: 日本分子生物学会本会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カイコにおけるHPlaとメチル化ピストンH3の相互作用 (2007)
  • Author(s): 光延, ら
  • Organizer: 日本分子生物学会本会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カイコのヘテロクロマチン形成におけるζストンメチルトランスフェラーゼSu(var)3-9の機能解析 (2007)
  • Author(s): 竹野, ら
  • Organizer: 日本分子生物学会本会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カイコにおけるMnd1とHop2の機能解析 (2007)
  • Author(s): 戸畑, ら
  • Organizer: 日本分子生物学会本会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カイコにおけるFA複合体の解析 (2007)
  • Author(s): 管原, ら
  • Organizer: 日本分子生物学会本会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カイコBrca2の機能解析 (2007)
  • Author(s): 門, ら
  • Organizer: 日本分子生物学会本会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カイコのヘテロクロマチン形成におけるヒストンメチルトランスフェラーゼSu(var)3-9の機能解析 (2007)
  • Author(s): 竹野, ら
  • Organizer: 日本分子生物学会本会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] Molecular cloning and characterization of HPla, HPlb in the sillcworm, Bombyx mori (2007)
  • Author(s): Mitsunobu, et. al.
  • Organizer: International congress of insect biotechnology & industry
  • Place of Presentation: Daegu; Korea
  • Year and Date: 2007-08-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Moleculer Cloning of Piwi and Aubergine homolog genes from the silkworm, Bombyx mori (2007)
  • Author(s): Tatsuke, et. al.
  • Organizer: International congress of insect biotechnology & industry
  • Place of Presentation: Daegu; Korea
  • Year and Date: 2007-08-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular cloning and characterization of HP1a,HP1b in the silkworm,Bombyx mori (2007)
  • Author(s): Mitsunobu, et. al.
  • Organizer: International congress of insect biotechnology & industry
  • Place of Presentation: Daegu;Korea
  • Year and Date: 2007-08-23
• [Presentation] Moleculer Cloning of Piwi and Aubergine homolog genes from the silkworm,Bombyx mori (2007)
  • Author(s): Tatsuke, et. al.
  • Organizer: International congress of insect biotechnology & industry
  • Place of Presentation: Daegu;Korea
  • Year and Date: 2007-08-23
• [Presentation] カイコにおける相同組換えに関わる遺伝子の機能解析 (2007)
  • Author(s): 門, ら
  • Organizer: 蚕糸学会本会
  • Place of Presentation: 茨城
  • Year and Date: 2007-04-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カイコHPlaの機能解析 (2007)
  • Author(s): 光延, ら
  • Organizer: 蚕糸学会本会
  • Place of Presentation: 茨城
  • Year and Date: 2007-04-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カイコにおけるFANCLのクローニングと機能解析 (2007)
  • Author(s): 管原, ら
  • Organizer: 蚕糸学会本会
  • Place of Presentation: 茨城
  • Year and Date: 2007-04-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 高効率的組換えタンィくク質発現用宿主としてのカイコ系統の開発 (2007)
  • Author(s): 李, ら
  • Organizer: 蚕糸学会本会
  • Place of Presentation: 茨城
  • Year and Date: 2007-04-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 高効率的組換えタンパク質発現用宿主としてのカイコ系統の開発 (2007)
  • Author(s): 李, ら
  • Organizer: 蚕糸学会本会
  • Place of Presentation: 茨城
  • Year and Date: 2007-04-04
• [Presentation] Reciprocal Expression of the Bombyx innexin gene family.Sun Mee Hong, Si Kab Nho, Seok Woo Kang, Jae Man Lee (2007)
  • Author(s): Sun Mee, Hong, Si Kab, Nho, Seok Woo, Kang, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: International Congress of Insect Biotechnology & Industry
  • Place of Presentation: Daegu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular cloning and characterization of HP1a, HP1b in the silkworm, Bombyx mori. Hitoshi Mitsunobu, Hiroaki Mon, Jae Man Lee (2007)
  • Author(s): Hitoshi, Mitsunobu, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: International Congress of Insect Biotechnology & Industry
  • Place of Presentation: Daegu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Moleculer Cloning of Piwi and Aubergine homolog genes from the silkworm, Bombyx mori.Tsuneyuki Tatsuke, Kousuke Sakashita, Ryouhei Sugahara, JaeMan Lee (2007)
  • Author(s): Tsuneyuki, Tatsuke, Kousuke, Sakashita, Ryouhei, Sugahara, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: International Congress of Insect Biotechnology & Industry
  • Place of Presentation: Daegu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular cloning and characterization of histone methyltransferase Su(var)3-9 in Bombyx mori (2007)
  • Author(s): Natsumi, Takeno, Hitoshi, Mitsunobu, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: International Congress of Insect Biotechnology & Industry
  • Place of Presentation: Daegu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Virulence of phospholipase C mutants of Psudomonas Aeruginosa against Bombyx mori (2007)
  • Author(s): Kazuhiro, Iiyama, Yuuka, Chieda, Jae Man, Lee, Takahiro, Kusakabe Chisa, Yasunaga-Aoki, Susumu, Shimizu
  • Organizer: International Congress of Insect Biotechnology & Industry
  • Place of Presentation: Daegu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Exotoxin is an important virulence factor of Pseudomonas aeruginosa against Bombyx mori (2007)
  • Author(s): Yuuka, Chieda, Kazuhiro, liyama, Jaeman, Lee, Takahiro, kusakabe, Chisa, Yasunaga-Aoki, Susumu, Shimizu
  • Organizer: International Congress of Insect Biotechnology & Industry
  • Place of Presentation: Daegu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterizing of novel protein similar to HSF(heat shock transcription factor) in the silkworm (2007)
  • Author(s): Bombyx, Mori
  • Organizer: International Congress of Insect Biotechnology & Industry
  • Place of Presentation: Daegu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] DNA repair and recombination in insect cells (2006)
  • Author(s): Takahiro, Kusakabe, Hiroaki, Mon, Haruna, Tsukioka, Lee, JaeMan, Yutaka, Kawaguchi
  • Organizer: International Symposium on Insect Genetics & Genomics
  • Place of Presentation: Hyderabad ; India
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Double-strand break repair by homologous recombination in silkworm cells (2006)
  • Author(s): Hiroaki, Mon, Ryohei, Sugahara, JaeMan, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto ; Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular cloning and characterization of HP1a, HP1b in the silkworm, Bombyx mori (2006)
  • Author(s): Hitoshi, Mitsunobu, Hiroaki, Mori, Masateru, Takahashi, Jac Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto, Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] A proteomic approach to silkworm proteins responding to g-irradiation (2006)
  • Author(s): Hiroko, Yoshida, Masateru, Takahashi, Pingbo, Zhang, Yoshinon, Ueno, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahtro, Kusakabe
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto ; Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular cloning and characterization of FANCD2 in Bombyx mori Ryohei Sugahara (2006)
  • Author(s): Hiroaki, Mon, Jae Man, Lee, Takahiro, Kusakabe, Yutaka, Kawaguchi
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto ; Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular cloning and characterization of HSP90 cochaperones in the silkworm Bombyx mori (2006)
  • Author(s): Jun, Yamashita, Yoshitaka, Miyagawa, Hiroaki, Mon, Hitoshi, Mistunobu, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto ; Japan
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Cellular response to DNA double strand beraks(DSBs) in cultured silkworm cells (2006)
  • Author(s): Takahiro, Kusakabe, Hiroaki, Mon, Masateru, Takahashi, JaeMan, Lee, Yutaka, Kawaguchi
  • Organizer: The Workshop on Lepidopteran Molecular Biology and Genetics
  • Place of Presentation: Crete ; Greek
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Double-strand break repair by homologous recombination in silkworm cells (2006)
  • Author(s): Hiroaki, Mori, Ryohei, Sugahara, JaeMan, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: The Workshop on Lepidopteran Molecular Biology and Genetics
  • Place of Presentation: Crete ; Greek
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] A Large Amount of dsRNA affects on Gene Expression in Cultured Silkworm Cells (2006)
  • Author(s): Kousuke, Sakashita, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: Asta-Pacific Congress of Sericulture & Insect Biotechnology
  • Place of Presentation: Sangju ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Proteomic Analysis of Silkworm Proteins Responding to DNA Damage Induced by Ionizing Radiation (2006)
  • Author(s): Hiroko, Yoshida, Masateru, Takahashi, Hiroaki, Mon, Pingbo, Zhang, Yoshinori, Ueno, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: Asia-Pacific Congress of Sericulture & Insect Biotechnology
  • Place of Presentation: Sangiu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular Cloning and Functional Characterization of the Fanconi Anemia Protein, FANCD2 in Bombyx mori (2006)
  • Author(s): Ryohei, Sugahara, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: Asia-Pacific Congress of Sericulture & Insect Biotechnology
  • Place of Presentation: Sangiu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular Cloning and Characterization of Hsp70, Hsc70-4, and Hsp40 in the silkworm (2006)
  • Author(s): Bombyx, mori Jun, Yamashita, Yoshitaka, Miyagawa, Hitoshi, Mitsunobu, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: Asia-Pacific Congress of Sericulture & Insect Biotechnology
  • Place of Presentation: Sangiu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Bombyx mori as a model host for Pseudomonas aerugmosa infection (2006)
  • Author(s): Kazuhiro, Yuuka, Chieda, Jaeman, Lee, Takahiro, kusakabe, Chisa, Yasunaga-Aoki, Susumu, Shimizu
  • Organizer: Asia-Pacific Congress of Sericulture & Insect Biotechnology
  • Place of Presentation: Sangju ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] uvrC is necessary for Pseudomonas aeruginosa PAO1 to be highly virulent in Bombyx mori (2006)
  • Author(s): Yuuka, Chieda, Kazuhiro, Iiyama, Jaeman, Lee, Takahiro, kusakabe, Chisa, Yasunaga-Aoki, Susumu, Shimizu
  • Organizer: Asia-Pacific Congress of Sericulture & Insect Biotechnology
  • Place of Presentation: Sangiu ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Comparison of productive and non-productive Bombyx mori nucleopolyhedrovirus infection in selected insect cell lines (2006)
  • Author(s): Naoya, Kawakami, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: Asia-Pacific Congress of Sericulture & Insect Biotechnology
  • Place of Presentation: Sangm ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Establishment of fluorescent proteins-fusion systems in the silkworm, Bombyx mori (2005)
  • Author(s): Hitoshi, Mitsunobu, Hiroaki, Mon, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: 5th Asia-Pacific Congress of Entomology
  • Place of Presentation: Jeju ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Comparison of gene transfer efficiency into insect cells and tissues with different methods (2005)
  • Author(s): Jae Man, Lee, Masateru, Takahashi, Hiroaki, Mon, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: 5th Asia-Pacific Congress of Entomology
  • Place of Presentation: Jeju ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Double strand break repair by homologous recombination in silkworm cells (2005)
  • Author(s): Hiroaki, Mon, Ryohei, Sugahara, Takahiro, Kusakabe, Yutaka, Kawaguchi
  • Organizer: 5th Asia-Pacific Congress of Entomology
  • Place of Presentation: Jeju ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Construction of silkworm gene silencing systems using bacurovirus mediated gene transfer (2005)
  • Author(s): Yoshito, Kojin, Jae Man, Lee, Takahiro, Kusakabe, Yutaka, Kawaguchi
  • Organizer: 5th Asia-Pacific Congress of Entomology
  • Place of Presentation: Jeju ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Construction of Tet-on gene expression system in silkworm, Bombyx mori (2005)
  • Author(s): Noriko, Karasaki, Hiroaki, Mon, Takahiro, Kusakabe, Yutaka, Kawaguchi, Katumi, Koga
  • Organizer: 5th Asia-Pacific Congress of Entomology
  • Place of Presentation: Jeju ; Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The expression profiles of silkworm RAD51 and DMC1 genes during the process of meiotic recombination (2005)
  • Author(s): Yukiko, Ishikawa, Yoshiaki, Kosaka, Hiroaki, Mon, Jae Man, Lee, Yutaka, Kawaguchi, Takahiro, Kusakabe
  • Organizer: 5th Asia-Pacific Congress of Entomology
  • Place of Presentation: Jeju ; Korea
  • Description: 「研究成果報告書概要(欧文)」より