Structure and function analysis of novel meta-cleavage enzyme for the creation of the enzyme to degrade recalcitrant and environment-polluting compounds

• Project/Area Number: 17380056
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Shibaura Institute of Technology
• Principal Investigator: OMORI Toshio Shibaura Institute of Technology, Engineering graduate course, Professor (20011984)
• Co-Investigator(Kenkyū-buntansha): 羽部 浩 (独立法人)産業技術総合研究所, 研究員 (20313075)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥11,060,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 2005: ¥6,100,000 (Direct Cost: ¥6,100,000)
• Keywords: marine bacteria / meta-cleavage / carbazole / dibenzofuran / recalcitrant compound / x-ray crystallograph / X線結晶構造 / 2サブユニット / Pseudomonas resinovorance CA10 / Rodoccus sp. DFA3 / 海洋

Research Abstract

Currently, research on the biodegradation of recalcitrant chemicals is mainly concentrated on microorganisms isolated from soils and activated sludge. Studies on marine bacterial genes and enzymes involved in biodegradation of xenobiotics such as carbazole and dioxins are very rare. However, accidents caused by damage to tankers, or the inflow of recalcitrant molecules from rivers, have created pollution in seas that requires environmental remediation.
The concentration of bacteria in the sea is very low, and it is very difficult to culture these bacteria because quantities of trace elements present in the seawater affect the growth of marine bacteria. To overcome of these problems, it is necessary to concentrate the seawater to enable bacterial screening. By manipulating the composition of the isolation medium, we succeeded in isolating organisms from seawater that can degrade carbazole and dibenzofuran. We isolated new bacterial carbazole degraders, classified as Lysobacter sp. strain s OC6 and OC7, which show very low homology to reported bacteria and probably belong to a new order.
To obtain genes from these marine bacteria that may be involved in the degradation of carbazole, genes from soil bacterial enzymes participating in the degradation of carbazole were used as a probe for Southern hybridization, but no hybridization was achieved. Using the shotgun cloning method, we successfully obtained the genes from marine bacteria that are involved in the degradation of recalcitrant compounds.
The meta-cleavage enzyme involved in the degradation of carbazole and dibenzofuran consists of two subunits, but the three-dimensional (3D) structure of the enzyme is not yet established. However, this information is required to elucidate the 3D structure of the enzymes in order to construct new enzymes that have new substrate specificities and high activity. The meta-cleavage enzyme from Pseudomonas resinovorans CA10, which has two subunits, was purified and crystallized, and the 3D structure was established by the SIRS method. Elucidation of the 3D meta-cleavage enzyme structure of marine bacterial strains is now in progress. These results will be used to construct enzymes with new substrate specificities and activities by comparing the enzymes of terrestrial and marine bacteria.

Research Products

• [Journal Article] Transcriptional regulation of the sulfate-starvation-induced gene sfnA by a o^<54>-dependent activator of Pseudomonas putida (2007)
  • Author(s): Habe, H., Kouzuma, A., Endoh, T., Omori, T., Yamane, H. and Nojiri, H.
  • Journal Title: Microbiology 153 Pages: 3091-3098
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The ptsP gene encoding the PTS family protein EL^<Ntr> is essential for dimethyl sulfone utilization by Pseudomonas putida (2007)
  • Author(s): Kouzuma, A., Endoh, T., T., Omori, Nojiri, H., Yamane, H. and Habe, H.
  • Journal Title: Microbiol Lett 275 Pages: 175-181
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of the sulfate-starvation-induced gene sfnA by a am-dependent activator of Pseudomonas putida (2007)
  • Author(s): Habe, H., Kouzuma, A., Endoh, T., Omori, T., Yamane, H., Nojiri, H
  • Journal Title: Microbiology 153 Pages: 3091-3098
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The ptsP gene encoding the PTS family protein EL^<Ntr> is essential for dimethyl sulfone utilization by Pseudomonas putida (2007)
  • Author(s): Kouzuma, A., Endoh, T., T., Omori, Nojiri, H., Yamane, H., Habe, H
  • Journal Title: FEMS Microbiol Lett 275 Pages: 175-181
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transcriptional regulation of the sulfate-starvation-induced gene sfnA by a σ^54-dependent activator of Pseudomonas nutida (2007)
  • Author(s): Habe Hirosh
  • Journal Title: Microbiology 53 Pages: 3091-3098
  • Peer Reviewed
• [Journal Article] The ptsP gene encodjhg the PTS family protein EL^Ntr is essential fbrdimethvl sulfone utilization by Pseudomonas putida (2007)
  • Author(s): Kouzuma Atsushi
  • Journal Title: Microbiol Lett 275 Pages: 175-181
  • Peer Reviewed
• [Journal Article] Detection of a bacterial group within the phylum Chloroflexi and reductive-dehalogenase-homologous genes in pentachlorobenzene-dechlorinating estuarine sediment from the Arakawa River,Japan (2006)
  • Author(s): Santoh, K., Kouzuma, A., Ishizaki, R., Iwata, K., Shimura, M., Hayakawa, T., Hoaki, T., Nojiri, H., Omori, T., Yamane, H. and Habe, H.
  • Journal Title: Microbes Environ 21 Pages: 154-162
  • NAID: 110004809523
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Improving the catalytic efficiency of meta-cleavage product hydrolase(CumD) from Pseudomonas fluorescens IP01 (2006)
  • Author(s): So-Young, J., Fushinobu, S., Nojiri, H., Omori, T., Shoun, H. and Wakagi T.
  • Journal Title: Biochimica et Biophysica Acta 1764 Pages: 1159-1166
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differentiation of carbazole catabolic operons by replacement of the regulated promoter viatransposition of an insertion sequence (2006)
  • Author(s): Miyakoshi, M., Urata, M., Habe, H., Omori, T., Yamane, H. and Nojiri, H.
  • Journal Title: Journal of Biological Chemistry 281 Pages: 8450-8457
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characterization of the replication,maintenance,and transfer features of the Inc P-7 plasmid pCAR1,which carries genes involved in carbazole and dioxin degradation (2006)
  • Author(s): Shintani, M., Yano, H., Habe, H., Omori, T., Yamane, H., Tsuda, M. and Nojiri, H.
  • Journal Title: Appl.Environ.Microbiol. 72 Pages: 3206-3216
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Plasmid pCAR3 contains multiple gene sets involved in the conversion of carbazole to anthranilate (2006)
  • Author(s): Urata, M., Uthimura, H., Noguchi, H., Sakaguchi, T., Eto, K., Habe, H., Omori, T., Yamane, H. and Nojiri, H.
  • Journal Title: Appl.Environ.Microbiol. 72 Pages: 3198-3205
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differentiation of carbazole catabolic operons by replacement of the regulated promoter via transposition of an insertion sequence (2006)
  • Author(s): Miyakoshi, M., Urata, M., Habe, H., Omori, T., Yamane, H., Nojiri, H
  • Journal Title: Journal of Biological Chemistry 281 Pages: 8450-8457
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Improving the catalytic efficiency of meta-cleavage product hydrolase(CumD) from Pseudomonas fluorescens IPOI (2006)
  • Author(s): So-Young, J., Fushinobu, S. Nojiri, H., Omori, T., Shoun, H., Wakagi, T
  • Journal Title: Biochimica et Biophysica Acta 1764 Pages: 1159-1166
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Detection of a bacterial group within the phylum Chloroflexi and reductive-dehalogenase-homologous genes in pentachlorobenzene-dechlorinating estuarine sediment from the Arakawa River, Japan (2006)
  • Author(s): Santoh, K., Kouzuma, A., Ishizaki, R., Iwata, K., Shimura, M., Hayakawa, T., Hoaki, T., Nojiri, H., Omori, T., Yamane, H., Habe, H
  • Journal Title: Microbes Environ 21 Pages: 154-162
  • NAID: 110004809523
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of the replication, maintenance, and transfer features of the Inc P-7 plasmid pCAR1, which carries genes involved in carbazole and dioxin (2006)
  • Author(s): Shintani, M., Yano, H., Habe, H., Omori, T., Yamane, H., Tsuda, M., Nojiri, H
  • Journal Title: Appl. Environ. Microbiol 72 Pages: 3206-3216
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Plasmid pCAR3 contains multiple gene sets involved in the conversion of carbazole to anthranilate degradation (2006)
  • Author(s): Urata, M., Uthimura, H., Noguchi, H., Sakaguchi, T. Eto, K., Habe, H., Omori, T., Yamane, H., Nojiri, H
  • Journal Title: Appl. Environ. Microbiol 72 Pages: 3198-3205
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Detection of a bacterial group within the phylum Chloroflexi and reductive-dehalogenase-homologous genes in pentachlorobenzene-dechlorinating estuarine sediment from the Arakawa River, Japan. (2006)
  • Author(s): Santoh, K., Kouzuma, A., Ishizaki, R., Iwata, K., Shimura, M., Hayakawa, T., Hoaki, T., Nojiri, H., Omori, T., Yamane, H., Habe, H.
  • Journal Title: Microbes Environ 21, Pages: 154-162
  • NAID: 110004809523
• [Journal Article] Improving the catalytic efficiency of meta-cleavage product hydrolase(CumD) from Pseudomonas fluorescens IP01. (2006)
  • Author(s): So-Yooung, J., Fushinobu, S., Nojiri, H., Omori, T., Shoun, H., Wakagi T.
  • Journal Title: Biochimica et Biophysica Act 1764 Pages: 1159-1166
• [Journal Article] Differentiation of carbazole catabolic operons by replacement of the regulated promoter via transposition of an insertion sequence. (2006)
  • Author(s): Miyakoshi, M., Urata, M., Habe, H., Omori, T., Yamane, H., Nojiri, H.
  • Journal Title: Journal of Biological Chemistry 281 Pages: 8450-8457
• [Journal Article] Characterization of the replication, maintenance, and transfer features of the Inc P-7 plasmid pCAR1, which carries genes involved in carbazole and dioxin degradation. (2006)
  • Author(s): Shintani, M., Yano, H., Habe, H., Omori, T., Yamane, H., Tsuda, M., Nojiri, H.
  • Journal Title: Appl. Environ. Microbiol. 72, Pages: 3206-3216
• [Journal Article] Plasmid pCAR3 contains multiple gene sets involved in the conversion of carbazole to anthranilate. (2006)
  • Author(s): Urata, M., Uthimura, H., Noguchi, H., Sakaguchi, T., Eto, K., Habe, H., Ommori, T., Yamane, H., Nojiri, H.
  • Journal Title: Appl. Environ. Microbiol. 72, Pages: 3198-3205
• [Journal Article] Characterization of [3Fe-4S] ferredoxin DbfA3, which functions in the angular dioxygenase system of Terrabacter sp. strain DBF63. (2005)
  • Author(s): Takagi, T., Habe, H., Yoshida, T., Yamane, K., Omori, T., Nojiri H.
  • Journal Title: Appl.Microbiol.Biotechnol 68 Pages: 336-345
• [Journal Article] Structure of terminal oxygenase component of angular dioxygenase, carbazole 1,9a-dioxygenase. (2005)
  • Author(s): Nojiri, H., Ashikawa, Y, Noguchi, H., Omori, T., et al.
  • Journal Title: J.Mol.Biol. 351 Pages: 355-370
• [Journal Article] The fluorine catabolic linear plasmid in Terrabacter sp. strain DBF63 carries the β-katoadipate pathway genes, pcaRHGBDCFIJ, also found in proteobacteria. (2005)
  • Author(s): Habe, H., Chung, J., Ishida, A., Kasuga, Yamane, H., Omori, T.et al.
  • Journal Title: Microbiology 153 Pages: 3713-3722
• [Journal Article] Molecular technology of xenobiotic-degrading bacteria and their catabolic genes in bioremediation. (2005)
  • Author(s): Inoue, K., Widada, J., Omori, T., Nojiri, H.
  • Journal Title: Science Publishers Pages: 1-30
• [Journal Article] Crystalization and preliminary Xray-diffraction analysis of the electron-trnsfer complex between the terminal oxygenase component and ferredoxin in the Rieke non-haem iron oxygenase system carbazole 1,9-dioxygenase. (2005)
  • Author(s): Ashikawa, Y., Fujimoto Z., Noguchi H., Habe H., Omori T., Yamane H., Nojiri H.
  • Journal Title: Acta Cryst. F61 Pages: 577-580
• [Journal Article] Large plasmid pCAR2 and class II transposon Tn 4676 are functionl mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria. (2005)
  • Author(s): Shintanl M., Yoshida T., Habe H., Omori T., Nojiri H.
  • Journal Title: Appl.Mlcrobiol.Biotechnol. 67 Pages: 370-382
• [Presentation] カルバゾール分解海洋性細菌CAR-SF株由来メタ開裂酵素の機能解析 (2008)
  • Author(s): 岩田 健一
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-28
• [Presentation] 新目に属するカルバゾール分解海洋性細菌OC6S株の報告と諸性質 (2008)
  • Author(s): 大森 俊雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-28
• [Presentation] カルバゾール分解新規海洋性細菌の探索と機能解析 (2008)
  • Author(s): 前田 臨太郎
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-28
• [Presentation] 海洋性細菌Lysobacter sp.OC7株が有する難分解性化合物分解系遺伝子の解析 (2008)
  • Author(s): 永嶋 寛
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-28
• [Presentation] Sphingomonas sp.KA1株の変異導入メタ開裂酵素の結晶化 (2008)
  • Author(s): 島田 義己
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-27
• [Presentation] カルバゾール分解細菌由来2サブユニットメタ開裂酵素の小サブユニットの機能解析 (2008)
  • Author(s): AZHAM ZULKHARNAIN
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-27
• [Presentation] Sphingomonas sp.KA1株の変異導入メタ開裂酵素の結晶化 (2008)
  • Author(s): 島田 義己, Azham bin Zulkarnain, 岩田 健一, 大森 俊雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カルバゾール分解細菌由来2サブユニットメタ開裂酵素の小サブユニットの機能解析 (2008)
  • Author(s): Azham bin Zulkarnain, 岩田 健一, 大森 俊雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カルバゾール分解海洋性細菌CAR-SF株由来メタ開裂酵素の機能解析 (2008)
  • Author(s): 岩田 健一, 永嶋 寛, Azham bin Zulkarnain, 布施 博之, 大森 俊雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 新目に属するカルバゾール分解海洋性細菌OC6S株の報告と諸性質 (2008)
  • Author(s): 大森 俊雄, 前田 臨太郎, 岩田 健一
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カルバゾール分解新規海洋性細菌の探索と機能解析 (2008)
  • Author(s): 前田 臨太郎, 永嶋 寛, 岩田 健一, 大森 俊雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 海洋性細菌Lysobacter sp.OC7株カミ有する難分解陸化合物分解系遺伝子の解析 (2008)
  • Author(s): 永嶋 寛, 前田 臨太郎, 岩田 健一, 大森 俊雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression, purification, and characterization of a meta-cleavage enzyme from a marine bacterium, strain CAR-SF (2008)
  • Author(s): Iwata, K., Nagashima, H., Zulkharnain, A., b., Fuse, H., Omni, T
  • Organizer: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Isolation and characterization of novel carbazole-degrading marine bacteria (2008)
  • Author(s): Maeda, R., Nagashima, H., Iwata, K., Omori, T
  • Organizer: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional analysis of the small subunit composed of two subunits of the meta-cleavage enzyme from carbazole-degrading bacteria (2008)
  • Author(s): Zulkhamain, A., b., Iwata, K., Omori, T
  • Organizer: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterization of the carbazole-degrading marine bacterium OC6S that belongs to a new order of bacteria (2008)
  • Author(s): Omori, T., Maeda, R., Iwata, K
  • Organizer: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Gene analysis of the marine bacterium Lysobacter sp. OC7 involved in the degradation of recalcitrant compounds (2008)
  • Author(s): Nagashima, H., Maeda, R., Iwata, K., Omori, T
  • Organizer: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Nagoya
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 難分解性化合物分解新規海洋性細菌の探索と機能解析 (2007)
  • Author(s): 前田 臨太郎, 永嶋 寛, 布施 博之, 羽部 浩, 野尻 秀昭, 岩田 健一, 大森 俊雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 海洋性細菌CAR-SF由来PAHsの分解経路とメタ開裂酵素の研究 (2007)
  • Author(s): 永嶋 寛, Azham bin Zulkarnain, 秋元 篤史, Onruthai Pinyakong, 布施 博之, 岩田 健一, 羽部 浩, 野尻 秀昭, 大森 俊雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 海洋性細菌CAR-SF株が有するカルバゾール分解系遺伝子の解析 (2007)
  • Author(s): Azham bin Zulkarnain, 秋元 篤史, 永嶋 寛, Onruthai Pinyakong, 布施 博之, 岩田 健一, 羽部 浩, 野尻 秀昭, 大森 俊雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Biodegradation of dioxins in contaminated soil by Novosphingobium sp.Strain KA1 (2007)
  • Author(s): 斎木 祐子, 高稿 正士, 川本 八千代, 横田 豊一, 野尻 秀昭, 大森 俊雄
  • Organizer: General Meeting of American Society for Microbiology
  • Place of Presentation: カナダ
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Biodegradation of dioxins in contaminated soil by Novosphingobium sp. Strain KA1 (2007)
  • Author(s): Saiki, Y., Takahasi, M., Kawamoto, Y., Yokota, T., Nojiri, H., Omori, T
  • Organizer: General Meeting of American Society for Microbiology
  • Place of Presentation: Canada
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Expression, purification, and characterization of the meta-cleavage enzymes and the meta-cleavage hydrolase from Sphingomonas sp. KA1 (2007)
  • Author(s): Zulkharnain, A., b., Akimoto, A., Nagashima, H., Pinyagong, O., Fuse, H., Iwata, Nojiri, H., Omori, T
  • Organizer: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Tokyo
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Isolation and nucleotide sequences of carbazole catabolic genes from a marine bacterium, strain CAR-SF (2007)
  • Author(s): Zulldiamain. A., b., Akimoto, A., Nagashima, H., Pinyagong, O., Fuse. H., Iwata., K., Nojiri., H., Omori, T
  • Organizer: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Tokyo
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Isolation and characterization of novel recalcitrant compounds that can be degraded by marine bacteria (2007)
  • Author(s): Maeda, R., Nagashima, H., Fuse, H., Habe, H., Nojiri, H., Omori, T
  • Organizer: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Tokyo
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 難分解性化合物分解に関与する2サブユニットのメタ解裂酵素の諸性質の比較 (2006)
  • Author(s): Azham bin Zulkarnain, 神谷 淳, 岩田 健一, 羽部 浩, 野尻 秀昭, 大森 俊雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Biotechnology research in Japan (2006)
  • Author(s): 大森 俊雄
  • Organizer: The symposium on biotechnology for energy security
  • Place of Presentation: インドネシア
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Development of monitoring system of Pseudomonas resinovorans strain CA10 for use in bioremediation of carbazole and 2,3-dichlorodibenzo-p-dioxin (2006)
  • Author(s): 岩田 健一, 大森 俊雄
  • Organizer: The symposium on biotechnology for energy security
  • Place of Presentation: インドネシア
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular techniques of Xenobiotic-degrading bacteria and their catabolic genes in bioremediation (2006)
  • Author(s): Jaka Widada, 野尻 秀昭, 大森 俊雄
  • Organizer: International Symposium on Advanced Environmental Monitoring
  • Place of Presentation: ドイツ
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Comparison of the characteristics of meta-cleavage enzymes consisting of two subunits that are involved in the degradation of recalcitrant chemicals (2006)
  • Author(s): Zulkhamain, A., b., Kamiya, J., Iwata, K., Habe, H., Nojiri, H., Omori., T
  • Organizer: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Biotechnology research in Japan (2006)
  • Author(s): Omori, T
  • Organizer: The symposium on biotechnology for energy security
  • Place of Presentation: Indonesia
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Development of monitoring system ofPseudomonas resinovorans strain CA10 for use in bioremediation of carbazole and 2, 3-dichlorodibenzo-p-dioxin (2006)
  • Author(s): Iwata, K., Omori, T
  • Organizer: The symposium on biotechnology for energy security
  • Place of Presentation: Indonesia
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Development of monitoring system of Pseudomonas resinovorans strain CA10 for use in bioremediation of carbazole and 2, 3-dichlorodibenzo-p-dioxin (2006)
  • Author(s): Widada, J., Nojiri., H., Omori, T
  • Organizer: International Symposium on Advanced Environmental Monitoring
  • Place of Presentation: Germany
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] バイオフィルムの基礎と制御 (2007)
  • Author(s): 大森 俊雄, 他
  • Total Pages: 432
  • Publisher: (株)エヌ・ティー・エス
  • Description: 「研究成果報告書概要(和文)」より