• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Pathogenesis of BSE and highly sensitive isolation of BSE agent

Research Project

Project/Area Number 17380174
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionThe University of Tokyo

Principal Investigator

ONODERA TAKASBI  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor (90012781)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Yoshitsugu  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor (00173922)
SAEKI KEIICHI  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant (10311630)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2007: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2006: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2005: ¥5,700,000 (Direct Cost: ¥5,700,000)
Keywordsprion / BSE / scrapie / food safety / gene
Research Abstract

The chain reaction of BSE epidemics in the UK and Europe and subsequent emergence of vCJD in young adults and teenagers have raised concerns and highlighted the importance of risk assessment in the food chain. Recently, several highly sensitive methods for detecting priors have been developed. Representative of these is PMCA.
Originally developed by Claudio Soto and his colleagues, PMCA has been a hot topic of debate in prion meetings all over the world. A broad spectrum of PrP^Sc species have now been successfully amplified using PMCA, including CWD, mouse-adapted scrapie, and BSE. Studies with human sporadic and variant CJD cases show that PMCA amplification efficiency is tightly controlled by the PrP^C substrate genotype at codon 129. PMCA appears to overcome the species barrier encountered during cross-species transmission more rapidly than in vivo. By "forcing" the technique with lower dilutions of the PrP^Sc seed and more amplification rounds, mouse Chandler PrP^Sc can now convert hamster PrP^C or cervid PrP^C, a conversion that might be observable in vivo but only with extremely long incubation periods. Castilla J. showed that using PMCA, PrP^Sc was generated from the healthy brains of 11 different species, including bank voles, mice, cattle, humans, sheep and rabbits, generating a variety of electrophoretic profiles. PMCA was able to detect PrP^Sc in as little as 1 μL of blood from an asymptomatic prion-infected mouse. Given the increasing evidence of human to human transmission via blood products, scientists are waiting for PMCA to be incorporated into a reliable test with the ability to identify blood donors that are asymptomatic carriers. Further advances in amplification technology are to be expected and the replacement of PrP^C by recombinant PrP as a substrate as well as the use of intermittent shaking rather than sonication should circumvent some of the difficulties in the near future.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (20 results)

All 2008 2007 2006 2005

All Journal Article (20 results) (of which Peer Reviewed: 6 results)

  • [Journal Article] PrPsc level and incubation time in a transgenic mouse model expressing Borna disease virus phosphoprotein after intracerebral infection.2008

    • Author(s)
      Sakudo, A, and Onodera T.
    • Journal Title

      Neurosci. Lett. 431

      Pages: 81-85

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] PrPsc level and incubation time in a transgenic mouse model expressing Borna disease virus phosphoprotein after intracerebral infection2008

    • Author(s)
      Sakudo, A, Onodera T.
    • Journal Title

      Neurosci. Lett. 431

      Pages: 81-85

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] PrPsclevel and incubation time in a transgenic mouse model espressing Borna disease virus phosphoprotein after intracerebral prion infection2008

    • Author(s)
      Sakudo A, and Onodera T.
    • Journal Title

      Neurosci Lett 431

      Pages: 81-85

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A monoclonal antibody (ID12) defines el distribution patterns of prion protein (PrP) as granudes in nucleus.2007

    • Author(s)
      Sakudo s, Onodera T.
    • Journal Title

      Biochem Biophys Res Commun 366

      Pages: 657-663

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Octapeptide region of prion protein (PrP) is required at an early stage for production of abnormal prion protein in PrP-deficient neuronal cell line.2007

    • Author(s)
      Sakudo s, Onodera T.
    • Journal Title

      Biochem Biophys Res Commun 365

      Pages: 164-169

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A monoclonal antibody (ID12) defines nucleus. novel distribution patterns of prion protein (PrP) as granudes in nucleus2007

    • Author(s)
      Sakudo S, Onodera T.
    • Journal Title

      Biochem Biophys Res Commun 366

      Pages: 657-663

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Octapeptide region of prion protein (PrP) is required at an early stage for production of abnormal prion protein in PrP-deficient neuronal cell line2007

    • Author(s)
      Sakudo S, Onodera T.
    • Journal Title

      Biochem Biophys Res Commun 365

      Pages: 164-169

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A monoclonal antibody(ID12)defines novel distribution patterns of prion protein(PrP)as granudes in nucleus2007

    • Author(s)
      Sakudo A, and Onodera T.
    • Journal Title

      Biochem Biophys Res Commun 366

      Pages: 657-663

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Octapeptide region of prion protein(PrP)is required at an early stage for productio of abnomal prion protein in PrP-deficient neuronal cell line2007

    • Author(s)
      Sakudo A, and Onodera T.
    • Journal Title

      Biochem Biophys Res Commun 365

      Pages: 164-169

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The truncated 23-230 form of the prion protein localizes to the nuclei of inducible cell lines independently of its nuclear localization signals in not cytotoxic.2006

    • Author(s)
      Crozet, C., Onodera T.
    • Journal Title

      Biosci. Boitechnol. Biochem. 70

      Pages: 1578-1583

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Bovine spongiform encephalopathy in Japan : History and recent studies on oxidative stress in prion diseases.2006

    • Author(s)
      Onodera, T., Sakudo A.
    • Journal Title

      Microbiology and Immunology 50(8)

      Pages: 565-578

    • NAID

      10018168090

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Recent advances in clarifying prion protein functions using knockout mice and derived cell lines2006

    • Author(s)
      Sakudo, A., Onodera T.
    • Journal Title

      Medicinal Chemistry 6

      Pages: 589-601

    • Related Report
      2006 Annual Research Report
  • [Journal Article] BSE situation and establishment of Food Safety Commission in Japan2006

    • Author(s)
      Onoder T., Kim CK
    • Journal Title

      Journal of Veterinary Science 7

      Pages: 1-11

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Fusion of Doppel to octapeptide repeat and N-terminal half of hydrophobic region of prion protein confers resistance to serum deprivation2006

    • Author(s)
      Lee DC, Onodera T.
    • Journal Title

      Microbiology and Immunology 50

      Pages: 203-209

    • NAID

      10017327601

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Poliovirus infection of prion-deficient neuronal cells.2005

    • Author(s)
      Baj, A., Onodera T.
    • Journal Title

      J.Neurovirol. 11

      Pages: 237-246

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Octapeptide repeat region and N-terminal half of hydrophobic region of prion protein (PrP) mediates PrP-dependent activation of superoxide dusmutase.2005

    • Author(s)
      Sakudo, A., Onodera T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 326

      Pages: 600-606

    • Related Report
      2005 Annual Research Report
  • [Journal Article] PrP cooperates with STI1 to regulate SOD activity in PrP deficient neuronal cell line.2005

    • Author(s)
      Sakudo, A., Onodera T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 328

      Pages: 14-19

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Activation of phopsphatidylinositol 3-kinase by cellular prion protein (PrPc) and its role in cell survival.2005

    • Author(s)
      Vassalo, N., Onodera T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 332

      Pages: 75-82

    • Related Report
      2005 Annual Research Report
  • [Journal Article] BSE感染動物の危険部位と安全対策2005

    • Author(s)
      小野寺節
    • Journal Title

      食品衛生学雑誌 46

      Pages: 323-326

    • NAID

      10031169353

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Infection-route independent accumulation of splenic abnormal prion protein.2005

    • Author(s)
      Inoue, Y., Onodera T.
    • Journal Title

      Jpn.J.Inf.Dis. 58

      Pages: 78-82

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi