Roles and regulation of endoderm stem cells in hereditary liver disease

• Project/Area Number: 17390100
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human genetics
• Research Institution: Kumamoto University
• Principal Investigator: ENDO Fumio Kumamoto University, Faculty of Medical and Pharmaceutical Sciences, Professor, 大学院医学薬学研究部, 教授 (00176801)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Tetsuro Kumamoto University, Faculty of Medical and Pharmaceutical Sciences, Professor, 大学院医学薬学研究部, 教授 (60112405) ; NAKAMURA Kimitoshi Kumamoto University, University, Hospital, Research Associates, 医学部附属病院, 助手 (30336234)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥14,600,000 (Direct Cost: ¥14,600,000); Fiscal Year 2006: ¥6,000,000 (Direct Cost: ¥6,000,000); Fiscal Year 2005: ¥8,600,000 (Direct Cost: ¥8,600,000)
• Keywords: hepatocyte / stem cell / liver injury / regenerative medicine / genetics / amino acid / metabolic disease / 内胚葉 / DNAチップ / 遺伝性疾患

Research Abstract

We attempted to reorganize hepatobiliary system by means of transplantation of progenitor cells derived from salivary gland. Our achievement should be useful for therapy not only hepatic but also for biliary and pancreatic diseases. We transplanted gene modified hepatic cells into model mice for hereditary hepatic disease. Using the cell transplantation, we identified new endodermal stem cells. We established technique to replace damaged hepatocyte of the model mice with gene modified hepatocyte. Furthermore, we found method for separating hepatic progenitor cells from adult. Next, we established method for separating stern cells of liver and pancreas. We can produce hepatic and pancreatic cells in vivo and in vitro. We established method to isolate hepatic and pancreatic cells derived from pluripotent stem cells. In this method, GFP labeled cells were recognized as pancreatic progenitor cells. We investigated gene expressions of progenitor cells isolated by this method. We have got new founding after investigation of gene expression of translation factors.

Research Products

• [Journal Article] solation, tissue localization, and cellular characterization of progenitors derived from adult human salivary glands. (2007)
  • Author(s): Sato A, Okumura K, Matsumoto S, Hattori K, Hattori S, Shinohara M, Endo F
  • Journal Title: Cloning and Stem Cells (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Isolation of tissue progenitor cells from duct-ligated salivary glands of swine. (2007)
  • Author(s): Matsumoto S, Okumura K, Ogata A, Hisatomi Y, Sato A, Hattori K, Matsumoto M, Kaji Y, Takahashi M, Yamamoto T, Nakamura K, Endo F
  • Journal Title: Cloning and Stem Cells (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Isolation of tissue progenitor cells from duct-ligated salivary glands of swine. (2007)
  • Author(s): Matsumoto S, Okumura K, Ogata A, Hisatomi Y, Sato A, Hattori K, Matsumoto M, Kaji Y, Takahashi M, Yamamoto T, Nakamura K, Endo F.
  • Journal Title: Cloning and Stem Cells (In press)
• [Journal Article] Gene expression profiles of homogentisate-treated Fah-/-Hpd-/-mice using DNA microarrays. (2006)
  • Author(s): Tanaka Y., Nakamura K., Matsumoto S., Kimoto Y., Tanoue A., Tsujimoto G., Endo F.
  • Journal Title: Molecular Genetics and Metabolism 89 Pages: 203-209
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Gene expression profiles of homogentisate-treated Fah-/-Hpd-/-mice using DNA microarrays. (2006)
  • Author(s): Tanaka Y., Nakamura K., Matsumoto S., Kimoto Y., Tanoue A., Tsujimoto G., Endo F.
  • Journal Title: Molecular Genetics and Metabolism. 89 Pages: 203-209
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gene expression profiles of homogentisate-treated Fah-/- Hpd-/-mice using DNA microarrays. (2006)
  • Author(s): Tanaka Y., Nakamura K., Matsumoto S., Kimoto Y., Tanoue A., Tsujimoto G., Endo F.
  • Journal Title: Molecular Genetics and Metabolism 89 Pages: 203-209
• [Journal Article] Apoptosis under hypercytokinemia is a possible pathogenesis in influenza-associated ercephalopathy. (2005)
  • Author(s): Nunoi H., Endo F., et al.
  • Journal Title: Pediat.Inter. 47 Pages: 175-179
• [Journal Article] An inplication from clinical and molecular investigations on maple syrup urine disease. (2005)
  • Author(s): Mitsubuchi H., Endo F., et al.
  • Journal Title: J.Nutr. 135
• [Journal Article] Isolation, tissue localization, and cellular characterization of progenitors derived from adult human salivary glands.
  • Author(s): Sato A, Okumura K, Matsumoto S, Hattori K, Hattori S, Shinohara M, Endo F.
  • Journal Title: Cloning and Stem Cells (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation of tissue progenitor cells from duct-ligated salivary glands of swine.
  • Author(s): Matsumoto S, Okumura K, Ogata A, Hisatomi Y, Sato A, Hattori K, Matsumoto M, Kaji Y, Takahashi M, Yamamoto T, Nakamura K, Endo F.
  • Journal Title: Cloning and Stem Cells (in press)
  • Description: 「研究成果報告書概要(欧文)」より