Studies on cell death and survival signals via connexin channels during cell injury

• Project/Area Number: 17390118
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: OYAMADA Masahito Kyoto Prefectural University of Medicine, Department of Pathology and Cell Regulation, Associate Professor (30183255)
• Co-Investigator(Kenkyū-buntansha): HARADA Yoshinori Kyoto Prefectural University of Medicine, Department of Pathology and Cell Regulation, Instructor (10381956)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥14,720,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥1,020,000); Fiscal Year 2007: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2006: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 2005: ¥6,700,000 (Direct Cost: ¥6,700,000)
• Keywords: connexin / gap functions / cell death / cell survival / CALI / multi-photon microscopy / GFP / intercellular communication / 細胞障害 / 心筋梗塞 / 生体分子

Research Abstract

Gap junctions are considered to play an important role in moderating cell death and survival. Genetic approaches to the connexins (Cxs) such as gene knockouts and RNA interference, which ultimately reduce Cx levels in the entire cell, have provided direct evidence that gap junctional intercellular communication is essential for cell survival. However, it is possible that one kind of Cx performs different functions depending on its location and/or a particular stage of temporal events. Such spatiotemporal differences in protein function are difficult to analyze with conventional genetic approaches. In this study, we have developed multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI),that specific gap junctions made of Cx-enhanced green fluorescent protein(EGFP)can be spatiotemporally inactivated by brief laser irradiation. Experiments were performed on a pair of HeLa cells connected via a small gap junction plaque (length, <1 μm)consisting of Cx43-EGFP. While junctional currents were measured using the dual whole-cell voltage-clamp -method, a single point of the Cx43-EGFP plaque was irradiated with 850-nm femtosecond laser light with a laser irradiance of 2.7 MW/cm^2for 380 ms. We found that during the laser irradiation, the gap junction current decreased "○80%. However the same irradiation did not change gap junctional currents between HeLa cell pairs expressing Cx43 tagged with monomeric red fluorescent protein(mRFP),indicating that MP-CALI at a wavelength of 850 nm specifically inactivates gap junctions made of Cx-EGFP. We also found that MPCALI can inhibit dye coupling between specifically selected cell pairs. MP-CALI of Cx43-EGFP showed a laser power dependent decrease of gap junction current i.e., the threshold laser power for current reduction is between 1.0 and 1.3 MW/cm2. Reactive oxygen species (ROS) scavengers, edaravone and sodium azide, impaired the MP-CALI response, indicating involvement of ROS in MP-CALI. Thus, MP-CALI could be useful for the elucidation of new Cx functions.

Research Products

• [Journal Article] Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo (2008)
  • Author(s): Nakano Y, Ovamada M, Dai P, Nakagami T, Kinoshita S, Takamatsu T.
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 93-104
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo (2008)
  • Author(s): Nakano, Y., Ovamada, M., Dai, P., Nakagami, T., Kinoshita, S., Takamatsu, T
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 93-104
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo (2008)
  • Author(s): Nakano Y, Oyamada M, Dai P, Nakagami T, Kinoshita S, Takamatsu T
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 93-104
  • Peer Reviewed
• [Journal Article] 多光子CALIによる生細胞内での蛋白質機能の時空間選択的阻害 (2007)
  • Author(s): 小山田 正人, 高松 哲郎
  • Journal Title: 病理と臨床 25 Pages: 553-559
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 傷害心のカルシウム動態異常とギャップ結合の変化 (2007)
  • Author(s): 田中 秀央, 小山田 正人, 辻井 英治, 万井 弘基, 中上 拓男, 藤原 克次, 高松 哲郎
  • Journal Title: Jpn J Electrocardiology 27 Pages: 230-236
  • NAID: 10019492041
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Spatiotemporal inactivation of protein function in the living cells using multiphoton-evoked chromophore-assisted laser inactivation. (in Japanese) (2007)
  • Author(s): Ovamada, M., Takamatsu, T
  • Journal Title: Pathology and Clinical Medicine 25 Pages: 553-559
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Changes in the intracellular Ca^<2+> dynamics and gap junctional communication in injured hearts (in Japanese) (2007)
  • Author(s): Tanaka, H., Ovamada, M., Tsujii, E., Mani, H., Nakagami, T., Fujiwara, K., Takamatsu, T
  • Journal Title: Jpn J Electrocardiology 27 Pages: 230-236
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 多光子CALIによる生細胞内での蛋白質機能の時空間選択的阻害 (2007)
  • Author(s): 小山田正人, 高松哲郎
  • Journal Title: 病理と臨床 25 Pages: 553-559
• [Journal Article] 傷害心のカルシウム動態異常とギャップ結合変化 (2007)
  • Author(s): 田中秀央, 小山田正人, 辻井英治, 万井弘基, 中上拓男, 藤原克次, 高松哲郎
  • Journal Title: Jpn J Electrocardiology 27
• [Journal Article] 多光子CALIによる生細胞内での蛋白質機能の時空間選択的阻害 (2007)
  • Author(s): 小山田正人, 高松哲郎
  • Journal Title: 病理と臨床 (印刷中)
• [Journal Article] Gap junctions mediate glucose transport between GLUT1-positive and-negative cells in the spiral limbus of the rat cochlea (2006)
  • Author(s): Matsunami T, Suzuki T, Hisa Y, Takata K, Takamatsu T, Ovamaka M.
  • Journal Title: Cell Commun Adhes 13 Pages: 93-102
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Different changes in the expression of multiple kinds of tight-junction proteins during ischemia-reperfusion injury of the rat ileum (2006)
  • Author(s): Inoue K, Ovamada M, Mitsufuji S, Okanoue T, Takamatsu T.
  • Journal Title: Acta Histochem.Cytochem. 39 Pages: 35-45
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Acute aortic dissection presenting as a neurologic disorder (2006)
  • Author(s): Kawarabuki K, Sakakibara T, Hirai M, Shirasu M, Kohara I, Tanaka H, Ovamada M, Takamatsu T, Murayama Y, Yamaki T.
  • Journal Title: J Stroke Cerebrovasc Dis 15 Pages: 26-29
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] レーザーで生細胞内の機能分子を可視化する (2006)
  • Author(s): 小山田 正人, 高松 哲郎
  • Journal Title: レーザー研究 34 Pages: 807-812
  • NAID: 10018792460
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Different Changes in the Expression of Multiple Kinds of Tight-Junction Proteins during Ischemia-Reperfusion Injury of the Rat Ileum (2006)
  • Author(s): Inoue, K,. Ovamada, M., Mitsufuji, S., Okanoue, T., Takamatsu, T
  • Journal Title: Acta Histochem Cytochem 39 Pages: 35-45
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Acute aortic dissection presenting as a neurologic disorder (2006)
  • Author(s): Kawarabuki, K., Sakakibara, T., Hirai, M., Shirasu, M., Kohara, I., Tanaka, H., Ovamada, M., Takamatsu, T., Murayama, Y., Yamaki, T
  • Journal Title: J Stroke Cerebrovasc Dis 15 Pages: 26-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gap junctions mediate glucose transport between GLUT1-positive and -negative cells in the spiral Embus of the rat cochlea (2006)
  • Author(s): Matsunami, T., Suzuki, T., Hisa, Y., Takata, K., Takamatsu, T., Ovamada, M
  • Journal Title: Cell Commun Adhes 13 Pages: 93-102
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Laser Imaging of Functional Molecules in the Living Cell (in Japanese) (2006)
  • Author(s): Ovamada, M., Takamatsu, T
  • Journal Title: The Review of Laser Engineering 34 Pages: 807-812
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gap junctions mediate glucose transport between GLUT1-positive and -negative cells in the spiral limbus of the rat cochlea (2006)
  • Author(s): T.Matsunami, T, T.Suzuki, Y.Hisa, K.Takata, T.Takamatsu, M.Oyamada
  • Journal Title: Cell Commun. Adhes. 13 Pages: 93-102
• [Journal Article] Different changes in the expression of multiple kinds of tight- junction proteins during ischemia-reperfusion injury of the rat ileum (2006)
  • Author(s): K.Inoue, M.Oyamada, S.Mitufuji, T.Okanoue, T.Takamatsu
  • Journal Title: Acta Histochem. Cytochem. 39 Pages: 35-45
  • NAID: 110006203390
• [Journal Article] レーザーで生細胞内の機能分子を可視化する (2006)
  • Author(s): 小山田正人, 高松哲郎
  • Journal Title: レーザー研究 34 Pages: 807-812
  • NAID: 10018792460
• [Journal Article] Gap junctions mediate glucose transport between GLUT1-positive and -negative cells in the spiral limbus of the rat cochlea (2006)
  • Author(s): Matsunami T et al.
  • Journal Title: Cell Commun Adhes (印刷中)
• [Journal Article] Different changes in the expression of multiple kinds of tight-junction proteins during ischemia-reperfusion iniury of the rat ileum (2006)
  • Author(s): Inoue K et al.
  • Journal Title: Acta Histochem Cytochem (印刷中)
• [Journal Article] Multiphoton excitation-evoked chromophore-assisted laser inactivation using green fluorescent protein (2005)
  • Author(s): Tanabe T, Ovamada M, Fujita K, Dai P, Tanaka H, Takamatsu T
  • Journal Title: Nature Methods 2 Pages: 503-505
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Regulation of connexin expression (2005)
  • Author(s): Oyamada M, Oyamada Y, Takamatsu T.
  • Journal Title: Biochimica et Biophysica Acta-Biomembranes 1719 Pages: 6-23
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] In situ Ca^<2+>dynamics of Purkinje fibers and its interconnection with subjacent ventricular myocytes (2005)
  • Author(s): Hamamoto T, Tanaka H, Mani H, Tanabe T, Fujiwara K, Nakagami T, Horie M, Oyamada M, Takamatsu T.
  • Journal Title: J Mol Cell Cardiol 38 Pages: 561-569
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] ギャップ結合と病態発生 (2005)
  • Author(s): 小山田 正人, 小西 英一, 小山田 ゆみ子
  • Journal Title: 細胞 37 Pages: 29-33
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] excitation-evoked chromophore-assisted laser inactivation using green fluorescent protein (2005)
  • Author(s): Tanabe, T., Ovamada, M., Fujita, K., Dai, P., Tanaka, H., Takamatsu, T., Multiphoton
  • Journal Title: Nat Methods 2 Pages: 503-505
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Regulation of connexin expression Biochim Biophys Acta (2005)
  • Author(s): Ovamada, M., Oyamada, Y., Takamatsu, T
  • Journal Title: Biomembranes 1719 Pages: 6-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In situ Ca^<2+> dynamics of Purkinje fibers and its interconnection with subjacent ventricular myocytes (2005)
  • Author(s): Hamamoto, T., Tanaka, H., Mani, H., Tanabe, T., Fujiwara, K., Nakagami, T., Horie, M., Ovamada, M., Takamatsu, T
  • Journal Title: J Mol Cell Cardiol 38 Pages: 561-569
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gap Junctions and Human Disease (in Japanese) (2005)
  • Author(s): Ovamada, M., Konishi, E., Oyamada, Y
  • Journal Title: Saibou 37 Pages: 29-33
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Multiphoton excitation-evoked chromophore-assisted laser inactivation using green fluorescent protein (2005)
  • Author(s): Tanabe T et al.
  • Journal Title: Nature Methods 2 Pages: 503-505
• [Journal Article] Regulation of connexin expression (2005)
  • Author(s): Oyamada M et al.
  • Journal Title: Biochimica et Biophysica Acta - Biomembranes 1719 Pages: 6-23
• [Journal Article] In situ Ca^<2+> dynamics of Purkinje fibers and its interconnection with subjacent ventricular myocytes (2005)
  • Author(s): Hamamoto T et al.
  • Journal Title: J Mol Cell Cardiol 38 Pages: 561-569
• [Journal Article] ギャップ結合と病態発生 (2005)
  • Author(s): 小山田正人, 他
  • Journal Title: 細胞 37 Pages: 29-33
• [Presentation] 梗塞心における細胞間コミュニケーション-非心筋細胞に注目して (2007)
  • Author(s): 小山田 正人、谷口 大吾、藤原 克次、中上 拓男、原田 義規、田中 秀央、高松 哲郎
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Connexin43 knockdownによる角膜内皮損傷後の治癒促進とEMT抑制 (2007)
  • Author(s): 中野 由起子、小山田 正人、戴 平、高松 哲郎
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 多光子CALIによる内在性コネキシンの機能阻害条件の検討 (2007)
  • Author(s): 山岡 禎久、戴 平、人見 敏明、田中 秀央、小山田 正人、高松 哲郎
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Acceleration of wound healing and inhibition of mesenchymal transitionafter corneal endothelial injury in vivo by connexin43 knockdown (2007)
  • Author(s): Nakano Y, Ovamada M, Dai P, Kinoshita S, Takamatsu T.
  • Organizer: Gap Junction Conference 2007
  • Place of Presentation: Elsinore,Denmark
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Spatiotemporal inhibition of gap-junctional communication by multiphoton excitation-evoked chromophore-assisted laser inactivation(MP-CALI) (2007)
  • Author(s): Oyamada M, Tanabe T, Yamaoka Y, Dai P, Tanaka H, Takamatsu T.
  • Organizer: Gap Junction Conference 2007
  • Place of Presentation: Elsinore,Denmark
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Connexin43 knockdownによる角膜内皮損傷後の治癒促進とEMT抑制 (2007)
  • Author(s): 小山田 正人、中野 由起子、戴 平、中上 拓男、高松 哲郎
  • Organizer: 第6回コネキシン研究会
  • Place of Presentation: 秋田
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Acceleration of wound healing and inhibition of mesenchymal transition after corneal endothelial injury in vivo by connexin43 knockdown (2007)
  • Author(s): Nakano, Y., Ovamada, M., Dai, P., Kinoshita, S., Takamatsu, T
  • Organizer: Gap Junction Conference
  • Place of Presentation: Elsinore, Denmark
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Spatiotemporal inhibition of gap-junctional communication by multiphoton excitation-evoked chromophore -assisted laser inactivation (MP-CALI) (2007)
  • Author(s): Ovamada, M., Tanabe, T., Yamaoka, Y., Dai, P., Tanaka, H., Takamatsu, T.
  • Organizer: Gap Junction Conference
  • Place of Presentation: Elsinore, Denmark
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Acceleration of wound healing and inhibition of mesenchymal transition after corneal endothelial injury in vivo by connexin43 knockdown. (2007)
  • Author(s): Nakano Y, Oyamada M, Dai P, Kinoshita S, Takamatsu T.
  • Organizer: Gap Junction Conference 2007.
  • Place of Presentation: デンマーク、エルシノア市
• [Presentation] Spatiotemporal inhibition of gap-junctional communication by multiphoton excitation-evoked chromophore-assisted laser inactivation(MP-CALI). (2007)
  • Author(s): Oyamada M, Tanabe T, Yamaoka Y, Dai P, Tanaka H, Takamatsu T.
  • Organizer: Gap Junction Conference 2007
  • Place of Presentation: デンマーク、エルシノア市
• [Presentation] 傷害心筋におけるカルシウム動態の異常とギャップ結合の変化(シンポジウム) (2006)
  • Author(s): 田中 秀央、小山田 正人、辻井 英治、万井 広基、藤原 克次、中上 拓男、高松 哲郎
  • Organizer: 第23回日本心電学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2006-07-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 心筋のカルシウム動態と光学的膜電位のin situ同時可視化 (2006)
  • Author(s): 田中 秀央, 藤原 克次, 中上 拓男, 万井 弘基, 小山田 正人, 高松哲郎
  • Organizer: 第32回レーザー顕微鏡研究会講演会
  • Place of Presentation: 和光
  • Year and Date: 2006-06-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Gap junctions and cell death:Changes in connexin localization during apoptosis using cells expressing Cx43-EGFP(シンポジウム) (2006)
  • Author(s): Ovamada M, Zhou W, Oyamada Y, Takamatsu T.
  • Organizer: 第83回日本生理学会大会
  • Place of Presentation: 前橋
  • Year and Date: 2006-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] SEAO400,a selective inhibitor of sodium-calcium exchanger prevents contraction band formation of the heart induced by calcium paradox (2006)
  • Author(s): Maui H, Tanaka H, Tanabe T, Nakagami T, Fujiwara K, Ovamada M, Takalnatsu T.
  • Organizer: 第70回日本循環器学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ギャップ結合チャネルの構造と病態発生(ワークショップ) (2006)
  • Author(s): 小山田 正人、高松 哲郎
  • Organizer: 第95回日本病理学会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 心筋の収縮帯形成機序の検討 (2006)
  • Author(s): 万井 弘基, 田中 秀央, 小山田 正人, 高松 哲郎
  • Organizer: 第95回日本病理学会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] In situ confocal visualization of intracellular calcium dynamics at the Purkinje-ventricular interconnection of the Langendorff-perfused rat heart (2006)
  • Author(s): Fujiwara K, Tanaka H, Hamamoto T, Maui H, Nakagami T, OvamadaM, Takamatsu T
  • Organizer: World Congress of Cardiology
  • Place of Presentation: Barcelona,Spain
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Simultaneous imaging of the single-myocyte optical action potentials and intracellular Ca2+ dynamics in Langendorff-perfused rat heart by a novel dual-imaging confocal microscopy (2006)
  • Author(s): Fujiwara K, Tanaka H, Nakagami T, Mani H, Tanabe T, Oyamada M, Takamatsu T
  • Organizer: Scientific Sessions 2006, American Heart Association
  • Place of Presentation: Chicago,USA
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Analyses of abnormalities in gap junctions in heart disease by immunohistochemistry,in situ Ca^<2+> imaging and MP-CALI(invited talk) (2006)
  • Author(s): Ovamada M, Tanaka H, Takamatsu T.
  • Organizer: International Conference:Physiological and Pathological Importance of Gap Junctions
  • Place of Presentation: Tokyo
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Roles of gap junctions in glucose transport between GLUT1-positive and-negative cells in the rat cochlea (2006)
  • Author(s): Suzuki T, Matsunami T, Hisa Y, Takata K, Takamatsu T, Ovamada M.
  • Organizer: International Conference:Physiological and Pathological Importance of Gap Junctions
  • Place of Presentation: Tokyo
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] In situ confocal visualization of intracellular calcium dynamics at the Purkinje-ventricular interconnection of the Langendorff-perfused rat heart (2006)
  • Author(s): Fujiwara, K., Tanaka, H., Hamamoto, T., Mani, H., Nakagami, T., Ovamada, M., Takamatsu, T
  • Organizer: World Congress of Cardiology
  • Place of Presentation: Barcelona, Spain
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Simultaneous imaging of the single-myocyte optical action potentials and intracellular Ca2+ dynamics in Langendorff-perfused rat heart by a novel dualimaging confocal microscopy (2006)
  • Author(s): Fujiwara, K., Tanaka, H., Nakagami, T., Mani H., Tanabe, T., Ovamada, M., Takamatsu, T
  • Organizer: Scientific Sessions 2006 American Heart Association
  • Place of Presentation: Chicago, USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Analyses of abnormalities in gap junctions in heart disease by immunohistochemistry, in situ Ca^<2+> imaging and MP-CALI (2006)
  • Author(s): Ovamada, M., Tanaka, H., Takamatsu, T
  • Organizer: International Conference: Physiological and Pathological Importance of Gap Junctions
  • Place of Presentation: Tokyo
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Roles of gap junctions in glucose transport between GLUT1-positive and -negative cells in the rat cochlea (2006)
  • Author(s): Suzuki, T., Matsunami, T., Hisa, Y., Takata, K., Takamatsu, T., Ovamada, M
  • Organizer: International Conference: Physiological and Pathological Importance of Gap Junctions
  • Place of Presentation: Tokyo
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Irreversible myocardial injury by Ca2+ paradox induces contraction band necrosis in the working heart by influx through the Na2+-Ca2+ exchange (2005)
  • Author(s): Mani H, Tanaka H, Tanabe T, Nakagami T, Fujiwara K, Dai P, Ovamada M, Takamatsu T.
  • Organizer: Scientific Sessions 2005 American Heart Association
  • Place of Presentation: Dallas,USA
  • Year and Date: 2005-11-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 2光子吸収CALIを用いた機能分子の制御(シンポジウム) (2005)
  • Author(s): 田邉 卓爾、小山田 正人、藤田 克昌、戴 平、田中 秀央、高松 哲郎
  • Organizer: 日本組織細胞化学会第46回総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2005-10-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ギャップ結合の異常と疾患(ワークショップ) (2005)
  • Author(s): 小山田 正人, 鈴木 敏弘, 高松 哲郎
  • Organizer: 日本組織細胞化学会第46回総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ラット回腸の虚血再還流障害におけるタイト結合蛋白質の発現変化 (2005)
  • Author(s): 井上 香織、小山田 正人、光藤 章二、岡上 武、高松 哲郎
  • Organizer: 日本組織細胞化学会第46回総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 蝸牛ラセン縁におけるギャップ結合蛋白質とグルコーストランスポーター1の局在 (2005)
  • Author(s): 鈴木 敏弘、小山田 正人、松波 達也、高松 哲郎
  • Organizer: 日本組織細胞化学会第46回総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ラット生体位心プルキンエ線維網におけるカルシウム波の特性 (2005)
  • Author(s): 田中 秀央、浜本 徹、万井 弘基、田辺 卓爾、藤原 克次、中上 拓男、小山田 正人、高松 哲郎
  • Organizer: 日本組織細胞化学会第46回総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 心筋細胞におけるギャップ結合蛋白(コネキシン43)とカルシウム動態の同時観察の試み (2005)
  • Author(s): 中上 拓男、田中 秀央、戴 平、田邉 卓爾、藤原 克次、万井 弘基、小山田 正人、高松 哲郎
  • Organizer: 日本組織細胞化学会第46回総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 光ピンセットを用いた一次繊毛の力学的特性の検討 (2005)
  • Author(s): 原田 義規、太田 泰輔、田中 秀央、小山田 正人、河田 聡、高松 哲郎
  • Organizer: 日本組織細胞化学会第46回総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ラット角膜内皮細胞の創傷治癒過程におけるギャップ結合蛋白質の発現変化と細胞増殖、ポンプ機能 (2005)
  • Author(s): 中野 由起子、小山田 正人、戴 平、高松 哲郎
  • Organizer: 日本組織細胞化学会第46回総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] コネキシン43遺伝子の細胞周期への影響 (2005)
  • Author(s): 人見 敏明、戴 平、中上 拓男、小山田 正人、高松 哲郎
  • Organizer: 日本組織細胞化学会第46回総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カルシウムパラドックスによる不可逆的心筋障害のメカニズム (2005)
  • Author(s): 万井 弘基、田中 秀央、中上 拓男、藤原 克次、小山田 正人、高松 哲郎
  • Organizer: 日本組織細胞化学会第46回総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Temporal altefations of connexin43 localization and mitochondrial membrane potential during ultraviolet light-induced apoptosis (2005)
  • Author(s): Ovamada M, Zhou W, Oyamada Y, Takamatsu T.
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 大宮
  • Year and Date: 2005-06-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 紫外線誘導アポトーシス過程におけるギャップ結合の局在とミトコンドリア膜電位の変化-Cx43-EGFP発現HeLa細胞を用いて (2005)
  • Author(s): 小山田 正人, 周武 雄, 小山田 ゆみ子, 高松 哲郎
  • Organizer: 第94回日本病理学会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 細胞解離過程におけるギャップ結合蛋白コネキシンの動態 (2005)
  • Author(s): 小山田 ゆみ子、小山田 正人、高松 哲郎
  • Organizer: 第94回日本病理学会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 非腫瘍肝組織に広範な線維化を伴う胆管増生を呈した肝細胞癌例-増生胆菅のoriginの検討 (2005)
  • Author(s): 小山田 ゆみ子、小山田 正人、原田 義規、高松 哲郎
  • Organizer: 第94回日本病理学会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 心室内電導障害におけるプルキンエ線維-心室筋節合部のCa2+動態異常 (2005)
  • Author(s): 田中 秀央、浜本 徹、小山田 正人、高松 哲郎
  • Organizer: 第94回日本病理学会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Changes in connexin43 locahzation during ultraviolet light-induced apoptosis in HeLa cells expressing Cx43-EGFP:Comparison with mitochondrial membrane potential (2005)
  • Author(s): Ovamada M, Zhou W, Oyamada Y, Takamatsu T:
  • Organizer: Gap Junction Conference 2005
  • Place of Presentation: Whistler,Canada
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Formation of tubular structures containing gap-junction plaques during cell dissociation by trypsinization revealed by time-lapse imaging of cells expressing connexin-GFP (2005)
  • Author(s): Oyamada Y, Takamatsu T, Ovamada M
  • Organizer: Gap Junction Conference 2005
  • Place of Presentation: Whistler,Canada
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Gap junctions between GLUT1-positive and-negative cells in the cochlea:Their possible roles in intercellular transport of glucose over the blood-inner ear barrier (2005)
  • Author(s): Suzuki T, Matsunami T Hisa Y, Takata K, Takamatsu T, Ovamaka M
  • Organizer: Gap Junction Conference 2005
  • Place of Presentation: Whistler,Canada
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Young's modulus of primary ciia measured with optical tweezers (2005)
  • Author(s): Harada Y, Ota T, Dai P, Tanaka H, Ovamada M, Kawata S, Takamatsu T.
  • Organizer: The American Society for Cell Biology 45th Annual Meeting
  • Place of Presentation: SanFrancisco,USA
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Multiphoton Excitation-evoked Chromophore-assisted Laser Inactivation Using Green Fluorescent Protein (2005)
  • Author(s): Tanabe T, Ovamaka M, Fujita K, Dai P, Tanaka H, Takamatsu T:
  • Organizer: The American Society for Cell Biology 45th Annual Meeting
  • Place of Presentation: SanFrancisco,USA
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 多光子CALIを用いたギャップ結合細胞間コミュニケーションの時空間選択的阻害 (2005)
  • Author(s): 小山田 正人、田邉 卓爾、藤田 克昌、戴 平、田中 秀央、高松 哲郎
  • Organizer: 第4回コネキシン研究会
  • Place of Presentation: 京都
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ラット角膜内皮細胞の創傷治癒過程におけるギャップ結合蛋白質の発現 (2005)
  • Author(s): 中野 由起子、小山田 正人、戴 平、高松 哲郎
  • Organizer: 第4回コネキシン研究会
  • Place of Presentation: 京都
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 蝸牛内細胞間グルコース輸送におけるギャップ結合の役割 (2005)
  • Author(s): 鈴木 敏弘、松波 達也、久 育男、小山田 正人、高松 哲郎
  • Organizer: 第4回コネキシン研究会
  • Place of Presentation: 京都
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Changes in connexin43 localization during ultraviolet light-induced apoptosis in HeLa cells expressing Cx43-EGFP: Comparison with mitochondrial membrane potential (2005)
  • Author(s): Ovamada, M., Zhou, W., Oyamada, Y., Takamatsu, T
  • Organizer: Gap Junction Conference 2005
  • Place of Presentation: Whistler, British Columbia, Canada
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Formation of tubular structures containing gap-junction plaques during cell dissociation by trypsinization revealed by time-lapse imaging of cells expressing connexin-GFP (2005)
  • Author(s): Oyamada, Y., Takamatsu, T., Ovamada, M
  • Organizer: Gap Junction Conference 2005
  • Place of Presentation: Whistler,British Columbia, Canada
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Gap junctions between GLUT1-positive and -negative cells in the cochlea: Their possible roles in intercellular transport of glucose over the blood-inner ear barrier (2005)
  • Author(s): Suzuki ,T., Matsunami, T, Hisa, Y., Takata, K., Takamatsu, T., Ovamada, M
  • Organizer: Gap Junction Conference 2005
  • Place of Presentation: Whistler, British Columbia, Canada
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Irrevresible myocardial injury by Ca^<2+> paradox induces contraction band necrosis in the working heart by influx through the Na-Ca^<2+> exchange (2005)
  • Author(s): Mani, H., Tanaka, H., Tanabe, T., Nakagami, T., Fujiwara, K., Dai P, Ovamada, M., Takamatsu
  • Organizer: Scientific Sessions 2005 American Heart Association
  • Place of Presentation: Dallas, Texas, USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Young's modulus of primary cilia measured with optical tweezers (2005)
  • Author(s): Harada, Y., Ota, T., DaiM, P., Tanaka, H., Ovamada, M., Kawata, S., Takamatsu, T
  • Organizer: The American Society for Cell Biology 45th Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Multiphoton Excitation-evoked Chromophore -assisted Laser Inactivation Using Green Fluorescent Protein (2005)
  • Author(s): Tanabe, T., Ovamada, M., Fujita, K., Dai, P., Tanaka, H., Takamatsu, T
  • Organizer: The American Society for Cell Biology 45th Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Description: 「研究成果報告書概要(欧文)」より