New optical brain imaging in infants by using near-infrared diffuse optical tomography

• Project/Area Number: 17390307
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Kagawa University
• Principal Investigator: KUSAKA Takashi Kagawa University, Maternal Perinatal Center, Faculty of Medicine, Maternal Perinatal Center (50274288)
• Co-Investigator(Kenkyū-buntansha): YAMADA Yukio University of Electro-Communications, Dept. of Mech. Eng. and Intel. Sys, Japan Professor (10334583) ; TANIKAWA Yukari National Institute of Advanced Industrial Science and Technology(AIST), 人間福祉医工学研究部門, Japan (20344202) ; NISHIDA Tomoko Kagawa University, Dept of Pediatrics, Assistant Professor (00243759) ; NAMBA Masanori Kagawa University, Dept of Pediatrics, Japan. Assistant Professor (90237636) ; OKUBO Kensuke Kagawa University, Dept of Pediatrics, Assistant Professor (80335851)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥9,100,000 (Direct Cost: ¥9,100,000); Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000); Fiscal Year 2005: ¥6,700,000 (Direct Cost: ¥6,700,000)
• Keywords: Near-infrared spectroscopy / Optical tomography / Infant / Hypocapnea / Cerebral blood volume / Periventricular leukomalasia / Time-resolved spectroscopy / Cerebral Hb oxygen saturation / 低出生体重児 / 脳内血液量 / 脳内ヘモグロビン酸素飽和度 / 吸収係数 / 散乱係数 / トモグラフィー / 新生児 / 脳 / 組織Hb酸素飽和度

Research Abstract

An optical 3D imaging method using near-infrared diffuse optical tomography (NIR DOT) to estimate the regional light scattering and absorption properties in infants has been developed. The NIR DOT system is consisted of 16 source-detector fibers, pulsed diode lasers operating at the wavelengths of 759 and 835 nm and time correlated single photon counting units. The image reconstruction algorithm is based on the modified generalized pulsed spectral technique. The time-resolved data were used to estimate for concentration of oxyHb and deoxyHb.
DOT was measured in an infant on 29 days after birth, whose gestational age and birth weight was 23 weeks and 658g, assisted by an artificial respirator. Informed consent in writing was obtained from the parents, and the study was approved by the local ethics committee.
The data under two different state, (A) normocapnea and (B) hypocapnea induced by hyperventilation, were obtained. DOT images showed the Hb concentrations obtained at the state (B) subtracted by (A). The results showed that the total Hb concentration in the most parts of the brain decreased, reflecting the decrease in cerebral blood flow, especially white matter regions. The results demonstrated that NIR DOT is capable of reconstructing the images of Hb concentration with a high spatial resolution in infants at the bedside.

Research Products

• [Journal Article] Relationship between muscle oxygenation and electromyography activity during sustained isometric contraction (2008)
  • Author(s): Yamada E, Kusaka T, Arima N, Isobe K, Yamamoto T, Itoh S.
  • Journal Title: Clinical Physiology and Functional Imaging (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Relationship between muscle oxygenation and electromyography activity during sustained isometric contraction. (2008)
  • Author(s): Yamada E, Kusaka T, Arisna N, Isobe H, Yamasnoto T, Itoh S.
  • Journal Title: Clinical Physialogy and Functionsi Imaging (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Accumulation of triosephosphate is.omerase,with sequence homology to amyloid peptides,in vessel walls of the newborn piglet hippocampus (2007)
  • Author(s): Kusaka T, Ueno M, et. al.
  • Journal Title: Microscopy Research and Technique Jul;70(7) Pages: 648-55
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Functional optical hemodynamic imaging of the olfactory cortex (2007)
  • Author(s): Kobayashi E, Kusaka T, Karaki M, et. al.
  • Journal Title: Laryngoscope Mar;117(3) Pages: 541-6
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The effect of prenatal X-irradiation on the developing cerebral cortex of rats.II:A quantitative assessment of glial cells in the somatosensory cortex (2007)
  • Author(s): Miki T, Yokoyama T, Sumitani K, Wang ZY, Yang W, Kusaka T, et. al.
  • Journal Title: International Journal of Developmental.Neuroscience Aug;25(5) Pages: 293-7
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] In vitro production of bilirubin photoisomers by light irradiation using neoBLUE (2007)
  • Author(s): Okada H, Abe T, Etoh Y, Yoshino S, Kato I, Iwaki T, Okubo K, Yasuda S, Kawada K, Kusaka T, et. al.
  • Journal Title: Pediatr Int. Jun;49(3) Pages: 318-21
  • NAID: 10019493781
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 拡散光トモグラフィ装.置を用いた未熟児頭部のin vivo測定 (2007)
  • Author(s): 上野 雅範, 福沢 遼, 大川 晋平, 山田 幸生, 日下 隆, 西田 智子, 磯部 健一, 谷川 ゆかり, 高 峰, 佐藤 知絵, 星 詳子
  • Journal Title: 第19回バイオエンジニアリン「グ講演会講演論文集 Pages: 338-339
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] In vivo measurement of premature neonato head using diffuse optical tomography. (2007)
  • Author(s): Masanori Ueno, Ryo Fukuzawa, Shinpei Okawa, Yultio Yamada, Takasbi Kusaka, Tomoko Nisbida, Kenichi Isobe, Yukaxi Tanikawa, Feng Gao, Chie Sato, Yoko Hoshi.
  • Journal Title: Proceedings of the 19th Bioengineering Conference (in Japanese) Pages: 338-339
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In vivo measurement of premature neonate head using diffuse optical tomography. (2007)
  • Author(s): Masanori Ueno, Ryo Fukuzawa, Shinpei Okawa, Yukio Yasnada, Takashi Kusaka, Tomoko Nishida, Kenichi bobs, Yukari Tanikawa, Feng Gao, Cbie Sato, Yoko Hoshi.
  • Journal Title: 3rd Asian and Pacific Rim Sympoaium an Biophotonics & Biophetonica Downunder II, Besk of Abstract Pages: 43-44
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Study for diffuse optical tomographyby using nearinfrared time-resolved method. Dept. of Mech. Rug. and Intel. (2007)
  • Author(s): Masanori Ueno.
  • Journal Title: Sys., Graduate school of Universiiy of Riectro-Communicatisna Japan. A graduation thesis (in Japanese)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Study for diffuse optical tomography in premature infant. Dept of Mech. Rug. end Intel. (2007)
  • Author(s): Ryo Fukuzawa.
  • Journal Title: Sys., University of Electro-Commusicatione, Japan. A graduation thesis (in Japanese)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Accumulation of triosephosphate isomerass, with sequence homology to amyloid peptides, in vessel walls of the newborn piglet hippocampue. (2007)
  • Author(s): Kusaka T, Ueno M, Mulci T, Kanenishi K, Nagai Y, Huang CL, Okamoto Y, Ogawa T, Onodera hi, Itoh S, Altiguchi I, Sakamoto H.
  • Journal Title: Microscopy Research and Technique 70(7) Pages: 648-55
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional optical hemodynarnic imaging of the olfactory cortex. (2007)
  • Author(s): Kobayasbi E, Kusaka T, Karaki M, Kobayashi R, Itoh S, Men N.
  • Journal Title: Laryngoscope 117(3) Pages: 541-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The effect of prenatal lliirradiation on the developing cerebral cortex of rate. II : A quantitative assessment of glial cells in the somatosensory cortex. (2007)
  • Author(s): Multi T, Yokoyama T, Sumitani K, Wang ZY, Yang W, Kusaka T, Matsumeto Y, Warita K, Lee NS, Fukui Y, Takeuchi Y, et. al.
  • Journal Title: International Journal of Developmental Neurescience. 25(5) Pages: 293-7
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In vitro production of bilirubin photaisomers by light ircadiation using neoBLUE. (2007)
  • Author(s): Okada H, Abe T, Etoh Y, Yosbino S, Kate I, Iwaki T, Okubo K, Yasuda S, Kawada K, Kusaka T, Nemba M, Nisbitla T, Imai T, Isobe K, Itoh S.
  • Journal Title: irediatr tnt. 49(3) Pages: 318-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 拡散光トモグラフィ装置を用いた未熟児頭部のin vivo測定 (2007)
  • Author(s): 上野雅範, 福沢遼, 大川晋平, 日下隆 等
  • Journal Title: 第19回バイオエンジニアリング講演会講演論文集 06-05 Pages: 338-9
• [Journal Article] Accumulation of triosephosphate isomerase, with sequence homology to beta amyloid peptides, in vessel walls of the newborn piglet hippocampus. (2007)
  • Author(s): Kusaka T, Ueno M, Miki T, et al.
  • Journal Title: Microsc Res Tech (In press)
• [Journal Article] Functional optical hemodynamic imaging of the olfactory cortex (2007)
  • Author(s): Kobayashi E, Kusaka T, Karaki M, et al.
  • Journal Title: Laryngoscope 117(3) Pages: 541-6
• [Journal Article] Developmental changes of optical properties in neonates determined by near-infrared time-resolved spectroscopy. (2005)
  • Author(s): Ijichi S, Kusaka T, Isobe K, et al.
  • Journal Title: Pediatric Research 58 Pages: 568-573
• [Journal Article] Quantification of cerebral hemoglobin as a function of oxygenation using near-infrared time-resolved spectroscopy in a piglet model of hypoxia. (2005)
  • Author(s): Ijichi S, Kusaka T, Isobe K, et al.
  • Journal Title: Journal of Biomedical Optics 10 Pages: 24026-24026